DNA barcode assessment of Ceramiales (Rhodophyta) in the intertidal zone of the northwestern Yellow Sea   总被引：1，自引：0，他引：1  

杜国英  吴菲菲  郭皓  薛红凡  茅云翔 《中国海洋湖沼学报》2015,33(3):685-695

A total of 142 specimens of Ceramiales(Rhodophyta) were collected each month from October 2011 to November 2012 in the intertidal zone of the northwestern Yellow Sea. These specimens covered 21 species,14 genera,and four families. Cluster analyses show that the specimens had a high diversity for the three DNA markers,namely,partial large subunit r RNA gene(LSU),universal plastid amplicon(UPA),and partial mitochondrial cytochrome c oxidase subunit I gene(COI). No intraspecific divergence was found in our collection for these markers,except for a 1–3 bp divergence in the COI of Ceramium kondoi,Symphyocladia latiuscula,and Neosiphonia japonica. Because short DNA markers were used,the phylogenetic relationships of higher taxonomic levels were hard to evaluate with poor branch support. More than half species of our collection failed to find their matched sequences owing to shortage information of DNA barcodes for macroalgae in Gen Bank or BOLD(Barcode of Life Data) Systems. Three specimens were presumed as H eterosiphonia crispella by cluster analyses on DNA barcodes assisted by morphological identification,which was the first record in the investigated area,implying that it might be a cryptic or invasive species in the coastal area of northwestern Yellow Sea. In the neighbor-joining trees of all three DNA markers,H eterosiphonia japonica converged with D asya spp. and was distant from the other Heterosiphonia spp.,implying that H. japonica had affinities to the genus Dasya. The LSU and UPA markers amplified and sequenced easier than the COI marker across the Ceramiales species,but the COI had a higher ability to discriminate between species.  相似文献   

Extensive genetic divergence among Diptychus maculatus populations in northwest China     

孟玮  杨天燕  海萨  马燕武  蔡林钢  马徐发  高天翔  郭焱 《中国海洋湖沼学报》2015,33(3):577-584

D. maculates is a kind of specialized Schizothoracinae fish has been locally listed as a protected animal in Xinjiang Province,China. Ili River located in north of Tianshan Mountain and Tarim River located in north of Qinghai-Tibetan Plateau were two main distribution areas of this fish. To investigate the genetic diversity and genetic structure of D. maculates,four populations from Tarim River system and two populations from Ili River system were collected in this study. A 570-bp sequence of the control region was obtained for 105 specimens. Twenty-four haplotypes were detected from six populations,only Kunes River population and Kashi River population shared haplotypes with each other. For all the populations examined,the haplotype diversity(h) was 0.904 8±0.012 6,nucleotide diversity(π) was 0.027 9±0.013 9,and the average number of pairwise nucleotide differences(k) was 15.878 3±7.139 1. The analysis of molecular variance(AMOVA) showed that 86.31% of the total genetic variation was apportioned among populations,and the variation within sampled populations was 13.69%. Genetic differences among sampled populations were highly significant. F st statistical test indicated that all populations were significantly divergent from each other(P 0.01). The largest F st value was between Yurungkash River population and Muzat River population,while the smallest F st value was between Kunes River population and Kashi River population. NJ phylogenetic tree of D-loop haplotypes revealed two main clades. The neutrality test and mismatch distribution analysis suggested that the fish had went through a recent population expansion. The uplift of Tianshan Mountain and movement of Qinghai-Tibetan Plateau might contribute to the wide genetic divergence of D. maculates in northwest China.  相似文献   

A theoretical framework and methodology for urban activity spatial structure in e-society: Empirical evidence for Nanjing City,China   总被引：7，自引：0，他引：7  

Bo Wang  Feng Zhen  Zongcai Wei  Shu Guo  Tingting Chen 《中国地理科学(英文版)》2015,25(6):672-683

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目标样本粒度判据在海湾环境演变研究中的运用——以福建前湖湾为例     

俞鸣同  刘秀铭  杨华玮  曹宏伟  唐荣骏  刘巍  龚正权  陈有喜  陈樱  孙巧红  郭杰 《海洋学报》2015,37(9):106-112

高密度采集福建前湖湾海岸剖面133个泥沙样品做粒度分析,其频率曲线显示多种粒度分布特征,预示多种沉积环境的变化。采集研究剖面周边海滩、河口浅滩、河口、滨海沼泽和海岸沙丘等已知环境的样品作为目标样本。将目标样本沉积参数平均粒径(Mz)、标准偏差(σ)、峰态(Kg)设为判别参数,利用Excel计算海岸剖面样品与目标样本参数的距离,筛选最小距离样品,归为已知目标样本的同类。据此,分析了海岸剖面蕴含的6个沉积环境变化阶段。利用剖面底部淤泥测年14C=(328 15±170)a BP,和剖面上部泥炭测年14C=(24 130±100)a BP,了解这一变化发生的年代。自(32 815±170)a BP以来,前湖湾经历了河口浅滩-河口、沙丘-河口-滨海沼泽-海滩-沙丘等海岸环境演变过程。  相似文献   

