Importance of seagrass as a carbon source for heterotrophic bacteria in a subtropical estuary (Florida Bay)     

Clayton J. Williams  Rudolf Jaffé  William T. Anderson  Frank J. Jochem 《Estuarine, Coastal and Shelf Science》2009

A stable carbon isotope approach was taken to identify potential organic matter sources incorporated into biomass by the heterotrophic bacterial community of Florida Bay, a subtropical estuary with a recent history of seagrass loss and phytoplankton blooms. To gain a more complete understanding of bacterial carbon cycling in seagrass estuaries, this study focused on the importance of seagrass-derived organic matter to pelagic, seagrass epiphytic, and sediment surface bacteria. Particulate organic matter (POM), seagrass epiphytic, seagrass (Thalassia testudinum) leaf, and sediment surface samples were collected from four Florida Bay locations with historically different organic matter inputs, macrophyte densities, and primary productivities. Bulk (observed and those reported previously) and compound-specific bacterial fatty acid δ¹³C values were used to determine important carbon sources to the estuary and benthic and pelagic heterotrophic bacteria. The δ¹³C values of T. testudinum green leaves with epiphytes removed ranged from −9.9 to −6.9‰. Thalassia testudinum δ¹³C values were significant more enriched in ¹³C than POM, epiphytic, and sediment samples, which ranged from −16.4 to −13.5, −16.2 to −9.6, and −16.7 to −11.0‰, respectively. Bacterial fatty acid δ¹³C values (measured for br14:0, 15:0, i15:0, a15:0, br17:0, and 17:0) ranged from −25.5 to −8.2‰. Assuming a −3‰ carbon source fractionation from fatty acid to whole bacteria, pelagic, epiphytic, and sediment bacterial δ¹³C values were generally more depleted in ¹³C than T. testudinum δ¹³C values, more enriched in ¹³C than reported δ¹³C values for mangroves, and similar to reported δ¹³C values for algae. IsoSource mixing model results indicated that organic matter derived from T. testudinum was incorporated by both benthic and pelagic bacterial communities, where 13–67% of bacterial δ¹³C values could arise from consumption of seagrass-derived organic matter. The IsoSource model, however, failed to discriminate clearly the fraction of algal (0–86%) and mangrove (0–42%) organic matter incorporated by bacterial communities. These results indicate that pelagic, epiphytic, and sediment surface bacteria consumed organic matter from a variety of sources. Bacterial communities incorporated consistently seagrass-derived organic matter, the dominant macrophyte in Florida Bay, but seagrass δ¹³C values alone could not account fully for bacterial δ¹³C values.  相似文献   

A Study on the Contribution of Different Food Sources to Shrimp Growth in an Intensive Fenneropenaeus chinensis Pond     

SU Yuepeng  MA Shen  TIAN Xiangli  DONG Shuanglin 《中国海洋大学学报(英文版)》2008,7(4)

Stable isotope methods can be used to determine the food sources and prey items of aquatic organisms accurately andreliably.This study examined the relative contribution of artificial foods (the formulated feed and Artemia) and natural foods toshrimp growth in an intensive Fenneropenaeus chinensis pond by using carbon and nitrogen stable isotopes.The results showed that the nutrition utilization efficiency of the harvested shrimp was low,only 33.18% of feed nitrogen and 21.73% of feed carbon beingconverted to shrimp flesh.Our stable isotope results showed that the shrimp obtained nutrition for maximum growth from artificialfoods,whose contribution was 93.5%,with the remaining attributed to the natural foods.However,there was 0.94 t harvested shrimpderived from natural foods (the rest of 13.56t harvested shrimp derived from artificial foods) in lha intensive pond with a shrimpproduction of 14.50 tha-1.Therefore,unit area shrimp production can be increased by increasing the contribution proportion of natu-ral foods in intensive shrimp farming.  相似文献   

Isotopic composition (δ18O and δD) of precipitation and groundwater in a semi‐arid,mountainous area (Guadiana Menor basin,Southeast Spain)     

