Detection of the end point temperature of thermal denatured protein in fish and chicken meat through SDS-PAGE electrophoresis     

GAO Hongwei）  ）*  MAO Mao）  LIANG Chengzhu）  LIN Chao）    XIANG Jianhai）） Institute of Oceanology  Chinese Academy of Sciences  Qingdao  P. R. China ） College of Food Science  Engineering  Ocean University of China  P. R. China ） Sh  ong Entry-Exit Inspection  Quarantine Bureau of P. R. China  P. R. China 《中国海洋大学学报(英文版)》2009,8(1):95-99

Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was applied in the detection of the end point temperature (EPT) of thermal denatured protein in fish and meat in this study. It was also used in studying the thermal denatured temperature range of proteins in salmon and chicken meat. The results show that the temperature ranges of denatured proteins were from 65 ℃ to 75 ℃ , and these temperature ranges were influenced by the processing methods. Through SDS-PAGE, the features of repeated he...  相似文献   

Vertical amplitude reduction of Rayleigh waves by a row of piles in a poroelastic half‐space     

Yuan‐Qiang Cai  Guang‐Ya Ding  Chang‐Jie Xu  Jun Wang 《国际地质力学数值与分析法杂志》2009,33(16):1799-1821

A row of rigid piles is addressed as the countermeasures for isolating Rayleigh waves in a poroelastic half‐space. The complex characteristic equations for Rayleigh waves are derived via Biot's theory and their existence conditions are given. The piles are modeled as Euler–Bernoulli beams with longitudinal displacements and the diffracted field by each pile is constructed only with Rayleigh waves. Six infinite linear systems of algebraic equations are obtained in terms of the equilibrium of forces and continuity of displacements at the pile–soil interfaces. The systems are subsequently solved in the complex least‐squares sense. The influence of certain pile and soil characteristics such as the permeability of poroelastic soil, spacing between the piles and length of the piles on the isolating performance of a pile barrier is investigated. Computed results show that the permeability of poroelastic soil displays a significant effect on the vertical amplitude reduction of Rayleigh waves. Copyright © 2009 John Wiley & Sons, Ltd.  相似文献   

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张建国  刘晓灿  唐庭梅  马新欣  袁亚红 《大地测量与地球动力学》2009,29(5):47-50

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BTEX异常对海域油气藏指示作用的研究   总被引：2，自引：0，他引：2       下载免费PDF全文

张勇  曹雪晴  孟祥军  刘展  梁源 《海洋学报》2008,30(1):80-85

常规的油气化探方法一般是应用烷烃类作为油气勘探的基本指标,但是烷烃类易受人类活动和生物活动的干扰,使常规的化探技术在油气勘探中一直受到限制。人们开始考虑采用直接的生物标记化合物来预测油气藏,如芳烃系列中的苯系化合物苯(benzene)、甲苯(toluene)、乙苯(ethylbenzene)、二甲苯(xylem),简称BTEX。国内外研究表明,BTEX基本不受人类活动和生物活动的干扰,对油气藏具有良好的指示作用。依据南海某区的实测资料,采用自主研发的油气化探可视化评价软件进行数据处理,剥离背景异常,圈出有效的BTEX异常,并与靶区地层剖面进行了对比,结果证实了BTEX对海域油气藏具有良好的指示作用。  相似文献   

基于数字高程模型不规则三角网的土方计算方法的实现   总被引：1，自引：0，他引：1  

袁婧 《现代测绘》2009,32(2)

本文介绍一种基于数字高程模型(DEM)不规则三角网的土方量计算方法,其利用地形碎部点快速、方便的构建三维实体模型,计算土方精度高.  相似文献   

《昆明市城市排水系统分布专题地图》的编制研究     

王贵武  解智强  周海彬  高忠 《现代测绘》2009,32(3):36-38,42

本文就利用ARCMAP软件编制<昆明城市排水系统分布专题地图>的实践过程,尝试将地图编制与排水管线地理信息系统建设实现很好的结合,实现图-库一体化联接,并最终实现以地下管线探测数据的地图可视化过程,从而完成专业管线专题地图的编制,为城市排水规划、建设与决策提供直观依据.  相似文献   

中国老龄事业发展水平时空演化及其与经济发展水平的空间匹配     

曾通刚  赵媛 《地理研究》2019,38(6):1497-1511

基于“积极老龄化”理论框架,从健康、参与和保障三大维度构建中国老龄事业发展水平评价指标体系。以中国大陆31个省（自治区、直辖市）为研究对象,运用数理统计、重心和空间重叠性、空间错位分析和计量经济模型等方法,分析了2004—2016年中国老龄事业发展水平的时序变化、空间格局、错位特征及其影响机制。结果表明：① 2004—2016年,中国老龄事业发展水平整体快速增长,区域差异逐渐缩小,但区域失衡特征不容忽视。东部地区整体水平高于中部、西部及东北地区,并伴有“西部崛起,中部及东北地区塌陷”特征。东部地区呈现“两极化”现象。② 中国老龄事业基本处于低水平发展阶段,空间分布由西南-东北辐射递减模式转变为显著的“中部塌陷”式,形成一条由北向南的低值集聚带,纵贯中国中部,与区域经济发展存在错位关系。③ 负向错位区主要分布在黄河、长江中上游地区,正向错位区主要集中在东部沿海、长城以北等地区。空间错位强度存在显著的地域差异性,其中西部地区尤为显著。④ 老龄事业发展水平的动态变化是多因素交互作用的结果。经济发展水平、政府政策和行政导向及产业结构的时空分异对其产生正向影响。市场经济规模扩大和人口老龄化未对老龄事业发展起推动作用,与理论预期不符。家庭规模小型化的倒逼作用不显著。  相似文献   

