中国明对虾(Fenneropenaeus chinensis)血细胞中一氧化氮合成酶的鉴定及其在白斑综合症病毒感染过程中的变化   总被引：11，自引：0，他引：11  

姜国建  于仁诚  王云峰  颜天  周名江 《海洋与湖沼》2004,35(4):342-350

诱导型一氧化氮合成酶(iNOS)在生物机体免疫，特别在无脊椎动物免疫中的作用近来得到了广泛的关注，由其催化产生的一氧化氯(NO)除具有已知的神经传导、松弛平滑肌等功能外，还具有抗菌、抗病毒、抗寄生虫等作用。作者通过硝基四氯唑蓝(NBT)法和血细胞形态观察等方法，对中国明对虾(Fenneropenaeus chinensis)血细胞中存在的诱导型一氧化氮合成酶进行了初步鉴定。在此基础上，通过亚硝酸盐法和L-瓜氨酸法对比，研究了感染白斑综合症病毒(WSSV)后中国明对虾血细胞中一氧化氯合成酶的变化情况。结果显示，中国明对虾在感染WSSV后，iNOS活性在12h内有上升趋势，实验36h后酶活性显著下降，至60h后酶活性降至对照组的一半左右。同时，被脂多糖(LPS)诱导的一氧化氮合成酶活性与对照相比也有显著下降。与此对应的是，核酸探针斑点杂交法检测病毒的结果显示：实验36h后在对虾体内能够检测到白斑综合症病毒。对照组中国明对虾血细胞的iNOS在实验过程中基本保持稳定。这说明WSSV在感染中国明对虾初期可以诱导血细胞产生iNOS，但随着WSSV在中国明对虾体内的大量增殖及其对血细胞的破坏，使得iNOS活性显著降低，对虾也趋于死亡。因此，iNOS能够作为反映对虾在病毒感染过程中健康状况的有效指标。  相似文献   

珠江口东南近海海区污损生物研究   总被引：4，自引：2，他引：4  

严涛  严文侠  董钰  梁冠和  严岩  王华接 《海洋学报》2003,25(6):117-125

为研究珠江口东南近海海区污损生物状况,于1986年5月至1987年6月,在香港东南约114和160km,水深为95和113m处布设了两个生物浮标站,并对距香港约172km,水深为115m处的Marex水文气象浮标上的污损生物群落进行调查.此次调查共采集和鉴定出污损生物78种,其中在浸海只有3个月的试板上,主要有柄蔓足类、水螅和藻类,优势种是细板条茗荷、茗荷和直杯螅.至于浸海时间达6～12个月的浮标(包括沉标)及锚链系统,其上还有相当数量的多种双壳类软体动物、无柄蔓足类和苔藓动物等硬性污损生物附着.在种类垂直分布方面,污损生物群落组成随深度发生明显变化.  相似文献   

