Purification of a Diatom and Its Identification to Cylindrotheca closterium     

WANG Song  ZHANG Lin  YANG Guanpin  ZHU Baohua  PAN Kehou 《中国海洋大学学报(英文版)》2015,(2):357-361

A diatom was purified with colony selection and continuous dilution methods. It was identified to Cylindrotheca closterium according to its morphological characteristics and rbc L and 18 s r RNA gene sequences. The alga was not sensitive to ampicillin and neomycin, but sensitive to chloramphenicol which inhibited its growth at concentrations ranging from 50 to 150 μg m L-1. The purified alga was easy to culture and its specific growth rate was 0.207 ± 0.002(d-1). It was resistant to pollution and could be harvested in an easy way. It was relatively high in lipid content(20.08% ± 0.67% of dry weight) and the combined amount of its 16:0 and 16:1(n-7), the most suitable resource of biodiesel, was as high as 64% of the total fatty acids, while the amount of polyunsaturated fatty acids reached 19.96%–20% of the total fatty acids. Thus the purified C. closterium can be cultured as a biodiesel producer or a nutrition supplement producer.  相似文献   

Evaluation of Cytotoxicity and Genotoxicity of Insecticide Carbaryl to Flounder Gill Cells and Its Teratogenicity to Zebrafish Embryos     

EY Manish Raj  GUO Huarong 《中国海洋大学学报(英文版)》2015,(2):362-374

In this study, we determined the cytotoxicity and genotoxicity of carbamate insecticide carbaryl to flounder gill(FG) cells and its teratogenicity to zebrafish embryos. The cytotoxicity of carbaryl to FG cells was determined with methods including MTT and neutral red uptaking(NRU), lactate dehydrogenase(LDH) releasing and Hoechst 33342 and propidium idodide(PI) double staining. Moderate cytotoxicity in a concentration-dependent manner was observed. The 24 h-IC50 value of 53.48 ± 1.21, 59.13 ± 1.19 and 46.21 ± 1.24 mg L-1 carbaryl was obtained through MTT, NRU and LDH assays, respectively. Double fluorescence staining demonstrated that carbaryl induced the death of FG cells mainly through necrosis. There was no significant genotoxicity found in the FG cells exposed to the highest testing concentration of carbaryl(20 mg L-1, P 0.05) as was demonstrated by Comet assay. Zebrafish embryos exposed to carbaryl at concentrations ≥10 mg L-1 displayed moderate toxic effects on the survival, spontaneous movement, hatching, heart rates of the embryos and their development, which were evidenced by yolk and pericardial sac edemas, body length reduction and tail flexure in time- and concentration-dependent manners at specific stages. The 24 h-, 48 h- and 96 h-LC50 values of carbaryl to zebrafish embryos were 41.80 ± 1.10, 17.80 ± 1.04 and 14.46 ± 1.05 mg L-1, respectively. These results suggested that carbaryl is moderately toxic to FG cells cultured in vitro and zebrafish embryos, and the FG cells were similar to zebrafish embryos in their sensitivity to carbaryl as 24 h-IC50 and LC50 indicated.  相似文献   

A Comparative Study of Fish Assemblages Near Aquaculture,Artificial and Natural Habitats   总被引：1，自引：0，他引：1  

WANG Zhenhua  CHEN Yong  ZHANG Shouyu  WANG Kai  ZHAO Jing  XU Qiang 《中国海洋大学学报(英文版)》2015,(1):149-160

Habitat plays a critical role in regulating fish community structure. Using the data collected from a monthly trammel net survey in Ma’an archipelago off the east coast of China, we evaluated impacts of five different habitats(artificial reefs, mussel farms, cage aquaculture, rocky reefs and soft bottom) on fish assemblages. This study suggests that artificial reefs(AR) have significantly higher species richness, abundance and diversity than mussel farms(MF) or soft bottom(SB) habitats during most seasons, and that fish taxa in the AR habitats are similar to those in the rocky reef(RR) habitats. Two different fish assemblage patterns were revealed in the study area using non-metric multidimensional scaling ordination: an assemblage dominated by reef fishes(especially by Scorpaenidae species) in AR, RR and cage aquaculture(CA) habitats and an assemblage dominated by Sciaenidae species in MF and SB habitats. We suggest that reef fishes play a key role in differentiating fish community structures in the study area. Although few differences in fish abundance and diversity were found between the CA and SB habitats, a more diverse age structure was observed in the CA habitats. A much more complex fish assemblage and enhanced population of local species were established as a result of the presence of both floating and fixed artificial structures, probably through improved survival rates.  相似文献   

