凡纳滨对虾咸淡水养殖系统内细菌群落组成的PCR-DGGE分析   总被引：16，自引：0，他引：16  

罗鹏  胡超群  谢珍玉  张吕平  任春华  许尤厚 《热带海洋学报》2006,25(2):49-53

采用PCR(polym erase chain reaction)-DGGE(denaturing grad ient gel electrophoresis)及传统的微生物培养方法对凡纳滨对虾Litopenaeus vannam ei咸淡水养殖系统内各种环境的细菌群落组成进行了比较研究。传统的微生物培养计数表明,从海湾水、养殖池水到对虾粪样,细菌和弧菌的数量表现出依次增加的趋势,粪样及肠壁中弧菌的数量高出外界水环境1—4个数量级。相对于外界水环境,粪样中有很高的芽孢杆菌孢子含量,但是肠壁定植细菌中不存在芽孢杆菌(孢子)。PCR-DGGE及聚类分析结果表明,从海湾沿岸、养殖池、对虾粪便到对虾肠壁,细菌群落的多样性由高到低,无论是在哪种环境,群落的优势种都十分明显,且只有2—4种。来自同一环境各样品间的细菌群落组成非常相似,来自不同环境的样品,其细菌群落组成差别较大。聚类图上各簇的排列顺序反映了样品在取样空间分布上的毗邻次序和它们的相似程度。  相似文献   

Paleomonsoon precipitation deduced from a sediment core from the equatorial Indian Ocean     

M. Tiwari  R. Ramesh  B. L. K. Somayajulu  A. J. T. Jull  G. S. Burr 《Geo-Marine Letters》2006,26(1):23-30

Rapid shifts in past climate recorded in polar ice sheets have elicited various explanations relating to either thermohaline circulation changes by ice-rafting or natural greenhouse gas concentrations modulated by climatic conditions in the tropics. To compare the tropical paleoclimate record with the polar record, one must choose sediment cores from highly productive ocean regions. Necessarily, such regions reflect the wind records in the tropics, because high productivity is associated with upwelling driven by winds. Comparing tropical precipitation records with high-latitude records is, however, a more difficult task because sediments recording paleoprecipitation usually have low sedimentation rates, and offer coarser resolution relative to polar ice cores. Here, we present δ ¹⁸O data of three planktonic species of Foraminifera (a proxy for precipitation) from such a sediment core, spanning the past 35 ka for the equatorial Indian Ocean, which falls under the southwest monsoon (SWM) realm. Results show that minimum SWM precipitation occurred at the Last Glacial Maximum, with a subsequent increase at Termination IA. During the Holocene, SWM precipitation intensified uniformly up to the core top (∼2.2 ka b.p.), as revealed by generally decreasing δ ¹⁸O values. Variations in precipitation are consistent with climate changes recorded in polar ice sheets. Although the different resolutions of the two records preclude a rigorous comparison, abrupt cooling/warming events appear to be accompanied by sudden reduction/enhancement in (SWM) rainfall. Thus, mechanisms with time scales much shorter than a millennium, such as natural greenhouse warming (e.g., CH₄ concentration), controlled by emissions from the tropics, could have played a major role in high-latitude climate change.  相似文献   

Fish-farming effects on benthic community structure in coastal sediments: analysis of meiofaunal recovery   总被引：8，自引：0，他引：8  

Mazzola  A.; Mirto  S.; La Rosa  T.; Fabiano  M.; Danovaro  R. 《ICES Journal of Marine Science》2000,57(5):1454-1461

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Shipborne GPS attitude determination during MMST-93   总被引：1，自引：0，他引：1  

Lachapelle  G. Cannon  M.E. Lu  G. Loncarevic  B. 《Oceanic Engineering, IEEE Journal of》1996,21(1):100-104

