Microbial responses using denaturing gradient gel electrophoresis to oil and chemical dispersant in enclosed ecosystems     

Yoshida A  Nomura H  Toyoda K  Nishino T  Seo Y  Yamada M  Nishimura M  Wada M  Okamoto K  Shibata A  Takada H  Kogure K  Ohwada K 《Marine pollution bulletin》2006,52(1):89-95

Microbial responses to the addition of oil with or without a chemical dispersant were examined in mesocosm and microcosm experiments by using denaturing gradient gel electrophoresis of bacterial ribosomal DNA and direct cell counting. When a water-soluble fraction of oil was added to seawater, increases in cell density were observed in the first 24h, followed by a decrease in abundance and a change in bacterial species composition. After addition of an oil-dispersant mixture, increases in cell density and changes in community structure coincided, and the amount of bacteria remained high. These phenomena also occurred in response to addition of only dispersant. Our results suggest that the chemical dispersant may be used as a nutrient source by some bacterial groups and may directly or indirectly prevent the growth of other bacterial groups.  相似文献   

Slab-fluid metasomatism in the Early Paleozoic forearc mantle deduced from the Motai serpentinites,South Kitakami Belt,northeast Japan     

Ayaka Okamoto  Yuji Ichiyama  Akihiro Tamura  Tomoaki Morishita 《Island Arc》2021,30(1):e12387

The present study examines the petrology and geochemistry of the Early Paleozoic Motai serpentinites, the South Kitakami Belt, northeast Japan, to reveal the subduction processes and tectonics in the convergent margin of the Early Paleozoic proto-East Asian continent. Protoliths of the serpentinites are estimated to be harzburgite to dunite based on the observed amounts of bastite (orthopyroxene pseudomorph). Relic chromian spinel Cr# [=Cr/(Cr + Al)] increases with decreasing amount of bastite. The compositional range of chromian spinel is similar to that found in the Mariana forearc serpentinites. This fact suggests that the protoliths of the serpentinites are depleted mantle peridotites developed beneath the forearc regions of a subduction zone. The Motai serpentinites are divided into two types, namely, Types 1 and 2 serpentinites; the former are characterized by fine-grained antigorite and lack of olivine, and the latter have coarse-grained antigorite and inclusion-rich olivine. Ca-amphibole occurs as isolated crystals or vein-like aggregates in the Type 1 serpentinites and as needle-shaped minerals in the Type 2 serpentinites. Ca-amphibole of the Type 1 serpentinites is more enriched in LILEs and LREEs, suggesting the influence of hydrous fluids derived from slabs. By contrast, the mineral assemblage, mineral chemistry, and field distribution of the Type 2 serpentinites reflect the thermal effect of contact metamorphism by Cretaceous granite. The Ca-amphibole of the Type 1 serpentinites is different from that of the Hayachine–Miyamori Ophiolite in terms of origin; the latter was formed by the infiltration of melts produced in an Early Paleozoic arc–backarc system. Chemical characteristics of the Ca-amphibole in the ultramafic rocks in the South Kitakami Belt reflect the tectonics of an Early Paleozoic mantle wedge, and the formation of the Motai metamorphic rocks in the forearc region of the Hayachine–Miyamori subduction zone system, which occurred at the Early Paleozoic proto-East Asian continental margin.  相似文献   

A comparison of neighbourhood relations based on ordinary Delaunay diagrams and area Delaunay diagrams: an application to define the neighbourhood relations of buildings     

Hiroyuki Usui  Akihiro Teraki  Kei-ichi Okunuki  Toshiaki Satoh 《International journal of geographical information science》2020,34(11):2177-2203

ABSTRACT

The aim of this article is to describe a convenient but robust method for defining neighbourhood relations among buildings based on ordinary Delaunay diagrams (ODDs) and area Delaunay diagrams (ADDs). ODDs and ADDs are defined as a set of edges connecting the generators of adjacent ordinary Voronoi cells (points representing centroids of building polygons) and a set of edges connecting two centroids of building polygons, which are the generators of adjacent area Voronoi cells, respectively. Although ADDs are more robust than ODDs, computation time of ODDs is shorter than that of ADDs (the order of their computation time complexity is O(nlogn)). If ODDs can approximate ADDs with a certain degree of accuracy, the former can be used as an alternative. Therefore, we computed the ratio of the number of ADD edges to that of ODD edges overlapping ADDs at building and regional scales. The results indicate that: (1) for approximately 60% of all buildings, ODDs can exactly overlap ADDs with extra ODD edges; (2) at a regional scale, ODDs can overlap approximately 90% of ADDs with 10% extra ODD edges; and (3) focusing on judging errors, although ADDs are more accurate than ODDs, the difference is only approximately 1%.  相似文献   

Crop evapotranspiration in the Nile Delta under different irrigation methods     

Michiaki Sugita  Akihiro Matsuno  Rushdi M. M. El-Kilani  Ahmed Abdel-Fattah  M. A. Mahmoud 《水文科学杂志》2017,62(10):1618-1635

