南海沉积物细菌胞外蛋白酶在家族水平上的多样性   总被引：2，自引：0，他引：2  

杨金玉  冯阳阳  陈秀兰  解彬彬  张玉忠  石梅  张熙颖 《海洋学报(英文版)》2019,38(12):73-83

海洋产蛋白酶细菌及其分泌的胞外蛋白酶在降解有机氮以推动海洋氮循环进行方面发挥着重要作用，但目前对这二者多样性的认识非常有限。本研究自南海沉积物中筛选得到90株产蛋白酶细菌，并通过N-端氨基酸序列，分析了其胞外蛋白酶在家族水平上的多样性。16S rRNA基因序列分析的结果表明，筛选的产蛋白酶细菌均属于Gammaproteobacteria纲，且大多数属于Alteromonadales目和Vibrionales目的不同属。对其中14株菌株的14个胞外蛋白酶的N-端氨基酸序列分析表明，所有这些蛋白酶属于金属蛋白酶的M4家族或丝氨酸蛋白酶的S8家族。本研究提供了海洋沉积物产蛋白酶细菌在类群及其胞外蛋白酶在类型上的新细节，这将有助于全面了解微生物酶促降解海洋沉积物有机氮的过程和机理。  相似文献   

Dissolved organic matter in surface runoff in the Loess Plateau of China: The role of rainfall events and land-use     

Zhongwu Li  Hao Peng  Binggeng Xie  Chun Liu  Xiaodong Nie  Danyang Wang  Mei Huang  Haibing Xiao  Lin Shi  Xuqin Zhang  Jieyu Jiang 《水文研究》2020,34(6):1446-1459

Exploring the chemical characterization of dissolved organic matter (DOM) is important for understanding the fate of laterally transported organic matter in watersheds. We hypothesized that differences in water-extractable organic matter (WEOM) in soils of varying land uses and rainfall events may significantly affect the quality and the quantity of stream DOM. To test our hypotheses, characteristics of rainfall-runoff DOM and WEOM of source materials (topsoil from different land uses and gullies, as well as typical vegetation) were investigated at two adjacent catchments in the Loess Plateau of China, using ultraviolet–visible absorbance and excitation emission matrix fluorescence with parallel factor analysis (PARAFAC). Results indicated that land-use types may significantly affect the chemical composition of soil WEOM, including its aromaticity, molecular weight, and degree of humification. The PARAFAC analysis demonstrated that the soils and stream water were dominated by terrestrial/allochthonous humic-like substances and microbial transformable humic-like fluorophores. Shifts in the fluorescence properties of stream DOM suggested a pronounced change in the relative proportion of allochthonous versus autochthonous material under different rainfall patterns and land uses. For example, high proportions of forestland could provide more allochthonous DOM input. This study highlights the relevance of soils and hydrological dynamics on the composition and fluxes of DOM issuing from watersheds. The composition of DOM in soils was influenced by land-use type. Precipitation patterns influenced the proportion of terrestrial versus microbial origins of DOM in surface runoff. Contributions of allochthonous, terrestrially derived DOM inputs were highest from forested landscapes.  相似文献   

黑潮流域海温适应性观测敏感区诊断方法研究     

崔宝龙  笪良龙  过武宏  韩梅 《海洋科学》2018,42(7):80-86

敏感区诊断是适应性观测的关键问题,集合变换卡尔曼方法(EnsembleTransformKalmanFilter,ETKF)是目前主要的诊断方法之一。将集合变换卡尔曼方法应用于海洋环境适应性观测,根据ROMS海洋模式数据构建海表温度集合预报,以黑潮流域宫古海峡附近海域为验证区进行敏感区诊断计算,分析不同间隔时间条件下敏感区分布情况,结合模拟系统观测试验验证在敏感区进行适应性观测对预报质量的提升效果。结果表明,在诊断所得敏感区内添加观测能够提升预报质量;随时间间隔增大,敏感区向上游区域平移且预报质量提升效果减小;与在验证区整体添加观测相比,敏感区观测对预报质量提升效果基本相同并且观测成本明显减少。  相似文献   

中国海洋无脊椎动物分类学与系统演化研究进展与展望     

李新正  寇琦  王金宝  甘志彬  杨梅  龚琳  隋吉星  马林  曲寒雪  初雁凌  曾宥维  王伟娜  张祺  董栋 《海洋科学》2020,44(7):26-70

综述了我国海域无脊椎动物的分类学和系统演化研究的历史和概况，以及我国分类系统学工作者在海洋无脊椎动物分类学、区系与动物地理学、系统发育与分子系统学领域的主要工作，重点介绍了中国科学院海洋研究所的海洋无脊椎动物分类学工作。涉及类群包括原生动物、海绵动物、刺胞动物、线虫、多毛类环节动物、星虫、螠虫、软体动物、节肢动物、苔藓动物、毛颚动物、棘皮动物、半索动物等主要的无脊椎动物门类。涉及海域以我国管辖海域，特别是中国近海为主，也涉及了西太平洋、西南印度洋等深海环境的无脊椎动物类群的分类学报道。本文总结过去，展望未来，对于在我国在海洋无脊椎动物分类与系统演化研究领域成就基础上，发现薄弱环节，研讨今后本学科的发展方向，填补研究空白，赶超本领域国际前沿，都有重要借鉴意义。  相似文献   