Effect of Freeze-Thaw Cycles on Mechanical Properties and Permeability of Red Sandstone under Triaxial Compression   总被引：1，自引：1，他引：0  

YU Jin  CHEN Xu  LI Hong  ZHOU Jia-wen  CAI Yan-yan 《山地科学学报》2015,(1):218-231

Geological disasters will happen in cold regions because of the effects of freeze-thaw cycles on rocks or soils, so studying the effects of these cycles on the mechanical characteristics and permeability properties of rocks is very important. In this study,red sandstone samples were frozen and thawed with 0,4, 8 and 12 cycles, each cycle including 12 h of freezing and 12 h of thawing. The P-wave velocities of these samples were measured, and the mechanical properties and evolution of the steady-state permeabilities were investigated in a series of uniaxial and triaxial compression tests. Experimental results show that, with the increasing of cyclic freeze-thaw times, the P-wave velocity of the red sandstone decreases. The number of freeze-thaw cycles has a significant influence on the uniaxial compressive strength, elastic modulus, cohesion, and angle of internal friction. The evolution of permeability of the rock samples after cycles of freeze-thaw in a complete stress-strain process under triaxial compression is closely related to the variation of the microstructure in the rock. There is a highly corresponding relationship between volumetric strain and permeability with axial strain in all stages of the stress-strain behaviour.  相似文献   

Characteristics of convection and overshooting in RGB and AGB stars     

Xiang-Jun Lai    Yan Li  National Astronomical Observatories/ Yunnan Observatory  Chinese Academy of Sciences  Kunming  China  Key Laboratory for the Structure  Evolution of Celestial Objects  China Graduate University of Chinese Academy of Sciences  Beijing  China. 《中国天文和天体物理学报》2011,(10)

Based on the turbulent convection model (TCM) of Li & Yang, we have studied the characteristics of turbulent convection in the envelopes of 2 and 5M⊙ stars at the red giant branch and asymptotic giant branch phases. The TCM has been successfully applied over the entire convective envelopes, including the convective unstable zone and the overshooting regions. We find that the convective motions become progressively stronger when the stellar models are located farther up along the Hayashi line. In the convect...  相似文献   

Mutual eclipses of J2 Europa by J1 Io observed at Yunnan Observatory in 2009     

Xi-Liang Zhang  Zhong Liu National Astronomical Observatories/ Yunnan Observatory  Joint Laboratory for Optical Astronomy  Chinese Academy of Sciences  Kunming  China  Graduate University of Chinese Academy of Sciences  Beijing 《中国天文和天体物理学报》2011,(10)

Mutual events between natural satellites include mutual occultation and mutual eclipse. Mutual eclipse is another kind of mutual occultation as viewed from the center of the Sun instead of the Earth. Two mutual eclipses of J2 Europa by J1 Io (2009 Aug. 28 and Sept. 12) were observed at Yunnan Observatory during the PHEMU09 international campaign. We will calculate the astrometric data of these Galilean satellites by analyzing and fitting the light curves we obtained. The limb-darkening was considered during...  相似文献   

Solar sail time-optimal interplanetary transfer trajectory design     

Sheng-Ping Gong  Yun-Feng Gao  Jun-Feng Li School of Aerospace  Tsinghua University  Beijing  China 《中国天文和天体物理学报》2011,(8)

The fuel consumption associated with some interplanetary transfer trajectories using chemical propulsion is not affordable. A solar sail is a method of propulsion that does not consume fuel. Transfer time is one of the most pressing problems of solar sail transfer trajectory design. This paper investigates the time-optimal interplanetary transfer trajectories to a circular orbit of given inclination and radius. The optimal control law is derived from the principle of maximization. An indirect method is used...  相似文献   

