Diagnosis of iridovirus in large yellow croaker by PCR   总被引：1，自引：0，他引：1  

Chen Xinhu  Wang Xiaowen  Wu Wenzhong 《海洋学报(英文版)》2003,22(4):635-642

A rapid and sensitive PCR-based method for the detection of the large yellow croaker iridovirus(LYCIV) is described,which involves the amplification of a 295 bp fragment of the LYCIV ATPase gene from DNA isolated from naturally infected fish spleen.Sequencing of LYCIV ATPase gene fragment showed it shared 100% nucleotide sequence homology with the corresponding region of the ATPase gene of red sea bream iridovirus(RSIV) and sea bass iridovirus(SBIV),suggesting that LYCIV was homologous with RSIV and SBIV at least in part of the gemone.The specificity and sensitivity of the PCR procedure were tested on the iridovirus-infected fishes,the expected fragment was detected from spleen DNA samples of infected fishes,whereas no fragments were amplified from healthy fish spleen DNA,white spot syndrome baculoviruses(WSBV) DNA and pseudorabies virus(PRV) DNA.Detection limit of this method was 10^-7 ng positive plasmid DNA containing target sequence,equal to about 100 virions.In the infected experiment,first positive detection(1/4) appeared at Day 3 post-infection,all fish(4/4) tested positive at Day 7,however obvious symptoms were observed at Day 8,so LYCIV infection could be detected prior to the appearance of obvious symptoms.These results indicate that this PCR method could be used for early,rapid and specific detection of LYCIV infection.  相似文献   

水成沉积与风成沉积及古土壤的磁组构特征   总被引：14，自引：2，他引：14  

张家强  李从先  丛友滋 《海洋地质与第四纪地质》1999,19(2):85-94

晚第四纪水成沉积、风成沉积、古土壤等的磁组构研究表明,不同成因的沉积物具有明显不同的磁组构特征。一般地,水成沉积的体积磁化率Ｋ受物源和水动力因素控制,Ｐ和Ｆ均＞１．０２,ｑ＜０．５;风成沉积的Ｋ受物源控制,内陆黄土的Ｋ明显高于沿海黄土及海岸风沙Ｋ,Ｐ和Ｆ均＜１．０２,ｑ的平均值＞０．５５;古土壤的Ｋ受母质沉积类型和成土环境的氧化还原条件控制,内陆古土壤的Ｋ偏高,沿海古土壤的Ｋ偏低,内陆古土壤的Ｋ远高于沿海古土壤的Ｋ,Ｐ和Ｆ均＜１．０２,ｑ的平均值在０．５左右。运用典型沉积的磁组构特征对比分析未知沉积物,可以得到未知沉积物的物源、沉积过程和环境信息。  相似文献   

小球藻紫外线诱变及高产藻株筛选   总被引：1，自引：0，他引：1  

张学成  时艳侠  孟振 《中国海洋大学学报(自然科学版)》2007,37(5):749-753

利用单细胞分离技术和紫外诱变育种技术,筛选高产小球藻株。通过单细胞分离技术获得的藻株Chlorella vul-garisQ7,生长速度和蛋白含量均优于其它藻株。以C.vulgarisQ7为出发株,进行紫外诱变,从120株诱变单克隆中筛选出3个株系:M51,M59,M73。与出发株相比,突变株M51,M59,M73的生长速率分别提高了6.23%,3.8%,5.92%。蛋白含量分别提高了2.5%,3.1%,1.9%。研究了诱变株的最适生长条件,结果表明在140μmol.m-2.s-1,25℃时,诱变株具有最大的生长速率和生物量。探讨了诱变株的遗传稳定性,结果表明诱变株可稳定遗传。  相似文献   

雌雄比对海带雌配子体发育的影响     

罗世菊  丛沂周  杨迎霞  张全胜  李晓捷  朱龙  张壮志 《海洋湖沼通报》2005,(1):33-36

本实验进行了不同雌雄比对海带雌配子体发育的影响。结果表明：雌雄比(鲜重)为1：1及2：1，实验组出现排卵时间最早，且发育率高，实验第20天，发育率超过70％，孢子体正常。5：1，10：1，20：1实验组随着雄性比例的减少，发育率逐渐降低，随着培养天数的增加，三个实验组均出现未受精卵及畸形孢子体，雌雄比越大，未受精卵数目越多。  相似文献   

In-Situ Observation of Fluid Mud in the North Passage of Yangtze Estuary, China   总被引：1，自引：0，他引：1  

