海带雌性原生质体的分离与培养     

秦松  王希华  童顺  曾呈奎 《海洋与湖沼》1995,26(S1):126-129

于1993年1月 - 1993年4月，为了制备遗传性纯一的基因工程受体，用酶法从海带雌配子体单性生殖系分离雌性单倍体原生质体。从皱纹盘鲍消化腺及消化器官提取出鲍酶，在25°C下、1％（W／V）的鲍酶与2％（W／V）的纤维素酶（Onozuka R－10）合用能有效解离单细胞和原生质体。海带雌性原生质体经2d培养再生了细胞壁。  相似文献   

南海上层海洋热含量的年际和年代际变化   总被引：1，自引：0，他引：1  

何有海  关翠华 《热带海洋学报》1997,(1)

分析了1959—1988年南海表面至100m垂直平均温度（TAV）资料，结果表明：南海上层海洋热含量存在明显的准两年、4—5年和年代际振动。在E1Nino年，南海上层热含量显著增加。50年代末至70年代初，南海TAV为负距平，此后转为正距平。南海TAV的变化与ENSO事件、东亚冬季风和热带大气环流的变异密切相关。  相似文献   

Static Analysis of Collision Strength of Offshore Platform     

Li Runpei Chen Weigang Gu Yongning Professor  Shanghai Jiao Tong University  Shanghai Assistant  Shanghai Jiao Tong University  Shanghai 《中国海洋工程》1996,(1)

In this paper a numerical analysis method combining FEM incemental technique with limit analysis concept is proposed for the study of the static strength of offshore platform in collision. Large deformation and plasticity are accounted for and the limit yield surface expressed by generalized stress for a tubular section is derived. The modified stiffness matrix of space beam element is formulated by Plastic Node Method. The buckling behavior of beam columns can also be taken into account. It can trace the generation of plastic hinges during loading and finally the ultimate strength of offshore platform against collision is obtained.  相似文献   

Optimal Design for Main Dimensions of a 75000t Floating Production and Storage Vessel     

Tan Jiahua  Ma Wei  Pan Bin Associate Professor  Shanghai Jiao Tong University  Shanghai Lecturer  shanghai Jiao Tong University  Shanghai 《中国海洋工程》1992,(1)

The basis, process and results of the demonstration of the main dimensions of a 75000t floating production and storage vessel are discussed in this paper. A simple but reliable orthogonal design method is applied in the main dimension optimization. The ideas of gradual approximation and feedback from various aspects are put into effect. During the demonstration, in order to make the model tally with the actual situation, the draft design is closely related to the computational analysis, so that the demonstration model can be verified at any time; the handling of the overall system is closely related to the research of each item, which is beneficial not only to the mastery of various regularities, but also to the balance of decisions. Finally, according to the computational results and the regularities obtained from analysis, the main dimensions are determined.  相似文献   

Elasto-Plastic Analysis of Tubular Joints of Offshore Platforms by Finite Element Method     

Chen Tieyun Zhu Zhenghong Professor  Dept. of Naval Architecture  Ocean Engineering  Shanghai Jiao Tong University  Shanghai Lecturer 《中国海洋工程》1991,(1)

The plastic node method is reformulated by the variational principle and is applied to elasto-plastic finite element analysis of tubular joints, eventually including the effect of internal and external gussets, stiffener rings, etc., if necessary. Four different joints are studied here in detail for the elasto-plastic behavior, the strain at the hot spot, the strain concentration factor around the intersection line, and the propagation of the plastic region with loading up to collapse in order to determine the ultimate strength, safety factor, and development of the plastic field. The present results are in good agreement with the experimental results.  相似文献   

The influence of 30~60 d oscillation on the development of the South China Sea summer monsoon     

