发光细菌法在环渤海污水样品遗传毒性检测中的应用     

崔志松  栾晓  高伟  李倩  杨官品  郑立 《海洋与湖沼》2015,46(1):228-232

建立了以经过遗传改造的发光细菌Acinetobacter sp.Rec A为受试物种的环境污染物遗传毒性快速检测方法,该方法最快可在3h内得到毒性评价结果。采用该方法评价了环渤海排污口12份污水样品的遗传毒性。环渤海12个排污口的污水样品均表现出了不同水平的遗传毒性,并呈现出一定的分布特征。其中,高毒水质集中出现在山东半岛污水样品中,中毒水质集中出现在辽东半岛地区,而低毒水质主要集中于京津冀地区。综上,在环渤海的污水样品遗传毒性检测中,这种新型发光细菌法具有快速、灵敏、简便等优点,可为以后近海环境的水质生物毒性检测提供参考依据,具有应用于近海环境水质快速监测与评价的潜力。  相似文献   

中国东海济舟岛南部春季水华的影响要素研究     

FU Dongyang  HUANG Zhaojun  ZHANG Yuanzhi  PAN Delu  DING Youzhu  LIU Dazhao  ZHANG Ying  MAO Zhihu  CHEN Jianfang 《海洋学报(英文版)》2015,34(3):51-58

A soil circulation occurs in the south of Cheju Island in the spring. Nutrients and its influence on chlorophyll a(Chl a) around the circulations were studied from April 9 to May 6, 2007. Spring bloom with elevated concentrations of Chl a was observed during the investigation. High concentrations of phosphate, nitrate and silicate at 0.6, 12, and 8 mmol/m3, respectively, were detected. A low water temperature prevented the growth of phytoplankton. Chl a concentrations in the study area might be strongly associated with the high silicate concentration.  相似文献   

西北冰洋楚科奇海台08P31孔晚第四纪的古海洋学记录     

梅静  王汝建  章陶亮  肖文申  陈志华  陈建芳  程振波  孙烨忱 《海洋学报》2015,37(5):121-135

本文通过中国第三次北极科学考察在北冰洋楚科奇海台采集的08P31孔样品的多项指标分析,重建该区晚第四纪的古海洋与古气候演变历史。将08P31孔沉积物记录与其他孔综合对比初步划分为氧同位素3期(MIS 3)-MIS 1的沉积序列;TOC与Opal含量分析结果显示,自MIS 3以来该区总生产力呈逐渐上升趋势,但硅质生产力不高;CaCO3含量不仅代表海生钙质生物的生产力,也是陆源碎屑碳酸盐岩的信号;根据δ13Corg值运用两端元法计算出TOCmar/TOC,并通过TOCmar、TOCter与C/N值比较可知,δ13Corg值作为有机质来源的指标更可靠。经分析该孔沉积物有机质以海源有机质为主,但褐色层B2与白色层W3的有机质主要受到陆源有机质输入的影响;该孔沉积物中的浮游有孔虫Neogloboquadrina pachyderma(sin.)(Nps)的δ18 O和-δ13 C结果显示:褐色层B2和灰色层G1的轻值是由冰融水造成;黄褐色层Yb1上部到白色层W3下部的轻值反映了海冰形成速率提高,导致轻同位素卤水的生产和下沉。黄褐色层Yb1中部及白色层W3,Nps-δ18 O和-δ13C的重值对应低的有孔虫丰度,指示水体温度下降以及低营养环境。黄褐色层Yb2的Nps-δ18 O和-δ13C值呈镜像关系,这是由于大冰期环境下,海盆内储存了较少的融冰水导致。  相似文献   

南海北部自由生活海洋线虫的群落结构和生物多样性     

刘晓收  许嫚  张敬怀  刘丹  李肖 《海洋学报(英文版)》2015,34(6):77-85

A quantitative study on the community structure and biodiversity of free-living marine nematodes and their relationship with environmental factors in the northern South China Sea were carried out based...  相似文献   

基于SODA数据集的南海海表面盐度分布特征与长期变化趋势分析     

陈海花  李洪平  何林洁  付文文 《海洋技术学报》2015,34(4):48-52

盐度是南海物理环境的重要组成部分之一,盐度的变化对南海的水动力环境有重要影响。利用1972-2010年的SODA数据集的5.01 m层月平均数据,分析南海海域海表面盐度的时空分布特征,采用基于最小二乘法的线性拟合分析南海海表面盐度的长期变化趋势。结果表明南海海域的海表面盐度在39 a间90%以上区域均呈现盐度下降趋势,整个海域SSS则以每年0.005 88 psu的速率下降,春季SSS下降速率最大为0.006 4 psu/a,夏季SSS下降速率在四季中最小为0.005 19 psu/a。  相似文献   

Experimental Study on Response Performance of VIV of A Flexible Riser with Helical Strakes   总被引：1，自引：1，他引：0  

