Diagnosis of iridovirus in large yellow croaker by PCR   总被引：1，自引：0，他引：1  

Chen Xinhu  Wang Xiaowen  Wu Wenzhong 《海洋学报(英文版)》2003,22(4):635-642

A rapid and sensitive PCR-based method for the detection of the large yellow croaker iridovirus(LYCIV) is described,which involves the amplification of a 295 bp fragment of the LYCIV ATPase gene from DNA isolated from naturally infected fish spleen.Sequencing of LYCIV ATPase gene fragment showed it shared 100% nucleotide sequence homology with the corresponding region of the ATPase gene of red sea bream iridovirus(RSIV) and sea bass iridovirus(SBIV),suggesting that LYCIV was homologous with RSIV and SBIV at least in part of the gemone.The specificity and sensitivity of the PCR procedure were tested on the iridovirus-infected fishes,the expected fragment was detected from spleen DNA samples of infected fishes,whereas no fragments were amplified from healthy fish spleen DNA,white spot syndrome baculoviruses(WSBV) DNA and pseudorabies virus(PRV) DNA.Detection limit of this method was 10^-7 ng positive plasmid DNA containing target sequence,equal to about 100 virions.In the infected experiment,first positive detection(1/4) appeared at Day 3 post-infection,all fish(4/4) tested positive at Day 7,however obvious symptoms were observed at Day 8,so LYCIV infection could be detected prior to the appearance of obvious symptoms.These results indicate that this PCR method could be used for early,rapid and specific detection of LYCIV infection.  相似文献   

福州市地热田地面沉降机理及对策     

周伟  刘用泉  吴文飞  周峥嵘  范德江 《中国海洋大学学报(自然科学版)》1994,(Z3)

本文从地质地层结构、新构造运动和人为（过量抽取地下热水）两方面因素探讨地面沉降的形成机理，并提出控制地面沉降的对策。  相似文献   

Studies on Genus Cryptomonas from China seas     

Hu Hongjun  Li Yeguang  Wu Liping  Qi Yuzao 《海洋学报(英文版)》2002,21(4):535-540

ＩＮＴＲＯＤＵＣＴＩＯＮＣｒｙｐｔｏｍｏｎａｓｉｓｕｎｉｃｅｌｌｕｌａｒｂｉｆｌａｇｅｌｌａｔｅｗｈｉｃｈｉｓａｃｏｍｍｏｎｇｅｎｕｓｏｆＣｒｙｐｔｏｐｈｙｔａ .Ｔｈｅｃｅｌｌｓａｒｅｕｓｕａｌｌｙｓｔｒｏｎｇｌｙｃｏｍｐｒｅｓｓｅｄｌａｔｅｒａｌｌｙ .ＴｈｅｒｅａｒｅｏｎｅｏｒｔｗｏｌａｒｇｅＣｈｌｏｒｏｐｌａｓｔｓｔｈａｔｃｏｎｔａｉｎｃｈｌｏｒｏｐｈｙｌｌａ ,ｃ ,ｓｏｍｅｃｈａｒａｃｔｅｒｉｓｔｉｃｃａｒｏｔｅｎｏｉｄｓａｎｄｐｈｙｃｏｂｉｌｉｎｐｉｇｍｅｎｔｓ (Ｈｕｅｔａｌ.,1 997;Ｗｅｉ…  相似文献   

Experimental investigation on a two-part underwater towed system     

Jiaming Wu  Allen T. Chwang 《Ocean Engineering》2001,28(6)

An experimental set-up is developed and proved to be effective for laboratory study of an underwater towed system. The experimental technique gives a practical method for monitoring the kinematic and dynamic performance of an underwater towed system in a ship towing tank. Both the theoretical and experimental results in the investigation indicate that the hydrodynamic response of a towed vehicle to the wave induced motion of a towing ship can be significantly reduced by applying a two-part tow method. A comparison of the numerical and experimental results in the investigation demonstrates that the numerical simulation results are close to the experimental data, overall agreement between experimental and theoretical results is satisfactory. The results qualitatively verify the mathematical model of a two-part underwater towed system proposed by Wu and Chwang [Wu, J., Chwang, A.T., 2000. A hydrodynamic model of a two-part underwater towed system. Ocean Engineering 27 (5), 455–472].  相似文献   

