蛋白质组学的研究概况及其在海洋生物学中的应用   总被引：7，自引：0，他引：7       下载免费PDF全文

高政权  王广策  曾呈奎 《海洋与湖沼》2003,34(3):334-344

随着DNA序列测定技术的飞速发展，人们可以在很短的时间内获得某种生物基因组的全序列。生物体的生命活动主要依赖于基因组的产物——蛋白质组，但是蛋白质组的研究相对于基因组来说却要复杂得多。蛋白质组技术是在细胞内整体蛋白质水平上进行研究，从蛋白质整体活动的角度来研究生命活动的规律。综述了蛋白质组和蛋白质组学出现的背景、起源、概念、研究技术、研究内容、面对的挑战、开展该项研究的意义、陆生动植物和微生物蛋白质组学的研究进展及海洋生物蛋白质组学的研究概况。目前，普通生物方面的蛋白质组工作开展得较多，海洋生物方面却相对滞后。种种迹象表明，开展海洋生物蛋白质组学研究，对揭示生命的奥秘、促进海洋资源的开发都有重大的推动作用。  相似文献   

Spatial and temporal variability of microplankton and detritus, and their export to the shelf sediments in the upwelling area off Concepción, Chile (∼36°S), during 2002-2005     

Humberto E. González  Eduardo Menschel  Carola Aparicio 《Progress in Oceanography》2007,75(3):435-451

Seasonal variations in diversity and biomass of diatoms, tintinnids, and dinoflagellates and the contribution of microplankton and faecal material to the vertical flux of particulates were investigated at one time series station T (station 18) between 2002 and 2005 and at a grid of stations during November 2004 in the coastal and oceanic area off Concepción (36°S), Chile. The variations were analysed in relation to water column temperature, dissolved oxygen, nutrient concentration, offshore Ekman transport, and chlorophyll-a concentration. Abundance was estimated as cell numbers per litre and biomass in terms of biovolume and carbon units.A sharp decrease with depth was observed in the abundance of both phytoplankton and microzooplankton during the whole annual cycle; over 70% of their abundance was concentrated in the upper 10 m of the water column. Also, a clear seasonality in microplankton distribution was observed at station T, with maxima for diatoms, tintinnids, and dinoflagellates every summer (centred on January) from 2002 to 2005.On the grid of stations, the maximum integrated (0-50 m) micro-phytoplankton abundances (>1 × 10⁹ cells m⁻²) occurred at the coastal stations, an area directly influenced by upwelling. A similar spatial distribution was observed for the integrated (0-200 m) faecal carbon (with values up to 632 mg C m⁻²). Tintinnids were distributed in all the first 300 miles from the coast and dinoflagellates were more abundant in oceanic waters.At station T, the average POC export production (below 50 m depth) was 16.6% (SD = 17%; range 2-67%; n = 16). The biological-mediated fluxes of carbon between the upper productive layer and the sediments of the continental shelf off Concepción depend upon key groups of phytoplankton (Thalassiosira spp., Chaetoceros spp.) and zooplankton (euphausiids) through the export of either cells or faecal material, respectively.  相似文献   

氯甲烷的海洋生物地球化学研究     

陆小兰  杨桂朋  高先池 《中国海洋大学学报(自然科学版)》2006,36(6):868-874

对氯甲烷的海洋生物地球化学循环的研究进展进行述评。介绍了氯甲烷在海洋环境中的来源、分布、去除、海-气通量、大气氯甲烷的源、汇估算及海水中氯甲烷的分析方法等方面,并提出在国内海域进行氯甲烷研究的几点设想。  相似文献   

西太平洋暖池区海—气通量计算分析   总被引：4，自引：0，他引：4  

徐天真  陈伯海 《中国海洋大学学报(自然科学版)》1993,(Z2)

用J.Launiaimen和T.Vihma提出的近地面层湍流通量计算方法,对我国在1992年11月至1993年2月TOGA—COARE—IOP实验中所获资料计算处理。得出所在站位的海一气间显效、潜热及动量通量。指出西大平洋暖池海区游热通量与显效通量之比为10.14:1;风速大于8m/s后各通量随风速的变化率明显增加;动量与热量的块体通量系数Cd和Ce,h随风速变化有相似的规律;Monin—Obukhov大气稳定度参数Z/L与△T/U_(10)之间有较好的统计关系。  相似文献   

弱地形上内潮生成问题——Ⅱ.粗糙地形情况下水深对内潮能通量的影响     

戴德君  乔方利  袁业立 《海洋科学进展》2007,25(3):247-256

前人在讨论水深对内潮能通量影响的时候得出结论:有限深海洋中海面对内潮的反射使得正压潮向内潮的能量转化相比较无限深海的情况显著降低,对于选定的地形,在无限深海假定下得到的能通量是该地形上内潮能通量的上限。鉴于前人所研究的基本上都是平滑的地形,而实际的海洋地形总是比较粗糙的,本文探讨了粗糙地形上内潮能通量随水深的变化。选取了弦函数地形、随机白噪声地形、弦函数地形叠加在高斯地形之上、随机白噪声地形叠加在高斯地形之上和随机白噪声地形与弦函数地形同时叠加在高斯地形之上5种情况进行了研究,发现对于这5种情况,都存在海洋有限深时的能通量大于无限深假定时的能通量,这说明前人得出的"有限深海洋中海面对内潮的反射使得正压潮向内潮的能量转化相比较无限深海的情况显著降低"的结论对于粗糙地形并不适用。  相似文献   

