脊尾白虾(Exopalaemon carinicauda)维甲酸X受体基因克隆及其在温盐胁迫和蜕皮周期中的表达分析     

柳飞  李健  李吉涛  葛倩倩  连春盎  常志强 《海洋与湖沼》2016,47(4):828-837

为了明确脊尾白虾维甲酸X受体基因在环境胁迫和蜕皮周期中的作用,本研究通过RACE技术克隆了脊尾白虾(Exopalaemon carinicauda)维甲酸X受体c DNA全长,命名为Ec RXR基因,该c DNA序列全长为1323 bp,开放阅读框(ORF)为855 bp,编码一个284个氨基酸组成的蛋白质,分子量为30.918 k Da,理论等电点为PI为6.788;Ec RXR基因推导氨基酸序列经Blastp在线比对分析显示,Ec RXR与已知甲壳动物RXR的同源性为71%—90%;系统进化树分析结果显示,脊尾白虾Ec RXR的氨基酸序列与日本沼虾RXR的氨基酸序列聚为一支。荧光定量RT-PCR分析表明,Ec RXR基因在各组织中均有表达,而在眼柄相对表达量较高,血淋巴中最低。Ec RXR基因在蜕皮周期中的表达规律表明,Ec RXR基因表达在不同蜕皮时期存在明显变化,在眼柄、肝胰腺、胃和肠中整体呈现上升趋势,在鳃中呈现先下降后上升的趋势,在表皮中呈现逐渐降低的趋势。Ec RXR基因在温度、盐度胁迫过程中的表达规律分析结果发现,温度、盐度胁迫均可显著改变Ec RXR基因在鳃和肝胰腺中的表达模式,温度胁迫下Ec RXR基因在鳃中呈先上升后下降的表达趋势,肝胰腺中整体呈先上升后下降再上升再下降的表达趋势;盐度对鳃和肝胰腺的调控模式相同,均呈先升高后下降的变化趋势。本研究结果表明Ec RXR基因在脊尾白虾蜕皮发育、酶活性调控及渗透压调节中发挥重要作用,为深入研究甲壳动物维甲酸X受体基因的功能提供了重要的基础信息。  相似文献   

日本囊对虾Marsupenaeus japonicus)组织蛋白酶D基因克隆及表达分析     

张曼  程光平  苏永全  徐伊桦  冯文荣  王军  宋晓红  毛勇 《海洋学报》2016,38(8):52-61

组织蛋白酶D是溶酶体天冬氨酸蛋白酶家族的主要成员,广泛参与动物机体细胞内蛋白质的降解过程,对维持细胞稳态和正常代谢具有重要的作用。为研究组织蛋白酶D在甲壳动物非特异性免疫和幼体发育过程中的作用,本研究采用RACE技术首次克隆得到日本囊对虾组织蛋白酶D基因cDNA序列,命名为MjCatD,其中开放阅读框长为1161 bp,编码386个氨基酸残基。序列分析和同源建模显示该基因编码的蛋白含有保守的N-糖基化位点、天冬氨酸蛋白酶签名序列、酶活化位点和非消化性组织蛋白酶D的特征序列,并且呈保守的双叶形结构。同源性比较和系统进化分析发现,MjCatD与斑节对虾、美洲螯龙虾和脊尾白虾相似性较高,并且与它们紧密聚为一支。实时荧光定量PCR结果显示,MjCatD基因在日本囊对虾多个组织中均有表达,其中肝胰腺中表达量最高。在白斑综合征病毒(white spot syndrome virus, WSSV)感染后3～24 h日本囊对虾肝胰腺中MjCatD的表达量逐渐下降,而在48 h急剧上调至最高表达量并且与对照组差异极显著(P<0.01)。此外,MjCatD基因在幼体发育不同阶段中也表现出明显的变化趋势。以上研究表明,MjCatD基因可能参与日本囊对虾先天免疫反应和幼体发育过程。  相似文献   

Seismic attenuation estimation using a complete ensemble empirical mode decomposition-based method     

《Marine and Petroleum Geology》2016

The frequency attenuation gradient method can provide important information for hydrocarbon detection. In this paper, a method using Complete Ensemble Empirical Mode Decomposition (CEEMD), Hilbert transform and the least-squares curve-fitting is proposed for seismic attenuation estimation as an effective frequency attenuation gradient estimation approach. We first use CEEMD to obtain the different Intrinsic Mode Functions (IMFs), which have a narrow band and can enhance the physical meaning of instantaneous attributes trace by trace. The time-frequency spectrum, which is computed using a Hilbert transform of each IMF, is represented as a spectrum with a single-peak that has narrow side lobes, which is conducive to frequency attenuation gradient estimation. Second, for each time sample, the frequency-amplitude spectrum of each IMF trace is extracted from the time-frequency spectrum to conduct the attenuation gradient computation. Then, the logarithm operation is performed for each IMF trace. Due to the very narrow bands of some IMFs in some seismic traces, a variable frequency window is adopted along the IMF trace according to the local data characteristics. Finally, the attenuation gradient for each IMF in a seismic trace can be computed using least-squares fitting. A different IMF reflects a seismic trace with a different spatiotemporal scale and can highlight different geologic and stratigraphic information. The correlation weighted average operation is used to highlight some useful details in seismic trace and obtains the attenuation gradient for each seismic trace. Field data examples demonstrate our method and its effectiveness. The proposed method can stably estimate the frequency attenuation gradient.  相似文献   

