海带中细胞激动素的纯化分离方法   总被引：1，自引：0，他引：1  

黄冰心  范晓  韩丽君 《海洋与湖沼》2002,33(5):509-514

海带样品于 2 0 0 1年采自青岛太平角海湾 ,用三种不同方法处理样品 ,通过对实验方法及条件的筛选 ,确立了提取、纯化和分离海带中细胞激动素的方法步骤和流程。结果表明 ,最佳方法的基本步骤为 :( 1 )用甲醇∶水∶醋酸 ( 70∶ 30∶ 3,V/V/V)从海带组织中提取出细胞激动素 ,再用PVPP柱去除提取液中的酚类和其他杂质 ;( 2 )将提取液通过连在一起的PVPP柱、DEAE柱和C1 8柱 ,以纯化样品并同时从自由基、核苷、核苷酸和葡糖苷型细胞激动素混合物中分离出核苷酸型细胞激动素 ;( 3)用接有氨基柱的正相液相色谱进一步将葡糖苷型细胞激动素分离出来 ;( 4 )用C1 8液相色谱柱分离其他各种细胞激动素  相似文献   

Effects of an Artificial Breakwater on the Distributions of Planktonic Microbial Communities     

Young-Ok Kim  Eun Jin Yang  Jung-Hoon Kang  Kyoungsoon Shin  Man Chang  Cheol Soo Myung 《Ocean Science Journal》2007,42(1):9-17

The summer distributions of planktonic microbial communities (heterotrophic and phtosynthetic bacteria, phtosynthetic and heterotrophic nanoflagellates, ciliate plankton, and microphytoplankton) were compared between inner and outer areas of Lake Sihwa, divided by an artificial breakwater, located on the western coast of Korea, in September 2003. The semienclosed, inner area was characterized by hyposaline surface water (<17 psu), and by low concentrations of dissolved oxygen (avg. 0.4 mg L¹) and high concentrations of inorganic nutrients (nitrogenous nutrients >36 μM, phosphate <4 μM) in the bottom layer. Higher densities of heterotrophic bacteria and nanoflagellates also occurred in the inner area than did in the outer area, while microphytoplankton (mainly diatoms) occurred abundantly in the outer area. A tiny tintinnid ciliate, Tintinnopsis nana, bloomed into more than 10⁶ cells L¹ at the surface layer of the inner area, while its abundance was much lower (10³-10⁴ cells L¹) in the outer area of the breakwater. Ciliate abundance was highly correlated with heterotrophic bacteria (r = 0.886, p < 0.001) and heterotrophic flagellates (r = 0.962, p < 0.001), indicating that rich food availability may have led to theT. nana bloom. These results suggest that the breakwater causes the eutrophic environment in artificial lakes with limited flushing of enriched water and develops into abundant bacteria, nanoflagellates, and ciliates.  相似文献   

Strength Analysis of Turret Mooring Productive/Storage Tanker     

Zhang Shilian Jin Dexian Associate Professor 《中国海洋工程》1997,(3)

The longitudinal strength of turret mooring productive/storage tanker is studied.A numeri-cal example has been implemented according to the method presented in this paper to give practical il-lustration.From the results of the numerical example,it is concluded that the turret hole located near theforward of the amidships has small effect on the longitudinal strength of the ship hull.As for design ex-treme value of wave bending moment of storage tanker,statistic method is a more reasonablemethodology,especially with the consideration of the servere environmental conditions.The primary esti-mation of design section modulus of turret storage tanker can be determined by this design bending mo-ment.  相似文献   

四川椎实螺属一新种(肺螺亚纲:基眼目,椎实螺科)     

金志良  刘月英 《海洋科学集刊》1997,(39):9-14

作者1987年于四川省乐山市大渡河尾段发现椎实螺属一新种,现命名为乐山椎实螺(Lymnaea leshanensis sp.nov.)。该种与日本琵琶湖产的 Lymnaea onychia 相似,但贝壳的形态、齿式、齿形、两性生殖系统的诸器官、外套膜的色带、个体大小、生活环境和动物区系均有所不同。前者属东洋区系,后者属古北区系。  相似文献   

