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壳聚糖酶高产菌株的筛选和发酵条件的研究 总被引:6,自引:0,他引:6
从采集的土样中分离到1株产壳聚糖酶能力较强的菌株OU01,并对OU01进行了16S rDNA序列、形态及生理生化鉴定分析。16S rDNA序列分析表明该菌属于微杆菌属;该菌的形态特征与生理生化鉴定结果表明该菌与Microbacteriumsp.的相似性最高;因此将其初步鉴定为微杆菌属(Microbacterium)。同时对该菌的发酵条件进行了初步研究:该菌的最适培养基组成为(g/L):chitosan 10,(NH4)2SO420,MgSO4.7H2O 1.3,K2HPO4.3H2O 1.4,Glucose 1,Yeast extract 3,NaCl 5,起始pH=6.30。最适发酵温度为30℃,最佳发酵时间为96 h,在上述最优条件下,该菌株产酶达到118 U/mL。Microbacteri-umsp.OU01菌株为壳聚糖酶的研究和应用提供了新的来源。 相似文献
24.
The VOF method and the k–ε model, combined with the equation of state of air at constant temperature, have been used to calculate the total horizontal wave force caused by monochromatic waves acting on a perforated caisson with a top cover. From comparison of various parameters, such as the total horizontal force, the pressure difference on the front wall, the pressure on the back wall and the pressure on the top cover, between the numerical results and test data, it can be seen that the numerical results agree well with the test data. It is concluded that the method described in this paper can be utilized to calculate wave forces acting on perforated caissons with a top cover in the case of nonovertopping, nonbreaking waves. A simplified method to calculate the total horizontal force has been developed, based on test data, using a least-squares method. A comparison between the numerical results and the values calculated from the simplified equations shows good agreement. Therefore the simplified equations can be used in engineering applications to evaluate the total horizontal force on a perforated caisson with a top cover. 相似文献
25.
大陆岩石圈在张裂和分离时的变形模式 总被引:4,自引:0,他引:4
姚伯初 《海洋地质与第四纪地质》2002,22(3):59-67
通过对南海南北共轭边缘地壳剖面的对比研究,发现大陆岩石圈的物理性质是分层的:上、中地壳呈脆性,下地壳表现出塑性,而岩石圈上地幔则仍呈脆性。因此,在它受张性应力场作用时,其变形和破裂分离方式也是分层进行的:上、中地壳能发生犁式断裂,产生的断块沿断面转动在地表产生一系列半地堑,并使地壳厚度减薄;如拉张应力继续作用时,上、中地壳将沿犁式断裂被拉开,从而形成上、下板块边缘,并彼此分开。下地壳则发生塑性变形,使地壳厚度减薄,并最终将其拉断。岩石圈上地幔亦可产生陡倾断裂,形成的断块沿断面转动亦使其厚度减薄,并最终沿陡倾断裂被拉断。这就是我们称之为岩石圈变形和破裂分 离时的分层变形及分层破裂分离模式。 相似文献
26.
Tan Jiahua Ma Wei Pan Bin Associate Professor Shanghai Jiao Tong University Shanghai Lecturer shanghai Jiao Tong University Shanghai 《中国海洋工程》1992,(1)
The basis, process and results of the demonstration of the main dimensions of a 75000t floating production and storage vessel are discussed in this paper. A simple but reliable orthogonal design method is applied in the main dimension optimization. The ideas of gradual approximation and feedback from various aspects are put into effect. During the demonstration, in order to make the model tally with the actual situation, the draft design is closely related to the computational analysis, so that the demonstration model can be verified at any time; the handling of the overall system is closely related to the research of each item, which is beneficial not only to the mastery of various regularities, but also to the balance of decisions. Finally, according to the computational results and the regularities obtained from analysis, the main dimensions are determined. 相似文献
27.