A note on the discovery of a 2M_⊙ pulsar     

Xiao-Yu Lai  Ren-Xin Xu School of Physics  State Key Laboratory of Nuclear Physics  Technology  Peking University  Beijing  China 《中国天文和天体物理学报》2011,(6)

It is conventionally thought that the state equation of dense matter softens and thus cannot result in high maximum mass if pulsars are quark stars and that a recently discovered 2M⊙ pulsar (PSR J1614-2230) may make pulsars unlikely to be quark stars. However, this standard point of view would be revisited and updated if quark clustering could occur in cold quark matter because of the strong coupling be- tween quarks at realistic baryon densities in compact stars. It can be argued that the state equation of...  相似文献   

Cassini SAR, radiometry, scatterometry and altimetry observations of Titan’s dune fields     

A. Le Gall  M.A. Janssen  A.G. Hayes  C. Savage  R.D. Lorenz  R.L. Kirk  S. Wall  E.R. Stofan  the Cassini Radar Team 《Icarus》2011,213(2):608-624

Large expanses of linear dunes cover Titan’s equatorial regions. As the Cassini mission continues, more dune fields are becoming unveiled and examined by the microwave radar in all its modes of operation (SAR, radiometry, scatterometry, altimetry) and with an increasing variety of observational geometries. In this paper, we report on Cassini’s radar instrument observations of the dune fields mapped through May 2009 and present our key findings in terms of Titan’s geology and climate. We estimate that dune fields cover ∼12.5% of Titan’s surface, which corresponds to an area of ∼10 million km², roughly the area of the United States. If dune sand-sized particles are mainly composed of solid organics as suggested by VIMS observations (Cassini Visual and Infrared Mapping Spectrometer) and atmospheric modeling and supported by radiometry data, dune fields are the largest known organic reservoir on Titan. Dune regions are, with the exception of the polar lakes and seas, the least reflective and most emissive features on this moon. Interestingly, we also find a latitudinal dependence in the dune field microwave properties: up to a latitude of ∼11°, dune fields tend to become less emissive and brighter as one moves northward. Above ∼11° this trend is reversed. The microwave signatures of the dune regions are thought to be primarily controlled by the interdune proportion (relative to that of the dune), roughness and degree of sand cover. In agreement with radiometry and scatterometry observations, SAR images suggest that the fraction of interdunes increases northward up to a latitude of ∼14°. In general, scattering from the subsurface (volume scattering and surface scattering from buried interfaces) makes interdunal regions brighter than the dunes. The observed latitudinal trend may therefore also be partially caused by a gradual thinning of the interdunal sand cover or surrounding sand sheets to the north, thus allowing wave penetration in the underlying substrate. Altimetry measurements over dunes have highlighted a region located in the Fensal dune field (∼5° latitude) where the icy bedrock of Titan is likely exposed within smooth interdune areas. The hemispherical assymetry of dune field properties may point to a general reduction in the availability of sediments and/or an increase in the ground humidity toward the north, which could be related to Titan’s asymmetric seasonal polar insolation. Alternatively, it may indicate that either the wind pattern or the topography is less favorable for dune formation in Titan’s northern tropics.  相似文献   

Advanced Technology Solar Telescope: A status report     

S.L. Keil  T.R. Rimmele  J. Wagner  the ATST team 《Astronomische Nachrichten》2010,331(6):609-614

Magnetic fields control the inconstant Sun. The key to understanding solar variability and its direct impact on the Earth rests with understanding all aspects of these magnetic fields. The Advanced Technology Solar Telescope (ATST) has been design specifically for magnetic remote sensing. Its collecting area, spatial resolution, scattered light, polarization properties, and wavelength performance all insure ATST will be able to observe magnetic fields at all heights in the solar atmosphere from photosphere to corona. After several years of design efforts, ATST has been approved by the U.S. National Science Foundation to begin construction with a not to exceed cost cap of approximately $298M. Work packages for major telescope components will be released for bid over the next several months. An application for a building permit has been submitted (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)  相似文献