Photoinduced toxicity single and binary mixtures of four polycyclic aromatic hydrocarbons to the marine diatom Skeletonema costatum   总被引：1，自引：0，他引：1  

MENG Wei  WANG Liping  ZHENG Binghui 《海洋学报(英文版)》2007,26(6):41-50

Diatom Skeletonema costatum Cleve is one of the main predominant phytoplankton species in the Changjiang Estuary in China. In order to provide some basic information for future assessment of the potential risk on phytoplankton communities in this estuary caused by polycyclic aromatic hydrocarbons(PAHs), this alga was selected as a representative to investigate the photoinduced toxicity of PAHs, in single and mixture. Four PAHs including three-ring phenanthrene and anthracene, four-ring fluoranthene and pyrene were tested in the laboratory. The single toxicity of each PAH on this microalga was compared with and without the simulated solar UV radiation. The results showed that this microalga was sensitive to PAH's photoinduced toxicity. Ratios of the 72 h median effect concentration obtained for fluorescent and UV light tests were about 8.4 for phenanthrene, 13.0 for anthracene, 6.5 for fluoranthene, and 5.7 for pyrene, indicating that UV light enhanced the PAH toxicity to this alga significantly. Under the fluorescent radiation (lacking UV), the dose-response curves based on chemical concentrations revealed that the order of toxic strength was fluoranthene greater than pyrene greater than anthracene greater than phenanthrene; while under the UV radiation (476 μW/cm2 for UVA, 6.5 μW/cm2 for UVB) it became fluoranthene approximately equaling anthracene greater than pyrene greater than phenanthrene, indicating that the UV light also changed its relative toxicity to this alga. The photoinduced toxicity of PAHs to the marine diatom S. costatum might be a synergistic effect of photosensitization reactions (e.g., generation of single-state oxygen) and photomodification (photooxidation and/or photolysis).The combined effects of six binary mixtures on the marine diatom S. costatum were investigated using the additive-index method. Four binary-mixtures (phenanthrene plus anthracene; phenanthrene plus pyrene; anthracene plus fluoranthene; anthracene plus pyrene) were found to be synergistic joint action mode, while two binary-mixtures (phenanthrene plus fluoranthene; fluoranthene plus pyrene) displayed antagonist, revealing a complex pattern of possible interactions. The combined action mode of PAHs might be related to various factors such as the mixture compounds, mixture ratios and test conditions, etc.  相似文献   

固定化鲅鱼乙酰胆碱酯酶的制备及部分性质测定   总被引：3，自引：0，他引：3  

孟范平  何东海  朱小山  马冬冬  杨正先  YANG Zheng-Xian 《中国海洋大学学报(自然科学版)》2005,35(6):1067-1071

酶的固定化是酶传感器制备过程中重要的1个环节.研究采用直接共价法固定鲅鱼乙酰胆碱酯酶（AChE）.制备方法为：0.1g CNBr活化的Sepharose 4B凝胶用1mmol/L的HCl充分溶胀后,与活力为10U的AChE溶液混合,于4℃下150r/min振荡8h.所制备的固定化酶活力回收率较高（96%）,对pH值和温度变化的适应能力均优于非固定化酶;在3个月保存期内,前者的活力损失13%,而后者的活力则下降89%.这说明固定过程能够大大提高AChE的抗逆性和保持酶活力的稳定,有利于酶传感器的制备.  相似文献   

南海东北部表层沉积中生源和矿物碎屑组分分析及其古环境意义   总被引：9，自引：3，他引：9  

孟翊  严肃庄  陈荣华  张富元 《海洋地质与第四纪地质》2001,21(3):17-22

通过对南海东北部128个表层沉积样品的定量研究，揭示隆源和矿物碎屑各组分的分布，也显示了组分分析作为一种简单、快速的研究方法在海洋古环境研究中的潜力，研究区生源碎屑的分布明显受与水深相关的深海溶解作用所控制，水深3500-4000m的现代南海CCD以下，钙质生物的含量急剧减少，而硅质生物的含量急剧增加，研究区表层沉积中的碎屑矿物主要来源于亚洲大陆，吕宋镐的风化产物只起次要作用，且其分布主要受与离岸距离远近和海流相关的搬运作用所控制。  相似文献   

