钝顶螺旋藻部分原生质体及单细胞的制备与培养   总被引：21，自引：0，他引：21  

秦松  王希华  童顺  曾呈奎 《海洋与湖沼》1995,26(1):109-112

于1992年1月-1994年4月，进行超声波处理方法制备钝顶螺旋藻部分原生质体以作为基因工程受体，以及制备单细胞用于固体平板克隆化培养的研究。研究结果表明：超声波以20kHz频率、15W功率作用30s，可使藻丝体断裂成15．0±1．6个细胞长度；延长作用时间，至2－6个细胞长度时，细胞壁结构遭到破坏，形成部分原生质体；继续作用，可形成少量原生质体和大量单细胞。断裂藻丝体、部分原生质体、单细胞以及原生质体均可涂布于固体培养基上再生或生长。以一定密度涂布单细胞与原生质体，能够形成彼此分开的单个克隆，可用于筛选及遗传分析。本文提供了一种节省溶菌酶的制备螺旋藻透性体的方法，超声波作用利于外源基因的导入，而涂布培养利于进一步的筛选和形成克隆。  相似文献   

大地形对正压扰动演变影响     

秦曾灏 《中国海洋大学学报(自然科学版)》1987,(3)

本文着重研究大地形对正压大气迭加在非均匀纬向基本气流上挑动演变的影响。用WKBJ方法推导了正压扰动的频率和频散关系以及波作用量所满足的方程。当基本流场定常且存在无限长的东西向山脉时,则有波作用量守恒。据此,获得了正压不稳定的必要条件:即在所考察的平面域内至少在某点或某些点上含地形效应的某一物理量等于零,扰动就可能得到发展。对波包动能的倚时变化的讨论得知,在无摩擦正压纬向非均匀基流的情形,地形对扰动动能并无直接影响,即导波和曳波分别在西风急流的南侧和北侧得到加强,并分别在西风急流的北侧和南侧强度减弱。扰动的发展由扰动的结构所决定。导波和曳波的波长缩短总伴同其轴线倾向于东西向相联系,而它们的波长伸长总是与其轴线倾向于南北向同时发生。还指出,正压扰动的加强(或减弱)与波长的伸长(或缩短)不是必然的联系,它的成立是有条件的。  相似文献   

应用遗传神经网格方法分析赤潮监测数据   总被引：1，自引：1，他引：1  

杨建强  高振会  孙培艳  石强  李钦亮 《海洋科学进展》2002,20(2):77-82

简要介绍了遗传神经网络方法的原理 ,探讨了应用遗传神经网络方法研究辽东湾海域丹麦细柱藻 (Leptocylindrusdanicus)赤潮与其环境因子之间的关系 ,计算了各环境因子对丹麦细柱藻赤潮的贡献。结果表明 ,温度、盐度、DIN的变化对研究海域丹麦细柱藻种群密度的增长有比较重要的影响 ,DIN是营养限制因子。遗传神经网络是分析赤潮监测数据的有效方法 ,值得进一步探索  相似文献   

藻类分子生物技术两年评─—与藻类活性物质研究有关的生物技术   总被引：4，自引：2，他引：4  

严小军  秦松  曾呈奎 《海洋与湖沼》1997,28(4):440-446

根据１９９２年６月－１９９４年６月国内外发表资料，评述海洋藻类活性物质研究的生物技术及最新成果。认为，海洋生物活性物质研究的重心正转向与海洋生物技术有关的研究。运用生物技术筛选、提取并生产藻类活性物质已经成为这一领域的主流。综合文献表明，藻类活性物质的生物技术正走向成熟，大量的活性物质被发现，光生物反应器将成为主要的下游生产技术，通过藻类基因工程生产药物已初现端倪。  相似文献   

Size-Fractionated Primary Production Estimated by a Two-Phytoplankton Community Model Applicable to Ocean Color Remote Sensing   总被引：3，自引：0，他引：3  

Takahiko?KamedaEmail author  Joji?Ishizaka 《Journal of Oceanography》2005,61(4):663-672

In order to estimate primary production from ocean color satellite data using the Vertical Generalized Production Model (VGPM; Behrenfeld and Falkowski, 1997), we propose a two-phytoplankton community model. This model is based on the two assumptions that changes in chlorophyll concentration result from changes of large-sized phytoplankton abundance, and chlorophyll specific productivity of phytoplankton tends to be inversely proportional to phytoplankton size. Based on the analysis of primary production data, P _opt ^B , which was one parameter in the VGPM, was modeled as a function of sea surface temperature and sea surface chlorophyll concentration. The two-phytoplankton community model incorporated into the VGPM gave good estimates in a relatively high productive area. Size-fractionated primary production was estimated by the two-phytoplankton community model, and P _opt ^B of small-sized phytoplankton was 4.5 times that of large-sized phytoplankton. This result fell into the ranges observed during field studies.  相似文献   

