红树林根际土壤中耐高盐苯酚降解菌的分离鉴定          下载免费PDF全文

曹宏明  龚斌  朱丽娟  潘贵妮  刘飞琪  李世盛 《应用海洋学学报》2021,40(2):179-188

苯酚对海洋生态环境具有较大的威胁,从红树林根际土壤中分离获得对环境具有高适应性的高效芳烃降解菌具有重要的意义。本研究对北部湾红树林根际土壤中的苯酚降解菌进行富集培养,经过分离纯化得到30株菌株,筛选得到5株菌株FGYS1、FGYS7、FGYS11、FGYB1和FGYB5,可耐受70 g/L NaCl的高盐环境,最高可降解1 500 mg/L的苯酚。采用形态观察、BOX-PCR菌种多样性分析、16S rRNA序列分析等方法对菌株进行鉴定,均为假单胞菌属(Pseudomonas)。菌株FGYS1、FGYS7和FGYS11为同一种假单胞菌,与Pseudomonas extremaustralis 14-3T和Pseudomonas meridiana CMS 38~T的16S rRNA序列相似性分别达到99.71%和99.64%。菌株FGYB1和FGYB5为同一种假单胞菌,分别与Pseudomonas veronii DSM 11331~T和Pseudomonas extremaustralis 14-3~T的16S rRNA序列相似性均达到99.64%。16S rRNA序列的系统发育树分析表明菌株FGYS1、FGYS7、FGYS11、FGYB1和FGYB5在进化上与P. veronii为同一个分支,而与假单胞菌属其他模式菌株的序列进化关系相距较远。菌株FGYS1降解苯酚的适宜温度为25～35℃、适宜pH为4～6、最高耐NaCl含量为60 g/L;菌株FGYB1降解苯酚的适宜温度为25～35℃、适宜pH为4～6、最高耐NaCl含量为100 g/L,两株菌株对pH、温度和盐度的适应范围较广。菌株FGYS1和FGYB1还可以同时降解邻苯二酚、对苯二酚和间苯二酚。本研究分离得到的苯酚降解菌在应对红树林芳烃污染和生态环境修复方面显示了较大的潜力。  相似文献   

国外浅水反水雷的发展探究          下载免费PDF全文

任斌 《数字海洋与水下攻防》2021,4(6):525-528

水雷是具有战略威慑作用的常规兵器,在历次海战中均发挥着巨大作用,特别是在登陆作战时, 浅水水雷是抗登障碍物重要组成部分,能否快速清扫水雷直接影响着登陆作战的成败。海军在未来执行作战任务时,将不可避免遭遇到浅水水雷的威胁。针对浅水反水雷的需求,我国应加快推进浅水反水雷装备的研制,提升登陆作战时的反水雷能力。  相似文献   

卡里马塔海峡贯穿流与印尼贯穿流的相互作用     

李淑江  徐腾飞  孙俊川  杨丽娜  滕飞  王冠琳  王永刚 《海洋科学进展》2021,39(2):197-209

卡里马塔海峡贯穿流将中国南海的低盐水输运到爪哇海,与印度尼西亚贯穿流(印尼贯穿流)携带的西太平洋高盐水在印度尼西亚海(印尼海)交汇,二者通过混合、浮力强迫等过程相互作用。这改变了印度尼西亚海的水体热盐性质,影响局地海气交换和热带太平洋-印度洋之间的热盐交换。依据卡里马塔海峡、龙目海峡和望加锡海峡的实测表层海流数据,采用被动示踪法和数值模拟诊断实验,分析并研究了2支海流在季节尺度上的相关关系及其相互作用。观测结果表明,卡里马塔海峡贯穿流与印尼贯穿流的表层流季节变化存在负相关,且超前1个月达到相关系数最大值,其中印尼贯穿流下游的龙目海峡表层流与卡里马塔海峡贯穿流关系更密切。被动示踪结果显示,卡里马塔海峡释放的示踪粒子主要向南流入爪哇海,然后经巽他海峡、龙目海峡及班达海方向流出,但很难进入到望加锡海峡北部;望加锡海峡释放的表层示踪粒子主要经龙目海峡和班达海流入印度洋,但无法进入到爪哇海及卡里马塔海峡。数值诊断实验结果表明,卡里马塔海峡贯穿流在冬季阻挡了印尼贯穿流表层海水的南下,从而使其表层流产生明显的季节变化;而望加锡海峡贯穿流对卡里马塔海峡贯穿流的影响较小。在季节尺度上,卡里马塔海峡贯穿流对印尼贯穿流尤其是表层流,有着重要影响,但印尼贯穿流对卡里马塔海峡贯穿流的影响较小。  相似文献   

印度尼西亚北苏拉威西红树林生态系统碳库研究     

陈顺洋  陈彬  SASTROSUWONDO Pramudji  DHARMAWAN I Wayan Ek  欧丹云  尹希杰  俞炜炜  陈光程 《海洋学报(英文版)》2018,37(12):85-91