盐藻和β-胡萝卜素研究述评   总被引：9，自引：1，他引：9  

刘建国  吴超元 《海洋与湖沼》1995,26(3):23-330

养殖盐藻生产β-胡萝卜素是近十几年新兴起的研究领域，因其应用前途广内，现已成为微藻生物学的研究前沿和热点，预计本世纪末和下世纪初将有更大发展。鉴于我国在这方面文章不系统、不全面，本文从盐藻生物学、养殖学、β-胡萝卜素异构体、β-胡萝卜素形成生理等角度全面概述了该领域的研究现状，并提出存在的问题与研究方向。  相似文献   

黑潮和中国近海环流   总被引：9，自引：0，他引：9  

伍伯瑜 《台湾海峡》1991,10(1):25-32

本文根据历史资料总结和讨论了黑潮对中国近海环流影响的基本结果,指出经巴士海峡进入南海和沿台湾海峡向北运动的黑潮分支,其年际变化明显,并认为该黑潮分支的运动状况对中国近海特别是南海和东海南部的中、下层环流影响显著,需要作进一步的调查研究。  相似文献   

夜光藻的室内培养   总被引：3，自引：1，他引：3  

吴玉霖  周成旭  张永山 《海洋与湖沼》1994,25(2):165-167

夜光藻的室内培养对于研究夜光藻的生理生态特性及其增殖机理是一个关键性的环节。通过试验，发现在实验室条件下以扁藻作为饵料，夜光藻能良好的生长和繁殖，从而为夜光藻的室内培养解决了关键性的饵料问题，实现了国内夜光藻室内的长期培养。  相似文献   

二色桌片参酶解液对转化细胞的效应   总被引：3，自引：2，他引：3  

陈正明  陈玉强  曹顺达  吴乔  苏文金 《台湾海峡》1997,16(3):270-274,T001

用二色桌片参（Mensamariaintercedens）酶解液处理转化的人胚肺成纤维细胞（NLF）后，细胞生长受到显著抑制，抑制率达64．84％，光镜下细胞透光性加强，铺展较好，细胞核形更为规则，核畸形现象减少；线粒体结构趋于正常；软琼脂集落实验表明，细胞集落形成率由23ｘ10－5下降至12．5ｘ10－5，裸小鼠致瘤实验显示抑瘤率为82．39％，差异显著（p＜5％）。结果提示，二色桌片参酶解液可能逆转转化的HLF细胞的恶性表型，对其有一定的诱导分化作用。  相似文献   

A proposed directional function and wind-wave directional spectrum   总被引：4，自引：0，他引：4  

Wen Shengchang  Wu Kejian  Guan Changlong  Sun Shicai  Zhang Dacuo 《海洋学报(英文版)》1995,14(2):155-166

Ａｐｒｏｐｏｓｅｄｄｉｒｅｃｔｉｏｎａｌｆｕｎｃｔｉｏｎａｎｄｗｉｎｄ－ｗａｖｅｄｉｒｅｃｔｉｏｎａｌｓｐｅｃｔｒｕｍ￥ＷｅｎＳｈｅｎｇｃｈａｎｇ；ＷｕＫｅｊｉａｎ；ＧｕａｎＣｈａｎｇｌｏｎｇ；ＳｕｎＳｈｉｃａｉａｎｄＺｈａｎｇＤａｃｕｏ（Ｒｅｃｅｉ...  相似文献   

养殖对虾暴发性流行病—中国对虾肝胰腺坏死病研究   总被引：3，自引：0，他引：3  

吴友吕  倪梦麟 《海洋学研究》1993,11(4):42-48

1993年我国沿海各省市养殖对虾发生了暴发性流行病,造成了极大的损失。本文报导我们在浙江省舟山市调查和研究的结果,发现肝胰腺坏死杆状病毒和弧菌并发感染是引起对虾死亡的主要原因。  相似文献