长江口沙波统计特征及输移规律   总被引：4，自引：1，他引：3  

郭兴杰  程和琴  莫若瑜  杨忠勇 《海洋学报》2015,37(5):148-158

利用多波束测深系统对长江口南港、北槽、横沙通道和北港水下地貌进行测量,对沙波波高、波长、迎流倾角、背流倾角、水深和沉积物特征进行统计,并根据流速、沉积物粒径及水深估算其潮周期内净位移。结果表明:长江口沙波基本都为大型沙波,且沙波大小与其所在区域沉积物粒径呈正比关系;长江口各个区域沙波的对称性不同,涨、落潮优势流越明显,则沙波对称性越差,净位移越大;沙波对称性能反映此区域水动力强弱及潮周期内沙波净位移大小。  相似文献   

A long-term volume transport time series estimated by combining in situ observation and satellite altimeter data in the northern South China Sea   总被引：1，自引：0，他引：1  

Xiao-Hua Zhu  Ruixiang Zhao  Xinyu Guo  Yu Long  Yun-Long Ma  Xiaopeng Fan 《Journal of Oceanography》2015,71(6):663-673

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Experimental research on horizontal bearing behavior of caisson-pile composite foundationEI北大核心CSCD     

Wang Lei  Guo Chao  Mu Bao-gang  Gong Wei-ming  Sun Zhen-wei 《岩土力学》2015,(11):3150-+

In this study, four groups of different types of bridge foundation model are tested to research the horizontal bearing behavior of caisson-pile composite foundation in lab based on the Qiongzhou Strait bridge project. The Q-s curve and horizontal ultimate bearing capacity of these four groups of foundations in sandy soil layer are obtained. Pile bending moment and shear force of pile shaft are analyzed in detail. At the same time, the load sharing ratio of caisson and piles is discussed. The results show that the horizontal ultimate bearing capacities of a single caisson foundation are increased by 1.2 times, 1.6 times and 2 times respectively with adding skirts, steel pipe pile, or steel pipe pile and the skirt. The maximum bending moment point is in the middle of the pile shaft, i.e. at about 0.5 m under the mud surface. The horizontal load is borne mainly by the upper caisson foundation and soil layers above the middle upper part of pile. The maximum shear force is found at the joint of pile top of steel pipe and caisson pile, where the reinforcement measures should be taken in practical engineering. The research achievements could provide a better reference for design or construction of caisson-pile composite foundation.  相似文献   

An experimental study on major element release from the sediments in the Changjiang (Yangtze River) Estuary     

Guo  Yanwei  Yang  Shouye 《中国海洋大学学报(英文版)》2015,14(3):417-424

With the enhanced warming and acidification of global ocean, whether and to what extent the naturally-weathered fluvial sediment into the sea can release elements and thus influence the geochemical pro...  相似文献   

2021年5月云南漾濞MS 6.4地震序列发震构造及破裂过程初探     

孙庆山  张伟恒  郭忻怡  王亮 《地质学报》2023,97(2):349-363

北京时间2021年5月21日21时48分36秒,云南省大理州漾濞县发生M_S 6.4地震。利用云南数字地震台网2021年5月18日至8月22日的震相报告,采用双差地震定位法,对漾濞M_S 6.4地震序列进行重新定位。重新定位结果显示序列呈NW向优势分布,破裂长约20 km,宽约7 km,对重新定位结果进行误差分析,水平方向定位误差约为0.8 km,垂直方向定位误差约为1.0 km,定位结果具有较好的稳定性。依据震中分布的走向将序列划分为NW向的主断层与NNW向的分支断层,主断层存在较为明显的分段现象,分支断层呈雁列状分布。根据小震丛集性发生在大震断层面及其附近的原则,利用重新定位后的小震震源位置反演得到漾濞M_S 6.4序列主断层走向约320°,倾角约89°,深度范围3～13 km。根据拟合得到的断层在地表的投影位置,推测本次地震的发震断层为维西-乔后断裂西侧的草坪断裂。基于断层滑动量分布识别出3个凹凸体,结合序列时空演化特征,分析了漾濞M_S 6.4地震序列的破裂过程,结果显示断层中段的凹凸体发生初始破裂...  相似文献   

深度学习支持下的丘陵山区耕地高分辨率遥感信息分区分层提取方法     

刘巍  吴志峰  骆剑承  孙营伟  吴田军  周楠  胡晓东  王玲玉  周忠发 《测绘学报》2021,50(1):105-116

耕地是丘陵山区稀缺的土地资源,具有地形条件复杂、种植结构多样的特点,导致了山地耕地信息难以快速、准确获取,并且基于传统的遥感数据及遥感监测方法开展山区耕地信息快速自动提取比较困难。针对这一问题,本文以西南山区贵州省息烽县作为试验区,根据地理空间异质性特征,提出分区控制、分层提取的耕地形态信息提取思路,构建了一种地貌单元约束条件下的分区分层耕地形态信息的提取方法。该方法首先根据地貌-植被特征将试验区划分为平坝区、山坡区、林草区3类地理分区;然后在每类分区基础上,根据耕地所呈现的视觉特征划分为不同的类型,对不同类型的耕地分别设计不同的深度学习模型进行分层提取。试验结果证明,该方法对山区复杂地形背景噪声具有较好的抑制作用,所提取的耕地地块信息相比于传统方法更符合实际耕地的实际分布形态,有效地减少了漏提率和错提率。  相似文献