Development of two novel CYP-antibodies and their use in a PCB exposure experiment with Mytilus edulis     

Jonsson H  Sandnes KV  Schiedek D  Schneider R  Grøsvik BE  Goksøyr A 《Marine environmental research》2004,58(2-5):655-658

In an attempt to learn more about the cytochrome P450 (CYP) system of mussels, we used protein databases and alignment software to extract highly conserved CYP sequences. From these alignments synthetic peptides were produced and used for rabbit immunisation, which yielded polyclonal antibodies against the CYP families 2 and 4. The antibodies were evaluated with Western Blot and ELISA assays, using digestive gland microsomal samples from the mussel Mytilus edulis. Western Blots revealed immunoreactions for both antibodies. The anti-CYP2 sequence rendered one major immunopositive protein of approximately 49 kDa size, and weak signals for proteins of approximately 41 and 56 kDa size. The anti-CYP4 sequence rendered two major bands of approximately 56 and 59 kDa size, and also a weak immunoreaction with a protein of approximately 43 kDa size. ELISA rendered only weak signals even with a 1:50 dilution of IgG-purified serum. A 10-day exposure to Aroclor 1254 did not appear to affect any of the immunopositive proteins, while total PCBs in soft bodies increased from 14-40 ng/g DW in controls to 373-638 ng/g DW in exposed mussels.  相似文献   

烟台海胆体内的纤毛虫和太平洋海胆体内纤毛虫的地理分布     

A.斯特列科夫 《海洋与湖沼》1959,2(1):61-67

1957年夏,中苏海洋动物综合考察队在黄海沿岸烟台地区工作时,参加考察队的阿·阿·斯特列科夫教授采集了海胆肠内的纤毛虫,并以此作为这篇文章的材料。在本文内将提出所见到的纤毛虫名称,并分析它们在太平洋地理分布的情况。  相似文献   

Macrofauna impact on Ulva rigida C. Ag. production and relationship with environmental variables in the lagoon of Venice   总被引：2，自引：0，他引：2  

Balducci C  Sfriso A  Pavoni B 《Marine environmental research》2001,52(1):27-49

The grazing pressure of the macrofaunal invertebrates associated with the biomass of Ulva rigida in the Venice lagoon, their species composition and relationship with environmental variables have been studied. Fifteen sampling campaigns were carried out during different seasons both in the central basin of the lagoon, dominated by macroalgae (especially U. rigida C. Ag.) and in the southern basin, prevalently populated by seagrasses (especially Zostera marina L.). Replicate experiments were conducted in the field by exposing Ulva fronds in net cages of 10 and 1 mm (control) mesh-sizes to allow or prevent grazer entrance. The grazing pressure was determined as Ulva growth rate difference in the cages. In the absence of invertebrate herbivores, Ulva exhibited per cent relative growth rates (%RGRs) ranging from 1.5 to 9.5% day(-1), whereas in their presence the %RGRs were significantly lower (from -2.5 to 3.4% day(-1)) and frequently negative, especially in the station dominated by macroalgae. In this area, peak grazing rates and macrofauna biomasses of up to 8.6% day(-1) and 1,480 g m(-2) fwt (84.4 ash-free dry weight), respectively, were found. On the whole, during in field experiments in the Ulva-dominated station, herbivores removed an amount of biomass whose percentage ranged from 59 to 165% (mean: 103%) of the biomass yield (grazers excluded) found in the cages. These results suggest the possibility that grazers could act as an important factor affecting Ulva production in the Venice lagoon. Macrofauna populations were analysed by means of multivaliate techniques applied to biological variables only and biological and environmental variables together. Data of individual abundance, after a log(x + 1) transformation and the calculation of the Bray-Curtis matrix, were classified using the Cluster Analysis and ordinated by means of the Non-Metric Multidimensional Scaling (MDS) technique, in accordance with the strategies used in the study of multispecies distributions. Finally, biological and environmental variables were analysed together by means of correlation matrices and the Principal Component Analysis.  相似文献   

