用16S rRNA基因的内切酶图谱快速鉴别几种对虾病原菌   总被引：4，自引：0，他引：4  

孔杰  刘萍  张岩  杨丛海  叶军  徐怀恕  郭华荣 《海洋与湖沼》1997,28(5):449-452

坎普氏弧菌是青岛海洋大学生物系微生物实验室于１９８９－１９９０年自对虾养殖场中国对虾红腿病心脏及血淋巴中分离并鉴定的菌株，副溶血菌和溶藻胶弧菌两菌株于１９９４年９月得自中国科学院微生物研究所，为建立快速、准确的中国对虾病原菌的诊断技术，根据几种细菌的１６ＳｒＲＮＡ基因的序列，设计并合成该基因的多聚酶反应的引物ＰＬ１和ＰＬ２。并用该对引物分别从坎普氏弧菌、副溶血弧菌和溶藻胶弧菌的ＤＮＡ要品中扩增出分  相似文献   

几株原甲藻核糖体大亚基RNA基因的部分克隆及序列分析   总被引：1，自引：0，他引：1       下载免费PDF全文

王波  米铁柱  吕颂辉  孙军  李荣秀  于志刚 《海洋学报》2007,29(1):120-126

对7株赤潮原甲藻28S rDNA 5'端部分序列进行扩增、克隆和序列测定,并从GeneBank上获取14个原甲藻28S rDNA序列,用NJ法和ME法构建了原甲藻属的系统树,并对序列进行分析.结果表明,7株原甲藻28S rDNA扩增序列长度为950~958 bp,通过NJ法和ME法构建的系统树完全一致.大部分分离自不同海域的同种原甲藻的序列高度保守,而不同种间在序列高变区却有较大的差异.但来自南海海域的海洋原甲藻(Prorocentrum micans)与分离自其他海域的株系序列差异较大,甚至超出了有些种间的差异.由28S rDNA高变区获得的序列,有望成为浮游植物特异性分子探针设计的良好靶区域.  相似文献   

Comparative research on karyotype in three species of Arcidae     

Zheng Jiasheng  Wang Meilin  Guo Danhong  Xu Ximin  Gao Qinglan 《海洋学报(英文版)》1996,15(4):529-538

ＣｏｍｐａｒａｔｉｖｅｒｅｓｅａｒｃｈｏｎｋａｒｙｏｔｙｐｅｉｎｔｈｒｅｅｓｐｅｃｉｅｓｏｆＡｒｃｉｄａｅＺｈｅｎｇＪｉａｓｈｅｎｇ；ＷａｎｇＭｅｉｌｉｎ；ＧｕｏＤａｎｈｏｎｇ；ＸｕＸｉｍｉｎｄａｎｄＧａｏＱｉｎｇｌａｎＡｂｓｔｒａｃｔ：Ｂｙａｉｒ－...  相似文献   

耳鲍(Haliotis asinina)核糖体小亚基(18S rRNA)编码基因的克隆与序列分析   总被引：1，自引：0，他引：1       下载免费PDF全文

黄勃  方再光  刘均玲  周智  王小兵 《海洋与湖沼》2007,38(3):212-216

采用分子生物学的方法,对南海不同海区的两个地理群体耳鲍(Haliotis asinina)18S rRNA基因全长进行了克隆和序列分析,并将耳鲍18S rRNA基因的序列与NCBI数据库中已收录鲍的18SrRNA基因进行了比较。结果发现,南海耳鲍核糖体18S rRNA基因与耳鲍H.asinina isolate H11核糖体18S rRNA基因序列的相同率高达98%;同一地理群体内耳鲍核糖体18S rRNA基因序列完全一致;不同地理群体间耳鲍核糖体18S rRNA基因在碱基组成上的相似率为99%,仅在某些位点处发生了碱基替换,即腺嘌呤(T)被鸟嘌呤(G)替换;同时,将这两个不同群体中耳鲍的18S rRNA基因与泰国耳鲍18S rRNA基因序列进行比较分析发现,它们之间也只是发生了碱基替换。  相似文献   

Relationship between Shot-Induced Noise and Shot Interval in Seismic Refraction Experiments at Sea     

Herber  R.  Weigel  W.  Wong  H. K. 《Marine Geophysical Researches》1997,19(3):257-265

Seismic noise induced by the seismic source during continuous profiling reduces the signal-to-noise ratio and hence the data quality. This noise is largely dependent on the shot interval. In this paper, the noise amplitude of refraction seismic records from a special experiment is analysed as a function of the shot interval. An empirical exponential relationship between mean peak noise amplitude and shot interval is deduced. By increasing the shot interval, the induced noise can be minimized on all successive records. This results in an improvement of the data, predominantly the signal-to-noise ratio. Because the seismic signal and the shot-induced noise have nearly the same spectra, the chance of improving the signal-to-noise ratio by stacking is significantly reduced.  相似文献   

Seasonal methyltin and (3-dimethylsulphonio)propionate concentrations in leaf tissue of Spartina alterniflora of the Great Bay estuary (NH)     

James H. Weber  Marilyn R. Billings  Anne M. Falke 《Estuarine, Coastal and Shelf Science》1991,33(6)

