Rapid detection of virulent protease secreted by Vibrio anguillarum by dot enzyme-linked immunosorbent assay     

ZHANG Zhendong  ZHANG Peijun  MO Zhaolan  WANG Chunling  YU Yang 《海洋学报(英文版)》2005,24(4):155-161

Dot enzyme-linked immunosorbent assay （dot-ELISA）, indirect ELISA and Westem blot were performed to detect the virulent protease secreted by Vibrio anguillarum which was isolated from the diseased left-eyed flounder, Paralichthys olivaceous. Sensitivity results showed that dot-ELISA is a more sensitive, rapid and simple technique for the protease detection. The minimal detectable amount of protease is about 7 pg in the dot-ELISA test, while 7.8 ng in the indirect ELISA and 6.25 ng in the Westem blot respectively. Protease could be detected 2 h after incubation of V. anguillarum in the 2216E liquid medium but enzyme activity was very low at that period. From 6 to 12 h, the amount and enzyme activity of protease increased markedly and reached maximum at stationary phase. Analysis of serum samples periodically collected from the infected flounders showed that after 2 h of infection by V. anguillarum, the pathogenic bacteria could be detected in the blood of the infected flounders but no protease was found. It was 5-6 h after infection that the protease was detected in blood and then the amount increased as infection advanced. Quantitative detection of protease either incubation in the medium or from the blood of infected flounders could be accomplished in virtue of positive controls of quantificational protease standards （＂marker＂） so that the alterations ofprotease secretion both in vitro and in vivo could be understood generally. In addition, the indirect ELISA and dot-ELISA were also performed to detect V. anguillarum cells. Results indicated that the sensitivity of indirect ELISA to bacteria cells is higher than that of the dot-ELISA, and that the minimal detectable amount is approximately 10^4 cell/mL in the indirect ELISA, while 10^5 cell/mL in the dot-ELISA.  相似文献   

Long-term Sensor Drift Found in Recovered Argo Profiling Floats   总被引：5，自引：0，他引：5  

Eitarou?OkaEmail author 《Journal of Oceanography》2005,61(4):775-781

We recovered three Argo profiling floats after 2 to 2.5 years of operation, and recalibrated their temperature, conductivity, and pressure sensors. The results demonstrate that these floats exhibited a significant drift in salinity of −0.0074 to −0.0125, primarily due to the conductivity sensor drift. Combined with the recalibration result for another previously recovered float, the indication is that the negative salinity drift increases nearly in proportion to the operating period of floats. The increasing rate is −0.0041 (±0.0015) year⁻¹, which yields a salinity drift of −0.016 (±0.006) for the expected float lifetime of four years. The present result suggests that reducing the float surfacing time would improve the accuracy of the salinity measurements.  相似文献   

一种准确通用的台风路径预报模式   总被引：1，自引：0，他引：1  

朱敬海  潘杰丽 《海洋预报》2007,24(1):69-74

应用天气学理论,对台风云图的晴空区轴线制约台风移动的规律进行物理解释。根据台风云团与晴空区之间存在的干、湿梯度力和推动台风前进的惯性力的互相关系,建立台风移向预报方程和轨迹预报方程,分析论证了台风移向变化的物理过程,把影响台风移动的复杂因子转化为单一的预报因子。  相似文献   

波浪经过网衣的特性研究   总被引：1，自引：0，他引：1       下载免费PDF全文

宋伟华  梁振林  黄六一  吕永林  陈伯海 《海洋与湖沼》2007,38(1):15-21

依据波浪理论,分析了波浪经过网衣后的流体特性和边界条件,研究了网衣的波浪水动力、波浪透射系数与网衣尺度、特征参数、波浪参数等的关系。设计预加张力放射系泊法进行网衣构件水槽波浪试验。试验构件由10种PE网衣和HDPE框架装配而成,试验工况是规则波,周期为0·8—2·0s,波高为50—250mm。结果表明,作用在网衣上的水平波浪力变化规律与波浪基本一致,呈现周期性和不对称性变化;波浪力变化曲线呈现司托克斯二阶波形状,波峰较陡,波谷较平坦,和波面图相似;网衣水平波浪力周期峰值大约与波高的2·6次方、波长的0·8次方成比例,与网衣宽度l线性相关;随着特征参数d/a增大,网衣波浪力呈线性增加变化,透射系数减少,网衣消浪能力主要集中在网衣水表层。同时设计多层网衣组合的柔性浮式防波堤波浪试验,其消浪能力除了与网衣尺寸、特征参数等相关以外,还与网衣间隔距离有一定的关系。水槽试验时波浪最小透射系数下降到71%左右,从经济、实用角度表明,可用网衣来制作能防护渔业设施的柔性浮式防波堤。  相似文献   