泛大陆的具有两种机制的两阶段分裂作用     

Hynes  A 朱起煌 《海洋地质译丛》1991,(1):37-41

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洋脊的岩浆房有多大？     

Nicol.  A 陶鲁 《海洋地质译丛》1993,(3):75-78

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Measurement of DNA single-strand breaks in gill and hemolymph cells of mussels, Mytilus sp., collected on the French Atlantic Coast     

Akcha F  Tanguy A  Leday G  Pelluhet L  Budzinski H  Chiffoleau JF 《Marine environmental research》2004,58(2-5):753-756

DNA single-strand breaks were measured by the comet assay in both gill and hemolymph cells of mussels collected in 3 sampling areas of the French coast (Pointe du Castelli, Pen Bron and Saint-Nazaire Harbour). Whole mussel tissue samples were also collected for the chemical determination of PAH, PCB and heavy metal concentrations. In mussel, a higher level of DNA strand breaks was measured in gill than in hemolymph cells (p < 0.01). Despite a factor of contamination from 2 to 3 between sites, no difference in the extent of mussel DNA strand breaks was shown between sampling locations (p > 0.05), questioning the sensitivity of the assays used in biomonitoring studies.  相似文献   

北海盆地第三纪深海扇的定量分析     

T．Reynolds何拥军  刘健 《海洋地质前沿》1995,(3):12-13

北海盆地第三纪深海扇的定量分析Ｔ．Ｒｅｙｎｏｌｄｓ深海扇内富存有大量的碳氢化合物。因此，无论是从扇容积作为石油储层还是在生产期提供水的补给方面来说，它们的规模、几何形态、砂的体积、纯砂体积与总体积之比以及砂的连通性都是石油工业的关键问题。但是，至今深...  相似文献   

海洋微生物学的主要任务     

A.E.科利斯 《海洋与湖沼》1958,1(2):337-343

海——微生物栖息的特殊环境。特殊的盐类成分,低温,高压,低浓度的有机物质,植物及动物区系的稀少等组成了微生物生命活动的广阔海洋区域一些主要生态特征。 海洋中最初的微生物学研究,早在上一世纪就已进行了,但足它们局限于较狭小的任务——  相似文献   

A NEW MODEL FOR PEBBLE BEACH TRACER DISPERSAL     

A. T. WILLIAMS 《海洋学报(英文版)》1987,6(2):229-234

A series of eight tracer experiments were carried out over one year (1983) on a low energy, macro-tidal beach in South Wales, UK. Tracer movement was monitored and 30 pebbles adjacent to coloured tracer pebbles were measured. Measurements were of the A, B, and C axes, roundness, Maximum Projection Sphericity and Oblate-Prolate indices. Analysis shows that through time, the percentage rejection of these properties increased. It is theorised that the surface tracers are 'misfits' or 'rejects' which have not found their equilibrium position in the pebble facies.  相似文献   

西北巴拉望GSEC66.区块深水区石油潜力     

Alessandro  O．Sales  万玲 《海洋地质》2005,(3):6-27

本文论述了Philodfill GSEC66区块，即人们称之为“西北Malampaya区块”的石油潜力评价结果。相信该区块存在主要烃类聚集所必需的要素。可能的烃源岩是深水尼多灰岩，早第三纪同裂谷期前尼多沉积和前第三纪沉积。潜在储集层包括尼多礁隆、尼多和前尼多碎屑岩和，或破碎碳酸盐岩，早中新世Galoc碎屑浊积岩和前尼多沉积。该地区识别出的主要油藏类型是晚渐新世一早中新世礁和侵蚀／喀斯特碳酸岩高地。在对已有的1982年以前地震资料评价后，又采集了1，625km的地震资料。新的地震资料解释后，在这一地区划出了11个预选区，其中2个勘探区（Santa Monica和Ipil勘探区）。Santa Monica是在尼多灰岩上圈出的一个十字倾角圈闭，垂直闭合高度超过457m，闭合面积超过83km^2。Ipil勘探区发育在尼多灰岩台地面上，具缓倾闭合特征，闭合面积超过47km^2，垂直闭合高度122m。这个大型勘探圈出的技术价值与菲律宾十分有利的深水勘探条件及其邻近的即将开发的Malapaya—Camago油气田结合，使这一区块成为今后勘探工作中一个极具前景的地区。  相似文献