The incompressible viscous uniform and shear flow past a circular cylinder is studied. The two-dimensional Navier-Stokes equations are solved by a finite element method. The governing equations are discretized by a weighted residual method in space. The stable three-step scheme is applied to the momentum equations in the time integration. The numerical model is firstly applied to the computation of the lid-driven cavity flow for its validation. The computed results agree well with the measured data and other numerical results. Then, it is used to simulate the viscous uniform and shear flow over a circular cylinder for Reynolds numbers from lO0 to lO00. The transient time interval before the vortex shedding occurs is shortened considerably by introduction of artificial perturbation. The computed Strouhal number, drag and lift coefficients agree well with the experimental data. The computation shows that the finite element model can be successfully applied to the viscous flow problem. 相似文献
28.
珠江口和琼东南盆地天然气水合物形成和稳定分布的地球化学边界条件及其分布区 总被引:22,自引:0,他引:22
通过对南海北部陆缘珠江口和琼东南盆地气田的天然气形成水合物的地球化学计算模拟及地质地球化学条件分析,对珠江口和琼东南盆地天然气形成水合物的地球化学边界条件及分布区进行了研究。认识到南海北部陆缘琼东南和珠江口盆地内的断裂构造是天然气向海底渗漏的通道,为天然气水合物在海底的形成提供了物源;盆地内巨厚的第四纪富有机质沉积也为天然气水合物形成提供了充足的细菌成因生物气源。在海底温度2-16℃范围内,琼东南盆地气田10种天然气和珠江口盆地气田18种天然气形成水合物的压力有比较大的范围,随温度增高,天然气水合物形成的压力增高;盆地间和各天然气样品之间形成水合物的压力均是不一致的。在南海海水平均盐度3.4%条件下,结合海底温度与水深变化资料,珠江口和琼东南盆地天然气水合物形成和稳定分布的海区是不同的,珠江口盆地小于230m水深的海区没有天然气水合物的形成,在230-760m水深的海区可能有天然气水合物的存在,天然气水合物的稳定分布区应该在大于860m水深的深水区;在琼东南盆地水深小于320m的海区不可能有天然气水合物的形成,在320-650m水深的海区可能有天然气水合物的存在,大于650m水深的海区是天然气水合物的稳定分布区。 相似文献
29.
AbstractBased on hydrological data obtained during the 7th to 9th Chinese National Arctic Research Expeditions in the summers of 2016–2018, the main water structure on the shelf of the northern Bering Sea and the volume and heat fluxes of the Bering Strait throughflow were analyzed. Distinct variability was identified in the three Pacific water masses feeding the strait - Anadyr Water (AW), Bering Shelf Water (BSW) and Alaskan coastal water (ACW). Overall, the temperature and salinity of the entire section increased each year, with 2018 showing significant anomalies, i.e., a temperature anomaly of up to 1?°C and a maximum salinity anomaly of 2. From 2016 to 2018, the extent of the ACW gradually narrowed in the eastern part of this section, while the AW expanded eastward each year. The net volume transport through each of the three sections increased poleward from 1.65?Sv to 2.76?Sv, with the AW increasing from 0?Sv to 1.03?Sv, the BSW varying between 0.52–1.65?Sv, and the ACW gradually decreasing from 1.04?Sv to disappearing completely. The net heat fluxes were also poleward, varying between 25.77 TW and 61.50 TW, and showing a significant increase. Significant variations in magnitude and extent were observed in each water mass of the Bering Strait throughflow, which could produce widespread effects in the Arctic Ocean and the global ocean beyond. 相似文献
30.
2004年10月至2005年8月,对不同季节千岛湖蚤状潘的垂直分布情况以及昼夜迁移进行了研究。结果表明,蚤状潘在千岛湖分布广泛,春季和夏季蚤状潘主要分布在15-25m水层,而在秋冬季分布相对均匀,从表层到60m水深都有分布;比较了蚤状漫在不同季节的迁移现象,春季和秋季蚤状潘为夜间迁移模式,而在夏季和冬季虽然都存在迁移现象,但不同于常见的三种迁移模式。 相似文献