Eddy correlation measurements within the Nile Delta allowed the determination of evapotranspiration (E) for seven crops (rice, maize, cotton, sugar beet, berseem, wheat and fava beans) using basin irrigation (BI), furrow irrigation (FI), BI with increased intervals (BI_i), FI with increased intervals (FI_i), strip irrigation (SI) and drip irrigation (DI). Total E values over the cropping season for rice (BI, BI_i) were the highest (>600 mm), while those for sugar beet (DI), maize (SI and DI) and berseem (BI_i) were the lowest (<250 mm). The differences were due to a combination of atmospheric demand, soil moisture, the presence of surface standing water, root depth, and the length and timing of the cropping season. The DI and SI methods had the advantage for water saving, while the FI_i and BI_i methods were effective for crops with shallow root lengths. Estimated annual E was 566–828 mm/year (water-saving irrigation) and 875–1225 mm/year (conventional irrigation).  相似文献   

库木库里背斜活动性初步研究          下载免费PDF全文

孙浩越  何宏林  付碧宏  杨顺虎  白濱吉起  池田安隆  狩野谦一  越後智雄 《地震地质》2014,36(1):1-13

库木库里盆地位于青藏高原北缘,与柴达木盆地一山之隔,是二者的过渡地带,也是高原主体部分向NE扩展的前缘地区;现今构造表现为被3条大型活动构造带(走滑的阿尔金断裂带、东昆仑断裂带和逆冲的祁漫塔格褶皱逆冲系)所夹持。因此,该盆地对于研究青藏高原北缘的构造活动性、活动历史,探讨高原的扩展模式具有十分重要的意义。虽然库木库里盆地南、北两侧均发育活动性很强的大型走滑断裂,但是在盆地中央发育1条大型背斜,走向NWW-SEE,与祁漫塔格褶皱逆冲系和柴达木盆地内的褶皱构造走向一致,说明盆地目前遭受NNE向的挤压。通过对盆地地形横、纵剖面和阶地展布形态的分析,得出背斜有自西向东扩展变形的特征;野外调查和测年结果显示,背斜东段冰川融水形成了大型冰水扇,形成年龄为(87.09±2.31)~(102.4±3.7)ka,进而获得背斜东段自晚更新世以来平均隆升速率的最大值为(2.78±0.28)~(3.28±0.28)mm/a。库木库里盆地整体的活动性很强,在构造上与其北边的柴达木盆地类似,都受控于阿尔金断裂南侧的NNE向的区域挤压作用。  相似文献   

Raman spectroscopic and calorimetric observations on natural gas hydrates with cubic structures I and II obtained from Lake Baikal     

Akihiro Hachikubo  Oleg Khlystov  Masato Kida  Hirotoshi Sakagami  Hirotsugu Minami  Satoshi Yamashita  Nobuo Takahashi  Hitoshi Shoji  Gennadiy Kalmychkov  Jeffrey Poort 《Geo-Marine Letters》2012,32(5-6):419-426

This study reports measurements of the Raman spectra of Lake Baikal gas hydrates and estimations of the hydration number of methane-rich samples. The hydration number of gas hydrates retrieved from the southern Baikal Basin (crystallographic structure I) was approx. 6.1. Consistent with previous results, the Raman spectra of gas hydrates retrieved from the Kukuy K-2 mud volcano in the central Baikal Basin indicated the existence of crystallographic structures I and II. Measurements of the dissociation heat of Lake Baikal gas hydrates by calorimetry (from the decomposition of gas hydrates to gas and water), employing the hydration number, revealed values of 53.7–55.5?kJ?mol^–1 for the southern basin samples (structure I), and of 54.3–55.5?kJ?mol^–1 for the structure I hydrates and 62.8–64.2?kJ?mol^–1 for the structure II hydrates from the Kukuy K-2 mud volcano.  相似文献   

Thermal anomalies associated with shallow gas hydrates in the K-2 mud volcano, Lake Baikal     

Jeffrey Poort  Oleg M. Khlystov  Lieven Naudts  Albert D. Duchkov  Hitoshi Shoji  Shin’ya Nishio  Marc De Batist  Akihiro Hachikubo  Masato Kida  Hirotsugu Minami  Andrey Y. Manakov  Marina V. Kulikova  Alexey A. Krylov 《Geo-Marine Letters》2012,32(5-6):407-417