The attitude parameters of a ship underway were measured using a configuration of four 10-channel NovAtel Model 951 narrow-correlator-spacing receivers. These C/A code receivers have output rates of up to 10 Hz and maintain effective carrier phase lock under relatively harsh ship dynamics. The attitude parameters are calculated independently at each epoch using differential carrier phase measurements, carrier phase ambiguities are resolved on-the-fly by constraining the approximately known distances between the antennas that are rigidly mounted on the ship. Carrier phase thermal noise and multipath are minimized by mounting the antennas as far apart as possible. The four-antenna configuration provides redundancy and further improves accuracy. During the Matthew Motion Sensor Trials (MMST-93) conducted off the coast of Halifax, Nova Scotia, in June-July 1993, the GPS-derived attitude parameters were compared with those obtained with a Honeywell HG1050 ring laser gyro inertial navigation system (INS) which provides roll and pitch with an accuracy of 15 arcsecs and heading with an accuracy of 2 arcmins. To simulate rough weather conditions, sharp maneuvers were performed to induce roll angles in excess of 10°. No accuracy degradation nor any loss of GPS measurements occurred. The RMS agreement between GPS and INS derived attitude parameters is better than 2 arcmins in heading, 1 arcmin in pitch and 3 arcmins in roll. This level of accuracy demonstrates the capability of GPS for cost-effective shipborne attitude determination at an accuracy level of 0.05  相似文献   

两种涡鞭毛藻的周日垂直迁移特性研究   总被引：3，自引：3，他引：3  

周名江  M. Elbrachter 《海洋与湖沼》1994,25(2):173-178

Ａｌｅｘａｎｄｒｉｕｍ ｌｕｓｉｔａｎｉｃｕｍ于１９８７年采自葡萄牙沿海，Ｙ－１００于１９８９年采自德国湾。在暗室中用一根柱长１５０ｃｍ，内径３．４ｃｍ的玻璃柱对两种涡鞭毛藻的垂直行特性进行研究。结果表明，两种藻均进行有规律的周日垂直迁移，且均在始前２ｈ开始向上迁移，光照结束前３ｈ开始向移速度约为２８０μｍ／ｓ，向下约为１４０μｍ／ｓ向下约为８５０μｍ／ｓ；Ｙ－１００向上迁移速度约为２８０μｍ／ｓ  相似文献   

Artificial Reefs in the Low Productive Marine Environments of the Southeastern Mediterranean   总被引：1，自引：0，他引：1  

E. Spanier    M. Tom    S. Pisanty  G. Almog-Shtayer   《Marine Ecology》1990,11(1):61-75

Abstract. Fishery yields in the oligotrophic waters of the Southeastern Mediterranean arc low due to habitat and food limitations. A four year study of a complex of artificial structures established in the coastal waters of Israel points to several possible solutions to these problems. One approach is the artificial enrichment of the man-made reef with trash fish. This, together with the configuration of the reef, caused a significant increase in the abundance of fish predators such as groupers of the genus Epinephclus. Omnivores and herbivores were attracted only by the presence of artificial structures. Some species, such as the slipper lobster Scyllurides lulus, only use the man-made reef for shelter during part of the day or season; they forage over a much larger area the rest of the time and are important "importers" of food to the artificial habitat. Artificial kelp were effective in recruiting juveniles and small fishes to the site.  相似文献   

Nankai Trough: A hot trench?     

M. Yamano  S. Honda  S. Uyeda 《Marine Geophysical Researches》1984,6(2):187-203

Heat flow estimated from the gas hydrate layers on the landward slope of the Nankai Trough reveals that heat flow increases downslope toward the trench floor. This data plus six new heat flow values obtained by a conventional probe and two values available from DSDP drill holes give a fairly detailed heat flow distribution in the Nankai Trough area, when combined with the already existing data set. There appears to be a zonal pattern parallel to the trough axis, with a high heat flow zone on the floor of the trough that is quite anomalous for a subduction zone. It might be explained as a result of subduction of the hot portion of the Philippine Sea plate, i.e. the Shikoku Basin, and/or of more local effects such as heating due to intrusion of hot water from subducted sediments to shallow depth beneath the trough floor. Surface heat flow patterns landward of the trough were calculated for a simple thermal model of subduction. Perfect reproduction of the observed zonal pattern is difficult to achieve by the simple model, suggesting the necessity for further heat flow and other observations.  相似文献   

