凡纳滨对虾咸淡水养殖系统内细菌群落组成的PCR-DGGE分析   总被引：16，自引：0，他引：16  

罗鹏  胡超群  谢珍玉  张吕平  任春华  许尤厚 《热带海洋学报》2006,25(2):49-53

采用PCR(polym erase chain reaction)-DGGE(denaturing grad ient gel electrophoresis)及传统的微生物培养方法对凡纳滨对虾Litopenaeus vannam ei咸淡水养殖系统内各种环境的细菌群落组成进行了比较研究。传统的微生物培养计数表明,从海湾水、养殖池水到对虾粪样,细菌和弧菌的数量表现出依次增加的趋势,粪样及肠壁中弧菌的数量高出外界水环境1—4个数量级。相对于外界水环境,粪样中有很高的芽孢杆菌孢子含量,但是肠壁定植细菌中不存在芽孢杆菌(孢子)。PCR-DGGE及聚类分析结果表明,从海湾沿岸、养殖池、对虾粪便到对虾肠壁,细菌群落的多样性由高到低,无论是在哪种环境,群落的优势种都十分明显,且只有2—4种。来自同一环境各样品间的细菌群落组成非常相似,来自不同环境的样品,其细菌群落组成差别较大。聚类图上各簇的排列顺序反映了样品在取样空间分布上的毗邻次序和它们的相似程度。  相似文献   

Comments on the Gravity and Magnetic Anomalies of Saros Bay using Wavelet Approach     

Ucan  Osman N.  Albora  A. Muhittin  Hisarli  Z. Mümtaz 《Marine Geophysical Researches》2001,22(4):251-264

In this paper, residual and regional gravity and magnetic anomalies of Saros Bay are separated using wavelet method. Wavelet is one of the modern, stochastic image approaches processing technique in electronics. The vertical, horizontal and diagonal components of wavelet output are evaluated simultaneously and an underground model is obtained by suitable cross-sections. Thus the geological and tectonic properties of Saros Bay are extracted. Our proposed model is confirmed by deep and shallow seismic researches of Turkish Petroleum Cooperation (TPAO). The South-East region of Saros Graben is formed by strike slip Ganos Fault and Anafartalar reverse Fault that lies on the east of Ganos Fault. On the Northwest region, there is strike slip component fault resulting in the Enez Graben. Here we detected two new oval type geological structure, both of them starting from the Eastern direction of Canakkale Dardanelles and but one of them continuing through Gelibolu Peninsuls, while the other tending to the Enez Graben. We think that these structures are the ruins of a very old sea in this region. This revised version was published online in November 2006 with corrections to the Cover Date.  相似文献   

An improved Ewing heat probe frame   总被引：3，自引：0，他引：3  

Tony F. Clark  Frank J. Malcolm  Ben J. Korgen 《Marine Geophysical Researches》1972,1(4):451-455

The standard Ewing heat probe frame has been improved through the design of a new takedown model whose parts are held together by lynch pins. When a 600 lb (272 kg) unit of this type is taken apart, the heaviest single piece of the dismantled unit weighs only 75 lb (34 kg).This makes it feasible for one person to handle, transport, and assemble the new unit. A central, main support member of a heat probe using this frame can be suspended over the side of a research vessel while the heavier temperature recorder and ballast weights are attached by means of the quickaction lynch pins. This makes it possible to use this device on relatively small vessels which are not designed for coring work, having a minimum of free deck space and without launching platforms or hydraulic A-frame booms.  相似文献   