Nonparametric Estimation of Undrained Shear Strength for Normally Consolidated Clays     

Iok-Tong Ng  Ka-Veng Yuen  Le Dong 《Marine Georesources & Geotechnology》2016,34(2):127-137

For decades, various well-known empirical correlations have been established and commonly used to estimate the undrained shear strength of normally consolidated clays in geotechnical practice. However, there are still many contradictory cases regarding the validity of these correlations, so their applicability and reliability are questionable. In this study, an extensive geotechnical database for normally consolidated clays has been compiled. Next, a Bayesian nonparametric method is exploited to identify the most critical soil index properties in inferring the undrained shear strength and a new predictive model is proposed. Finally, the performance of the proposed predictive model is assessed and compared with two well-known correlations using an independent testing database. It confirms that the proposed model not only possesses high predictability, but also provides superior performance over other existing empirical correlations.  相似文献   

大黄鱼白细胞介素17受体E-like的分子特征及进化分析          下载免费PDF全文

丁扬  敖敬群  艾春香  陈新华 《应用海洋学学报》2016,35(1):107-115

白细胞介素17受体(Interleukin 17 receptor,IL-17R)家族与IL-17细胞因子家族介导的各类反应有着密切联系.IL-17RE-like(IL-17REL)是一类最新发现的IL-17受体,其结构与IL-17RE相似,但尚未发现其配体.本研究克隆得到大黄鱼(Larimichthys crocea)IL-17REL(Lyc IL-17REL)的全长c DNA,其开放阅读框全长1 659个核苷酸,推断可编码一个由552个氨基酸组成的蛋白,含有信号肽、胞外段、跨膜区和胞内段.与其他物种的IL-17REL相似,Lyc IL-17REL蛋白的胞外段具有一个保守的FN I-like结构域,而胞内段缺少IL-17受体家族保守的SEFIR(SEF and IL-17R)结构域.多序列比对分析表明Lyc IL-17REL与日本河豚(Takifugu rubripes)IL-17REL的氨基酸序列同源性最高,序列一致性可达57.0%.系统进化分析表明,脊椎动物的IL-17受体家族可分为2类,IL-17RA/RB/RD为一类,IL-17RC/RE/REL为一类.其中鱼类及高等脊椎动物的IL-17REL与IL-17RE亲缘关系最近,并且推测二者可能是由同一基因从硬骨鱼类之前的物种中分化而来.组织分布表明Lyc IL-17REL在所有被检测的组织中为组成型表达,在黏膜相关组织如小肠和鳃中的表达量最高.大黄鱼受嗜水气单胞菌(Aeromonas hydrophila)诱导刺激后,Lyc IL-17REL在其头肾及鳃中的基因转录水平均显著上调.这表明Lyc IL-17REL可能在细菌感染引起的免疫反应中起着重要作用.  相似文献   

The effects of visual realism,spatial abilities,and competition on performance in map-based route learning in men     

Arzu Çöltekin  Rebecca Francelet  Kai-Florian Richter  John Thoresen  Sara Irina Fabrikant 《制图学和地理信息科学》2018,45(4):339-353

We report on how visual realism might influence map-based route learning performance in a controlled laboratory experiment with 104 male participants in a competitive context. Using animations of a dot moving through routes of interest, we find that participants recall the routes more accurately with abstract road maps than with more realistic satellite maps. We also find that, irrespective of visual realism, participants with higher spatial abilities (high-spatial participants) are more accurate in memorizing map-based routes than participants with lower spatial abilities (low-spatial participants). On the other hand, added visual realism limits high-spatial participants in their route recall speed, while it seems not to influence the recall speed of low-spatial participants. Competition affects participants’ overall confidence positively, but does not affect their route recall performance neither in terms of accuracy nor speed. With this study, we provide further empirical evidence demonstrating that it is important to choose the appropriate map type considering task characteristics and spatial abilities. While satellite maps might be perceived as more fun to use, or visually more attractive than road maps, they also require more cognitive resources for many map-based tasks, which is true even for high-spatial users.  相似文献   

基于优化经验模态分解和聚类分析的滑坡位移智能预测研究     

张凯  张科  保瑞  刘享华  齐飞飞 《岩土力学》2021,(1):211-223

针对三峡库区"阶跃式"滑坡的变形特征,提出了一种新的滑坡位移预测方法。以白水河滑坡ZG118和XD-01监测点位移数据为例,采用基于软筛分停止准则的经验模态分解(SSSC-EMD)将累计位移-时间曲线和影响因子时间序列自适应地分解为多个固有模态函数(IMF),并采用K均值(K-Means)聚类法对其进行聚类累加,得到有物理含义的位移分量(趋势性位移、周期性位移以及随机性位移)和影响因子分量(高频影响因子和低频影响因子)。使用最小二乘法对趋势性位移进行拟合预测;采用果蝇优化-最小二乘支持向量机(FOA-LSSVM)模型对周期性位移和随机性位移进行预测。将各位移分量预测值进行叠加处理,实现滑坡累计位移的预测。研究结果表明,所提出的(SSSC-EMD)-K-Means-(FOA-LSSVM)模型能够预测"阶跃式"滑坡的位移变化规律,且预测精度高于传统的支持向量机回归(SVR)、最小二乘支持向量机(LSSVM)模型;并通过改变训练集长度,进行单因素分析,发现其与预测精度之间呈正相关关系。  相似文献   