Abnormality of thermal structure and current in the upper western tropical Pacific Ocean and its effect on subtropical high     

Pu Shuzhen  Yu Huiling 《海洋学报(英文版)》1992,11(1):67-82

-Mainly on the basis of the data obtained during PRC/US bilateral TOGA cruises, abnormal variation occurred during the 1986/1987 El Nino is shown in this paper about the thermal structure and circulation of the upper western tropical Pacific Ocean. The effects of the abmormal variation on the subtropical high over the Northwest Pacific Ocean are discussed. During the El Nino: (1) In the east part of the western tropical Pacific Ocean (the subsurface temperature data on the 165° E section are taken as an example), the water wanner than 29 C in the upper layer spread on the longitudinal section and positive temperature anormalies appeared in a large area of the sea surface. (2) In the west part of the western tropical Pacific Ocean (the subsurface temperature data on the 137°E section are representative ), the cross section occupied by the upper layer warmer water ( T >28 ℃ ) became shrunk, and the sea surface temperature showed negative amomalies. (3) The eastward flows in the upper layer of the 165°  相似文献   

Long-term Sensor Drift Found in Recovered Argo Profiling Floats   总被引：5，自引：0，他引：5  

Eitarou?OkaEmail author 《Journal of Oceanography》2005,61(4):775-781

We recovered three Argo profiling floats after 2 to 2.5 years of operation, and recalibrated their temperature, conductivity, and pressure sensors. The results demonstrate that these floats exhibited a significant drift in salinity of −0.0074 to −0.0125, primarily due to the conductivity sensor drift. Combined with the recalibration result for another previously recovered float, the indication is that the negative salinity drift increases nearly in proportion to the operating period of floats. The increasing rate is −0.0041 (±0.0015) year⁻¹, which yields a salinity drift of −0.016 (±0.006) for the expected float lifetime of four years. The present result suggests that reducing the float surfacing time would improve the accuracy of the salinity measurements.  相似文献   

Simple determination of dimethylsulfide (DMS) and dimethylsulfoniopropionate (DMSP) using solid-phase microextraction and gas chromatography-mass spectrometry     

Takushi?NikiEmail author  Taiki?Fujinaga  Mariyo?F.?Watanabe  Junji?Kinoshita 《Journal of Oceanography》2004,60(5):913-917

Solid-phase microextraction (SPME) is a simple, sensitive and less destructive method for the determination of dimethylsulfide (DMS) in seawater. Combined with detection by gas chromatography-mass spectrometry (GC-MS), the method had sufficient sensitivity (minimum detectable concentration of DMS was 0.05 nM), and practical levels of reproducibility (relative standard deviation ≤7%) and linearity (r ² > 0.995) over a wide concentration range (0.5 to 910 nM). The protocol developed was applied to a Sagami Bay water sample to determine concentrations of DMS and DMSP, and in situ DMSP-lyase activity.  相似文献   

Eddy Shedding from the Kuroshio Bend at Luzon Strait   总被引：16，自引：1，他引：16  

Yinglai?JiaEmail author  Qinyu?Liu 《Journal of Oceanography》2004,60(6):1063-1069

TOPEX/POSEDIENT-ERS satellite altimeter data along with the mean state from the Parallel Ocean Climate Model result have been used to investigate the variation of Kuroshio intrusion and eddy shedding at Luzon Strait during 1992–2001. The Kuroshio penetrates into the South China Sea and forms a bend. The Kuroshio bend varies with time, periodically shedding anticyclonic eddies. Criteria of eddy shedding are identified: 1) When the shedding event occurs, there are usually two centers of high Sea Surface Height (SSH) together with negative geostrophic vorticity in the Kuroshio Bend (KB) area. 2) Between the two centers of high SSH there usually exists positive geostrophic vorticity. These criteria have been used to determine the eddy shedding times and locations. The most frequent eddy shedding intervals are 70, 80 and 90 days. In both the winter and summer monsoon period, the most frequent locations are 119.5°E and 120°E, which means that the seasonal variation of eddy shedding location is unclear.  相似文献   

红海湾水域溶解氧、pH值、盐度和营养盐分布特征及相互关系研究   总被引：10，自引：0，他引：10  

王小平  贾晓平  林钦  李纯厚  甘居利  蔡文贵  王增焕  吕晓瑜 《海洋通报》1999,18(5):35-40

通过１９９８年６月对广东省红海湾海水养殖水域环境因子的调查,阐述了海水中溶解氧、盐度、ｐＨ值和营养盐的分布特征及其变化规律,并讨论了它们之间的相互关系,结果表明,溶解氧及海水ｐＨ值随温度由北（除１５￣１７号站）向南（除６号站）增高而增高。同时证实了光合作用是导致溶解氧含量和ｐＨ值增高的主要原因。而营养盐与盐度呈密度的负相关,浮游植物总量随营养盐由北向南降低而明显增高。  相似文献