近年来的研究指出红树林在海岸带碳固定和碳储存方面发挥着重要的作用。尽管印度尼西亚的红树林面积在全球占很大的比重，对于该地区红树林的有机碳储量和土壤有机碳来源的认识仍有限。本研究调查了印度尼西亚北苏拉威西海洋型的Wori红树林中生态系统有机碳储量及其空间分布特征，以及土壤有机碳的来源，以期加深该地区红树林“蓝碳”功能的认识。研究结果显示，Wori红树林0-50cm深度土壤中有机碳储量为15.4 kg/m²，占生态系统碳储量的主要部分（65%）。红树植物生物量和生态系统碳储量分别为8.3 kg/m²和23.7 kg/m²。土壤有机碳储量在不同离岸距离的采样站位中未表现出显著的空间分布差异，而生物量碳储量则在外滩最高。¹³C稳定同位素分析结果表明红树林土壤中蓄积的有机碳主要来源于红树林有机质，而潮水中的悬浮有机质和红树林外缘的海草并不构成红树林土壤有机碳的重要来源，它们的贡献者都低于20%。研究结果进一步证实了热带地区海洋型红树林湿地在碳储存以及红树植物对碳固定方面的重要性。  相似文献   

阿勒泰地区植被覆盖度及ET对气温变化的响应   总被引：1，自引：0，他引：1       下载免费PDF全文

韩飞飞  闫俊杰  郭斌 《干旱区地理》2019,42(6):1436-1444

研究气温对植被覆盖度和ET (Evapotranspiration，ET)的影响，对干旱区应对气候变化、维系生态系统稳定具有重要意义。基于阿勒泰地区及周边7个气象站，CRU数据集中的气温数据及MODIS ET数据，采用Mann-Kendall非参数检验、植被盖度反演等方法，对阿勒泰地区气温变化对植被覆盖度及ET的影响进行了研究。结果表明：（1）在1901—2016年过去的116 a间，阿勒泰地区年平均气温以0.18 ℃·(10 a)^-1速率增加，在1982年由突变前的2.2 ℃增加到突变后的3.5 ℃。（2）2000—2017年阿勒泰地区植被覆盖度变化的空间差异明显，植被覆盖度增加的面积与降低的面积总体相当；全区66.71%的区域植被覆盖度变化与气温呈负相关，而呈正相关的比例仅占18.55%，且全区气温变暖而盖度降低区域的占比达31.71%。（3）2000—2016年阿勒泰地区ET总体呈降低趋势，整个区域61.65%的面积温度降低、ET降低，而19.92%的区域表现为温度增加而植被ET降低。  相似文献   

Numerical Simulation on Flow Past Two Side-by-Side Inclined Circular Cylinders at Low Reynolds Number     

Liu  Cai  Gao  Yang-yang  Qu  Xin-chen  Wang  Bin  Zhang  Bao-feng 《中国海洋工程》2019,33(3):344-355

A series of three-dimensional numerical simulations is carried out to investigate the effect of inclined angle on flow behavior behind two side-by-side inclined cylinders at low Reynolds number Re=100 and small spacing ratio T/D=1.5 (T is the center-to-center distance between two side-by-side cylinders, D is the diameter of cylinder). The instantaneous and time-averaged flow fields, force coefficients and Strouhal numbers are analyzed. Special attention is focused on the axial flow characteristics with variation of the inclined angle. The results show that the inclined angle has a significant effect on the gap flow behaviors behind two inclined cylinders. The vortex shedding behind two cylinders is suppressed with the increase of the inclined angle as well as the flip-flop gap flow. Moreover, the mean drag coefficient, root-mean-square lift coefficient and Strouhal numbers decrease monotonously with the increase of the inclined angle, which follows the independent principle at small inclined angles.

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紫刺参早期生长发育与体色形成的研究     

陈相堂  赵斌  李成林  韩莎  胡炜 《海洋科学》2019,43(6):95-101

本研究对紫刺参胚胎和幼体发育过程进行了显微观测,比较分析了其幼体早期生长情况与体色形成过程。结果表明:(1)在水温21.0±0.2℃条件下,紫刺参受精卵在受精10～15min后释放第一极体,5h 30min～6h 30min进入囊胚期, 18～20h发育成原肠胚; 30～34h进入耳状幼体阶段, 8～10d变态发育为樽形幼体, 12～14d发育成稚参;紫刺参胚胎和幼体发育时序与普通刺参无显著差异。(2)紫刺参早期发育中的樽形幼体发生率和附着变态率分别为58.4±4.7%和45.1±2.7%,高于普通刺参。(3)紫刺参浮游阶段后期随日龄增长表现出较普通刺参发育更快的优势,在日龄6～7d表现出显著差异(P0.05)。(4)紫刺参稚参在日龄35d前后自背部开始着色,50d～60d腹部开始着色,110d体表紫色转变近乎成参体色,完成变色过程。  相似文献   

