闽江河口区沉积物中的抗生素分布特征及生态风险评价          下载免费PDF全文

刘四光  张乐蒙  李赫男  陈猛  陈岚  王键  张钒 《应用海洋学学报》2020,39(2):162-171

采用固相萃取和液相色谱串联质谱(HPLC-MS/MS)分析方法对闽江河口区域沉积物中8类54种抗生素的含量以及分布特征进行分析探讨,并利用熵值法对抗生素的生态风险进行评价。结果表明,闽江河口区域24个站点共检出6类(大环内酯类、磺胺类、喹诺酮类、氯霉素类、硝基咪唑类和苯并咪唑类)19种抗生素,总量范围在4.16~64.74 ng/g,平均值为17.35 ng/g;其中大环内酯类、喹诺酮类和硝基咪唑类抗生素检出频率达到100%。抗生素总量的空间分布情况呈闽江河口上游>北港>南港>河口下游的趋势,人口密集区生活污水和医疗废水排放是上游和北港沉积物大环内脂类和喹诺酮类抗生素含量较高的主要原因,而海水养殖过程中的直接投放可能是河口下游硝基咪唑类抗生素的主要来源;生态风险评价结果显示,大部分抗生素的生态风险值RQ<1,然而替米考星和甲硝唑在研究区域的RQ>1,具有一定的潜在风险,可能会对该区域生态环境产生负面效应。  相似文献   

基于SPE与SPATT的水体中麻痹性贝类毒素检测方法构建与应用   总被引：1，自引：0，他引：1  

张亚亚  闫国旺  吴海燕  谭志军  张志华  江涛  李玉 《海洋与湖沼》2020,51(2):298-306

为实现麻痹性贝类毒素(paralytic shellfish poisoning,PSP)的实时监控与提前预警,本研究构建了基于固相萃取技术(solid phase extraction,SPE)与固相吸附毒素跟踪技术(solid phase adsorption toxin tracking,SPATT)的水体中PSP检测方法,重点优化了吸附材料及前处理方法,评价了回收率、检出限等指标,并将方法应用于2019年春季秦皇岛山海关海域PSP消长过程的监测中,比较评估了两种方法的监控预警效果。结果表明:SPE方法选用ENVI-Carb 500mg/6mL固相萃取柱,过样体积为50mL,13种PSP组分的平均回收率为82.2%±10.0%、检出限为4.0-20.0ng/L;SPATT方法选用SP207大孔吸附树脂,洗脱时间为静置Id最佳,整体回收率约为9.2%;在实际应用中,结合产毒藻密度及贻贝富集毒素含量的变化,发现SPE方法的检测结果可实时表征海域PSP风险状况,对于贻贝中PSP的预警效果也显著优于SAPTT方法,后者不仅因监控方式相对滞后一个监测周期,且灵敏度及准确性均较差。对于秦皇岛海域,当SPE方法检测结果达到100ng STX eq/L时,该海域贻贝中PSP残留将具有潜在的食用安全风险,跟踪过程表明这一阈值可提前两周预警贻贝富集毒素含量超出我国限量标准(800μg STX eq/kg),这对于强化风险警示并制定防范措施具有积极作用。  相似文献   

基于车载激光点云的街景立面自动提取     

耿雨馨  钟若飞  彭宝江 《地球信息科学学报》2018,20(4):480-488

街道景观图是城市规划设计和城市管理的重要参考依据,车载点云数据能够提供沿街建筑的三维点信息,精度高,覆盖范围广泛,为街景立面整治提供了新的解决方案。为此,本文提出一种适用于车载点云的街景立面的自动提取方法,提取立面点云的具体步骤为：对原始数据去噪滤波;选取非地面点构建规则格网并二值化,依据语义特征筛选出建筑物点云;用POS数据拟合直线段帮助选取参考向量与参考平面;计算点云到参考面的距离,按距离分类点云数据,并对前述步骤中未分类点另行提取,合并面点集得到以沿街建筑物立面为主的街景立面点云。为了验证这一方法的可行性和有效性,采用点云数据进行实验,实验结果表明本方法在一定程度上提高了数据处理效率,能得到较理想的结果。  相似文献   

