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Effect of Bacillus mucilaginosus on weathering of phosphorite and a preliminary analysis of bacterial proteins 总被引:3,自引:0,他引:3
The authors investigated the effect of Bacillus mucilaginosus on weathering of phosphorite. Analysis of different proteins was of significance in exploring the molecular biological mechanism in the bacterial weathering process. The concrete methods are described as follows: Mineral powder was put into liquid culture medium and B. mucilaginosus was incubated in the medium. The control (group) had no mineral powder in the medium. The treatments and controls were cultured simultaneously under the same condition. In a few days, the supernatant was filtrated, the main cations (Ca^2+, Mg^2+, Na^+, Mn^2+, Al^3+, Fe^3+, K^+) were measured by ICP-OES, and the contents of water soluble phosphorus (Pws) and silicon (Siws) were determined by colorimetry. The residual solid was weighed on the filter paper, followed by digestion with concentrated HNO3. The concentrations of the main cations and Pws, Siws in the digest liquid were measured by using the method mentioned above. After the supernatant was centrifuged, the precipitation was used to analyze the protein differences between the treatment groups and the control groups by 2-dimentional gel electrophoresis (2-DE). The experimental results showed that apatite and quartz were partially weathered, but kaolinite was dissolved completely. The population of bacteria increased when mineral powder was added in the liquid medium. Software analysis and comparison of the 2-DE pictures of bacterial proteins revealed 1134 visible protein spots in the treatment group, and 729 visible protein spots in the control group. To compare the bacterial protein expression contents of the treatment group with those of the control group, there were 496 different protein spots, including 214 protein spots which indicated that the protein contents increased, 75 protein spots were indicative of a decrease, and 207 proteins were newly synthesized. It is proposed that the increased bacterial contents may be related to some protein expression and activation after the 相似文献
83.
Benthic nutrient fluxes in the intertidal flat within the Changjiang (Yangtze River) Estuary 总被引:1,自引:0,他引:1
GAO Lei LI Daoji WANG Yanming YU Lihua KONG Dingjiang LI Mei LI Yun and FANG Tao State Key Laboratory of Marine Geology Tongji University Shanghai China State Key Laboratory of Estuarine Coastal Research East China Normal University Shanghai China 《中国地球化学学报》2008,27(1):58-71
In an annual cycle from March 2005 to February 2006, benthic nutrient fluxes were measured monthly in the Dongtan intertidal flat within the Changjiang (Yangtze River) Estuary. Except for NH4^+, there always showed high fluxes from overlying water into sediment for other four nutrients. Sediments in the high and middle marshes, covered with halophyte and consisting of macrofauna, demonstrated more capabilities of assimilating nutrients from overlying water than the low marsh. Sampling seasons and nutrient concentrations in the overlying water could both exert significant effects on these fluxes. Additionally, according to the model provided by previous study, denitrification rates, that utilizing NO3- transported from overlying water (Dw) in Dongtan sediments, were estimated to be from -16 to 193 μmol·h^-1·m^-2 with an average value of 63 μmol·h^-1·m^-2 (n=18). These estimated values are still underestimates of the in-situ rates owing to the lack of consideration of DN, i.e., denitrification supported by the local NO3^- production via nitrification. 相似文献
84.
简要分析了非量测数码相机哈苏H1D在南极中山站及拉斯曼丘陵地区所获取的影像数据的几何精度,阐明了利用该影像数据进行空中三角测量,DLG,DOM制作的技术流程及作业过程中出现问题的处理方法。 相似文献
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中元古代晚期浅海高能沉积环境中的海绿石:以天津蓟县剖面铁岭组为例 总被引:7,自引:0,他引:7
海绿石是一种富钾、富铁的含水层状铝硅酸盐矿物,在沉积学领域常被作为一种普遍的指相矿物。多年研究的结果表明,现代海绿石主要形成在慢速、弱还原的较深水环境中,而且还可以作为"凝缩段"的识别标志之一。天津蓟县剖面中元古界铁岭组第二段灰岩中的海绿石,产在高能叠层石岩礁之中,主要以胶体形式富集在叠层石和均一石的边界上,代表较为典型的原地海绿石;较高的氧化钾含量(大于8%)而显示出高成熟海绿石的特点。很明显,铁岭组二段灰岩中的原地高成熟海绿石,不但不能作为"凝缩段"的识别标志,而且也不是长时间地层间断的产物。由于形成在正常高能浅海环境,而且处于中元古代末期,与现代沉积中的海绿石存在较大的差异,可能代表了中元古代末期的正常浅海还处于含氧量不够充分的弱还原状态,最终使铁岭组灰岩中的海绿石成为前寒武纪海绿石产出的一个典型代表,也间接的表明了在漫长的地质历史演变过程中海绿石产出的多样性特点。 相似文献
88.