One-dimensional consolidation of soil under multistage load based on continuous drainage boundary     

Yi--> Tian  Wenbing Wu  Guosheng Jiang  M. Hesham El Naggar  Guoxiong Mei  Meijuan Xu  Rongzhu Liang 《国际地质力学数值与分析法杂志》2020,44(8):1170-1183

In engineering practice, a rapid loading rate can result in ground failure when the strength of soft soils is relatively low, and a multistage loading scheme is always utilized to deal with this situation. Firstly, under a multistage load and the continuous drainage boundary, an analytical solution of excess pore-water pressure and consolidation degree is obtained by virtue of the superposition formula of excess pore-water pressure, and a more general continuous drainage boundary under arbitrary time-dependent load is developed. Then, a comparison with existing analytical solutions is conducted to verify the present solution. A preliminary attempt on applying the continuous drainage boundary into the finite element model is made, and the feasibility of the numerical model for the one-dimensional consolidation under the continuous drainage boundary is verified by comparing the results calculated by FEM with that from present analytical solution. Finally, the consolidation behavior of soil is investigated in detail for different int erface parameters or loading scheme. The results show that, in land reclamation projects, a horizontal drain should be placed close to the boundary with a smaller interface parameter to improve the consolidation efficiency. The degree of consolidation is also related to the applied time-dependent load and interface parameters.  相似文献   

Psammoma bodies in two types of human ovarian tumours: a mineralogical study     

Meng Fanlu  Wang Changqiu  Li Yan  Lu Anhuai  Mei Fang  Liu Jianying  Du Jingyun  Zhang Yan 《Mineralogy and Petrology》2015,109(3):357-365

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On the development of a coupled regional climate–vegetation model RCM–CLM–CN–DV and its validation in Tropical Africa     

Guiling Wang  Miao Yu  Jeremy S. Pal  Rui Mei  Gordon B. Bonan  Samuel Levis  Peter E. Thornton 《Climate Dynamics》2016,46(1-2):515-539

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Re-Os pyrite geochronology of Zn-Pb mineralization in the giant Caixiashan deposit,NW China     

Dengfeng Li  Huayong Chen  Pete Hollings  Li Zhang  Mei Mi  Jie Li  Jing Fang  Chengming Wang  Wanjian Lu 《Mineralium Deposita》2016,51(3):309-317

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广西田东县那矿金矿区地质与地球化学特征及找矿方向     

黄美 《地质与勘探》2016,52(5):854-864

广西田东县那矿金矿位于滇黔桂金三角,属于卡林型金矿。本文在对那矿金矿区进行1∶10000地质填图的基础上,基本上查明了其成矿地质背景,结合1∶10000土壤地球化学测量,选取Au、As、Sb三种元素为成矿指示元素。通过土壤地球化学数据分析,对矿区内指示元素异常的特征、分布与地质现象进行对比分析,进行异常解释评价。研究结果显示:那矿金矿区中三叠统百逢组中上部粉砂质泥岩、泥岩、泥质粉砂岩是赋矿层位;坡表谷背斜与坡表谷断裂的复合部位为成矿有利部位;硅化、(褐)黄铁矿化、毒砂矿化、高岭石化、绢云母化、碳酸盐岩化等蚀变为金的矿化标志;圈定的Ⅰ号异常带是矿致异常,其Au元素异常规模大、品位高,是该区找矿的重点靶区。  相似文献   

大兴安岭北段东坡中北部小莫尔可地区 中生代火山岩成因及其地质意义     

刘晨  孙景贵  古阿雷  赵克强  韩吉龙  杨梅  冯洋洋 《地质论评》2016,62(S1):37-38

大兴安岭地区是一个经历了古亚洲洋、蒙古-鄂霍茨克洋闭合和环太平洋构造体系叠加复合构造区,以其发育规模巨大、结构复杂的陆相火山岩带和花岗岩带以及有色、贵金属矿床备受国内外地质学家广泛关注和研究(Wu, et al.,2006;白令安,2013;Sun J G,2013;Xu,et al.,2013;苟军,2013)。目前,虽然该区在中生代陆相火山岩的成因、形成的构造背景已取得了较大进展;但是,由于大兴安岭地区火山作用复杂,自然地理、交通条件较差,尚有大面积空白区未开展过系统的1：50000区域地质矿产调查和科研工作;近期我们课题组在开展大兴安岭东坡花岗岩带北部1：50000小鄂尔贝尔汗（M51E012016）和小莫尔可（M51E011016）幅区域地质矿产调查过程中,将地质矿产调查与科研相结合,对侵入喷发在花岗岩岩体之间陆相火山岩进行了岩石地层厘定和岩相学、元素地球化学、年代学以及Hf同位素研究,以期为整体揭示大兴安岭火山岩的源区性质及其演化规律提供科学依据,并从成矿元素地球化学角度来约束成矿物质来源,并结合区域成矿作用探讨区域找矿意义。  相似文献