基于圆柱多段拟合的隧道中轴线提取方法研究     

荆海峰  李广云  王力  杨文锋 《大地测量与地球动力学》2018,38(2):211-215

提出基于圆柱多段拟合的隧道中轴线提取方法。首先对隧道点云数据进行预处理,并将点云按隧道走向分成不同区段;然后对各区段依据轴线与表面点云法线垂直关系,提取精度较低的中轴线;最后对各区段利用圆柱多段拟合,提取精度较高的中轴线。实验表明,隧道中轴线的提取在一定的采样区间具有较高的稳定性,对直线和弯曲的圆形隧道有良好的适用性,算法可靠,精度较高。  相似文献   

基于物理特征的平面流场拓扑简化算法     

秦勃  李兵  王庆江 《中国海洋大学学报(自然科学版)》2010,40(2)

基于网格合并思想实现的平面流场拓扑简化,存在物理特征被忽略和并行实现困难的问题,难以满足远程可视化的要求。文中提出1种基于物理特征的平面流场拓扑简化算法。算法通过计算生成平面流场的物理特征参数图像,利用图像分割提取平面流场的特征区域达到流场简化的目的。实验结果表明,算法能从物理特征和宏观的角度出发快速、准确描述平面流场的拓扑结构,为平面流场可视化提供了1种新方法。  相似文献   

GPS/PWV时间序列特征提取方法的研究     

胡广保  叶世榕  张彦祥  夏朋飞  夏凤雨 《大地测量与地球动力学》2019,39(1):36-40

针对GAMIT/GLOBK软件解算得到的4 a GPS/PWV时间序列的特征提取问题,提出基于滤波辅助的部分集成经验模态分解（PEEMD）与Hilbert谱分析相结合的特征提取方法。首先,在PEEMD方法的基础上,结合滤波辅助的PEEMD方法与Hilbert谱分析,建立GPS/PWV时间序列特征提取模型;然后,将所提出的方法应用于TNML测站4 a的GPS/PWV长时间序列和7 d的GPS/PWV短时间序列分析中,并将滤波辅助的PEEMD结果与传统的小波分解结果进行对比。结果表明,该特征提取方法能准确有效地提取出 GPS/PWV时间序列中的周年周期和日周期特征分量,滤波辅助的PEEMD分解结果与小波分解结果一致,且提取的特征分量与原始信号更加吻合。  相似文献   

Screening of extraction methods for glycoproteins from jellyfish (Rhopilema esculentum) oral-arms by high performance liquid chromatography     

Guoyan Ren  Bafang Li  Xue Zhao  Yongliang Zhuang  Mingyan Yan  Hu Hou  Xiukun Zhang  Li Chen 《中国海洋大学学报(英文版)》2009,8(1):83-88

In order to select an optimum extraction method for the target glycoprotein (TGP) from jellyfish (Rhopilema esculentum) oral-arms, a high performance liquid chromatography (HPLC)-assay for the determination of the TGP was developed. Purified target glycoprotein was taken as a standard glycoprotein. The results showed that the calibration curves for peak area plotted against concentration for TGP were linear (r = 0.9984, y = 4.5895x+47.601) over concentrations ranging from 50 to 400 mgL-1. The mean extraction recovery was 97.84% (CV2.60%). The fractions containing TGP were isolated from jellyfish (R. esculentum) oral-arms by four extraction methods: 1) water extraction (WE), 2) phosphate buffer solution (PBS) extraction (PE), 3) ultrasound-assisted water extraction (UA-WE), 4) ultrasound-assisted PBS extraction (UA-PE). The lyophilized extract was dissolved in Milli-Q water and analyzed directly on a short TSK-GEL G4000PWXL (7.8 mm×300 mm) column. Our results indicated that the UA-PE method was the optimum extraction method selected by HPLC.  相似文献   

