化学物质对硬壳蛤幼虫变态的诱导     

张涛  杨红生  周毅  何义朝  张福绥 《海洋科学》2005,29(12):59-67

用KCl、肾上腺素（EPI）、去甲肾上腺素（NE）、L—DOPA和GABA（γ-氨基丁酸）进行了不同浓度不同处理时间对硬壳蛤（Mercenaria mercenaria L ）幼虫变态诱导实验。结果表明，KCl、肾上腺素、去甲肾上腺素和DDOPA对硬壳蛤幼虫的变态均有诱导作用，而GABA的诱导作用不显著。KCl的最佳诱导浓度随处理时间不同而有所不同。处理时间为1～24，48，72h时KCl的最佳诱导浓度分别为33．56，20．13～26．85，13．42mmol／L。肾上腺素和去甲肾上腺素的诱导作用与浓度和处理时间均有关。肾上腺素的最佳处理浓度为100μmol／L，最佳处理时间均为8h，此时幼虫变态率提高最大，为36．97个百分点。当去甲肾上腺素的诱导浓度为100μmol／L，处理时间为8～16h时，幼虫变态率提高也较大，均大于18个百分点，死亡率增加，但均低于30个百分点，当去甲肾上腺素诱导浓度为500μmol／L时，虽然在8～16h的处理时间范围内，幼虫变态提高率也较大，均大于18个百分点，但当处理时间超过8h，在16～48h范围内，幼虫死亡率提高明显增大，均大于50个百分点。L-DOPA的适宜诱导浓度为10～50μmol／L，适宜处理时间为8～24h，此时幼虫变态率均提高30个百分点以上，最高可提高79．43个百分点。GABA的诱导作用较弱，最佳诱导浓度随处理时间的不同而有所不同，处理时间为24h和48h时，最佳诱导浓度为0．1μmol／L；处理时间为0．5～16h时，最佳诱导浓度为100μmol／L。  相似文献   

棉粕蛋白酶解物对异育银鲫(Carassius auratus gibelio)消化、生长和胰蛋白酶mRNA表达量的影响     

刘文斌  王恬 《海洋与湖沼》2006,37(6):568-574

以棉粕蛋白为酶解底物,用枯草杆菌蛋白酶对其进行酶解,以酶解产物1.5%和3.0%两个梯度等量替代鱼饲料配方中棉粕,在室内流水养殖系统中喂养异育银鲫鱼种[体重为(30±2)g]65天。测定鱼的生长、营养物质表观消化率、消化蛋白酶活性及肝胰脏中胰蛋白酶mRNA表达水平等指标。结果表明,添加1.5%和3.0%棉粕酶解产物的鱼在饲养35天后的特定增长率(SGR)分别比对照组高32.5%和56.7%,且差异显著(P<0.05);在饲养65天后,两组特定增长率分别比对照组高8.0%和21.0%,且差异极显著(P<0.01),肝胰脏中胰蛋白酶mRNA表达水平也随棉粕酶解产物添加梯度提高而相应提高,表明鱼的生长与消化蛋白酶mRNA表达水平相关。同时棉粕蛋白酶解物对肠道蛋白酶的活性和营养物质表观消化率都有促进作用,而棉粕蛋白酶解物对鱼肌肉成分并没有改变,这也表明棉粕蛋白酶解物在促进鱼生长、内源酶活性同时并未降低鱼的品质。  相似文献   