PLS-ANN分析血浆自体荧光光谱的二阶导数光谱识别胃癌   总被引：1，自引：0，他引：1  

马君  朱玉平  毛伟征  李颖  史晓凤  郑荣儿  孟继武 《中国海洋大学学报(自然科学版)》2006,36(3):505-508

探讨偏最小二乘法结合神经网络法(简称PLS-ANN)分析血浆自体荧光光谱的二阶导数光谱识别胃癌的优势,对20例胃癌病人和23例健康人血浆进行以405nm为激发光的自体荧光光谱检测,采用PLS-ANN法分别对血浆自体荧光光谱和二阶导数光谱进行判别分析。PLS-ANN分析血浆的自体荧光光谱法诊断胃癌的灵敏度为75%,特异度为83%,准确率为79%,PLS-ANN分析血浆的二阶导数光谱法诊断胃癌的灵敏度为90%,特异度为96%,准确率为93%。结果表明PLS-ANN分析血浆的二阶导数光谱法识别胃癌,优于PLS-ANN分析血浆的自体荧光光谱法,有望成为快速识别胃癌的较理想方法。  相似文献   

Refraction-Diffraction Mathematical Model for Waves With Lateral Energy Transmission Mechanism and Its Verification     

Xu Shikai  Wang Hongchuan Hong Guangwen Engineer  Nanjing Hydraulic Research Institute  Nanjing  . Professor  Research Institute of Coastal  Ocean Engineering  Hohai University  Nanjing 《中国海洋工程》1996,(4)

To solve problems concerning wave elements and wave propagation, an effective way is the wave energy balance equation, which is widely applied in oceanography and ocean dynamics for its simple computation. The present papaer advances wave energy balance equations considering lateral energy transmission and energy loss as the governing equation for the study of wave refraction-diffraction. For the mathematical model, numerical simulation is made by means of difference method, and the result is verified with two examples.  相似文献   

A Preliminary Study of Quantitative Distribution of Benthic Animals in the East China Sea Continental Shelf and Its Adiacent Waters     

江锦祥  黄立强  孟凡  吴启泉  王慧珍  孙修勤 《海洋学报(英文版)》1985,4(4):648-649

In October and November of 1977 and September and October of 1978,a quantitative survey of benthic animals was conducted in the East China Sea Continental Shelf and its adjacent waters(26°00'-34°00'N,124°00'-129°00'E,water depth 39-1550m),where 307 stations were dredged and 1200 samples collected.  相似文献   

辽东湾S2冰侧限剪切强度的试验研究   总被引：3，自引：0，他引：3  

李志军孟广琳  高树刚刘培林 《海洋工程》2002,20(1):20-23

利用试验方法系统地研究了辽东湾S2型海冰侧限剪切强度随剪切应变速率、加载方向、法向应力、温度和冰内孔隙率的变化规律。介绍了S2型海冰各向异性特点及剪切应变速率对剪切破坏方式的效应和韧脆破坏转变的条件。分析了不同加载方向海冰峰值剪切强度的差异 ,获得 - 6 2℃温度下S2冰内摩擦角和内聚力分别为 42°和 70 0kPa。利用统计分析给出峰值剪切强度与冰温和孔隙率的试验关系  相似文献   

浪流作用下系泊船舶撞击力和系缆力试验研究     

李焱  郑宝友  高峰  孟祥玮 《海洋工程》2007,25(2):57-63

以某25万吨矿石码头工程为例,分别进行了单流、单浪和浪流共同作用下,系泊船舶撞击力和系缆力试验。研究了不同水位、不同船舶载度、不同浪流夹角,单流、单浪和浪流共同作用时对船舶撞击力和系缆力的影响。分析了该码头工程护舷和缆绳的布置情况,为工程设计提供了依据。  相似文献   

东海北部黑潮区浮游动物的多样性研究   总被引：5，自引：1，他引：5  

洪旭光  张锡烈  俞建銮  孟凡 《海洋学报》2001,23(1):139-142

由于浮游动物在海洋生态系统中处于主导地位及其对生物地球化学循环和全球变化的重要影响,浮游动物与环境间相互作用的研究已成为现代海洋生态学研究的一个核心问题[1].  相似文献   

神舟四号高度计波形数据预处理和信息提取   总被引：1，自引：0，他引：1  

纪永刚  张杰  张有广  孟俊敏 《海洋与湖沼》2007,38(6):487-494

神舟四号(SZ-4)高度计在国内首次提供了星载雷达高度计回波波形数据.本文中作者分析了SZ-4高度计回波波形的特点,完成波形数据的预处理,并在此基础上完成初步的信息提取.在数据预处理方面,通过SZ-4高度计水陆边界处波形的特点,提出了波形最大幅度控制的方法,筛选回波波形.在波形归一化处理过程中,发现SZ-4高度计波形中存在双峰现象,并指出第二个峰为异常波形区.在波形信息提取方面,利用波形重新跟踪得到的半功率点计算出SZ-4高度计高度跟踪补偿误差,并根据高度计天线指向角和回波波形下降沿斜率之间的关系,从波形后沿提取天线指向角信息.分析结果表明,SZ-4高度计天线指向比较平稳,而跟踪补偿由于变化较大,在计算海面高度时,应作为一项误差源被考虑到.  相似文献