李九发  万新宁何青应铭  时连强S.M.Hutchinson 《中国海洋工程》2004,18(1):149-156

Observations of fluid mud were made in the lower North Passage of the Yangtze Estuary in February 2000, on 10 -11 August 2000, on 30 - 31 August 2000 (after two strong typhoons), on 21 - 24 August 2000 (neap tide) and on 3 -6 September 2000 (mean tide) respectively. In situ data show that the fluid mud in this area consists of fine cohesive sediment (median size 7.23 μm). The formation and movement of fluid mud varied during the neap-spring and flood-ebb tidal cycle. Observations suggest that fluid mud phenomena in this area may be categorised in a three-fold manner as slack water, storm and saltwedge features. The thickness of the fluid mud layer of slack water during the neap tide ranged from 0.2 to 0.96 m, whereas during the mean tide, the thickness ranged from 0.17 to 0.73 m, and the thickness of the fluid mud layer was larger during slack water than at the flood peak. Shoals cover an area of 800 km^2 with a water depth smaller than 5 m. Erosion of these extensive intertidal mudflats due to storm action provides an abundant sediment source. This is particularly significant in this estuary when the tidal level is lower than 5 m. The lower North Passage is a typical zone of saltwater wedging, so the saltwedge fluid mud has the most extensive spatial range in the estuary.  相似文献   

黄河三角洲埕岛海域浅地层剖面结构与灾害地质     

李俊杰  李广雪  文世鹏  杨荣民  郝坤 《海洋地质前沿》2007,23(12):8-13

通过钻孔和高分辨率浅地层剖面的综合对比,对黄河三角洲北部与埕岛油田海底工程密切相关的浅地层剖面结构进行细分,划分出末次冰期低海面以来的5套沉积相层和6个地震相层.分析表明,本区广泛分布的地质灾害是由其特殊的沉积结构和复杂的动力环境造成的,尤其是工程软弱层的存在造成三角洲前缘的不稳定性.  相似文献   

南澎列岛及邻近海域地质地貌与灾害地质分析   总被引：2，自引：1，他引：2  

詹文欢  张乔民  孙宗勋  唐诚  丘学林 《热带海洋学报》2002,21(1):11-17

南澎列岛及邻近海域是广东沿海灾害地质因素较集中和类型较齐全的地区之一。根据野外调查资料，简要地分析了区内地质地貌以及活动断裂、地震活动、地面沉降、滑坡、地裂和沙土液化等基本特征和分布规律，同时还对区内地质灾害进行了初步分析。  相似文献   

南沙群岛海区珊瑚礁灾害性地质分析   总被引：3，自引：0，他引：3  

詹文欢  孙宗勋  张乔民  唐诚 《热带海洋学报》2002,21(2):58-65

珊瑚礁是一种特殊的岩土介质，有独特的灾害地质类型。珊瑚礁灾害地质研究是现代工程地质学的一个新课题。通过珊瑚礁的地质记录研究了内动力灾害地质类型（包括活动断裂、地震和火山活动等）、外动力灾害地质类型（包括海底槽谷与地形突变带、崩塌、滑坡、峰礁与埋藏礁、泥丘、埋藏负地貌等），同时还分析了人类活动对珊瑚礁发育的影响。  相似文献   

双壳类软体动物精子发生及其在系统演化研究中的应用前景   总被引：11，自引：0，他引：11  

竺俊全  杨万喜 《海洋湖沼通报》2002,(4):25-31

综述了双壳类软体动物精子发生及精子形态结构方面的研究进展，指出双壳类软体动物精子发生过程中精细胞分化具有种、属特异性，精细胞分化的细节是区别个体外部形态很近的物种的依据；精子的形态结构具有种的特征，精子结构的比较研究将成为双壳类软体动物系统演化研究的重要方法之一。  相似文献   

Sediment transport pattern at the Barra Nova inlet, south Portugal: a conceptual model     

Yann Balouin  Hélène Howa 《Geo-Marine Letters》2002,21(4):226-235

The Barra Nova inlet, in south Portugal, is known to migrate progressively southeastwards under wave action. The morphodynamics of this system during a representative year suggests that this long-term evolution is dependent on a seasonal behavior of the tidal inlet which can be described through a three-stage model of post-storm, transition and extended fair-weather conditions. Processes involved in this evolution indicate that the historical migration of the Barra Nova is not dependent on the longshore drift forcing constricting the channel on the updrift coast, but rather on the adjustment of the system to a major erosion of the downdrift coast during short storm events.  相似文献