川纹笛鲷消化道优势菌群PCR-DGGE指纹图谱比较分析   总被引：2，自引：0，他引：2  

周志刚  石鹏君  姚斌  何夙旭  苏永全 《海洋科学》2008,32(11):9-12

采用免培养的16S rDNA梯度凝胶电泳技术（DGGE）对集约化海水网箱养殖川纹笛鲷（Lutjanus sebae）的消化道胃壁、胃内容物、肠壁、肠内容物优势菌群结构进行了比较分析。研究结果显示,川纹笛鲷消化道存在着丰富多样的细菌群落,对DGGE指纹图谱聚类分析表明菌群组成相似度高于55％,其中胃内容物及胃壁细菌组成相似度最高（90％）,这可能与摄食饵料在消化道推移有关;而胃壁与肠壁相似度相对最差,可能反应了由于生理环境不同引起的宿主差异性。通过建立川纹笛鲷消化道16S rDNA-DGGE指纹图谱及比较分析,为澄清川纹笛鲷消化道微生物区系奠定了基础。  相似文献   

上游建库引水对健跳港冲淤影响的经验预测     

于传见  黄世昌  姚炎明  唐子文 《海洋学研究》2008,26(3):53-61

从分析健跳港水文和泥沙特性出发,探讨了健跳港水动力和河床演变的基本特征,结果表明,尽管健跳港内落潮最大流速大于涨潮最大流速,但该港泥沙净输沙方向还是朝向港内;流域径流对健跳港海床稳定起着重要作用,维护健跳港冲淤平衡的主要动力来自洪水期的径流.利用水沙关系和河相关系模型预测了健跳港上游引水后对该港海床淤积的影响.  相似文献   

垂直梯度法与最优分割法确定温跃层边界的比较分析   总被引：2，自引：2，他引：0  

张旭  张永刚  聂邦胜  姚忠山 《海洋通报》2008,27(6)

垂直梯度法是跃层示性特征计算中普遍使用的方法,但在某些情况下并不能很好地描述海洋要素的跃变特征.针对垂直梯度法的不足,引入水团分析中的最优分割法,对典型剖面,以及边界型、逆变型、多层型等几类特殊剖面的跃层边界分别进行了计算,并对垂直梯度法与垂直梯度法的计算结果进行了比较分析,结果表明最优分割法确定的跃层边界更为合理.通过对两种方法确定的中国近海温跃层的整体分布进行比较分析,证明了最优分割法能够弥补垂直梯度法在计算深海与浅海过渡区中的跃层时遇到的标准不匹配问题,较好地保持跃层的连续性.  相似文献   

基于引文分析法的“点-轴系统”理论研究述评     

李满春  姚梦汝  汪侠  刘小强  陈焱明 《地理科学进展》2019,38(2):164-174

论文采用引文分析法,对陆大道院士所著《关于“点-轴”空间结构系统的形成机理分析》一文的349篇引文进行分析,从引文数量、引文作者、发文期刊、研究案例地、学科领域、研究主题、引用动机7个方面,探究“点-轴系统”理论的研究现状。结果表明：自2002年以来,“点-轴系统”理论的引文数量整体呈上升趋势,研究主题聚焦于区域经济和旅游发展;研究案例地以省级和市级中观尺度为主,多为单一型研究区,主要集中在西北丝绸之路经济带、东北辽宁经济圈、西南藏滇川渝、东部长三角四大热点区域;引文质量普遍较高,并且具有多学科交叉应用的特点,但批判性思考和创造性提升相对较少。基于此,论文从拓展研究和实践应用2个角度对“点-轴系统”理论未来发展进行了展望,以期为相关研究提供参考。  相似文献   

Effects of Seawater Acidification on Early Development of Clam Cyclina sinensis     

SUI Yanming  ZHOU Kai  LAI Qifang  YAO Zongli  GAO Pengchen 《中国海洋大学学报(英文版)》2019,(4)