Otolith shape as a stock discrimination tool for ling (Genypterus blacodes) in the fjords of Chilean Patagonia     

Rodrigo Wiff  Andrés Flores  Angel M. Segura  Mauricio A. Barrientos  Vilma Ojeda 《新西兰海洋与淡水研究杂志》2020,54(2):218-232

ABSTRACT

Genypterus blacodes, in terms of its fishing history and local economic importance, is an emblematic species harvested in Chilean Patagonia (41°00’–57°00’S). Most of the current fisheries and biological knowledge of this species come from the open ocean, whereas information about the species in fjords and inner channels is fragmentary. In 2018, two research surveys targeting G. blacodes were conducted in the fjords and inner channels of Chilean Patagonia. A total of 253 pairs of sagittal otoliths were sampled at three different localities, and their contours were modelled using wavelet analysis as a tool for stock discrimination. Contours were compared using canonical analysis, and classification was performed using linear discriminant and Random Forest analyses. The results indicated that the wavelet method is efficient in modelling otolith contours, and the discriminant analyses showed differences among fishing grounds across the latitudinal gradient, thus confirming the hypothesis that G. blacodes conform to at least two separate stock units in Chilean Patagonia. Fishing grounds that were closer in space showed higher levels of misclassification. The discussion focuses on how environmental variables and the geography of fjords shape stock differences and how this information can be used for the sustainable management of G. blacodes.  相似文献   

Colonization, succession, and nutrition of macrobenthic assemblages in a restored wetland at Tijuana Estuary, California     

Serena M. Moseman  Lisa A. Levin  Carolyn Currin  Charlotte Forder   《Estuarine, Coastal and Shelf Science》2004,60(4):755-770

Modes of colonization, the successional trajectory, and trophic recovery of a macrofaunal community were analyzed over 19 months in the Friendship marsh, a 20-acre restored wetland in Tijuana Estuary, California. Traditional techniques for quantifying macrofaunal communities were combined with emerging stable isotopic approaches for evaluation of trophic recovery, making comparisons with a nearby natural Spartina foliosa habitat. Life history-based predictions successfully identified major colonization modes, although most taxa employed a variety of tactics for colonizing the restored marsh. The presence of S. foliosa did not seem to affect macrofaunal colonization or succession at the scale of this study. However, soil organic matter content in the restored marsh was positively correlated with insect densities, and high initial salinities may have limited the success of early colonists. Total macrofaunal densities recovered to natural marsh levels after 14 months and diversity, measured as species richness and the Shannon index (H′), was comparable to the natural marsh by 19 months. Some compositional disparities between the natural and created communities persisted after 19 months, including lower percentages of surface-feeding polychaetes (Polydora spp.) and higher percentages of dipteran insects and turbellarians in the Friendship marsh. As surficial structural similarity of infaunal communities between the Friendship and natural habitat was achieved, isotopic analyses revealed a simultaneous trajectory towards recovery of trophic structure. Enriched δ¹³C signatures of benthic microalgae and infauna, observed in the restored marsh shortly after establishment compared to natural Spartina habitat, recovered after 19 months. However, the depletion in δ¹⁵N signatures of macrofauna in the Friendship marsh indicated consumption of microalgae, particularly nitrogen-fixing cyanobacteria, while macroalgae and Spartina made a larger contribution to macrofaunal diets in the natural habitat. Future successional studies must continue to develop and employ novel combinations of techniques for evaluating structural and functional recovery of disturbed and created habitats.  相似文献   

Growth of polar cod, Boreogadus saida (Lepechin), in the Barents Sea     

Gjosaeter  Harald; Ajiad  Adnan M. 《ICES Journal of Marine Science》1994,51(1):115-120

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