F. Fernndez‐Chacn  J. Benavente  J. C. Rubio‐Campos  C. Kohfahl  J. Jimnez  H. Meyer  H. Hubberten  A. Pekdeger 《水文研究》2010,24(10):1343-1356

We characterize the precipitation and groundwater in a mountainous (peaks slightly above 3000 m a.s.l.), semi‐arid river basin in SE Spain in terms of the isotopes ¹⁸O and ²H. This basin, with an extension of about 7000 km², is an ideal site for such a study because fronts from the Atlantic and the Mediterranean converge here. Much of the land is farmed and irrigated both by groundwater and runoff water collected in reservoirs. A total of approximately 100 water samples from precipitation and 300 from groundwater have been analysed. To sample precipitation we set up a network of 39 stations at different altitudes (800–1700 m a.s.l.), with which we were able to collect the rain and snowfall from 29 separate events between July 2005 and April 2007 and take monthly samples during the periods of maximum recharge of the aquifers. To characterize the groundwater we set up a control network of 43 points (23 springs and 20 wells) to sample every 3 months the main aquifers and both the thermal and non‐thermal groundwater. We also sampled two shallow‐water sites (a reservoir and a river). The isotope composition of the precipitation forms a local meteoric water line (LMWL) characterized by the equation δD = 7·72δ¹⁸O + 9·90, with mean values for δ¹⁸O and δD of − 10·28‰ and − 69·33‰, respectively, and 12·9‰ for the d‐excess value. To correlate the isotope composition of the rainfall water with groundwater we calculated the weighted local meteoric water line (WLMWL), characterized by the equation δD = 7·40δ¹⁸O + 7·24, which takes into account the quantity of water precipitated during each event. These values of (dδD/dδ¹⁸O)< 8 and d‐excess (δD–8δ¹⁸O)< 10 in each curve bear witness to the ‘amount effect’, an effect which is more manifest between May and September, when the ground temperature is higher. Other effects noted in the basin were those of altitude and the continental influence. The isotopic compositions of the groundwater are represented by the equation δD = 4·79δ¹⁸O − 18·64. The groundwater is richer in heavy isotopes than the rainfall, with mean values of − 8·48‰ for δ¹⁸O and − 59·27‰ for δD. The isotope enrichment processes detected include a higher rate of evaporation from detrital aquifers than from carbonate ones, the effects of recharging aquifers from irrigation return flow and/or from reservoirs' leakage and enrichment in δ¹⁸O from thermal water. Copyright © 2010 John Wiley & Sons, Ltd.  相似文献   

Variability in the fractionation of stable isotopes during degradation of two intertidal red algae     

Jaclyn M. Hill  Christopher D. McQuaid 《Estuarine, Coastal and Shelf Science》2009

Macroalgae contribute to intertidal food webs primarily as detritus, with unclear implications for food web studies using stable isotope analysis. We examined differences in the thallus parts of two South African rhodophytes (Gelidium pristoides and Hypnea spicifera) and changes in overall δ¹³C, δ¹⁵N signatures and C:N ratios during degradation in both the field and laboratory. We hypothesized that both degrading macroalgal tissue and macroalgal-derived suspended particulate material (SPM) would show negligible changes in δ¹³C, but enriched δ¹⁵N signatures and lower C:N ratios relative to healthy plants. Only C:N laboratory ratios conformed to predictions, with both species of macroalgae showing decomposition related changes in δ¹³C and significant depletions in δ¹⁵N in both the field and laboratory. In the laboratory, algal tissue and SPM from each species behaved similarly (though some effects were non-significant) but with differing strengths. Gelidium pristoides δ¹³C increased and C:N ratios decreased over time in tissue and SPM; δ¹⁵N became depleted only in SPM. Hypnea spicifera, δ¹³C, δ¹⁵N and C:N ratios all decreased during degradation in both SPM and algae.  相似文献   

Spatial variability of nitrogen isotope ratios of particulate material from Northwest Atlantic continental shelf waters     

R.A. McKinney  A.J. Oczkowski  J. Prezioso  K.J.W. Hyde 《Estuarine, Coastal and Shelf Science》2010

