固定化鲅鱼乙酰胆碱酯酶的制备及部分性质测定   总被引：3，自引：0，他引：3  

孟范平  何东海  朱小山  马冬冬  杨正先  YANG Zheng-Xian 《中国海洋大学学报(自然科学版)》2005,35(6):1067-1071

酶的固定化是酶传感器制备过程中重要的1个环节.研究采用直接共价法固定鲅鱼乙酰胆碱酯酶（AChE）.制备方法为：0.1g CNBr活化的Sepharose 4B凝胶用1mmol/L的HCl充分溶胀后,与活力为10U的AChE溶液混合,于4℃下150r/min振荡8h.所制备的固定化酶活力回收率较高（96%）,对pH值和温度变化的适应能力均优于非固定化酶;在3个月保存期内,前者的活力损失13%,而后者的活力则下降89%.这说明固定过程能够大大提高AChE的抗逆性和保持酶活力的稳定,有利于酶传感器的制备.  相似文献   

法国“阿塔郎特”号海洋调查船上的重力取样     

程振波  刘振夏  王揆洋  崔金瑞 《海洋科学进展》1997,(3)

在海洋地质调查中，如何取得好的沉积物样品是每一位海洋地质学家所向往的，而获得好的沉积物样品的关键又很大程度上取决于先进的海洋调查仪器的使用。法国“阿塔郎特”号海洋调查船上的重力取样管不管在性能上，还是安全性上都瞰称世界上比较先进的取样设备。  相似文献   

On low-pass digital filters in oceanography   总被引：1，自引：0，他引：1  

Wang Ji  Ralph T. Cheng 《海洋学报(英文版)》1993,12(2):183-196

-Two types of filters are widely used to remove semidirunal and diurnal tidal signals and other high frequency noises in oceanography. The first type of filters uses moving average with weights in time domain, and can be easily operated. Some data will be lost at each end of the time series, especially for the low low-pass filters. The second type of filters uses the discrete Fourier transform filter (DFTF) which operates in the frequency domain, and there are no data loss at the ends for the forward transform. However, owing to the Gibbs phenomenon and the discrete sampling (Nyquist effect) , ringing appears in the inverse transformed data, which is especially serious at each end. Thus some data at the ends are also discarded. The present study tries to find out what causes the ringing and then to seek for methods to overcome the ringing. We have found that there are two kinds of ringings, one is the Gibbs phenomenon, as defined before. The other is the "Nyquist"ringing due to sampling Nyquist critical  相似文献   

A New Approach to High-Order Boussinesq-Type Equations with Ambient Currents   总被引：6，自引：3，他引：6  

王亚玲张洪生  缪国平朱良生 《中国海洋工程》2005,19(1):49-60

A new approach to high-order Boussinesq-type equations with ambient currents is presented. The current velocity is assumed to be uniform over depth and of the same magnitude as the shallow water wave celerity. The wave velocity field is expressed in terms of the horizontal and vertical wave velocity components at an arbitrary water depth level. Linear operators are introduced to improve the accuracy of the kinematic condition at the sea bottom. The dynamic and kinematic conditions at the free surface are expressed in terms of wave velocity variables defined directly on the free surface. The new equations provide high accuracy of linear properties as well as nonlinear properties from shallow to deep water, and extend the applicable range of relative water depth in the case of opposing currents.  相似文献   

海洋声层析的基本原理和应用     

朱芸  吕连港 《海洋科学进展》2002,20(4):70-75

从射线声学和简正波声学的角度，概述了海洋声层析的基本理论，包括射线走时反演、简正波走时反演、简正波相位反演和简正波水平折射层析。海洋声层析以反演海水温度和流速为基础。还总结了声层析在海洋学研究中的应用。  相似文献   

An Approach to Stability Analysis of Embedded Large-Diameter Cylinder Quay   总被引：3，自引：3，他引：3  