A global heat and freshwater forcing dataset for ocean models     

Frank Rske 《Ocean Modelling》2006,11(3-4):235-297

A global dataset based on the ECMWF Re-Analyses (ERA) is presented that can be used as surface boundary conditions for ocean models with sea-ice components. The definition of these conditions is based on bulk formulae. To study the mean ocean circulation, a mean annual cycle on a daily basis was constructed from ERA for all relevant parameters including wind stress. Continental runoff is considered by using information about the catchment areas of the rivers and about the main drainage basins. The bulk formulae were extended by using sea ice concentration.To estimate meridional heat transports (MHT) and to avoid any drift in ocean model simulations, the heat and fresh water budgets have been closed by applying an inverse procedure to fine-tune the fluxes towards observed transports. To improve the MHTs on the Southern Hemisphere the winds and the short wave radiation at southern higher latitudes should be corrected. Furthermore, tests were performed concerning short wave radiation which was increased in the tropics and decreased in the subsidence zones.The heat and fresh water fluxes are assessed by using a scheme of Macdonald and Wunsch based on hydrographic sections. The net heat fluxes of ERA and of the forcing dataset are consistent with the heat flux divergences and convergences estimated by this scheme except for parts of the South Atlantic and the Indian Ocean sector of the Southern Ocean where none of these datasets is consistent with these estimates. In the subtropical South Indian Ocean the forcing dataset is consistent with these estimates while ERA are not. The flux components of ERA and the forcing dataset were compared to several observational datasets (SRB, SOC, HOAPS, GPCP, and CMAP). For each component, at least one of these datasets (especially HOAPS) supports the effects of the inverse procedure and the bulk formulae almost globally with some regional exceptions: short wave radiation in the tropical oceans and the subtropical North Atlantic, latent heat flux at higher latitudes, and precipitation in the northern North Atlantic.Comparisons to the NCAR/NCEP Re-Analyses (NRA) (versions 1 and 2) and the ECHAM model in place of ERA lead to similar results. In the North Atlantic the net heat fluxes of the model based datasets approach the hydrographic estimate with increasing resolution. Applied to any ocean/sea-ice model and compared to ERA, the forcing dataset would induce only a relative small net sea-surface buoyancy loss.A comparison of the forcing dataset to measurements made using one buoy deployed in the western Pacific warm pool and five buoys deployed in the subduction region of the Northeast Atlantic shows that at the site of the first buoy the net heat fluxes of the forcing dataset are in poorer agreement than those of ERA. At the sites of two subduction buoys both datasets show the same level of agreement within the error bars specified. At the sites of the three remaining subduction buoys the forcing dataset shows a marginal improvement on ERA.  相似文献   

Adaptive fisheries management in Vietnam: The use of indicators and the introduction of a multi-disciplinary Marine Fisheries Specialist Team to support implementation     

Jesper Raakjær  Dao Manh Son  Karl-Johan Stæhr  Holger Hovgård  Nguyen Thi Dieu Thuy  Kristine Ellegaard  Frank Riget  Dang Van Thi  Phung Giang Hai 《Marine Policy》2007

Vietnam is in the process of introducing adaptive management based on indicators and time-series to manage its marine fisheries. The development of indicator-based frameworks for fisheries management has accelerated in recent years. This article describes the suite of indicators being suggested or used in Vietnam including the data-collection system. The Vietnamese approach is supported by an institutional structure consisting of a multi-disciplinary advisory system to facilitate the process of using indicators and to introduce knowledge-based management. The article concludes by identifying current problems and weaknesses and by providing suggestions for future improvement of the system.  相似文献   

An evaluation of the Emerald/Western Bank juvenile haddock closed area     

Frank  Kenneth T.; Shackell  Nancy L.; Simon  James E. 《ICES Journal of Marine Science》2000,57(4):1023-1034

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Meadow fragmentation and reproductive output of the SE Asian seagrass Enhalus acoroides     

Jan E. Vermaat  Rene N. Rollon  Cristina Day A. Lacap  Claire Billot  Filipe Alberto  Hildie M.E. Nacorda  Frank Wiegman  Jorge Terrados 《Journal of Sea Research》2004,52(4):201

Flower and fruit production of the abundant, tall, long-lived, dioecious, surface-pollinating seagrass species Enhalus acoroides (L.) Royle were estimated at seven sites in the reef flats off Bolinao (NW Luzon, The Philippines) featuring different fragmentation of the seagrass meadows. Fragmentation of the seagrass meadow was quantified as cover of E. acoroides and all seagrass species present in 20×20 m plots. E. acoroides and overall seagrass cover were correlated positively. The proportion of female flowers of E. acoroides that developed a fruit increased sharply as overall seagrass cover was around 50%. Apparent sex ratio bore no relationship with overall seagrass cover. This threshold-type of relationship suggests that fragmentation of seagrass meadows can have a major effect on the reproductive output of this species. A possible mechanism underlying these results would be a non-linear increase of the efficiency of trapping the surface-dispersed pollen with increasing seagrass canopy density. This provides the first evidence based on real data that fragmentation can affect the population dynamics of seagrass species.  相似文献   

