Isolation, cloning and sequencing of AFLP markers related to disease-resistance traits in Fenneropenaeus chinensis   总被引：1，自引：0，他引：1  

岳志芹  王伟继孔杰 戴继勋 《中国海洋湖沼学报》2005,23(4):442-447

Amplified fragment length polymorphisms （AFLP） technique was used to analyze the fingerprinting of four successive generations of Fenneropenaeus chinensis to reveal their disease-resistance traits. Some loci showed quite different genetic frequencies due to artificial selection, which implied that these fragments were putative markers related to the disease-resistance trait. We developed a simple and effective method to further characterize these AFLP fragments. Specific AFLP bands were cut directly from polyacrylamide gels, re-amplified, cloned and sequenced. Eight putative genetic markers were sequenced and their sizes ranged from 63 to 209 bp. The sequences were submitted to dbGSS （database of Genome Sequence Survey）; and the BLAST analysis showed low similarity to the function genes, indicating these markers were tightly linked to a disease-resistance trait but were not functional genes.  相似文献   

环渤海地区GPS台站时间序列分析   总被引：2，自引：1，他引：1  

邱亚辉  薄志毅  郎博  岳彩亚 《测绘通报》2018,(6):17-21

环渤海区域环境和气候复杂多变,随着地区和区域的不同,地质、地貌间的差异较大。探究该地区GPS台站时间序列的变化规律和台站的主要噪声类型、频谱规律显得尤为重要。本文基于该区域内所积累的连续数年运行的GPS测站数据,采用GAMIT/GLOBK软件进行解算和平差,获得每一个台站的坐标时间序列,并使用Lomb周期图法分析了该区域台站的频谱特性和周期特性。最后通过对频率和功率谱线性拟合分析研究了台站的噪声类型。此方法对台站时间序列去除噪声和进一步研究测站的运动规律提供了理论依据。  相似文献   

MVT矿床中与成矿有关热液方解石的地球化学判据——以青海多才玛超大型铅锌矿床为例     

吴畏  宋玉财  庄亮亮  黄世强  胡戈  黄钢  田力丹  岳龙龙 《岩石矿物学杂志》2023,42(1):47-70

碳酸盐矿物是MVT型铅锌矿床最为常见的热液蚀变矿物,记录了成矿流体特征,其元素或同位素组成对示踪和定位矿体具有一定的指示意义,但由于该类矿床中碳酸盐矿物成因多样,单一的岩相学观察往往难以准确识别与成矿有关和无关的方解石,需要辅以地球化学手段来确定方解石成因。本文利用钻孔编录、岩相学观察、C-O-Sr同位素和原位微量与稀土元素组成分析等手段,探讨了青海多才玛超大型MVT铅锌矿床中与成矿有关和无关两期方解石的矿物化学特征。结果表明,多才玛矿床成矿前方解石(Cal1)空间上与矿体无相关关系,遍布于中二叠统九十道班组灰岩内,阴极发光呈暗棕色至棕黄色,无明显生长环带;成矿期方解石(Cal2)主要产于铅锌矿体内及其上盘围岩中,多呈砂糖粒状-皮壳状充填于围岩裂隙和溶孔内,阴极发光呈暗黄色至亮橙色,常发育生长环带。相对于Cal2, Cal1与围岩的C-O-Sr同位素组成更为接近,这表明水岩反应期间,Cal1组成受围岩影响更大,Cal2组成受影响更小;相比于Cal1,Cal2具有较高的Mn、Fe、Zn、Pb含量和U/Th、V/Cr值以及较低的LREE/HREE值、Mg、Sr含量和弱的Ce负异常,指示成矿...  相似文献   

地震动记录选取及其对网架屋盖动力响应的影响          下载免费PDF全文

马风龙  乐风江  王健 《世界地震工程》2022,38(1):090-98

地震动记录的合理选取对预测结构响应有着重要的作用。本文通过对风雨操场建筑混合结构的抗震性能分析,提出了一种对水平及竖向地震动频谱特性均进行控制的改进选波方法。为了评价不同选波方法的可靠性和有效性,根据初选条件选取55组三向地震动记录,并以55组地震动的统计反应谱作为目标反应谱,以55组记录计算的结构响应均值作为"预测值",通过与单周期点和双频段选波方法的计算结果对比,分析表明:改进选波方法计算的结构基底剪力、柱顶位移、支座位移和网架竖向位移的相对误差和变异系数均小于前两种选波方法,其计算结果更加可靠有效。  相似文献   

超大沉井基础取土下沉刃脚土压力变化规律研究     

蒋凡  刘华  岳青  杨文爽 《岩土力学》2022,43(Z2):431-442

依托常泰长江大桥主塔沉井基础工程,采用三维有限元方法,模拟了大型沉井首次取土下沉阶段刃脚土压力的变化过程,并结合现场刃脚土压力实测数据,分析了沉井下沉工序对刃脚土压力分布的影响以及取土过程中刃脚土压力的变化规律。现场监测结果表明：刃脚实际土压力变化规律基本上佐证了数值模拟结果。井孔内取土导致取土区域沉井刃脚处土压力下降,取土区域刃脚土压力随取土厚度的增大而逐渐降低,土体压应力转移至尚未取土区域的刃脚处。在由内井孔向外井孔区域取土的过程中,刃脚土压力向外井孔刃脚区域转移,导致外井壁和外隔墙区域刃脚土压力逐渐增大,直至达到其极限承载力,外井壁区域土体进入塑性状态,沉井出现明显下沉。给出的沉井刃脚处土压力的变化规律可为同类大型沉井可控下沉提供指导。  相似文献   