WANG  Yuanzhan 《中国海洋工程》2002,16(3):383-393

The large-diameter cylinder structure, which is made of large successive bottomless cylinders placed on foundation bed or partly driven into soil, is a recently developed retaining structure in China. It can be used in port, coastal and off-shore works. The method for stability analysis of the large-diameter cylinder structure, especially for stability analysis of the embedded large-diameter cylinder structure, is an important issue. In this paper, an idea is presented that is, em-bedded large-diameter cylinder quays can be divided into two types, i.e. the gravity wall type and the cylinder pile wall type. A method for stability analysis of the large-diameter cylinder quay of the cylinder pile wall type is developed and a method for stability analysis of the large-diameter cylinder quay of the gravity wall type is also proposed. The effect of sig-nificant parameters on the stability of the large-diameter cylinder quay of the cylinder pile wall type is investigated through numerical calculation.  相似文献   

摄食水平和性别对稀有鮈鲫生长和能量收支的影响   总被引：7，自引：0，他引：7  

朱晓鸣  解绶启  崔奕波  杨云霞  光寿红 《海洋与湖沼》2001,32(3):240-247

于1995年10月采集本所自繁有ju鲫（约1.03g/ind)，在30℃水温下进行摄食－生长实验，研究了从饥饿到饱食5个摄食水平对不同性别稀有ju鲫的生长和能量收支的影响，结果表明，鱼体干物和能量含量不受摄食率和性别的影响，随着摄食率的增加，雌鱼的湿重，干重和能量特定生长率均呈双对数增加，雄鱼湿重特定生长率呈双对数增加，而干物质和能量特定生长率呈指增加，雄鱼生长速度及摄食率低于雌鱼，随着摄食率的增加，食物能损失于粪便中的比例均呈上升趋势，损失于排泄物中的比例变化不大，呈下降趋势，用于代谢的比例呈下降趋势，用于生长的比例呈上升趋势，除饥饿组外，在各个摄食水平，雌鱼食物能用于代谢的比例较雄鱼低，而用于生长的比例较雄鱼高。  相似文献   

黄河三角洲桩西101站潮沟地貌形态及其水动力学研究   总被引：6，自引：1，他引：6  

尹延鸿  成国栋  范顺庭  王玉荣  朱兰部 《海洋科学》1997,21(5):46-49

在潮沟下游，横跨且生趣与潮沟的横断面设了５个观测站，进行了２４ｈ的水动力测量。结果表明，涨落潮流速流向不对称，在潮沟内，涨落潮流流速差别很大，涨潮流流速最大为９ｃｍ／２，而落潮流流速最大可达３８ｃｍ／ｓ。据推测，潮沟内之所以落潮流流速明显大于涨潮流流速，可能是由于潮沟与岸线斜交有关。  相似文献   

The Mathematical and Parameterized Expressions for Wave Induced Excess Flow of Momentum     

朱首贤丁平兴  张文静 《中国海洋工程》2007,21(1):137-146

Wave induced excess flow of momentum(WIEFM)is the averaged flow of momentum over a wave period due to wave presence,which may also be called 3-D radiation stress.In this paper,the 3-D current equations with WIEFM are derived from the averaged Navier-Stokes equations over a wave period,in which the velocity is separated into the large-scale background velocity,the wave particle velocity and the turbulent fluctuation velocity.A concept of wave fluctuating layer(WFL)is put forward,which is the vertical column from the wave trough to wave ridge.The mathematical expressions of WIEFM in WFL and below WFL are given separately.The parameterized expressions of WIEFM are set up according to the linear wave theory.The integration of WIEFM in the vertical direction equals the traditional radiation stress(namely 2-D radiation stress)given by Longuet-Higgins and Stewart.  相似文献   

台风Haitang(0505)登陆过程地形影响的数值模拟试验     

张建海  陈红梅  诸晓明 《海洋通报》2006,25(2):1-7

以2005年严重影响我国的登陆台风Haitang为研究对象,在用MM5模式成功模拟台风登陆前后路径、强度、降水以及结构特征的基础上,通过地形敏感性试验,分析了地形对台风的影响及其机理。结果表明:台风在经过台湾及其后在福建登陆期间的异常路径是地形诱生低压发生发展的结果;在降水强度上东部沿海特殊地形对台风北侧东南气流的辐合抬升使得暴雨增幅度1倍以上;地形对台风强度也有一定影响,约为4～5hPa。通过对有无地形影响时台风结构的比较发现地形影响主要在低层,使得高度场分裂两个中心,低层台风主体受山脉阻挡在位置上落后于高层。分析还发现台风登陆前夕移动方向西侧相当位温向西倾斜也可能与地形有关。  相似文献