The scavenging of free radical and oxygen species activities and hydration capacity of collagen hydrolysates from walleye pollock (Theragra chalcogramma) skin     

Yongliang Zhuang  Bafang Li  Xue Zhao 《中国海洋大学学报(英文版)》2009,8(2):171-176

Fish skin collagen hydrolysates (FSCH) were prepared from walleye pollock (Theragra chalcogramma) using a mixture of enzymes, namely trypsin and flavourzyme. The degree of hydrolysis of the skin collagen was 27.3%. FSCH was mainly composed of low-molecular-weight peptides and the relative proportion of <1000Da fraction was 70.6%. Free radical and oxygen species scavenging activities of FSCH were investigated in four model systems, including diphenylpicrylhy-drazyl radical (DPPH), superoxide anion radical, hydroxyl radical and hydrogen peroxide model, and compared with that of a native antioxidant, reduced glutathione (GSH). FSCH was also evaluated by water-absorbing and water-holding capacity. The results showed that FSCH was able to scavenge free radical and oxygen species significantly and to enhance water-absorbing and water-holding capacity remarkably. Therefore, FSCH may have potential applications in the medicine and food industries.  相似文献   

Screening of extraction methods for glycoproteins from jellyfish (Rhopilema esculentum) oral-arms by high performance liquid chromatography     

Guoyan Ren  Bafang Li  Xue Zhao  Yongliang Zhuang  Mingyan Yan  Hu Hou  Xiukun Zhang  Li Chen 《中国海洋大学学报(英文版)》2009,8(1):83-88

In order to select an optimum extraction method for the target glycoprotein (TGP) from jellyfish (Rhopilema esculentum) oral-arms, a high performance liquid chromatography (HPLC)-assay for the determination of the TGP was developed. Purified target glycoprotein was taken as a standard glycoprotein. The results showed that the calibration curves for peak area plotted against concentration for TGP were linear (r = 0.9984, y = 4.5895x+47.601) over concentrations ranging from 50 to 400 mgL-1. The mean extraction recovery was 97.84% (CV2.60%). The fractions containing TGP were isolated from jellyfish (R. esculentum) oral-arms by four extraction methods: 1) water extraction (WE), 2) phosphate buffer solution (PBS) extraction (PE), 3) ultrasound-assisted water extraction (UA-WE), 4) ultrasound-assisted PBS extraction (UA-PE). The lyophilized extract was dissolved in Milli-Q water and analyzed directly on a short TSK-GEL G4000PWXL (7.8 mm×300 mm) column. Our results indicated that the UA-PE method was the optimum extraction method selected by HPLC.  相似文献   

Study of Panjin wetlands along Bohai coast (II): Ecological water requirement of Shuangtaizi estuarine wetland     

Tieliang Wang  Linfei Zhou  Bo Zhao  Peiqi Yang 《中国海洋大学学报(英文版)》2009,8(2):197-202

Shuangtaizi estuarine wetland along the Bohai Sea coast, the biggest bulrush wetland in the world, has been listed in ‘The Record of Important International Wetland Conservation District’. Taking the year of 2 000 as an example, the minimum, the most suitable and the maximum ecological water requirement of Shuangtaizi estuarine wetland are calculated in this paper based on both ecological theory and Geological Information System technology. In addition, the remote sensing technique is adopted in the data acquisition process. Moreover, the total water requirement and the unit area water requirement for different wetland types are obtained. The result is very important for water resources planning, ecological conservation and regional agriculture structure adjustment in Shuangtaizi. Meanwhile, this study can serve as a useful example for calculating the ecological water requirement in other similar estuarine wetlands.  相似文献   

东营市矿产资源开发利用现状与保护措施          下载免费PDF全文

巴文庄  王峰  徐倩  王辉丽  袁峥  齐成彦 《山东国土资源》2009,25(4)

以东营市矿产资源总体规划为指导,进行矿产资源开发利用与保护措施研究,在开发利用现状的基础上分析当前存在的矿产资源后备基地紧缺、综合利用水平不高以及过度开采等问题,进而提出促进矿产资源开发利用与保护的措施,以便更好地为东营市经济建设和社会发展服务。  相似文献   

工程物探方法在地质灾害评估中的应用     

王仕昌  丁荣胜  赵兰春 《山东国土资源》2009,25(10):45-47

利用工程物探方法对济南市历城区全民健身中心建设项目铁矿采空区进行了勘查工作,结果表明物探电阻率测深法勘查工作,对铁矿采空区反映效果明显,结合磁测资料与现场调查访问,推测出评估区内的2处隐伏采空区影响范围基本与事实一致。  相似文献   