Anthropogenic emission of atmospheric carbon dioxide(CO_2) has led to a rapid increase in atmospheric CO_2 concentration. Increasing atmospheric CO_2 can reduce seawater pH and carbonate ions, which may adversely affect the survival of the larvae of calcareous animals. Cyclina sinensis is a commercially and ecologically important species in several Asian countries. Living in coast shallow waters, this species has experienced the coastal environmental changes frequently throughout its life cycle. In this study, we simulated possible future seawater pH values including 8.2, 7.8 and 7.4 and examined the effects of ocean acidification on the early development of C. sinensis. Clam embryos were incubated for 48 h(2 d) in control and high-CO_2 seawater to compare embryogenesis, larval growth and swimming behavior. Fertilization rate was quite sensitive to pH, and moderate acidification could induce a significant decrease in fertilization rate. However, only extreme acidification could bring significant negative effect to hatching rate, body size, and average path velocity of trochophora. Moreover, with seawater acidification, C. sinensis needs much more time to reach the same developmental stage, which increases the risk of larva survival. Together with recent studies demonstrating negative impacts of high CO_2 on fertilization and larva swimming behavior, the results imply a future decrease of C. sinensis populations in oceans if its acclimation to the predicted environmental alteration does not occur.  相似文献   

Assessing effects of “source-sink” landscape on non-point source pollution based on cell units of a small agricultural catchment     

WANG Jin-liang  CHEN Cheng-long  NI Jiu-pai  XIE De-ti  GUO Xi  LUO Zhi-jun  ZHAO Yue 《山地科学学报》2019,(9)

Ascertaining the relationship between "source-sink" landscape and non-point source(NPS) pollution is crucial for reducing NPS pollution, however, it is not easy to realize this target on cell unit scale. To reveal the relationships between "sourcesink" landscape and NPS pollution based on cell units of a small catchment in the Three Gorges Reservoir Region(TGRR), the runoff and nutrient yields were simulated first by rainfall events on a cell unit scale based on the Annualized AGricultural Non-Point Source Pollution Model(AnnAGNPS). Landscape structure and pattern were quantified with "sourcesink" landscape indicators based on cell units including landscape area indices and locationweighted landscape indices. The results showed that:the study case of small Wangjiagou catchment highlighted a good prediction capability of runoff and nutrient export by the AnnAGNPS model. Throughout the catchment, the spatial distribution trends of four location-weighted landscape indices were similar to the trends of simulated total nitrogen(TN) and total phosphorus(TP), which highlighted the importance of spatial arrangement of "source" and "sink" landscape types in a catchment when estimating pollutant loads. Results by Pearson correlation analysis indicated that the location-weighted landscape index provided a more comprehensive account of multiple factors, and can better reflect NPS-related nutrient loss than other landscape indices applied in single-factor analysis. This study provides new findings for applying the "source-sink" landscape indices based on cell units in small catchments to explain the effect of "source-sink" landscape on nutrient export based on cell unit, and helps improve the understanding of the correlation between "source-sink" landscape and NPS pollution.  相似文献   

Morphological and molecular diagnoses of Polydora brevipalpa Zachs,1933(Annelida: Spionidae) from the shellfish along the coast of China     

YE Lingtong  YAO Tuo  WU Lin  LU Jie  WANG Jiangyong 《中国海洋湖沼学报》2019,(2)

Shell-boring species Polydora brevipalpa Zachs, 1933 is redescribed based on morphological observations and molecular approach for future unambiguous identification. Genetic distance analyses showed that the interspecific polydorid variation(16.7%–25.6%) was at least 15 times higher than the intraspecific one(0.2%–0.9%) based on the cytochrome c oxidase subunit I(CO1) gene sequences of polydorids. However, 18 S rDNA variation pattern demonstrated a rather narrow barcoding gap, with the interspecific polydorid variation(0.5%–5.6%) being very close to the intraspecific one(0.0%–0.4%). As such, the CO1 gene exhibited better DNA barcode for identification of polydorids than the 18 S rDNA gene because of the su ciently large barcoding gaps. Analysis of molecular variance results based on CO1 gene sequences showed that most variations in sequences(97.79%) lay within groups of adult worms and egg capsules rather than between them. This indicated that egg capsules from Crassostrea gigas(Thunberg,1793) in Ningbo and Nantong were related to the adult worms from Patinopecten yessoensis(Jay, 1857) in Dalian, and both of them belonged to P. brevipalpa. This result was further supported by parsimony network analysis, which showed that egg capsules collected from dif ferent localities and adult worms shared a single haplotype. This study was the first to report both P. brevipalpa infestation on C. gigas and to utilise the known CO1 sequences of the adult polydorids to validate morphologically unidentified egg capsules or early larvae. P. brevipalpa was most possibly brought to Chinese waters through transportation of Pa. yessoensis brood stock from Japan.  相似文献   