Prediction of Load Carrying Capacity of Corroded Reinforced Concrete Beam     

范颖芳  周晶冯新 《中国海洋工程》2004,18(1):107-118

A novel method for prediction of the load carrying capacity of a corroded reinforced concrete beam (CRCB) is presented in the paper. Nine reinforced concrete beams, which had been working in an aggressive environment for more than 10 years, were tested in the laboratory. Comprehensive tests, including flexural test, strength test for corroded concrete and rusty rebar, and pullout test for bond strength between concrete and rebar, were condueted. The flexural test results of CRCBs reveal that the distribution of surface cracks on the beams shows a fractal behavior. The relationship between the fractal dimensions and mechanical properties of CRCBs is then studied. A prediction model based on artificial neural network (ANN) is established by the use of the fractal dimension as the corrosion index, together with the basic intbrmation of the beam. The validity of the prediction model is demonstrated through the experimental data, and satisfactory resuits are achieved.  相似文献   

吡喹酮和克螨特复配驱虫制剂对一些水生饵料生物的毒性     

樊海平 《台湾海峡》2004,23(4):500-506

由吡喹酮和克螨特组成的复配制剂对小球藻、轮虫、蚤状溞、水蚯蚓和卤虫的毒性实验结果显示：复配制剂对轮虫的半致死浓度(LC50)为13．9mg／dm^3(24h)和3．03mg／dm^3(48h)．安全浓度(SC)为0．0432mg／dm^3；对蚤状溞的LC50为345．14mg／dm^3(24h)和17．5mg／dm^3(48h)，SC为0．0135mg／dm^3；对水蚯蚓的LC50为636．80mg／dm^3(24h)和79．07mg／dm^3(48h)，SC为0．366mg／dm^3；对卤虫无节幼虫的LC50为201．84mg／dm^3(24h)和8．91mg／dm^3(48h)．SC为0．00519mg／dm^3,复配制剂对水环境生物的破坏作用小．  相似文献   

水样及生物体内微量锌、锰、硒的极谱催化波同时测定   总被引：1，自引：0，他引：1  

刘爱骅  王瑾  杨孙楷 《台湾海峡》1996,(3)

本文提出了在酒石酸盐-乙二胺-高氯酸-亚硫酸钠-高碘酸钾-EDTA体系中同时测定微量锌、锰、硒的极谱催化波，波峰敏锐，波形稳定。Zn2 浓度在0．4～40μg／dm3，Mn2 浓度在1．6～80μg／dm3，Se（Ⅳ）浓度在0．02～3．0μg／dm3范围内浓度与峰电流呈线性关系。取天然水样平行12份测定，变异系数在10％以内。  相似文献   

渤海、黄海强温跃层数值预报模式     

王宗山  徐伯昌  金梅兵  邹娥梅  李繁华 《海洋科学进展》1996,(3)

文中对前人关于水温垂直结构计算、模拟和预报研究成果进行了评价，并在此基础上建立了考虑海面吸收辐射和透射辐射、地形、风、海流、界面摩擦及其引起的混合作用的强温跃层三维数值预报模式。该模式运行的驱动量仅为风和气温场资料，因而具有较好的实用性；此外，从试报的结果看，效果是令人满意的。  相似文献   

波浪底摩擦力对重砂富集的影响——以鲁南近岸区为例     

金秉福 《海洋科学》1997,21(5):49-52

根据浅水波浪的底摩擦效应，对鲁南灵山湾和臼－岚山镇近岸区进行了波浪的海底摩擦力计算。波浪的海底摩擦力等值图与已知滨海锆石砂矿资料对比表明，底摩擦力大于０．１８Ｎ／ｍ＾２的动力分选区有利于重矿物砂富集。据此，进一步预测了鲁南近岸带的砂矿富集区。  相似文献   

单环刺螠(Urechis unicinctus)的人工苗种生产研究　Ⅰ .水温对胚胎发育及幼体培育的影响     

康庆浩  郑家声  金在敏 《中国海洋大学学报(自然科学版)》2002,32(2):273-278

为给单环刺 (U.unicinctus)的苗种生产提供有效的基础资料 ,本文研究了水温对单环刺胚胎发育的影响。结果表明 :单环刺各胚胎发育期的发育速度 (h)随水温 (T)升高而加快 ,有明显的正相关性。求得胚胎发育的生物学零度为平均 2℃。不同水温条件下 ,各期幼虫期的存活率以2 0℃为最高 ,培育水温高于或低于 2 0℃时生存率逐渐降低。幼虫培育适宜温度为 15～ 2 0℃。  相似文献