Thermal measurements and hydrate mapping in the vicinity of the K-2 mud volcano in Lake Baikal have revealed a particular type of association of thermal anomalies (29–121?mW?m^–2) near hydrate-forming layers. Detailed coring within K-2 showed that hydrates are restricted to two distinct zones at sub-bottom depths exceeding 70–300?cm. Temperature data from stations with hydrate recovery and degassing features all display low thermal gradients. Otherwise, the thermal gradients within the mud volcano are generally increased. These findings imply a more complicated thermal regime than often assumed for mud volcanoes, with important roles for both fluids and hydrates. The coexistence of neighbouring low and high thermal anomalies is interpreted to result from discharging and recharging fluid activity, rather than hydrate thermodynamics. It is suggested that hydrates play a key role in controlling the fluid circulation pattern at an early stage. At a later stage, the inflow of undersaturated lake water would favour the dissolution of structure I hydrates and the formation of structure II hydrates, the latter having been observed on top of structure I hydrates in the K-2 mud volcano.  相似文献   

Evidence of the restricted gene flow within a small spatial scale in the Japanese common intertidal goby Chaenogobius annularis     

Shotaro Hirase  Manami Kanno  Minoru Ikeda  Akihiro Kijima 《Marine Ecology》2012,33(4):481-489

Elucidating the scale of gene flow among populations is an important challenge for understanding the ecological dynamics and local adaptation of marine organisms. We assessed whether gene flow is restricted even at a small spatial scale in the Japanese common intertidal goby Chaenogobius annularis, using highly polymorphic DNA markers, involving the mitochondrial DNA (mtDNA) control region and 15 microsatellite DNA (msDNA), because past ecological studies have suggested low dispersal ability for rocky intertidal fishes. We found significant heterogeneities between four neighboring local populations by both mtDNA and msDNA analyses. In addition, no genetic heterogeneity was detected by either method across generations within a population; it was considered that such genetic differentiation is retained across generations and that the gene flow of this species is restricted to within a radius of a few kilometers. This is the first report showing a clear genetic subdivision in rocky intertidal fish.  相似文献   

Evolution of animal multicellularity stimulated by dissolved organic carbon in early Ediacaran ocean: DOXAM hypothesis     

AKIHIRO KANO  YOKO KUNIMITSU  TETSUHIRO TOGO  CHIDURU TAKASHIMA  FUMITO SHIRAISHI  WEI WANG 《Island Arc》2011,20(2):280-293

Oxygenation of the ocean is presumed to be an important factor stimulating the evolution of multicellular animals. The appearance of the Ediacaran‐type biota (ca 575 Ma) was assigned to the aftermath of the Gaskiers glaciation (ca 580 Ma), when substantial oceanic oxygenation is believed to have started. However, several lines of evidence reveal that at least sponges evolved before this oxygenation. For understanding the first stage of animal evolution, we propose the hypothesis that Dissolved Organic Carbon (DOC) Stimulated the evolution for Animal Multicellularity (DOXAM). Recent geochemical studies of the Ediacaran sedimentary sequences have indicated that a substantial DOC mass was developed in the stratified ocean after the Marinoan glaciation (655–635 Ma), and this was supported by the inorganic and organic carbon isotope profiles of the Doushantuo Formation in South China. The DOC mass was an oxygen consumer in the water column; however, it could have provided a food source for filter‐feeding animals such as sponges and cnidarians, and established a primitive food‐web. Such an ecological structure is recognized in modern deep‐sea coral mounds. Results from the integrated ocean drilling program (IODP) Expedition 307 for a mound in northeastern Atlantic suggested that organic carbon suspended around the density boundary in the water column is the key feature to feed the heterotrophic deep‐sea coral community. Our hypothesis is consistent with the fact that the two most primitive animal phyla (Porifera and Cnidaria) are filter feeders. The evolution of filter feeding ecosystems removed the DOC mass and may have contributed to ocean oxygenation in the terminal Neoproterozoic when animal evolution passed into the second stage, with the appearance of bilaterians.  相似文献   

The succession of microbial community in the organic rich fish-farm sediment during bioremediation by introducing artificially mass-cultured colonies of a small polychaete, Capitella sp. I     

Kunihiro T  Miyazaki T  Uramoto Y  Kinoshita K  Inoue A  Tamaki S  Hama D  Tsutsumi H  Ohwada K 《Marine pollution bulletin》2008,57(1-5):68-77

We monitored seasonal changes of the abundance and composition of microorganisms in the fish-farm sediment in Kusuura Bay, Amakusa, Japan, using the quinone profiling technique, during bioremediation by introducing cultured colonies of polychaete, Capitella sp. I. In November 2004, approximately 9.2 million cultured worms were transferred to the fish-farm sediment, which increased rapidly, and reached 458.5 gWW/m(2) (528,000 indiv./m(2)) in March 2005. During this fast-increasing period of Capitella, the microbial quinone content of the surface sediment (0-2 cm) also increased markedly, and reached 237 micromol/m(2) in January 2005, although the water temperature decreased to the lowest levels in the year. Particularly, the mole fraction of ubiquinone-10 in total quinones in the sediment, indicating the presence of alpha subclass of Proteobacteria, increased by 9.3%. These facts suggest that the bacterial growth was enhanced markedly by the biological activities of worms in the sediment, and the bacteria played an important role in the decomposition of the organic matter in the sediment.  相似文献