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Induction of hepatic cytochrome P450-dependent microsomal mono-oxygenase by xenobiotics is a well-established phenomenon in teleost fish. As in laboratory mammals, fish possess multiple forms of cytochrome P450 that display overlapping substrate specificity. One such isoform, CYP1A1, which has been cloned and sequenced from rainbow trout, has been shown to be orthologous to rat CYP1A1 and, as in mammals, is inducible up to several hundred-fold by planar aromatic hydrocarbons, PCBs and dioxins. It has been suggested that induction of CYP1A1 orthologues might provide a sensitive biomonitor for environmental pollution by mixtures of such compounds. In the current study, polyclonal antibodies directed against CYP1A1 purified from rat and trout liver were used to monitor induction of the CYP1A1 orthologue in hepatic microsomes from the fresh water species, the channel catfish (Ictalurus punctatus). Catfish from a local fish farm were induced in the laboratory by three daily injections of 50 mg/kg of the PCB mixture Aroclor 1254 and compared with fish taken from a site in central Arkansas—the Bayou Meto, known to be polluted with dioxin. Hepatic microsomal activities towards ethoxyresorufin (EROD) and pentoxyresorufin (PROD) were measured and Western blot analysis carried out with the two antibodies. EROD was elevated in both the Aroclor-treated fish and in the Bayou Meto fish compared with untreated fish farm controls; smaller but significant increases were observed in PROD. Spearman's rank correlations of 0·74 and 0·89 were observed between EROD and immunoquantified cross-reactivity towards the rat CYP1A1 and trout CYP1A1 antibodies.  相似文献   
124.
The aim of this work is to give a summary of the work on Cs-137 in Swedish lakes carried out mainly by our group (the Liming-mercury-caesium project) between 1986 and 1990. The focus is on results from extensive field experiments carried out in 41 lakes testing various remedial measures to speed up the natural recovery of Cs-137 in lakes: Lake liming and wet land liming with primary rock lime, sedimentary rock lime and so-called mixed lime, which also contains nutrients; potash treatment and intensive fishing. Selected results: The remedies have given the intended water-chemical response. None of the methods used works effectively as cure, i.e., no rapid and clear reduction in the concentrations of radioactive caesium in fish is obtained in comparison with lakes where the waterchemical or biological conditions are not changed. In lakes with long water turnover time and with low values of, foremost, conductivity, hardness and potassium, the fish had relatively higher concentrations at the same fallout levels. The differences present between the lakes as regards the continued magnitude of the change in concentration in fish can foremost be linked to factors controlling the secondary load (i.e., the internal loading and the input from the catchment). A successful potash treatment (in oligotrophic lakes) may imply that the natural recovery will be at the most 5% faster compared to no treatment. This would give quite positive implications in the long run since the ecological half-life for Cs-137 in pike (the top predator in these lake types) is very long. The time interval between the remedies and the latest fish analyses (about 2 years on average) is not sufficient to obtain (statistically) clear-cut results on these the small effects of the remedies. A longer time series of data is required for this.  相似文献   
125.

Knowledge of upper ocean currents is needed for trajectory forecasts and is essential for search and rescue operations and oil spill mitigation. This paper addresses effects of surface waves on ocean currents and drifter trajectories using in situ observations. The data set includes colocated measurements of directional wave spectra from a wave rider buoy, ocean currents measured by acoustic Doppler current profilers (ADCPs), as well as data from two types of tracking buoys that sample the currents at two different depths. The ADCP measures the Eulerian current at one point, as modelled by an ocean general circulation model, while the tracking buoys are advected by the Lagrangian current that includes the wave-induced Stokes drift. Based on our observations, we assess the importance of two different wave effects: (a) forcing of the ocean current by wave-induced surface fluxes and the Coriolis–Stokes force, and (b) advection of surface drifters by wave motion, that is the Stokes drift. Recent theoretical developments provide a framework for including these wave effects in ocean model systems. The order of magnitude of the Stokes drift is the same as the Eulerian current judging from the available data. The wave-induced momentum and turbulent kinetic energy fluxes are estimated and shown to be significant. Similarly, the wave-induced Coriolis–Stokes force is significant over time scales related to the inertial period. Surface drifter trajectories were analysed and could be reproduced using the observations of currents, waves and wind. Waves were found to have a significant contribution to the trajectories, and we conclude that adding wave effects in ocean model systems is likely to increase predictability of surface drifter trajectories. The relative importance of the Stokes drift was twice as large as the direct wind drag for the used surface drifter.

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126.