武汉钰龙金融广场超深基坑工程设计方案选型          下载免费PDF全文

杜魁  余小国  岳丽娜  李超 《探矿工程》2015,42(12):28-33

以武汉钰龙金融广场超深基坑工程为实例,在结合常用的深基坑设计方案的基础上分别从基坑总体设计方案选定、基坑围护体系选定和设计、支撑体系选定、基坑降水选定、土方开挖方案选型和换撑方案的选定等方面进行探讨,并确定最终的基坑工程设计方案选型,即地下连续墙围护+3道内支撑+管井降水处理承压水的支护方案。  相似文献   

A New Species of Rhodoleia (Hamamelidaceae) from the Upper Pliocene of West Yunnan, China and Comments on Phytogeography and Insect Herbivory     

WU Jingyu  ZHAO Zhenrui  LI Qiji  LIU Yusheng 《《地质学报》英文版》2015,89(5):1440-1452

In Europe, fossil fruits and seeds of Rhodoleia (Hamamelidaceae) have been described from the Upper Cretaceous to the Miocene, whereas no fossil record of Rhodoleia has been reported in Asia, where the modern species occur. Herein, 21 fossil leaves identified as Rhodoleia tengchongensis sp. nov. are described from the Upper Pliocene of Tengchong County, Yunnan Province, Southwest China. The fossils exhibit elliptic lamina with entire margins, simple brochidodromous major secondary veins, mixed percurrent intercostal tertiary veins, and looped exterior tertiaries. The leaf cuticle is characterized by pentagonal or hexagonal cells, stellate multicellular trichomes, and paracytic stomata. The combination of leaf architecture and cuticular characteristics suggests that the fossil leaves should be classified into the genus Rhodoleia. The fossil distributions indicate that the genus Rhodoleia might originate from Central Europe, and that migrated to Asia prior to the Late Pliocene. Additionally, insect damage is investigated, and different types of damage, such as hole feeding, margin feeding, surface feeding, and galling, are observed on the thirteen fossil leaves. Based on the damage frequencies for the fossil and extant leaves, the specific feeding behavior of insects on Rhodoleia trees appears to have been established as early as the Late Pliocene. The high occurrence of Rhodoleia insect herbivory may attract the insect-foraging birds, thereby increasing the probability of pollination.  相似文献   

基于双边结构张量的局部自适应图像超分辨率重建     

岳林蔚  沈焕锋  袁强强  张良培  兰霞 《武汉大学学报(信息科学版)》2015,(4):493-497,528

利用超分辨率重建技术可以有效提高图像的空间分辨率,其中先验模型的选取尤为关键。在最大后验概率(MAP)的框架下引入双边结构张量测度,联合像素邻域4个方向的梯度算子提出一种局部自适应先验方法,构建图像超分辨率重建模型,并利用迭代重加权范数(iteratively reweighted norm)对其进行转换求解。基于标准的测试图像进行了实验,并将本方法与拉普拉斯先验、Huber-Markov先验以及BTV先验的重建结果进行对比,验证了该方法的有效性。  相似文献   

Towards More Snow Days in Summer since 2001 at the Great Wall Station,Antarctic Peninsula:The Role of the Amundsen Sea Low     

Minghu DING  Wei HAN  Tong ZHANG  Xiaoyuan YUE  Jeremy FYKE  Ge LIU  Cunde XIAO 《大气科学进展》2020,37(5):494-504

The variation in the precipitation phase in polar regions represents an important indicator of climate change and variability.We studied the precipitation phase at the Great Wall Station and Antarctic Peninsula(AP)region,based on daily precipitation,synoptic records and ERA-Interim data during the austral summers of 1985?2014.Overall,there was no trend in the total precipitation amount or days,but the phase of summer precipitation(rainfall days versus snowfall days)showed opposite trends before and after 2001 at the AP.The total summer rain days/snow days increased/decreased during 1985?2001 and significantly decreased at a rate of?14.13 d(10 yr)?1/increased at a rate of 14.31 d(10 yr)?1 during 2001?2014,agreeing well with corresponding variations in the surface air temperature.Further,we found that the longitudinal location of the Amundsen Sea low(ASL)should account for the change in the precipitation phase since 2001,as it has shown a westward drift after 2001[?41.1°(10 yr)?1],leading to stronger cold southerly winds,colder water vapor flux,and more snow over the AP region during summertime.This study points out a supplementary factor for the climate variation on the AP.  相似文献   

Simulation of the Direct Radiative Effect of Mineral Dust and Sea Salt Aerosols in a Doubled Carbon Dioxide Climate          下载免费PDF全文

YUE Xu  LIAO Hong  TANG Jin-Ping 《大气和海洋科学快报》2013,6(5):343-348

The authors examine the equilibrium climatic response to the direct radiative effect(DRE)of mineral dust and sea salt aerosols in a doubled-CO2climate with two-way coupling of aerosol-climate interactions.In response to the drier and windier conditions,dust emissions increase by 26%in the Sahara Desert and by 18%on the global scale relative to present day.Sea salt emissions increase in high latitudes(60°)but decrease in middle latitudes(30°–60°)of both hemispheres due to the poleward shift of westerlies,leading to a 3%decrease in global emissions.The burdens of dust and sea salt increase by 31%and 7%respectively,because reductions in rainfall over the tropical oceans increase the lifetime of particles in the warmer climate.The higher aerosol loading in the doubled-CO2climate reinforces aerosol DRE by0.2 W m 2,leading to an additional cooling of 0.1°C at the surface compared with the climatic effects of aerosols in present day.The additional cooling from changes in natural aerosols compensates for up to 15%of the regional warming induced by doubled CO2.  相似文献