WEN Zhiping  HE Haiyan  HUANG Ronghui 《海洋学报(英文版)》2004,23(4):569-579

Using the National Center for Enviromental Prediction/National Center for Atmospheric Research (NCEP/NCAR) reanalysis data and NOAA satellite-obser ved outgoing long-wave radiation (OLR) data, the development of the South China Sea (SCS) summer monsoon and intraseasonal (30-60 d) oscillation (ISO) have been examined. The results show that there exists obvious interannual variability of intraseasonal oscillaiton. Using the 16 a time series of filtered OLR averaged over the SCS, an index is defined to define ““onset events““ over the SCS on the ISO time scales. Of the 16 a examined here, 10 shows a strong ISO signal in the onset of monsoon convection over the SCS. In these cases, the ISO initially suppresses the seasonal development of southwesterly and cyclonic circulation over the SCS before the ISO onset. As the ISO propagates northeastward, the low frequency cyclonic circulation anomaly occurs in the SCS and the low frequency southwesterly wind and convection over here dramatically intensify. The northeast progression of the ISO anomaleis plays a role in the initial suppression and then acceleration of the seasonal cycle of the SCS summer monsoon.  相似文献   

Development of a real-time PCR method for Thalassiosira rotula rapid detection     

HE Shanying  YU Zhigang  MI Tiezhu 《海洋学报(英文版)》2007,26(2):133-139

Gene specific primers and DNA probe were designed based on the sequence of 18S rDNA cloned from the red tide alga Thalassiosira rotula. A real-time fluorescent quantitative PCR (RFQ-PCR) method was developed for quantitative detection of T.rotula. The RFQ-PCR assay data showed that the results obtained with the RFQ-PCR quite good agreement with those with the light microscope (LM) counting method, which suggested that the RFQ-PCR could be a useful method for red tide alga detection.  相似文献   

南大洋普里兹湾基于234Th/238U不平衡法的POC输出通量研究   总被引：2，自引：0，他引：2       下载免费PDF全文

何建华  马豪  陈立奇  项宝强  曾宪章  尹明端  曾文义 《海洋学报》2007,29(4):69-76

中国第22次南极科学考察(2005年11月至2006年3月)期间,测定了南极普里兹湾海域5个站位的从表层至150 m水深的不同层位水样中溶解态和颗粒态234Th,238U的放射性比活度以及颗粒有机碳.利用234Th/238U在上层水体中的不平衡,计算了南极普里兹湾上层水体中234Th的平均停留时间和输出通量.结果显示,随着纬度的增加,上层水体中颗粒态和溶解态234Th的平均停留时间总体趋向减小,并在中纬度站位出现了最低值,分别为1~8和29~48 d,而颗粒态和溶解态234Th的输出通量则在中纬度站位出现了最大值,分别为21~38和26~39 dpm/(m3·d).运用箱型清除模式,利用两种不同的方法估算了各水柱中从真光层底部输出的POC通量,平均值分别达到104.7 mmol/(m2·d)(E法)和120.6 mmol/(m2·d)(B法),表明南极普里兹湾夏季存在很高的新生产力,它将会对该海域碳的生物泵过程产生重要作用.  相似文献   

共转换点道集的抽取与转换波时变静校正   总被引：1，自引：0，他引：1  

何兵寿  张会星  彭苏萍 《海洋地质与第四纪地质》2006,26(6):139-144

纵横波速度比固定时,同一地震道中各时间采样点对应不同的转换点位置,因此,常规的整体抽道方法只能抽取特定深度或特定层位条件下的共转换点道集,它不能保证所有P-SV转换波的反射点位置都在同一水平位置处。提出了一种精确抽取共转换点道集的新方法,运用这种方法抽取的共转换点道集中所有数据对应的转换点在地面的投影都对应于同一位置,因此,该道集是一种真正意义上的共转换点道集,在此基础上提出了一种解决短波长问题的转换波时变静校正方法,实际资料处理取得了好的效果。  相似文献   

温差能驱动的水下滑翔器设计与实验研究   总被引：10，自引：1，他引：10  

王树新  王延辉  张大涛  何漫丽  朱光文  任炜 《海洋技术学报》2006,25(1):1-5

论文设计开发了一种新型的温差能驱动的水下滑翔器,并对它做了水域实验研究。文章讨论了温差能驱动的滑翔器运动机理及其在垂直剖面的运动分析,得到稳态运动的参数解。结合能够把水域温差能转变为机械能的热机设计以及滑翔器的主要机械结构和控制硬件系统设计,完成了滑翔器的初步设计与开发。滑翔器在千岛湖进行了水域实验,实验结果表明,此水下滑翔器完全能够利用温差能实现预定的滑翔运动。  相似文献