麻痹性贝毒毒素研究Ⅰ.塔玛亚历山大藻的麻痹性贝毒毒素对运动神经末梢和全细胞钠离子通道的抑制作用研究     

王云峰  于仁诚  李钧  颜天  周名江 《海洋科学集刊》2003,(45):132-137

麻痹性贝毒(Paralytic Shellfish Poisoning，PSP)毒素由石房蛤毒素(saxitoxin，STX)及其衍生物组成，目前己发现20余种，在赤潮研究、分子生物学和神经生物学基础研究、医药、军事防化等方面都有应用潜力[其结构、类型和应用见本集刊王云峰等(2003)“麻痹性贝毒毒素的应用研究进展”一文]。由于PSP毒素的稀有来源和国际社会对STX交易的禁止，限制了国内PSP毒素应用及研究的全面深入展开，因此本文作者对PSP毒素进行了制备，并采用不同方法对制备的PSP毒素进行了研究。 PSP毒素能够选择性地可逆抑制可兴奋膜的电压依赖钠离子通道的开放，从而阻止神经冲动的发生和传导，使神经、肌肉丧失兴奋性(Frace et al.，1986；Penzotti et al.，1998)。本文利用神经束膜下记录和全细胞膜片钳技术，报道了从塔玛亚历山大藻(Alexandrium tamarense)中提取的PSP粗毒素对小鼠运动神经末梢膜电流和NG108-15细胞钠离子通道的作用研究结果，并与STX标准毒素的作用结果进行了比较。 NG108-15细胞是由小鼠神经母细胞瘤和大鼠胶质细胞瘤融合的杂交细胞，经分化剂分化后，显示诸+L1196如兴奋性、合成和释放乙酰胆碱、与培养肌细胞形成突触联系等多种神经细胞的基本特性(Hamprcht，1977)和Na+、K+、Ca2+等多种离子通道，已作为神经细胞模型被广泛应用于分析药物对离子通道作用的研究(Enomoto et al.，1992； Docherty et al.，1992；Shi et al.，1993；Hu et al.，1997a；Hu et al.，1997b)；发育出具有Na+内流支持的锋电位(Hamprecht,1977)，该电位是分析作用于膜钠离子通道药物的好材料。  相似文献   

An Experimental Study of Several Special Issues Regarding the Ice Force on Conical Structures     

SHI  Qing-zeng 《中国海洋工程》2002,16(4):561-568

The effect of the cone spacing of a conieal structure on the ice force is studied by model experiments. The ice force reduction coefficient presented in this paper expresses the relationship between the ice force and the arrangement of cones. The experiments prove that the mode of the ice failure before the boundary of upward-downward bending cone (UDBC) is crushing. A conclusion can also be drawn from the experiments that the ice force on the boundary of UDBC is by far less than that on a vertical pile with the same diameter. Moreover, the ice force frequencies on upright and inve-rted cones are obtained under the field condition of the platform JZ20-2, respectively. The results show that the alterna-tion of the ice force on UDBC can hardly induce resonance of platform JZ20-2.  相似文献   

Variation in Water Masses on Shelf of Northern Bering Sea in September 2016-2018     

Yingjie Wang  Na Liu  Lina Lin  Yan He  Bin Kong  Zhanhai Zhang 《Marine Geodesy》2020,43(3):285-301

Abstract

Based on hydrological data obtained during the 7th to 9th Chinese National Arctic Research Expeditions in the summers of 2016–2018, the main water structure on the shelf of the northern Bering Sea and the volume and heat fluxes of the Bering Strait throughflow were analyzed. Distinct variability was identified in the three Pacific water masses feeding the strait - Anadyr Water (AW), Bering Shelf Water (BSW) and Alaskan coastal water (ACW). Overall, the temperature and salinity of the entire section increased each year, with 2018 showing significant anomalies, i.e., a temperature anomaly of up to 1?°C and a maximum salinity anomaly of 2. From 2016 to 2018, the extent of the ACW gradually narrowed in the eastern part of this section, while the AW expanded eastward each year. The net volume transport through each of the three sections increased poleward from 1.65?Sv to 2.76?Sv, with the AW increasing from 0?Sv to 1.03?Sv, the BSW varying between 0.52–1.65?Sv, and the ACW gradually decreasing from 1.04?Sv to disappearing completely. The net heat fluxes were also poleward, varying between 25.77 TW and 61.50 TW, and showing a significant increase. Significant variations in magnitude and extent were observed in each water mass of the Bering Strait throughflow, which could produce widespread effects in the Arctic Ocean and the global ocean beyond.  相似文献   

Ice-Induced Fatigue Analysis by Spectral Approach for Offshore Jacket Platforms with Ice-Breaking Cones     

岳前进刘圆  张日向屈衍 王瑞学 《中国海洋工程》2007,21(1):1-10

The spectral methods and ice-induced fatigue analysis are discussed based on Miner's linear cumulative fatigue hypothesis and S-N curve data.According to the long-term data of full-scale tests on the platforms in the Bohai Sea,the ice force spectrum of conical structures and the fatigue environmental model are established.Moreover,the finite element model of JZ20-2MSW platform,an example of ice-induced fatigue analysis,is built with ANSYS software.The mode analysis and dynamic analysis in frequency domain under all kinds of ice fatigue work conditions are carried on,and the fatigue life of the structure is estimated in detail.The methods in this paper can be helpful in ice-induced fatigue analysis of ice-resistant platforms.  相似文献   