南黄海夏季海水pCO2研究Ⅱ--下层海水涌升和长江冲淡水对海-气界面CO2通量的贡献     

江春波  张龙军  王峰 《中国海洋大学学报(自然科学版)》2006,36(Z1):147-152

根据2001年7月对南黄海的大面积调查,研究了南黄海夏季pCO2的分布机制,着重讨论下层海水涌升和长江冲淡水对海-气界面CO2通量的贡献,并给出了南黄海海-气界面CO2通量。研究结果表明:夏季南黄海总体上是CO2的1个弱源,大约向大气中释放45.05×104t C。夏季南黄海表层海水pCO2分布表现出了极大的不均性,其汇区主要由长江冲淡水造成,影响区域占汇区吸收CO2的99.9%;而在源区,下层海水涌升虽然面积较小却占源区释放CO2的35.2%。可见陆架边缘海区源/汇格局的地域差异非常之特别。  相似文献   

长江中下游6月份降水的一个预报指标     

赵永平  陈永利 《海洋科学》1995,19(1):39-42

根据１９６３－１９９２年嵊山海洋站２月海气感热输送和２２ａ太阳磁周期与降水的关系，提出了一个长江中下游６月降水的综合预报指标，用此指标，对１９９３年６月长江中下游降水进行回报，结果与实况一致。  相似文献   

台湾海峡海流结构及海水通量     

傅子琅  胡建宇 《热带海洋学报》1995,(3)

主要根据台湾海峡的实测海流资料，以夏、冬为代表季节，分析了台湾海峡２—３个纬向断面的海流结构，计算出各断面的海水通量。结果表明：夏季，台湾海峡中、北部海域各层的海流一般偏Ｎ向流动，Ｎ向的海水净通量为３．３２×１０６ｍ３·ｓ－１；冬季，高温高盐的黑潮水和南海水由南向北经南部断面进入台湾海峡，其海水通量分别为１．６９×１０６ｍ３·ｓ－１和０．５９×１０６ｍ３·ｓ－１；而东海水由北向南通过北部断面进入台湾海峡，其海水通量为１．０２×１０６ｍ３·ｓ－１，其中，有０．４０×１０６ｍ３·ｓ－１的海水沿着福建和广东近岸流进南海，其余０．６２×１０６ｍ３·ｓ－１的海水在台湾海峡北部混合后随同黑潮水和南海水流入东海。总之，流经台湾海峡的Ｎ向海水净通量为１．７４×１０６ｍ３·ｓ－１。  相似文献   

Chemical Fluxes of Asian Rivers into Oceans and their Controlling Factors     

杨守业  李从先  刘曙光 《海洋通报(英文版)》2001,3(2)

Chemical fluxes of Asian rivers into oceans bear different regional variations. Three zones are characteristic of distinct dissolved sediment loads and yields and ionic concentration.Rivers into oceans in China play an important role in Asia because of their different chemical fluxes, among which those draining the Loess Plateau have high ionic concentration, low water discharges and dissolved sediment loads and yields.Climate, vegetation, soil and strata lithology, chemical weathering intensity and tectonic activity dominate chemical fluxes of Asian rivers into oceans, and different factors have different effects on the chemical fluxes of separate regional rivers. Rising of the Tibet Plateau also exerts an important influence on chemical compositions of rivers originating from it.  相似文献   

Assessment of the effects of hydrocarbon contamination on the sedimentary bacterial communities and determination of the polar lipid fraction purity: Relevance of intact phospholipid analysis     

Nicolas Mazzella  Josiane Molinet  Agung Dhamar Syakti  Jean-Claude Bertrand  Pierre Doumenq   《Marine Chemistry》2007,103(3-4):304-317

This in situ study reports on the relevance of intact phospholipids as new biomarkers and highlights the limits of the classical fatty acid analysis after the hydrolysis of the polar lipid fraction. The analysis of polar lipid fractions revealed significant contributions of marine macrophytes with either non-phospholipid compounds (sulfoquinovosyl diacylglycerol) or with both non-bacterial phospholipid classes (phosphatidylcholine) and non-bacterial phospholipid molecular species (some molecular species of phosphatidylglycerol). The analysis of intact phospholipids showed also the predominance of gram-negative bacteria in sediments whereas the analysis of fatty acids alone cannot confirm such information. Lastly, as regard to the effects of petroleum hydrocarbons on the sedimentary bacterial communities, we observed a higher degree of saturation of the fatty acyl chains of the phospholipids extracted from sediment exposed to a massive and chronic crude oil contamination. Such a result was interpreted as an adaptive response resulting in higher membrane rigidity of the bacterial communities.  相似文献