高云  付世晓  曹静  陈一帆 《海洋工程》2015,29(5):673-690

Laboratory tests were conducted on a flexible riser with and without helical strakes. The aim of the present work is to further understand the response performance of the vortex induced vibration (VIV) for a riser with helical strakes. The experiment was accomplished in the towing tank and the relative current was simulated by towing a flexible riser in one direction. Based on the modal analysis method, the displacement responses can be obtained by the measured strain. The strakes with different heights are analyzed here, and the response parameters like strain response and displacement response are studied. The experimental results show that the in-line (IL) response is as important as the cross-flow (CF) response, however, many industrial analysis methods usually ignore the IL response due to VIV. The results also indicate that the response characteristics of a bare riser can be quite distinct from that of a riser with helical strakes, and the response performance depends on the geometry on the helical strakes closely. The fatigue damage is further discussed and the results show that the fatigue damage in the CF direction is of the same order as that in the IL direction for the bare riser. However, for the riser with helical strakes, the fatigue damage in the CF direction is much smaller than that in the IL direction.  相似文献   

十三陵地震台gPhone重力仪的仪器性能与水文响应分析   总被引：2，自引：0，他引：2  

佘雅文  付广裕  韦进 《大地测量与地球动力学》2015,35(5):901-905

对安置于十三陵地震台的两台gPhone重力仪(109、118号)在2013-04~08的连续观测数据进行潮汐分析和提取重力残差处理。结果表明,gPhone重力仪监测到降水导致μGal量级的重力变化;gPhone重力仪获取的二阶重力残差变化与距离十三陵地震台15km的沙河地震台静水位变化之间存在4d左右延迟的正相关对应关系,显示了地下水位观测对重力观测解释的必要性。因此,有必要对我国的连续重力观测网补充地下水位观测,提高连续重力观测的数据处理精度。  相似文献   

鲤JAK2基因分子克隆及组织表达分析     

付友  高谦  文春根  吴平  刘德立 《广东海洋大学学报》2015,(1):8-17

采用同源克隆策略和RACE-PCR技术,克隆得到可能的鲤(Cyprinus carpio)两面神激酶2(JAK2)基因的c DNA全长序列,包括3 378 bp的开放阅读框,732 bp的5'-非编码区,529 bp的3'-非编码区,总长度达4 639bp。开放阅读框可编码1 125个氨基酸,推测分子质量和理论等电点分别为129.33 ku和6.99。同源性分析显示,克隆的鲤鱼基因与斑马鱼(Danio rerio)JAK2同源性最高,其氨基酸序列同一性和相似度分别达89%和95%。结构域预测表明,所编码氨基酸序列包含FERM、SH2及两个酪氨酸激酶结构域,这4个结构域也保守地存在于其他脊椎动物的JAK分子中。高级结构预测显示,鲤JAK2主要包括α-螺旋(α-helix)、β-折叠(β-sheet)和连接环(loop)等3类结构元件。脊椎动物JAK分子系统进化树显示,JAK1、JAK2、JAK3和TYK2等4类JAK分子分别聚类,鲤JAK2处于JAK2支系中,与所有其他的鱼类JAK2聚为一大支,并与两栖类和哺乳类组成的另一大支构成姊妹群,表明它们具有共同的祖先基因,为直系同源关系。实时荧光定量PCR检测结果表明,鲤JAK2在皮肤中表达量最高,其次是肠、血液和脑,而在肌肉、鳃、头肾、心、肝、脾中表达量较低;鲤JAK3在脾中表达量最高,在其他组织中表达量均很低,甚至未检出。  相似文献   

青岛市黄岛区潮河水源地地下水数值模拟     

付佳妮  孙建明  林青 《山东国土资源》2015,31(7)

在分析研究青岛市黄岛区潮河流域水文地质条件基础上,建立了水文地质概念模型和地下水流数值模拟模型,利用地下水水位动态监测资料对模型进行了识别,并预测了三枯一丰一平条件下地下水最大开采量以及水位、降深分布情况。结果表明,模型验证拟合结果较好,地下水最大开采量为2.4万m3/d,地下水开采不会引起地面沉降、海水入侵等问题。  相似文献   

Seismic data reconstruction based on iterative linear expansion of thresholds     

LUO Teng  LIU Cai  WANG dian  YANG Xueting  FU Wei  ZHOU Yin  HE Mei 《世界地质(英文版)》2015,18(2)

Based on the compressive sensing,a novel algorithm is proposed to solve reconstruction problem under sparsity assumptions.Instead of estimating the reconstructed data through minimizing the objective function,the authors parameterize the problem as a linear combination of few elementary thresholding functions,which can be solved by calculating the linear weighting coefficients.It is to update the thresholding functions during the process of iteration.The advantage of this method is that the optimization problem only needs to be solved by calculating linear coefficients for each time.With the elementary thresholding functions satisfying certain constraints,a global convergence of the iterative algorithm is guaranteed.The synthetic and the field data results prove the effectiveness of the proposed algorithm.  相似文献