Genetic variability of half-smooth tongue sole Cynoglossus semilaevis populations using microsatellite markers     

LIU Yunguo  GUO Yinghui  LIU Shuaishuai  LI Junfeng  YE Naihao 《海洋学报(英文版)》2011,30(3):76-83

Ten highly variable microsatellite loci were performed in order to evaluate the genetic variability of wild and hatchery samples of half-smooth tongue sole Cynoglossus semilaevis. A group of 200 genotypes belonging to four wild samples, Laizhou (LZ), Weihai (WH), Qingdao (QD), Rizhao (RZ) and one hatchery sample, Mingbo (MB), were screened. All of the ten microsatellite loci screened in this study showed marked polymorphism. A total of 70 different alleles were observed over all loci. The number of alleles per locus ranged from 4.2 to 10.6. The average of expected and observed heterozygosity ranged from 0.67 to 0.82, and from 0.77 to 0.87, respectively. A total of 10, 16, 10, 11 and 5 unique alleles each were found in LZ, WH, QD, RZ and MB populations. The effective number of alleles varied from 2.87 for HSTS_7 to 6.83 for HSTS-h. The number of average genotypes ranged from 6.0 for HSTS_a to 15.4 for HSTS_h. As compared with the wild populations, the hatchery population, MB, showed significant genetic changes such as fewer alleles per locus (P <0.05), a smaller number of low frequency alleles (P <0.05), a small number of unique alleles and a small number of genotypes (P <0.05), all indicative of a reduction in genetic diversity.  相似文献   

柴北缘含煤区地层测井物性特征分析     

潘语录 《中国煤炭地质》2011,(12):42-45

以柴达木盆地北缘的鱼卡煤田为例,对含煤地层中的各种岩性的测井响应特征进行分析,认为该区的地球物理特征属＂简单易区别＂型,即煤岩层的物性差异较大,物性条件较好,不同岩性在伽马-伽马、自然伽马、电阻率等测井曲线上的特征明显,根据曲线的变化幅度、峰值高低等曲线特征,可有效进行煤岩层划分与对比。根据实际测井解释成果,对侏罗系上统红水沟组、采石岭组、侏罗系中-下统大煤沟组的页岩段、上含煤段及下含煤段等标志层段的测井曲线组合特征进行了描述。  相似文献   

文昌鱼线粒体基因组特征分析及分子标记探讨     

申欣  田美  孟学平  程汉良 《海洋科学》2011,35(7):7-13

线粒体基因组已被广泛应用于后生动物分子系统发育和群体遗传的研究。文昌鱼（Amphioxus）作为研究脊椎动物起源和进化的模式动物,在脊椎动物起源和进化研究中占据极为重要的位置。作者综合分析文昌鱼2科7个种的51条线粒体基因组全序列,全面揭示了文昌鱼线粒体基因组的基本特征。文昌鱼线粒体基因组均编码后生动物标准的37个基因...  相似文献   

利用贝壳颜色标记分析皱纹盘鲍苗种繁育中的无意识选择     

李加琦  刘晓 《海洋科学》2011,35(10):22-26

利用皱纹盘跑（Haliotis discus hannai）贝壳颜色与摄食饵料相关且贝壳生成后色调不再改变等特点,以幼鲍贝壳顶部摄食底栖硅藻期间形成的褐红色部位的壳长指示它剥离时的壳长,将贝壳颜色用作形态标记,首次在皱纹盘鲍苗种繁育中观察到无意识选择。在投喂人工配合饵料后的第3天与第30天,分别从遗传背景不同的8组样本...  相似文献   

应用纤维素示踪北京市PM_2.5天然植被排放来源的研究     

丁毅  张烃  董树屏  刘咸德  梁汉东 《岩矿测试》2013,32(5):738-746

大气细粒子(PM_2.5)污染是全球尤其是我国许多城市的重要环境问题。利用示踪物质识别和估算大气颗粒物来源是公认的可靠技术,纤维素可以作为示踪物质表征一次颗粒物的天然植被排放来源。本文针对大气细粒子中纤维素含量低、全程序空白相对较高等难点,优化建立了纤维素酶水解、GOD-苯酚-四氨基安替比林测糖法,在我国首次应用于测定大气PM_2.5中纤维素的含量,估算天然植被排放源的贡献。方法检出限为0.26 μg/m³(纤维素),可以满足大气PM_2.5纤维素测定要求;而且有效地降低了空白,全程序空白值(36.5 μg葡萄糖)低于文献方法空白值(53.8 μg葡萄糖),使之更适合于PM_2.5的测定。使用本方法对2012年5月至6月采集的北京市大气PM_2.5样品进行分析,纤维素检出率为96%,纤维素的平均含量为(0.573±0.17) μg/m³,折合为天然植被排放量占PM_2.5质量浓度的1.37%±0.65%;天然植被排放源对有机碳的平均贡献率为4.4%,最大达到9.2%,反映出天然植被排放是北京市PM_2.5的重要来源之 一。本研究方法为我国城市大气颗粒物(包括总悬浮颗粒物、PM₁₀、PM_2.5等)来源识别提供了新的手段。  相似文献   