东海原甲藻cDNA文库构建及尝试性EST分析   总被引：2，自引：0，他引：2  

张秀芳  杨官品  刘永建  李瑞香  朱明远 《中国海洋大学学报(自然科学版)》2006,36(3):361-364

东海原甲藻(Prorocentrumdonghaiense)是中国沿海频繁发生的大规模赤潮的原因种之一,大规模分离鉴定东海原甲藻功能基因是理解东海原甲藻赤潮形成过程和机理的重要基础。取对数生长期藻体,经微量总RNA抽提、cDNA合成、cDNA扩增和克隆等步骤,构建了东海原甲藻cDNA文库并进行了尝试性表达序列标签分析。从含有约5000个转化子的文库中随机取150个测序,获得126条EST序列。经网上BlastN及BlastX分析,共发现11个是已知功能的基因的标签。这些基因与东海原甲藻生长发育、物质转换和能量代谢相关。  相似文献   

网格虚拟地理环境体系结构研究     

吴娴  李树军  龚建华  赵德鹏  朱军 《海洋测绘》2006,26(5):71-74

通过将网格技术与虚拟地理环境结合,以服务为核心,构造虚拟数据库,形成网格虚拟地理环境(G ridV irtual Geograph ic Environm ent,G rid-VGE),实现异构数据的集成与共享、任务的分解、闲散资源的充分利用、扩充虚拟地理环境的服务等。在详细阐述了G rid-VGE的框架结构及其关键技术的探讨后,以三维可视化地形漫游为例展示G rid-VGE中的一个应用实例。  相似文献   

南海西部断裂系研究及其物理模拟实验证据   总被引：3，自引：1，他引：3  

孙龙涛  孙珍  詹文欢  孙宗勋  赵明辉  夏少红 《海洋学报》2006,28(3):64-71

南海西部断裂系是由四条典型的走滑断裂所组成的断裂系统,是南海扩张的西部边界.此断裂系主要是在白垩纪以来的印-藏碰撞、新生代印支挤出和南海扩张的共同作用下形成的.通过模拟实验,探讨此断裂系的成因、演化过程,认识到此断裂系经历了五期构造运动.  相似文献   

Studies on Genus Cryptomonas from China seas     

Hu Hongjun  Li Yeguang  Wu Liping  Qi Yuzao 《海洋学报(英文版)》2002,21(4):535-540

ＩＮＴＲＯＤＵＣＴＩＯＮＣｒｙｐｔｏｍｏｎａｓｉｓｕｎｉｃｅｌｌｕｌａｒｂｉｆｌａｇｅｌｌａｔｅｗｈｉｃｈｉｓａｃｏｍｍｏｎｇｅｎｕｓｏｆＣｒｙｐｔｏｐｈｙｔａ .Ｔｈｅｃｅｌｌｓａｒｅｕｓｕａｌｌｙｓｔｒｏｎｇｌｙｃｏｍｐｒｅｓｓｅｄｌａｔｅｒａｌｌｙ .ＴｈｅｒｅａｒｅｏｎｅｏｒｔｗｏｌａｒｇｅＣｈｌｏｒｏｐｌａｓｔｓｔｈａｔｃｏｎｔａｉｎｃｈｌｏｒｏｐｈｙｌｌａ ,ｃ ,ｓｏｍｅｃｈａｒａｃｔｅｒｉｓｔｉｃｃａｒｏｔｅｎｏｉｄｓａｎｄｐｈｙｃｏｂｉｌｉｎｐｉｇｍｅｎｔｓ (Ｈｕｅｔａｌ.,1 997;Ｗｅｉ…  相似文献   

碳水化合物的组合合成     

王群  于广利 《中国海洋大学学报(自然科学版)》2001,31(1):45

碳水化合物的组合合成是一项新兴技术，该技术可以在短时间内合成大量用于进行生物活性筛选的寡糖及拟糖物。液相与固相合成技术可以极大地加快药物研究与开发进程。作者针对该技术在碳水化合物合成方面的研究进展情况进行讨论。  相似文献   

天然热释光技术在海洋油气田勘查中的应用   总被引：18，自引：2，他引：18  

刘海生  程业勋  余慧  朱炳球  ZHU Bing-qiu 《海洋地质与第四纪地质》2001,21(1):107-112

沉积物热释光能有效地反映海洋环境下深部油气田引起的微弱放射性异常。热释光在不同的测试粒级、不同的岩性中的丰度不同，结果为粗粒级＞细粒级、砂岩＞粉砂质砂岩＞泥质粉砂岩＞泥岩。热释光也与部分金属元素含量有关，Ti、Ba、Zn、Fe^3 /Fe^2 与热释光呈正相关关系。热释光与烃类也呈较强的正相关性。不同采用深度热释光测量结果表明，热释光剂量随沉积物的沉积深度和沉积时间的增加而增加，深层样的热释光对油气藏的指示效果更好。  相似文献