高分三号SAR影像双阈值变化检测   总被引：1，自引：1，他引：0  

崔斌  张永红  闫利  魏钜杰 《遥感学报》2020,24(1):1-10

双阈值合成孔径雷达SAR(Synthetic Aperture Radar)变化检测算法具有在发现变化区域的同时还能确定地表发生后向散射变化类型的优点。针对广义高斯双阈值最小误差法D-GKIT(Dual Generalized Kittler and Illingworth Thresholding)在进行阈值选取时直方图中不同类别像素灰度级重叠严重时,分割结果容易在尖峰单侧选取出双阈值而导致无法正确分割差异图的问题,本文提出一种结合归一化最大类间方差和广义高斯最小误差法GKIT(Generalized Kittler and Illingworth Thresholding)的双阈值SAR变化检测方法。首先,提出以归一化最大类间方差值作为灰度级重叠程度的判别参数,确定阈值的选取顺序及两个候选区间;然后,利用GKIT在候选区间内进行分割,获取单侧阈值及非变化类拟合函数;最后,提出利用非变化类拟合函数更新后的直方图作为另一侧阈值选取基础进行分割,得到对应分割阈值。以宁波地区高分三号(GF-3)SAR卫星影像作为试验研究数据,结果表明:本文方法能较好地解决灰度级重叠时D-GKIT无法进行正确分割的问题,具有良好的变化检测效果和更强的鲁棒性且达到了利用研究区数据验证利用GF-3号SAR卫星影像进行变化检测研究可行性的目的。  相似文献   

Exploring the stemflow dynamics and driving factors at both inter- and intra-event scales in a typical subtropical deciduous forest     

Haixia Zhang  Congsheng Fu  Aimin Liao  Can Zhang  Jiufu Liu  Niu Wang  Bin He 《水文研究》2021,35(3):e14091

Numerous efforts have been made to understand stemflow dynamics under different types of vegetation at the inter-event scale, but few studies have explored the stemflow characteristics and corresponding influencing factors at the intra-event scale. An in-depth investigation of the inter- and intra-event dynamics of stemflow is important for understanding the ecohydrological processes in forest ecosystems. In this study, stemflow volume (F_V), stemflow funnelling ratio (F_R), and stemflow ratio (F_%) from Quercus acutissima and Broussonetia papyrifera trees were measured at both inter- and intra-event scales in a subtropical deciduous forest, and the driving factors, including tree species and meteorological factors were further explored. Specifically, the F_V, F_R and F_% of Q. acutissima (52.3 L, 47.2, 9.6%) were lower than those of B. papyrifera (85.1 L, 91.2, 12.4%). The effect of tree species on F_V and F_% was more obvious under low intensity rainfall types. At the inter-event scale, F_V had a strong positive linear correlation with rainfall amount (G_P) and event duration (D_E) for both tree species, whereas F_R and F_% had a positive logarithmic correlation with G_P and D_E only under high-intensity, short-duration rainfall type. F_R and F_% were mainly affected by wind speed and the maximum 30-min rainfall intensity under low-intensity, long-duration rainfall type. At the intra-event scale, for both tree species, the mean lag time between the start of rainfall and stemflow was the shortest under high-intensity, short-duration rainfall type, while the mean duration and amount of stemflow after rain cessation were the greatest under high-amount, long-duration rainfall type. The relationship between stemflow intensity and rainfall intensity at the 5-min interval scale also depended greatly on rainfall type. These findings can help clarify stemflow dynamics and driving factors at both inter- and intra-event scales, and also provide abundant data and parameters for ecohydrological simulations in subtropical forests.  相似文献   

A two-layer model for studying 2D dissolved pollutant runoff over impermeable surfaces     

Taotao Zhang  Yang Xiao  Dongfang Liang  Hongwu Tang  Junzeng Xu  Saiyu Yuan  Nairu Wang  Bin Luan 《水文研究》2021,35(5):e14152

Dissolved pollutants in stormwater are a main contributor to water pollution in urban environments. However, many existing transport models are semi-empirical and only consider one-dimensional flows, which limit their predictive capacity. Combining the shallow water and the advection–diffusion equations, a two-dimensional physically based model is developed for dissolved pollutant transport by adopting the concept of a ‘control layer’. A series of laboratory experiments has been conducted to validate the proposed model, taking into account the effects of buildings and intermittent rainfalls. The predictions are found to be in good agreement with experimental observations, which supports the assumption that the depth of the control layer is constant. Based on the validated model, a parametric study is conducted, focusing on the characteristics of the pollutant distribution and transport rate over the depth. The hyetograph, including the intensity, duration and intermittency, of rainfall event has a significant influence on the pollutant transport rates. The depth of the control layer, rainfall intensity, surface roughness and area length are dominant factors that affect the dissolved pollutant transport. Finally, several perspectives of the new pollutant transport model are discussed. This study contributes to an in-depth understanding of the dissolved pollutant transport processes on impermeable surfaces and urban stormwater management.  相似文献