东海大规模赤潮危害影响的实验研究——对黑褐新糠虾和卤虫的毒性效应     

颜天  张艺  韩刚  陈洋  周名江 《海洋科学集刊》2007,(48):166-175

The effects of Prorocentrum donghaiense,Karenia mikimotoi,Alexandrium catenella,the causative species of Harmful Algal Blooms in the East China Sea,on the survival and growth of Neomysis awatschensis and Artemia salina were investigated.And the toxicity of eleven strains of Alexandrium----A.lusitanicum(AL-1)、A.minutum(AM-1)、A.spp.(A-K)、A.tamarense(ATHK,ATCI02,ATCI03,AT5-1,AT5-3,AT-6)、A.affine(AS-1,AC-1) to the two above organisms were compared. The results showed that P.donghaiense and K.mikimotoi had no adverse effect on the survival and growth of N.awatschensis and A.salina at 96h.However,the survival rate of N.awatschensis and A.salina declined linearly with the increasing density of A.catenella,and the 96h LC₅₀ for N.awatschensis and A.salina was 4500 and 460 cells/mL,respectively, Five strains of Alexandrium----A.spp,A.lusitanicum (AL-1),A.tamarense(AT5-3,AT-6) and A.affine (AS-1)at the density of 4000cells/mL and 96h had no adverse impacts on N.awatschensis. But other six strains of Alexandrium a.Tamarense(AT5-1,ATHK,ATCI02,ATCI03),A.affine(AC-1) and A.minutum(AM-1)decreased the survival fate of N.awatschensis significantly. Two strains of Alexandrium----A.lusitanicum(AL-1) and A.tamarense(AT-6),at the density of 500cells/mL. had no adverse effects on A.salina in 96h;while the other Alexandrium strains all had adverse effeets on the survival of a.salina, A.salina could increase its length with the increasing density of P.donghaiense,K.mikimotoi and A.luxitanicum. The results indicate that P.donghaiense and K.mikimotoi have nio significant effect on the survival of crustaeean zoop lankton in a short period. But several Alexandrium specised/strains could influence the survival of crustacean zooplankton likely by non-PSP substances. A.salina is shown to be more sensitive than N.awatschensis as test organisms to harmful species.  相似文献   

利用卫星遥感监测南极海冰的研究和进展     

郝春江  解思梅 《海洋预报》1996,13(4):9-14

本文是根据南极“八五”国家攻关课题中的“南极海冰监测和预报”的考核目标，实现为南极考察船在 冰区中航行提供精确和清晰的冰图和预报。  相似文献   

莱州湾海湾扇贝养殖区海水中悬浮颗粒的动态变化   总被引：3，自引：0，他引：3  

梁兴明  方建光  崔毅  唐启升 《海洋与湖沼》2001,32(6):635-640

1997年10月和1998年6月，在莱州湾两个主要海湾扇贝养殖区－芙蓉岛海区和金城海区，各定点进行了24h连续观测和取样，并在实验室内分析了总颗粒物（TPM）、颗粒有机物（POM）和叶绿素α的含量。结果表明，6月份金城海区的颗粒有机物和叶绿素α的含量均高于荚蓉岛海区；在10月份，芙蓉岛海区颗粒有机物的含量高于金城海区，但其叶绿素α的含量却低于金城海区，表明金城海区颗粒有机物的质量高于芙蓉岛海区。  相似文献   

层结流体中的非线性惯性重力内波     

侯一筠  李炜  王新生 《海洋与湖沼》1994,25(5):539-545

根据地球流体力学基本方程组,在密度垂直层结的情况下,引进行波坐标,研究非线性定形波在相平面上的几何拓扑结构。严格论证了不存在定形孤立波,并通过Ｈａｍｉｌｔｏｎ函数及其角作用变把行波系统化成最简形式,由此而得到非线性惯性重力内波的解析解。  相似文献   

Identification of Pb sources in Yellow Sea sediments using stable Pb isotope ratios   总被引：1，自引：0，他引：1  

Man-Sik Choi  Hi-Il Yi  Shou Ye Yang  Chang-Bok Lee  Hyun-Ju Cha 《Marine Chemistry》2007