塔里木盆地孔雀河地区复合含油气系统与有利勘探方向 总被引:1,自引:0,他引:1
运用叠合盆地复合含油气系统的描述方法和评价思路,分析了塔里木盆地孔雀河地区含油气系统复合特征,再现从源岩到圈闭的油气地质演化过程。孔雀河地区含油气系统以断裂复合贯通为主,同时存在不整合面复合贯通,构成寒武系C—(!)—下奥陶统O1(!)—石炭系C(*)—三叠系T(*)—侏罗系J(*)+寒武系—C(!)—下奥陶统O1(*)—志留系S(*)—泥盆系D(*)—侏罗系J(*)改造型复合含油气系统(*)。含油气系统的复合经历3个关键时刻:泥盆纪末是构造格局与古油藏形成期,侏罗纪末和白垩纪末是油气转化、重新分配与油藏调整期。研究区可分为破坏散失区、改造调整区、深埋保存区,其中改造调整区、深埋保存区为有利油气聚集区,处在改造调整区的龙口背斜和维马克—开屏背斜的上古生界和中生界的断背斜圈闭以及下古生界残留古断背斜圈闭是最佳勘探目标。 相似文献
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Cambrian explosion: Birth of tree of animals 总被引:5,自引:5,他引:0
Excluding the sponges the Kingdom Animalia is usually divided into three subkingdoms: Diploblasta, Protostomia and Deuterostomia. The Cambrian Explosion consists of three major episodes, two of which were in the early Early Cambrian (one represented by the small skeletal fossils “SSFs” at the base of the Cambrian and the other represented by the succeeding Chengjiang faunas “CFs”), and the other episode as their prelude took place in the “Eocambrian” (i.e. the latest Precambrian), represented by the Ediacaran faunas. This unique Big Bang of life has been recognized as giving birth to the entire morphological Tree Of Animals (or metazoans), in short the TOA. Its “seed” in the deep Precambrian, represented by some sort of protist from which the complete TOA must have grown, remains unknown paleontologically. However, the fossil evidence suggests that the three major episodes of the Cambrian Explosion are responsible for the earliest radiations of the three subkingdoms of animals respectively. While the observed Ediacaran fauna might constitute only a small part of the whole Ediacaran biota, our evidence supports that it was dominated by diploblasts (the “trunk” of the TOA) with only a few possible stem-group triploblasts. The Early Cambrian in turn in two phases explosively yielded almost all the major triploblastic crown-branches (Bilateria: the huge “crown” of the TOA), which include the other two subkingdoms: first the extremely diverse protostomes in the Meishucunian Age and then followed by a nearly entire lineage of early deuterostomes from the Chengjiang, including even its most derived member – the earliest true vertebrates. Among the four most significant milestones of morphological origins and radiations in animal history, the first one (i.e. appearance of metazoans) took place in the Ediacaran Period or earlier times, and the other three can be seen in the windows available from the Chengjiang and the Meishucunian fossil assemblages. The newly discovered extinct Phylum Vetulicolia, which has primitively segmented body with simple gill slits in its anterior division, most probably represents one of the roots of the deuterostome subkingdom. Showing a mosaic of basic features possessed in both the bilateral vetulicolians and some primitive echinoderms, the soft-bodied vetulocystids are best regarded as one of the roots of the extant pentamerous echinoderms. Standing on the “top” of the deuterostome super-branch in the early Cambrian TOA are the “the first fish” Myllokunmingia and Haikouichthys, which bear paired eyes and salient proto-vertebrae. These animals represent the real root of the remainder of the vertebrates or craniates. On the contrary, yunnanozoans, including Yunnanozoon and Haikouella, possess neither eyes nor unequivocal vertebrae, and may have nothing to do with the craniates, let alone the vertebrates. Those enigmatic creatures share a similar body-plan with vetulicolians and should be treated as a side-branch within the lower deuterostomes. 相似文献