On the longitudinal distribution of the solar surges     

Atίla Özgüç 《Solar physics》1989,123(2):381-383

After re-examination of data presented by Verma (1984), some misuse of solar parameteres published in Solar Geophysical Data is presented. Consequently some conclusions made by Verma (1984, 1986) on about the longitudinal distribution of cool solar surges based on these misuses are discussed.  相似文献   

The GeoForschungsZentrum Potsdam/Groupe de Recherche de Gèodésie Spatiale satellite-only and combined gravity field models: EIGEN-GL04S1 and EIGEN-GL04C   总被引：2，自引：1，他引：1  

Christoph Förste  Roland Schmidt  Richard Stubenvoll  Frank Flechtner  Ulrich Meyer  Rolf König  Hans Neumayer  Richard Biancale  Jean-Michel Lemoine  Sean Bruinsma  Sylvain Loyer  Franz Barthelmes  Saskia Esselborn 《Journal of Geodesy》2008,82(6):331-346

The recent improvements in the Gravity Recovery And Climate Experiment (GRACE) tracking data processing at GeoForschungsZentrum Potsdam (GFZ) and Groupe de Recherche de Géodésie Spatiale (GRGS) Toulouse, the availability of newer surface gravity data sets in the Arctic, Antarctica and North-America, and the availability of a new mean sea surface height model from altimetry processing at GFZ gave rise to the generation of two new global gravity field models. The first, EIGEN-GL04S1, a satellite-only model complete to degree and order 150 in terms of spherical harmonics, was derived by combination of the latest GFZ Potsdam GRACE-only (EIGEN-GRACE04S) and GRGS Toulouse GRACE/LAGEOS (EIGEN-GL04S) mean field solutions. The second, EIGEN-GL04S1 was combined with surface gravity data from altimetry over the oceans and gravimetry over the continents to derive a new high-resolution global gravity field model called EIGEN-GL04C. This model is complete to degree and order 360 and thus resolves geoid and gravity anomalies at half- wavelengths of 55 km at the equator. A degree-dependent combination method has been applied in order to preserve the high accuracy from the GRACE satellite data in the lower frequency band of the geopotential and to form a smooth transition to the high-frequency information coming from the surface data. Compared to pre-CHAMP global high-resolution models, the accuracy was improved at a spatial resolution of 200 km (half-wavelength) by one order of magnitude to 3 cm in terms of geoid heights. The accuracy of this model (i.e. the commission error) at its full spatial resolution is estimated to be 15 cm. The model shows a reduced artificial meridional striping and an increased correlation of EIGEN-GL04C-derived geostrophic meridional currents with World Ocean Atlas 2001 (WOA01) data. These improvements have led to select EIGEN-GL04C for JASON-1 satellite altimeter data reprocessing. Electronic Supplementary Material The online version of this article (doi:) contains supplementary material, which is available to authorized users.  相似文献   

Efficient GOCE satellite gravity field recovery based on least-squares using QR decomposition   总被引：3，自引：0，他引：3  

Oliver Baur  Gerrit Austen  Jürgen Kusche 《Journal of Geodesy》2008,82(4-5):207-221

We develop and apply an efficient strategy for Earth gravity field recovery from satellite gravity gradiometry data. Our approach is based upon the Paige-Saunders iterative least-squares method using QR decomposition (LSQR). We modify the original algorithm for space-geodetic applications: firstly, we investigate how convergence can be accelerated by means of both subspace and block-diagonal preconditioning. The efficiency of the latter dominates if the design matrix exhibits block-dominant structure. Secondly, we address Tikhonov-Phillips regularization in general. Thirdly, we demonstrate an effective implementation of the algorithm in a high-performance computing environment. In this context, an important issue is to avoid the twofold computation of the design matrix in each iteration. The computational platform is a 64-processor shared-memory supercomputer. The runtime results prove the successful parallelization of the LSQR solver. The numerical examples are chosen in view of the forthcoming satellite mission GOCE (Gravity field and steady-state Ocean Circulation Explorer). The closed-loop scenario covers 1 month of simulated data with 5 s sampling. We focus exclusively on the analysis of radial components of satellite accelerations and gravity gradients. Our extensions to the basic algorithm enable the method to be competitive with well-established inversion strategies in satellite geodesy, such as conjugate gradient methods or the brute-force approach. In its current development stage, the LSQR method appears ready to deal with real-data applications.  相似文献