Relict sand dredging for beach nourishment in the central Tyrrhenian Sea (Italy): effects on benthic assemblages     

Barbara La Porta  Monica Targusi  Loretta Lattanzi  Paola La Valle  Daniela Paganelli  & Luisa Nicoletti 《Marine Ecology》2009,30(S1):97-104

The aim of this study is to analyse the effects in space and time of relict sand-dredging activities on macrobenthic assemblages, in an area situated offshore Montalto di Castro (central Tyrrhenian Sea, Italy), and to analyse the recolonisation processes of macrobenthos in the dredged areas. The area in question is characterised by relict sand deposits (Holocenic paleo-beaches), used for beach nourishment along the Latium coast. The effects of sand extraction on benthic assemblages were investigated before, during and after three dredging operations. The sites analysed are located within the dredged areas (inside stations) and in neighbouring, not dredged, areas (outside stations). The results showed that the impact of sand extraction was confined to the dredged stations and to the areas in proximity to the dredged areas. During dredging activities, the structure of benthic assemblages within the impacted stations was characterised by low species richness and diversity. Both the direct removal of sediment and the re-suspension and consequent deposition of fine sediment affected benthic assemblages of the impacted stations. A few months after the dredgings, a recolonisation process was still observed at all the impacted stations. A gradual recolonisation process was observed at those stations affected by only one dredging, whereas a different recolonisation was observed at those stations affected by two dredgings over time. This study suggests that differences of re-colonisation processes of benthic assemblages are related to the intensity of dredging operations in terms of dredging frequency.  相似文献   

A new method for the quantification of different redox-species of molybdenum (V and VI) in seawater   总被引：1，自引：0，他引：1  

Deli Wang  Robert C. Aller  Sergio A. Saudo-Wilhelmy 《Marine Chemistry》2009,113(3-4):250-256

A new method for the direct determination of reduced and oxidized Mo species (Mo (V) and Mo (VI)) in seawater was developed and used for the first time. The method includes the complexation of Mo (V) with tartrate, solid phase extraction of the Mo (V)–tartrate complex by a XAD 7HP resin, followed by elution with acidic acetone. In this study, the eluted Mo (V) was quantified by graphite furnace atomic absorption spectrometry. The detection limit of this protocol was on the order of 0.2 nM. The analytical precision was 10% of ~ 10 nM. This method was successfully applied to the determination of Mo (V) and Mo (VI) in surface and bottom waters at the head of Peconic River Estuary. Total Mo (Mo (V) + Mo (VI)) ranged from 100–120 nM in most bottom saline waters, and 2.5–15 nM for surface fresher waters. Concentrations of Mo (V) in these environments ranged from 0 nM to ~ 15 nM, accounting for 0%–15% of the total dissolved Mo pool. The time series experiments showed that the Mo speciation changed within 1 h after the water collection, and therefore it is strongly suggested that speciation analysis be carried out within the first 15 min. However, since these are the first Mo speciation data in concentration ranges typical of normal marine and coastal waters, additional research may be required to optimize the methodology and further explore Mo cycling mechanisms.  相似文献   

基于Gabor变换和支持向量机的手写体字符识别算法     

任德昊  陈超  李文藻 《成都信息工程学院学报》2009,24(4):370-373

针对在手写字符识别中由于书写习惯和风格的不同造成字符模式不稳定的问题,将支持向量机SVM方法用于手写字符的识别.算法首先采用Gabor变换提取手写字符图像的特征参数,然后采用提取的特征训练SVM分类器.再应用SVM分类器分类和判别手写字符.实验表明这种方法具有良好的车牌识别效果,较强的鲁棒性,较大的应用价值.  相似文献