Orientation of Galaxies in the Local Supercluster: A Review     

F. X. Hu  G. X. Wu  G. X. Song  Q. R. Yuan  S. Okamura 《Astrophysics and Space Science》2006,302(1-4):43-59

The progress of the studies on the orientation of galaxies in the Local Supercluster (LSC) is reviewed and a summary of recent results is given. Following a brief introduction of the LSC, we describe the results of early studies based on two-dimensional analysis, which were mostly not conclusive. We describe next the three-dimensional analysis, which is used widely today. Difficulties and systematic effects are explained and the importance of selection effects is described. Then, results based on the new method and modern databases are given, which are summarized as follows. When the LSC is seen as a whole, galaxy planes tend to align perpendicular to the LSC plane with lenticulars showing the most pronounced tendency. Projections onto the LSC plane of the spin vectors of Virgo cluster member galaxies, and to some extent, those of the total LSC galaxies, tend to point to the Virgo cluster center. This tendency is more pronounced for lenticulars than for spirals. It is suggested that ‘field’ galaxies, i.e., those which do not belong to groups with more than three members, may be better objects than other galaxies to probe the information at the early epoch of the LSC formation through the analysis of galaxy orientations. Field lenticulars show a pronounced anisotropic distribution of spin vectors in the sense that they lay their spin vectors parallel to the LSC plane while field spirals show an isotropic spin-vector distribution.  相似文献   

Extensive genetic divergence among Diptychus maculatus populations in northwest China     

孟玮  杨天燕  海萨  马燕武  蔡林钢  马徐发  高天翔  郭焱 《中国海洋湖沼学报》2015,33(3):577-584

D. maculates is a kind of specialized Schizothoracinae fish has been locally listed as a protected animal in Xinjiang Province,China. Ili River located in north of Tianshan Mountain and Tarim River located in north of Qinghai-Tibetan Plateau were two main distribution areas of this fish. To investigate the genetic diversity and genetic structure of D. maculates,four populations from Tarim River system and two populations from Ili River system were collected in this study. A 570-bp sequence of the control region was obtained for 105 specimens. Twenty-four haplotypes were detected from six populations,only Kunes River population and Kashi River population shared haplotypes with each other. For all the populations examined,the haplotype diversity(h) was 0.904 8±0.012 6,nucleotide diversity(π) was 0.027 9±0.013 9,and the average number of pairwise nucleotide differences(k) was 15.878 3±7.139 1. The analysis of molecular variance(AMOVA) showed that 86.31% of the total genetic variation was apportioned among populations,and the variation within sampled populations was 13.69%. Genetic differences among sampled populations were highly significant. F st statistical test indicated that all populations were significantly divergent from each other(P 0.01). The largest F st value was between Yurungkash River population and Muzat River population,while the smallest F st value was between Kunes River population and Kashi River population. NJ phylogenetic tree of D-loop haplotypes revealed two main clades. The neutrality test and mismatch distribution analysis suggested that the fish had went through a recent population expansion. The uplift of Tianshan Mountain and movement of Qinghai-Tibetan Plateau might contribute to the wide genetic divergence of D. maculates in northwest China.  相似文献   

Growth and energy budget of juvenile lenok Brachymystax lenok in relation to ration level     

刘洋  李钟杰  张堂林  苑晶  牟振波  刘家寿 《中国海洋湖沼学报》2015,33(2):347-355

We evaluated the effect of ration level(RL) on the growth and energy budget of lenok Brachymystax lenok. Juvenile lenok(initial mean body weight 3.06±0.13 g) were fed for 21 d at five different ration levels: starvation,2%,3%,4% bwd(body weight per day,based on initial mean values),and apparent satiation. Feed consumption,apparent digestibility,and growth were directly measured. Specific growth rates in terms of wet weight,dry weight,protein,and energy increased logarithmically with an increase in ration levels. The relationship between specific growth rate in terms of wet weight(SGRw,%/d) and RL(%) was characterized by a decelerating curve: SGRw=-1.417+3.166ln(RL+1). The apparent digestibility coefficients of energy exhibited a decreasing pattern with increasing ration level,and there was a significant difference among different RLs. Body composition was significantly affected by ration size. The relationship between feed efficiency rate in terms of energy(FERe) and RL was: FERe=-14.167+23.793RL–3.367(RL)2,and the maximum FERe was observed at a 3.53% ration. The maintenance requirement for energy of juvenile lenok was 105.39 k J BW(kg)-0.80/d,the utilization efficiency of DE for growth was 0.496. The energy budget equation at satiation was: 100IE=29.03FE+5.78(ZE+UE)+39.56 HE+25.63 RE,where IE is feed energy,FE is fecal energy,ZE+UE is excretory energy,HE is heat production,and RE is recovered energy. Our results suggest that the most suitable feeding rate for juvenile lenok aquaculture for wet weight growth is 2.89% bwd,whereas for energy growth,the suggested rate is 3.53% bwd at this growth stage.  相似文献