海洋主体功能区划与海洋功能区划关系研究     

王倩  郭佩芳 《海洋湖沼通报》2009,(4)

开展海洋主体功能区划与海洋功能区划的关系和衔接研究,是保障两者在我国海洋事业中充分发挥各自作用的基础.本文从编制及实施2个方面理顺海洋主体功能区划与海洋功能区划的关系,促进两者在实际工作中的相互结合.  相似文献   

松南十屋断陷深层油气成藏条件与模式研究     

赵勇刚  张树林 《海洋地质译丛》2009,(1):21-25

十屋断陷是断坳叠置的复合盆地,充填了巨厚的断陷地层。其深部地层包括登娄库组、营城组、沙河子组以及火石岭组。在十屋断陷深部沉积了沙河子组-营城组和登娄库组两套烃源岩和良好的储盖层组合;由于断陷构造运动,形成大量的断裂构造和不整合面,构成了油气运移的有效通道,同时还形成有效断裂、断鼻构造圈闭以及不整合圈闭。综合分析认为,十屋断陷深部具有有利油气成藏生储盖组合。主要有下生上储式、自生自储式和上生下储式三种成藏模式。  相似文献   

Black carbon and polycyclic aromatic hydrocarbons (PAHs) in surface sediments of China’s marginal seas   总被引：8，自引：0，他引：8  

康延菊  王旭晨  戴民汉  冯桓  李安春  宋茜 《中国海洋湖沼学报》2009,27(2):297-308

This study investigates the distribution of black carbon (BC) and its correlation with total polycyclic aromatic hydrocarbons (ΣPAH) in the surface sediments of China’s marginal seas. BC content ranges from <0.10 to 2.45 mg/g dw (grams dry weight) in the sediments studied, and varied among the different coastal regions. The Bohai Bay sediments had the highest BC contents (average 2.18 mg/g dw), which comprises a significant fraction (27%–41%) of the total organic carbon (TOC) preserved in the sediments. In ...  相似文献   

Synthesis,algal inhibition activities and QSAR studies of novel gramine compounds containing ester functional groups     

李霞  于良民  姜晓辉  夏树伟  赵海洲 《中国海洋湖沼学报》2009,27(2):309-316

  相似文献   

Accumulation of petroleum hydrocarbons and heavy metals in clams （Ruditapes philippinarum） in Jiaozhou Bay, China   总被引：2，自引：0，他引：2  

马海青  宋茜  王旭晨 《中国海洋湖沼学报》2009,27(4):887-897

Accumulation and distributions of aliphatic and polyaromatic hydrocarbons (PAHs) and heavy metals were measured in tissues of the clam Ruditapes philippinarum collected from 5 sites in Jiaozhou Bay, Qingdao, China. The concentrations of total aliphatic hydrocarbon and PAHs ranged from 570 to 2 574 ng/gdw (gram dry weight) and from 276 to 939 ng/gdw, in the most and least polluted sites, respectively. The bio-accumulation of hydrocarbons and PAHs in the clams appeared to be selective. Aliphatic hydrocarbons were predominantly represented by short chain (<nC₂₃) n-alkanes, suggesting that petroleum hydrocarbons were likely the major contamination source. The selective uptake of 3 and 4 ring PAHs, such as naphthalene, fluorene, phenanthrene, fluoranthene and pyrene, by the clams was probably related to the physiological and bio-kinetic processes that were energetically favorable for uptake of compounds with fewer rings. Accumulation of the metals Cd, Cu, Zn, Pb, Cr, Hg, and As in the clam tissues also showed high variability, ranging from 0.043 to 87 μg/gdw. Among the 7 detected metals, Zn, Cd, Cu, and As had a particularly high potential of accumulation in R. philippinarum. In general, a positive correlation was found between the tissue concentrations and sediment concentrations of hydrocarbons and of some metals. Our study suggests that moderate contamination with polyaromatic hydrocarbons, and low to moderate contamination with metals, currently exists for clam R. philippinarum in Jiaozhou Bay, in comparison with other regional studies. A long-term monitoring program is certainly needed for assessment of the potential ecological influence and toxicity of these contaminants of R. philippinarum in Jiaozhou Bay.  相似文献