GDELT数据网络化挖掘与国际关系分析   总被引：2，自引：0，他引：2  

秦昆  罗萍  姚博睿 《地球信息科学学报》2019,21(1):14-24

21世纪以来的国际关系错综复杂、瞬息万变,给世界的经济、安全、外交等带来了深刻变化。这些变化对中国的内外政策产生了重大影响。全面及时地分析国际关系及其变化特征,对于中国的经济和外交发展规划具有重要参考价值。国际关系研究具有复杂性、及时性、时空性等特点,迫切需要时空大数据分析技术为其提供新的思路和技术手段。大众媒体如报纸、广播等记录着世界上发生的各种各样的事件,蕴含着丰富的信息,相对于记录个人活动的社交媒体数据,其更加适合于对人类社会进行大规模和长时间的分析。GDELT是一个免费开放的新闻数据库,它实时监测世界上印刷、广播、网络媒体中的新闻,对其进行文本分析并提取出人物、地点、组织和事件等关键信息。本文利用复杂网络的理论和方法对GDELT进行网络化挖掘并进一步分析国家关系。首先利用该数据构建国家交互网络,然后通过网络特征统计分析国家之间的交互关系,最后探测国家冲突事件交互网络的时序变化。研究发现：① 国家交互网络具有无标度特性,网络连接在整体和局部上都呈现出不均匀性,少数国家与其他国家有大量交互,大多数国家与其他国家的交互很少;一个国家与少数国家有大量交互,而与大多数国家的交互很少。② 国家冲突事件交互网络的突然变化往往对应一些重大事件。本文的研究可以为大数据时代的国际关系探索提供一个新的视角,同时也为新闻媒体数据的分析提供参考。  相似文献   

中国近海藻毒素及有毒微藻产毒原因种调查研究进展   总被引：2，自引：0，他引：2  

梁玉波  李冬梅  姚敬元  金薇  宫长宝  刘仁沿 《海洋与湖沼》2019,50(3):511-524

麻痹性贝类毒素在我国近海污染问题已十分突出,基本呈现逐年加剧的趋势。20世纪90年代,南海麻痹性贝类毒素污染较重; 21世纪初,北黄海麻痹性贝类毒素污染较重;近几年,渤海和福建近海麻痹性贝类毒素污染较重;可产生麻痹性贝类毒素的微藻有亚历山大藻和裸甲藻等。采用小鼠生物法检测我国近海腹泻性贝类毒素超标率32%左右,采用液相色谱/质谱法检测,仅有3起超标的研究报道;现行小鼠生物法检测腹泻性贝类毒素假阳性问题十分突出,应尽快废除;腹泻性贝类毒素均是脂溶性的,脂溶性海洋生物毒素在我国近海常年可检出,偶有虾夷扇贝毒素和鳍藻毒素超标现象。可产生脂溶性毒素微藻有鳍藻和原甲藻及网状原角藻等。失忆性贝类毒素在我国近海常有检出,但无超标现象;产毒微藻有拟菱形藻等。西加鱼毒素在我国南海污染较重,但毒素标准物质的匮乏,限制了西加鱼毒素的调查研究;至今尚未确定产生西加鱼毒素的微藻。酶联免疫吸附法和液相色谱串联质谱法相结合已成为藻毒素快速准确检测成熟的技术,逐渐代替小鼠生物法和液相色谱法。  相似文献   

漠阳江入海口海岸线分维及其机制分析     

李爽  詹文欢  姚衍桃 《海洋通报》2019,38(2):210-216

分维数是反映海岸线复杂程度的客观依据。结合国内外海岸线分形研究进展,本文以漠阳江入海口附近海域为研究区,基于Landsat 8 OLI遥感影像对研究区域提取瞬时水边线,并利用潮汐数据进行校正得到海岸线;利用Fractal Fox2.0计算获得该海岸线的分维数为1.071;通过对研究区的分形机制分析,认为漠阳江附近的断裂构造对海岸起宏观控制作用,从而对海岸线的分维值也起了决定作用。根据断裂体系模型计算得到西侧海岸线的理论分维值为1.089,而对数螺线模型计算得到东侧海岸线的分维值为1.171;实际分维值相对理论值偏小,说明海岸带的沉积与侵蚀影响了分维值。此外,根据本区海岸侵蚀原因的分析,对分维机制进行了探究,初步论证的分维值大小可为海岸的冲淤变化研究提供参考。  相似文献