Flakket on the island of Anholt in Denmark is a cuspate foreland facing the microtidal Kattegat sea. It is composed of a number of beach ridges typically covered by dune sand and separated by swales and wetlands. OSL dating indicates that the evolution of Flakket began c. AD 1000. Foreland growth was punctuated by a major episode of coastal reorganization leading to coastal retreat c. AD 1800. Coastal retreat led to the formation of an erosion surface that separates older and higher‐lying beach‐ridge and swale deposits from younger and lower‐lying deposits. The palaeo‐sea level is deduced from the architecture of the deposits, and interpretation of ground‐penetrating radar data and geomophological observations indicates that relative sea level was about 1.90±0.25 m above present sea level c. AD 1000, but about 0.00±0.25 m relative to present sea level c. AD 1830 and c. AD 1870. Anholt is situated at the margin of the uplifted Fennoscandian area; assuming uplift to be about 1.2 mm a?1 it follows that absolute sea level was about +0.70±0.25 m at AD 1000, but around ?0.22±0.25 m at AD 1830 and around ?0.17±0.25 m at AD 1870. Within the uncertainties of the age control, the sea‐level indicators mapped by ground‐penetrating radar reflections and the variability of estimates of uplift found in the literature, the result obtained for AD 1000 is consistent with findings from the Stockholm area in Sweden and with a recently published global sea‐level curve.  相似文献   
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Here we investigate the use of optically stimulated luminescence (OSL) for dating cobbles from the body of successive beach ridges and compare cobble surface‐derived ages to standard quartz OSL ages from sand. Between four and eight cobbles and sand samples (age control) were dated with the luminescence method, taken from the modern beach and from beach ridges on the south and north extremes of a prograding spit on the westernmost coast of Lolland, Denmark. Luminescence‐depth profiles perpendicular to the surfaces of the cobbles show that the feldspar infrared signals stimulated at 50 °C were fully reset to various depths into the cobbles prior to final deposition; as a result, the equivalent doses determined from close to the surface of such cobbles can be used to calculate burial ages. Beach‐ridge burial ages given by the average of ages of individual cobbles taken from the same site are consistent, within errors, with the ages derived from the sand samples. Cobble‐ and sand‐derived ages show that the southernmost beach ridge at Albuen was formed around 2 ka ago, indicating that this sandy spit is younger than other coastal systems in Denmark. The agreement between ages derived from clasts and from standard quartz OSL in this study confirms that, even in the absence of sandy sediments, we can reliably date sites using OSL by targeting larger clasts. In addition, the record of prior light exposure contained in the shape of the cobbles’ luminescence‐depth profile removes one of the major uncertainties (i.e. the degree of signal reset prior to burial) in the luminescence dating of high latitude sites.  相似文献   
129.
The Cenozoic succession of Browse Basin is characterized by a carbonate system, that developed from a non-tropical ramp in Eocene-lower Miocene times to a tropical rimmed platform in the middle Miocene. The evolution of the platform was unraveled through the interpretation of the seismic geomorphology and borehole data of the middle Miocene tropical reef system. The first reef structures developed during the early middle Miocene as narrow linear reef belts with an oblique orientation with respect to shelf strike direction. Subsequently, they prograded toward the platform margin to form a barrier reef with a minimum length of 40 km. The barrier reef itself comprises three distinct ridges separated by progradational steps. The second and third step are separated by a karstified horizon, which is interpreted to represent the global sea-level fall shortly before the Serravallian/Tortonian boundary. The following third ridge formed in a slightly downstepped position during the sea-level lowstand and initial transgressive phase. Further sea-level rise during the early Tortonian first drowned the barrier-reef system and subsequently also the patch reefs and relic atolls that had established in a backstepped position in the platform interior. The similar evolution of the Browse Basin reef system and other contemporaneous carbonate systems indicates a strong impact of eustatic sea-level changes. Relatively large subsidence rates in the study area possibly augmented the eustatic sea-level rise in the Tortonian and hence contributed to the drowning of the reef system. However, the initiation and final demise of the reef system was also governed by global and regional climate variations. The first seismically-defined reefs developed simultaneous to a maximum in the transport capacity of the Indonesian throughflow, which brings warm low-salinity waters to the North-West Shelf. Reef drowning followed the restriction of this seaway close to the middle to early Miocene boundary. This near closure of the Indonesian seaway possibly led to a regional amplification of the global middle to late Miocene cooling trend and hampered the potential of the reef system to keep up with the rising sea-level.  相似文献   
130.
Field studies in the framework of the EU funded BEEP project (Biological Effects of Environmental Pollution in Marine Coastal Ecosystems, 2001-2004) aimed at validating and intercalibrating a battery of biomarkers of contaminant exposure and effects in selected indicator species in the Mediterranean, the North Atlantic and the Baltic Seas. Major strategic goals of the BEEP project were the development of a sensitive and cost-efficient biological effects monitoring approach, delivery of information and advice to end-user groups, and the implementation of a network of biomarker researchers around Europe. Based on the main results obtained in the Baltic Sea component of the BEEP the present paper summarises and assesses the applicability of biomarkers for different regions and species in this sea area. Moreover, a general strategy and some practical considerations for the monitoring of biological effects in the Baltic Sea are outlined.  相似文献   
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