Rapid economic developments in East Asian countries have inevitably resulted in environmental degradation in the surrounding seas, and concern for both the environment and protection from pollutants is increasing. Identification of sources of contaminants is essential to environmental pollution management. In this study, the provenance of anthropogenic lead (Pb), a major pollutant of Yellow Sea sediments, was determined for river mouth sediments, including those of the Changjiang, Huanghe, Han, and Geum Rivers, and for age-determined shelf core sediments through the measurement of Pb isotope ratios in the HCl-leached fraction using multi-collector inductively coupled plasma-mass spectrometry (MC ICP/MS). Anthropogenic Pb has accumulated in shelf core sediments since 1910, and its isotope ratios were estimated as 0.863–0.866 and 2.119–2.125 for ²⁰⁷Pb/²⁰⁶Pb and ²⁰⁸Pb/²⁰⁶Pb, respectively, from the mixing relationships of the two endmembers. River mouth sediments exhibited enough distinction in anthropogenic Pb isotope ratios to be discriminated: 0.874 (2.144) in the Huanghe, 0.856 (2.129) in the Han, 0.857 (2.122) in the Geum, and 0.854 (2.101) in the Changjiang for ²⁰⁷Pb/²⁰⁶Pb (²⁰⁸Pb/²⁰⁶Pb), respectively. Although isotope ratios of geogenic Pb in sediments dating before 1910 showed narrow ranges (0.842–0.845 and 2.088–2.100 for ²⁰⁷Pb/²⁰⁶Pb and ²⁰⁸Pb/²⁰⁶Pb, respectively), distinct isotope ratios in each core permitted source identification of sediments in the Yellow Sea based on geographic locations and the geogenic Pb of each river. By comparing the isotope ratios of the estimated anthropogenic Pb to source-related materials, the provenances of anthropogenic Pb in Chinese river sediments were presumed to be Chinese coal or ore, which is also a major source of atmospheric particulate Pb. The anthropogenic Pb in the shelf core sediments in the northern Yellow Sea originated from northern Chinese cities such as Beijing and Tianjin through atmospheric pathways. Pb isotope ratios indicated that Pb in Korean river sediments was characteristic of local Korean ores.  相似文献   

Distribution and number change of the Littorinidae (Gastropoda) on intertidal hard bed in the Jiulong River Estuary     

Yi Jiansheng  Li Fuxue 《海洋学报(英文版)》1989,8(1):160-160

There are 4 species of Littorinidae on the intertidal hard bed in the Jiulong River Estu-ary. They are Littorina scabra, L. brevicula, Nodilittorina pyramidalis pyramidalis and N.millegrana. Of these species, L. scabra is a dominant species. According to the results of in-vestigation, we find distribution patterns of the Littorinidae on intertidal hard bed in theestuary as follows: (1)There is no Littorinid species at Haicheng Station. (2)There is only Lit-  相似文献   

不同氮源对微小亚历山大藻生长和毒素产生的影响   总被引：12，自引：0，他引：12  

张清春  于仁诚  周名江  王云峰  颜天  李钧 《海洋学报》2005,27(6):138-145

通过尿素、氯化铵、酵母浸出粉和硝酸钠等氮源对微小亚历山大藻(Alexandrium minutum)生长及毒素产生的影响研究,分析了微小亚历山大藻对不同氮源利用状况的差异.结果表明,在氮饥饿条件下,加入硝酸钠和酵母浸出粉能显著促进微小亚历山大藻的生长;高浓度的氯化铵在加入后对微小亚历山大藻有一定的毒性效应,表现为生长停滞,但毒性效应在5 d后消失,并得到与添加硝酸钠及酵母浸出粉相似的增长速率0.21 d-1;添加尿素对微小亚历山大藻的生长没有显著促进作用.在四种氮源中,尿素对微小亚历山大藻毒素产生的刺激作用也最弱,在稳定期每个细胞藻细胞毒素含量维持在6.00~8.00 fmol;添加硝酸钠、氯化铵和酵母浸出粉的藻细胞在稳定期毒素含量分别达到11.85,12.86和14.64 fmol.硝酸钠和氯化铵刺激藻毒素产生的效果比酵母浸出粉更为直接.四种含氮营养盐对微小亚历山大藻毒素组成的影响都很小.  相似文献   

VR-GIS技术在高压输电线选线中的应用研究——以康定-崇州500kv送电线路为例     

仇文侠  何政伟  许辉熙  赵银兵  姜兰  张怡  李喆 《测绘科学》2008,33(6):202-203,225

VR-GIS技术是一门新兴技术,已经在很多领域得到广泛应用。本文基于ERDAS、ArcGIS、MapGIS等遥感和GIS专业软件,将VR-GIS技术应用到高压输电线选线中,制作康定-崇州500kv送电线路(大杠～沙坪段)走廊带的三维景观飞行模拟系统,再现研究区真实地形、地貌,使工作人员沉浸于虚拟环境中,为其分析和处理问题提供方便,解决因自然环境恶劣造成的外业调查困难等问题。  相似文献