Human encroachment on the coastal zone has led to concern about the impact of anthropogenic nitrogen (N) on estuarine and continental shelf waters. Western North Atlantic watershed budgets suggest that the export of human-derived N from estuaries to shelf waters off the east coast of the US may be significant; however, models based on water inputs and estimates of upwelling of deepwater nutrients to surface waters of the mid-Atlantic bight indicate that estuarine N may be a relatively minor component of the overall shelf N budget. Stable N isotope ratios could provide a means to assess the relative input of anthropogenic N to shelf waters, particularly since dissolved N from human sources has elevated δ¹⁵N values (range: 7–30‰). We collected particulate material from surface shelf waters off the US east coast from 2000 to 2005 at near-shore sample sites proximal to the mouth of six estuaries and corresponding sites farther offshore. Near-shore (mean 33.7 km from estuary mouth) δ¹⁵N values ranged from 5.5 to 7.7‰ Offshore values (mean 92.4 km from estuary mouth) were consistently lower than near-shore sites (average 4.7 ± 1.0‰ versus 6.8 ± 1.1‰), suggesting different N sources to near and offshore stations. Near-shore regions are often more productive, as mean monthly chlorophyll-a concentrations from the sea-viewing wide field-of-view sensor (SeaWiFS) were significantly higher at near-shore sites near the mouth of three of the six estuaries. A mass balance using a concentration-dependent mixing model with chlorophyll-a concentrations as a surrogate for dissolved inorganic nitrogen can account for all of the nitrogen at near-shore sites south of Cape Cod with estuarine nitrogen estimated to contribute 45–85% of the nitrogen to the near-shore surface particulate material. Our results support the hypothesis that estuarine nitrogen is influencing continental shelf ecosystems, and also provide preliminary evidence of the spatial extent of its influence on shelf waters in the mid-Atlantic bight.  相似文献   

Modelling future conditions in the degraded semi-arid estuary of Australia's largest river using ecosystem states     

Rebecca E. Lester  Peter G. Fairweather 《Estuarine, Coastal and Shelf Science》2009

Over the last ten years, there has been a major decline in the condition of the Coorong, the estuary for Australia's largest river system, the River Murray. This decline is due to prolonged drought combined with past management of the Murray-Darling Basin. In order to successfully manage the estuary in the future, predictions are needed to evaluate the effect of possible management actions on the Coorong ecosystem under a variety of climatic scenarios. The alternative stable state concept can be extended to non-equilibrium systems, allowing for modelling of condition. Rather than constraining the definition of alternative states to those that are stable, we identify a suite of ecosystem states that occur naturally, but also include those that arise during the decline of the system. Eight distinct states were defined, with thresholds between them based on a combination of environmental characteristics associated with co-occurring biota. Threshold values for environmental characteristics define the transition rules between states. Mapping these states allows us to characterise the condition of the estuary in both space and time. The distribution of these states, and the diversity of states supported can be used to create an ecosystem condition index. By calculating the value of the index over time, the trajectory of ecosystem condition merges, and predictions can be made about future condition, should the current situation continue. This trajectory modelling can then form a baseline against which to evaluate possible management actions under a variety of climatic scenarios to identify those most likely to improve the condition of the Coorong.  相似文献   

Effects of preservatives on stable isotope analyses of four marine species     

Sirka Carabel  Patricia Verísimo  Juan Freire 《Estuarine, Coastal and Shelf Science》2009

The aim of the present study is to quantify the effect of formalin–ethanol preservation on the carbon and nitrogen stable isotope signatures of four taxonomical groups of marine species (Himanthalia elongata, Anemonia sulcata, Mytilus galloprovincialis and Patella vulgata). To examine temporal changes in the effects of preservation and to determine if preservation induced predictable shifts in δ¹³C and δ¹⁵N signatures, repeated analyses were carried out after 6, 12 and 24 months of preservation. Data from our study showed highly variable effects of the formalin–ethanol preservation on carbon and nitrogen isotope signatures between species. The use of a general correction factor was not possible, or else it should be species-specific. Differences in nitrogen isotopic values between preserved and unpreserved samples were minor compared to the assumed enrichment between trophic levels. The combined use of data from preserved and unpreserved samples could lead to biases in the estimation of the trophic level of organisms. Changes that preservatives caused in carbon values were variable between species and not always small enough to be ignored. So the use of data from preserved samples could change the interpretation of the mixing models used to determine the importance of multiple sources of carbon. In order to elucidate the effects that preservatives have in other species, further studies will be necessary.  相似文献   