静压桩承载力时间效应的研究进展     

王永洪  黄永峰  张明义  李长河  苏雷  仉文岗  林沛元  崔纪飞  焉振 《吉林大学学报(地球科学版)》2021,51(5):1490-1505

由于静压桩沉桩后桩周土重塑,静压桩承载力表现出随着休止期的延长而增长的特性。本文从静压桩沉桩后桩周土体内孔隙水消散固结的角度出发,对静压桩承载力时间效应的理论和试验分别进行归纳,结合孔隙水消散路径及固结模型,对桩周土体初始超静孔隙水压力大小及其分布特征进行总结,分析承载力各种测试方法的优缺点,对静压桩承载力的时效性进行深化研究,并探讨了不同地质条件、不同桩的类型对休止期内静压桩承载力的影响,进一步对基于实测数据得出的经验公式进行总结。讨论了基于不同本构关系模型的应力场及位移场解答和沉桩后孔隙水压力消散解答,在此基础上总结了桩基极限承载力理论公式;探讨了黏性土、砂土条件下,考虑超固结比、不排水抗剪强度和塑性指数比对桩基极限承载力系数A的影响,在此基础上归纳了桩基极限承载力经验公式。建议在经验公式基础上设置多重参数,以提升经验公式的精确度,并完善对不同桩、土类型的参数解答;利用BP神经网络,导入静压桩承载力相关参数,以得到针对不同地质条件、桩型、休止期的承载力最优解。  相似文献   

我国华南江南春季雷暴气候特征分析     

杨波  王园香  蔡雪薇 《热带气象学报》2019,35(4):470-479

基于我国华南江南地区274个基本地面气象观测站数据、全国闪电定位数据以及欧洲中心的全球大气再分析数据(ERA-Interim),对1981—2017年华南江南地区的春季雷暴日采用经验正交函数分解方法(EOF),并与气象要素场做回归分析。得出以下主要结论:(1)我国华南江南地区春季雷暴活动高发区主要在广西东部至广东西部;其高峰期在下午18:00和凌晨4:00左右,且大多数雷暴活动持续时间不超过3 h;山区雷暴活动主要在傍晚至夜间;平原雷暴活动主要在白天,高峰在17:00及06:00前后;(2)华南江南地区的雷暴活动存在着3~5年的短周期和16年左右的长周期变化;(3)雷暴日距平EOF分析的前3个主成分累计方差贡献达到72.3%。按其向量场的方差贡献分型,Ⅰ型表现为华南江南雷暴活跃特征呈现较统一的变化规律。深厚西南低涡槽前、上干下湿的水汽层结、上冷下暖的温度层结为华南江南地区发生大范围雷暴天气提供良好的动力、水汽和位势不稳定条件,是华南江南雷暴活跃异常的主要模态;Ⅱ型表现为从华南南部到江西与浙江南部有一条西南-东北向、下宽上窄的雷暴活跃正距平异常区,而两侧为负距平异常区。其环流特征表现为温度整层偏冷,水汽整层偏湿,而西南槽前动力抬升有利于水汽抬升凝结触发对流形成雷暴;Ⅲ型表现为华南和江南地区雷暴活跃特征呈南北反位相异常,其分界线在26 °N附近。其环流特征表现为较强的干冷空气南下与南方暖湿空气在南岭山区对峙形成异常的垂直环流圈。在其上升支,低层干冷空气被卷入中高层使得中高层暖湿空气凝结释放潜热形成对流,造成华南地区多雷暴发生,而江南地区处于垂直环流的下沉支,整层湿度偏干,造成江南地区雷暴相对偏少。   相似文献   

Degradation of rock fracture asperities in unloading,reloading, and reversal     

Pooyan Asadollahi  Fulvio Tonon 《国际地质力学数值与分析法杂志》2011,35(12):1334-1346

Among the constitutive models for rock fractures developed over the years, Barton's empirical model has been widely used. Although Barton's failure criterion predicts peak shear strength of rock fractures with acceptable precision, it has some limitations in estimating the peak shear displacement, post‐peak shear strength, dilation, and surface degradation. The first author modified Barton's original model in order to address these limitations. Barton proposed his model for degradation of fracture asperities in unloading, reloading, and shear displacement reversal based on just one cyclic direct shear test. In this study, a database of results of 18 cyclic direct shear tests available in the literature was collected and analyzed. Modifications were made to Barton's original model (in terms of fracture cyclic shearing) to make it consistent with the modified model proposed by the first author. Copyright © 2010 John Wiley & Sons, Ltd.  相似文献