Concentrations of total recoverable inorganic tin (TRISn), monomethyltin (MeSn³⁺), dimethyltin (Me₂Sn²⁺), trimethyltin (Me₃Sn⁺) and (3-dimethylsulphonio)propionate (DMSP) were determined in leaves of Spartina alterniflora from three sites in the Great Bay estuary (NH) from 8 May to 15 September 1989. Total methyltin concentration increased from 8·9 ng g⁻¹ (fresh weight) on 8 May to 472 ng g⁻¹ on 23 May, decreased to 52 ng g⁻¹ on 7 June and 16ng g⁻¹ on 20 June, and remained low until the last sample on 18 September. Statistical calculations showed that methyltin concentrations varied significantly with sampling week, but not with site. DMSP concentrations showed very different behaviour. During the same sampling period DMSP concentrations varied only from 7·5 to 26 μmol g⁻¹ (fresh weight). DMSP concentrations varied significantly for site, but not sampling week.  相似文献   

3种蛏类线粒体16SrRNA和COI基因片段的序列比较及其系统学初步研究   总被引：15，自引：0，他引：15  

陈丽梅  孔晓瑜  喻子牛  于珊珊  徐晖 《海洋科学》2005,29(8):27-32

对3种蛏类大竹蛏（Solen grandis），长竹蛏（Solen strictus）和小刀蛏（Cultellus attenuatus）的线粒体16SrRNA和COI基因片段序列进行了比较并对其系统学进行了初步研究。得到的序列总长度分别为472-481bp（16S）和658bp（COI）。3种蛏序列的碱基组成均显示出较高的A＋T比例（16SrRNA基因62．1％；COI基因62．8％）。对位排序比较表明，16SrRNA片段种内个体间变异较小，3种类间存在128个碱基变异位点（其中包括127个简约信息位点）和5个插入／缺失位点，总共12个碱基长；COI片段有200个碱基存在变异，其中包括191个简约信息位点，不存在任何插入／缺失位点。数据分析结果表明16SrRNA和COI基因片段大竹蛏与长竹蛏两片段的遗传距离分别为0．0856和0．1712，两竹蛏类与小刀蛏的遗传距离分别为0．3054，0．2798和0．2662，0．2933。作者认为小刀蛏与竹蛏之间的遗传距离已达到科之间的水平，结果支持将其提升为刀蛏科的分类观点。3种蛏类线粒体16SrRNA和COI基因在种间明显的多态性，证实了16SrRNA和COI基因序列均普遍适用于蛏类种及以上阶元的系统学分析。  相似文献   

赤潮叉角藻18SrDNA和ITS区序列测定与分析   总被引：20，自引：0，他引：20  

庄丽  陈月琴  李钦亮  屈良鹄 《海洋与湖沼》2001,32(2):148-154

采用PCR及克隆测序的方法，对1998年引发渤海赤潮的叉角藻18SrRNAadldey DNAITS区（Internal Transcribed Spacer Regions)进行了序列测定与分析。并通过因特网从国际分子生物学数据库中获取甲藻另外15个种的18rDNA序列，以Tetrahymena corlissi作为类群，分别采用Neighbor-Joining和Fitch方法构建甲藻较为一致和可靠的进化树图，探讨具有高度多样性和在分类上争议较多的甲藻各类群之间的形态与分子进化关系。结果表明，Prorocentrum(有2个简单的壳板）出现得较早，而大多数多甲藻目（覆盖着多个壳板）、裸甲藻目（大多数不具壳板）和膝沟藻目的成员较晚出现。另外，对叉角藻ITS区的分析表明，ITS区为高变区，是良好的分子标记，可用于叉角藻快速鉴定的专一性核酸分子探针的研制。  相似文献   

牙鲆(Paralichthys olivaceus)秦皇岛弧菌感染症及其病原细菌研究   总被引：1，自引：1，他引：1  

陈翠珍  张晓君  房海  靳晓敏  王秀云 《海洋与湖沼》2006,37(1):35-40

采用病原分离与鉴定、人工感染试验等方法,在对养殖牙鲆病例进行发病情况、病理变化特征检验的基础上,进行病原学检验。结果表明,此病例属于由弧菌属(VibrioPacini1854)细菌引起的败血感染症。经对12株纯培养菌(HQ010712-1—HQ010712-12)进行形态特征、理化特性等表观分类学指征的检验,选择代表菌株(HQ010712-1株)进行16S rRNA基因的测定与系统发育学分析等,判定为弧菌属的一个新种。将HQ010712-1株送中国典型培养物保藏中心(CCTCC)进行了复核鉴定与分类定名,依据分离地定名为秦皇岛弧菌(Vibrioqinhuangdaorasp.nov.);参考菌株为HQ010712-1。  相似文献   

Genetic relationship between Neobenedenia girellae and N. melleni inferred from 28S rRNA sequences     

WANG Jun  ZHANG Wen  SU Yongquan  DING Shaoxiong 《海洋学报(英文版)》2004,23(4):709-716

The fragments of 350 bp in 28S rRNA from the closely related monogenea of trematoda, Neobenedenia girellae and N. melleni are obtained by polymerase chain reaction (PCR) amplified using a couple of special primers and then sequenced. The results show that the comparison of 28S rRNA sequences, with only a base varying in 337 bp accounting for 0.3% genetic difference, from the relative species N. girellae and N. melleni parasitized on the different fishes in different farms displays that they possess a very high genetic similarity of 99.7%, higher than that of 99.41% for the single species N. melleni sampled in different areas, and the intraspecific divergence of N.melleni is 0.59%. Meanwhile, the interspecific differences between the two Neobenedenia and three Benedenia (i.e., B. lutjani, B. rohdei and B. seriolae) range from 2.08% tol 1.73%. In addition, UPGMA and MP molecular phylogenetic trees are constructed and proved to be consistent with each other. Though the morphological characteristics and the results of genetic diversity for the two Neobenedenia show a high similarity, whether they belong to a single species or not are still undefined, and the more genes of them should be further investigated, in combination with the systematical and detailed morphological study.  相似文献