Model Test Study of Dynamic Ice Force on Compliant Conical Structures     

黄焱  史庆增宋安 《中国海洋工程》2007,21(1):11-22

To study ice-induced vibration of a compliant conical structure,a series of model tests were performed from 2004 to 2005.In the tests,the ice sheet before the compliant conical structure was found to fail in two-time breaking.From 2005 to 2006,this type of ice failure was studied through more groups of tests.The tests show that two-time breaking is the typical failure of ice before steep conical structures,and is controlled by other factors at the same time,such as ice speed and the angle of the cone.  相似文献   

Mineral provinces and matter provenance of the surficial sediments in the western Philippine Sea: implication for modern sedimentation in West Pacific marginal basin     

REN Xiangwen  YAN Quanshu  SHI Xuef  WANG Kunshan  JIANG Xiaoli 《海洋学报(英文版)》2007,26(3):44-55

The characteristics and distribution patterns of detrital minerals (0.063~0.125 mm) in marine sediments provide a significant indicator for the identification of the origin of sediment.The detrital mineral composition of 219 surface sediment samples was analysed to identify the distribution of sediments within the western Philippine Sea. The area can be divided into three mineral provinces: (I) province east of the Philippine Trench, the detrital minerals in this province are mainly composed of calcareous or siliceous organisms, with the addition of volcanogenic minerals from an adjacent island arc; (II) middle mineral province, clastic minerals including feldspar, quartz and colorless volcanic glass, sourced from seamounts with intermediate-acid volcanic rock, or erupting intermediate-acid volcano; (III) province west of the Palau—Kyūshū Ridge, the matter provenance within this province is complex; the small quantity of feldspar and quartz may be sourced from seamounts or erupting volcano with intermediate-acid composition, with a component of volcanic scoria sourced from a volcano erupting on the Palau—Kyūshū Ridge. it is suggested that, (1) Biogenic debris of the study area is closely related to water depth, with the amount of biogenic debris controlled by carbonate lysocline. (2) Volcaniclastic matter derived from the adjacent island arc can be entrained by oceanic currents and transported towards the abyssal basin over a short distance. The weathering products of volcanic rocks of the submarine plateau (e.g.,Benham Plateau) and adjacent ridges provide an important source of detrital sedimentation, and the influence scope of them is constrained by the intensity of submarine weathering. (3) Terrigenous sediments from the continent of Asia and the adjacent Philippine island arc have little influence on the sedimentation of this study area, and the felsic mineral component is probably sourced from volcanic seamounts of intermediate-acid composition.  相似文献   

深海观测平台技术     

王项南  马丽珊  熊焰  高艳波  张静 《海洋技术学报》2007,26(3):16-18

介绍了深海观测平台的重要性,以及国内外在此项技术上的发展现状,并提出了一套深海观测平台技术的设计构想以及对于未来发展方向的建议和展望。  相似文献   

锯缘青蟹NAGase的催化机理的判断     

金朱兴  张继平  颜雅雯  王勤  陈清西 《台湾海峡》2007,26(1):71-75

以p-硝基苯酚-β-D-氨基葡萄糖苷（pNP-NAG）为底物，研究产物类似物：p-羧基苯酚和苯酚对青蟹N-乙酰-β-D-氨基葡萄糖苷酶（NAGase，Ec3．2．1．52）活力的影响．结果表明：p-羧基苯酚和苯酚对该酶有抑制的作用，IC50分别为20．0和100．0mmol／dm^3．p-羧基苯酚和苯酚对酶的抑制均表现为竞争性类型，其抑制常数K1分别为5．03和25．67mmol／dm^3．结合产物N-乙酰-β-D-氨基葡萄糖（NAG）抑制作用表现为反竞争性类型，判断NAGase水解pNP-NAG反应为有序双双反应（Ordered Bi Bi）．  相似文献