测井曲线在杭东勘查区煤炭普查煤岩层对比中的应用     

王开来  王琛  刘付光 《中国煤炭地质》2013,(12):68-71

杭东普查区煤层产状平缓,构造简单,但由于含煤地层为陆相沉积,煤层层数多、厚度、间距变化大,沉积岩相变化快、大范围内缺乏稳定的对比标志层,因此煤岩层对比是本区勘查需要重点解决的难题。通过对比杭东普查区与邻区200余孔的测井资料,分析煤岩层在各种测井曲线的异常特征与异常组合规律,确定了对煤岩层对比有重大意义的标志层,如延长组与上覆延安组的典型视电阻率异常分界;延安组在高视电阻率曲线上的“树杆”状凸起特征;安定组视电阻率曲线近直线的低值形态;4-1煤在视电阻率曲线上呈现出的“斜坡”状或“刀”状高异常,以及在自然伽玛曲线上的“凹坑”特征;侏罗系中统直罗组的高伽马异常,等等。这些典型特征保证了该区煤岩层对比可靠性,为提交优质地质勘查报告发挥了重要作用。  相似文献   

五河县大巩山地区褶皱构造浅析     

张家嘉  罗明光 《安徽地质》2013,(4):253-255

大巩山西蝈堆组蚌埠期褶皱可利用含白云质大理岩透镜体的蛇纹岩作为标志层来恢复,推测其为轴面倒向北的紧密同斜褶皱,S0近南北向,枢纽西倾,地层由东向西变新。  相似文献   

100hPa高压环流和东风气流的季节、年际和年代际变化   总被引：12，自引：2，他引：12       下载免费PDF全文

徐忠峰  钱永甫 《热带气象学报》2003,19(3):225-233

利用NCEP／NCAR再分析资料，分析了中低纬地区100hPa高压环流和东风气流的季节、年际和年代际变化，并与南亚高压的相应变化进行对比分析。结果表明，100hPa高压环流中心有明显的季节分布特征，其中心经度的主频区与100hPa南亚高压中心经度的主频区位置较为一致。南亚高压和东风气流的部分特征参数有明显的年际和年代际变化。南亚高压参数有明显的10年和大于20年的振荡周期。东风气流的面积有2～5年的振荡周期，其强度则有4年和15年的振荡周期。  相似文献   

大兴安岭中段木瑞组的发现及其地质意义     

李望东  聂立军  王聪  周晓东 《吉林地质》2013,(4):11-12

在内蒙古1：5万蘑菇山幅、柴河源幅、苏河屯幅、兴安幅四幅区域地质调查中,研究人员通过详细的野外地质调查,首次在中侏罗统满克头鄂博组下部发现木瑞组,二者为整合接触关系。该组岩石类型为紫红色泥质粉砂岩夹灰白色砾岩,出露不完整,从而证明大兴安岭中段在中侏罗世历经陆相山间盆地堆积,具快速堆积特点。该组可作为一个区域性的标志层,对研究大兴安岭中段在中侏罗世期间地质演化史具有重要意义。  相似文献   

Fine particulate organic matter (FPOM) transport and processing in littoral interstices – use of fluorescent markers     

Günter Gunkel  Camilla Beulker  Anja Hoffmann  Jan Kosmol 《Limnologica》2009,39(3):185-199

A fluorescent labelling method is presented as a new tool for the investigation of organic particle transport and biogenic carbon cycling processes in sandy littoral interstices at Lake Tegel, Berlin, Germany. Passive particle transport through the pore system was studied by in situ exposition of 2.4 μm monodisperse polymeric resin microparticles stained with 7-amino-4-methylcoumarin (AMC). Uptake of fluorescein-5-isothiocyanate (FITC)-labelled Chlorella vulgaris and fine particulate organic matter (FPOM) by the interstitial fauna was investigated in laboratory and field experiments. The major portion (>85%) of the FITC-labelled particles added to sediment cores was recovered from the topmost centimetre of sediment during the study period of 14 days. Uptake of FITC-labelled FPOM was observed in several benthic groups, e.g. chironomids, microcrustaceans, oligochaetes and tardigrads, whereas C. vulgaris was ingested by oligochaetes only. There is evidence to suggest that both are suitable materials for investigating the fragmentation and ingestion of organic material by herbivorous and detritivorous fauna. Field experiments with inert microparticles and FITC-labelled FPOM (<1 mm) prepared from dried alder leaves were carried out in plexiglass tubes as in situ whole core technique. Within the investigation period of two weeks, the transport of FPOM was restricted to the topmost 2–3 cm of sediment in conjunction with a distinct fragmentation to finer size classes with respect to increasing sediment depth. Vertical FPOM transport was hindered by a high interstitial concentration of natural POM and an intensive settlement of the interstices by algae (mainly epispammic algae, 65–96% of algae cell number) and extra-cellular polymeric substances (EPS), which formed a dense three-dimensional structure.  相似文献