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31.
产复合酶菌株Pseudomonas sp.NJ197产酶发酵条件的研究   总被引:2,自引:0,他引:2  
张金伟  曾润颖 《台湾海峡》2005,24(4):520-525
从南极普利兹湾深海900m的沉积物中筛选到一株同时产多种低温复合酶的菌 株NJ197,作者对其进行碳源、氮源、无机盐、起始pH值、培养时间、接种量等发酵条 件的优化实验.实验结果证明,在以0.5%可溶性淀粉为碳源,以0.5%豆饼粉为氮 源,无机盐:0.424%NH4H2PO4、0.075%K2HPO4、0.02%MgSO4、0.5%CaCO3,起始 pH值8.5,接种36h种龄的种子10%,20℃、250r/min的条件下在旋转摇床中培养 72h,产复合酶菌株产脂肪酶和淀粉酶活性最高.最佳的生理条件下复合酶中的碱性 脂肪酶和淀粉酶的产量分别提高到22.4U/cm3和30.9U/cm3,该产复合酶菌株可以 作为诱变育种、基因工程菌改造的出发菌株,在洗涤和制革工业中有良好的应用前 景.  相似文献   
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闽江口水动力和污染扩散数值模拟   总被引:3,自引:0,他引:3  
江毓武  张珞平 《台湾海峡》1997,16(2):169-173
本文建立了闽江口二维水动力、污染扩散数值模型,模型采用ADI双向隐式求解。在水动力模拟中,对岸界的丁坝有用线性边界进行拟合,并考虑到河口区侧向摩擦作用;在污染扩散模拟中,用动态拟合的方法对浓度水边界进行了较为合理的改进。计算结果表明,本模型是成功的。  相似文献   
34.
Thirteen vertical profiles of 226Ra and 222Rn in the near-surface water were obtained in the western North Pacific in winter, and the gas transfer velocities across the air-sea interface were estimated. The transfer velocities found by applying a steady state model varied widely from 2.1 to 30.2 m day−1 with a mean of 9.4 m day−1. The mean value is almost 5 times higher than that in summer in other oceans, and the maximum value is a record high for world oceans. This is partly due to the inadequacy of the steady state model, which overestimates when stronger winds blow in more recent days than the 222Rn half-life of about 4 days. In fact, a strong low pressure zone passed through the station about 2 days earlier, which was one of the low pressure zones that with a period of develop once a week or so in the northwestern North Pacific in winter. Instead of steady-state removal, if half of the radon removal occurred sporadically every 7 days, and the last removal took place two days before the observation, the transfer velocity would be 26 m day−1. Our mean transfer velocity, which is less than 20% different from the steady state value including both overestimated and underestimated values, 9.4 ± 4.8 m day−1, seems to represent the mean state of this region in winter. This suggests that the gas exchange fluxes under extremely rough conditions in the open ocean are larger than those estimated by using a transfer velocity equation with a linear or quadratic relationship with wind speed. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   
35.
蓝东兆  程兆第 《台湾海峡》1995,14(3):235-240
本文根据分析结果阐述了两种不同样品处理方法所获得的硅藻丰度和微型硅藻的人主要种类和相对百分含量。研究表明洗涤富集法所获得的硅藻丰度存在较大误差;微型硅藻的含量普遍介于10%-20%之间,最高可达42.31%,最低为5.66%,并指出了在微型硅藻相对含量大于20%的样品中 ,其主要种类为具槽直链藻和条纹小环藻等沿岸底格格不入中,并成群体产出。  相似文献   
36.
A finite-difference scheme and a modified marker-and-cell (MAC) algorithm have been developed to investigate the interactions of fully nonlinear waves with two- or three-dimensional structures of arbitrary shape. The Navier–Stokes (NS) and continuity equations are solved in the computational domain and the boundary values are updated at each time step by the finite-difference time-marching scheme in the framework of a rectangular coordinate system. The fully nonlinear kinematic free-surface condition is implemented by the marker-density function (MDF) technique developed for two fluid layers.To demonstrate the capability and accuracy of the present method, the numerical simulation of backstep flows with free-surface, and the numerical tests of the MDF technique with limit functions are conducted. The 3D program was then applied to nonlinear wave interactions with conical gravity platforms of circular and octagonal cross-sections. The numerical prediction of maximum wave run-up on arctic structures is compared with the prediction of the Shore Protection Manual (SPM) method and those of linear and second-order diffraction analyses based on potential theory and boundary element method (BEM). Through this comparison, the effects of non-linearity and viscosity on wave loading and run-up are discussed.  相似文献   
37.
S.K. Lee  H. Choi  S. Surendran 《Ocean Engineering》2006,33(17-18):2454-2465
The effects of free-surface waves on the floating structures are of great importance in the offshore industry. Among the six degrees of motions of a surface ship the absence of restoring forces in surge, sway and yaw led to critical situations for moored ships in the recent times. The order of forces in horizontal plane and their exciting frequencies are matters of interest. The resonance with the presence of moored chains led to many accidents in the recent past. The lines in dry conditions may not give good damping and in wet condition they may trigger the system to chaotic motions and jumps. Two different loading conditions of a container ship model are tested with waves in laboratory conditions in two different drafts. The mooring lines are chosen as per scale law and the energy under the response spectrum is determined from the plots. The results give new insights into the movement of a berthed ships subjected to waves. Response of the moored ship to different loading conditions in different water depths are discussed in this paper. The paper gives the order of energy due to first-order and slowly varying movement of a berthed container model in a towing tank.  相似文献   
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本文阐述了莱州湾东部滨海砂金的物源、地貌、水动力、第四系、新构造运动和海平面变化等成矿条件以及砂金的富集特点,对该区砂金的成矿远景进行了区划和评价,初步认为该区具有良好的砂金成矿远景。  相似文献   
40.
中国龙虾生长特点及促生长途径的探讨   总被引:1,自引:0,他引:1  
蜕壳后新甲壳完全硬化之前是中国龙虾(Panulirus stimpsoni)个体蜕壳生长过程的延续,对中国龙虾正常快速生长具有重要意义。环境因子、营养条件对中国龙虾的蜕壳与生长具有显著影响。24-27℃是中国龙虾蜕壳生长的适温范围;盐度24-32适宜中国龙虾生长,最适生长盐度是28;在6种试验饵料中翡翠贻贝饲养中国龙虾效果最好,其次是杂虾、杂蟹、牡蛎,投喂杂鱼的中国龙虾蜕壳增长率和阶段生长率都很低;龙虾饲料中添加蜕壳素能够縮短蜕壳周期,实现蜕壳率和蜕壳增长率的同步增长。摘除眼柄也能促进中国龙虾的蜕壳与生长,电刺激等外源刺激则只能促进蜕壳,而不能加速中国龙虾的生长,甚至可能产生负面影响。中国龙虾生长存在种内性别间的差异,同等条件下雄性个体的蜕壳增长率高于雌性个体。  相似文献   
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