Environmental change in northern Belize since the latest Pleistocene     

Sarah Metcalfe  Ann Breen  Malcolm Murray  Peter Furley  Anthony Fallick  Angus McKenzie 《第四纪科学杂志》2009,24(6):627-641

Cores from the New River Lagoon in Belize have been analysed using a range of palaeolimnological proxies to reconstruct change since the latest Pleistocene. The combination of diatom and stable isotope analyses (supported by mineralogical and major element data) indicate that the New River Lagoon has been a freshwater system throughout the period of record. For most of this time the lagoon has been hydrologically open, but there are indications that it may have become closed in the latest Pleistocene or very early Holocene. This may have been associated with a drier climate and lower sea level. Mid‐Holocene records are consistent with those from elsewhere in the region, indicating a stable and moist climate. The late Holocene was marked by greater variability, but there is no clear evidence of any drying in the late Classic coincident with the Maya ‘collapse’ ca. AD 900. Our results indicate that the continuity of occupation at the Maya site of Lamanai on the shores of the New River Lagoon is consistent with continued access to freshwater during periods of regional climatic variability. The importance of conditions at individual sites when considering both system response and human vulnerability to climate change is emphasised. Copyright © 2009 John Wiley & Sons, Ltd.  相似文献   

Climate change during the past 1000 years: a high‐temporal‐resolution multiproxy record from a mire in northern Finland     

Walter Finsinger  Kristian Schoning  Sheila Hicks  Andreas Lücke  Tomasz Goslar  Friederike Wagner‐cremer  Heidi Hyyppä 《第四纪科学杂志》2013,28(2):152-164

We present a record of peatland development in relation to climate changes and human activities from the Palomaa mire, a remote site in northern Finland. We used fine‐resolution and continuous sampling to analyse several proxies including pollen (for vegetation on and around the mire), testate amoebae (TA; for mire‐wetness changes), oxygen and carbon isotopes from Sphagnum cellulose (δ¹⁸O and δ¹³C; for humidity and temperature changes), peat‐accumulation rates and peat‐colour changes. In spite of an excellent accumulation model (30 ¹⁴C dates and estimated standard deviation of sample ages <1 year in the most recent part), the potential to determine cause–effect (or lead–lag) relationships between environmental changes and biotic responses is limited by proxy‐specific incorporation processes below the actively growing Sphagnum surface. Nevertheless, what emerges is that mire development was closely related to water‐table changes rather than to summer temperature and that water‐table decreases were associated with increasing peat‐accumulation rates and more abundant mire vegetation. A rapid fen‐to‐bog transition occurred within a few years around AD 1960 when the water table decreased beyond the historical minimum, supporting the notion that mires can rapidly shift into bogs in response to allogenic factors. Copyright © 2012 John Wiley & Sons, Ltd.  相似文献   

An insight into the dynamics of Lake Nainital (Kumaun Himalaya,India) using stable isotope data / Un aperçu de la dynamique du Lac Nainital (Himalaya Kumaun,Inde) à partir de données isotopiques stables     

《水文科学杂志》2013,58(6)

Abstract

Abstract The water balance of Lake Nainital in the Kumaun Himalaya, India was previously computed using water budgeting and other indirect methods. An important data set of stable oxygen and hydrogen isotopic composition of water sources of the lake region was also presented and used to verify the annual estimates of subsurface flow/water balance. In the present study, the same data set has been used to investigate the dynamics of this lake in terms of the seasonal processes operative during the annual hydrological cycle: increased inflow during the monsoon, delayed groundwater inflow, and stratification and mixing of water. Based on the available data, a simple two-box model was used to constrain the values of exchange coefficients between the hypolimnion and epilimnion layers and to estimate evaporation and outflow components from the isotopic data.  相似文献