重金属胁迫对两种海洋饵料微藻的急性毒性效应研究     

于小娣  师玥  刘泳  周斌  王其翔  张鑫鑫  唐学玺  王悠 《中国海洋大学学报(自然科学版)》2014,44(2)

在模拟生态环境条件下研究了铜(Cu2+)、镉(Cd2+)和锌(Zn2+)3种重金属离子对2种海洋饵料微藻(青岛大扁藻(Platymonas helgolandica)和等鞭金藻8701(Isochrysis galbana 8701))的急性毒性效应,分析其对种群增长的影响,并利用透射电镜对微藻亚显微结构的变化进行分析。在实验浓度范围内,2种微藻的种群增长对3种重金属离子的急性胁迫表现出类似的变化趋势,随着胁迫浓度的增加,微藻的相对增长率均呈现出不断下降的趋势;通过比较2种微藻的48hEC50和96h-EC50发现:3种重金属离子的毒性程度为Cu2+Cd2+Zn2+,等鞭金藻8701对重金属胁迫的敏感性高于青岛大扁藻。2种微藻的亚显微结构观察显示,二者的膜结构及细胞核对Cu2+胁迫并不太敏感;与对照组相比等鞭金藻8701的色素体及贮能物质受影响较严重,而青岛大扁藻的叶绿体及淀粉粒受影响较大。  相似文献   

一株海洋微藻的分离鉴定及其产油条件优化     

王亚洁  赵阳国  白洁  师振华  王俊彩 《中国海洋大学学报(自然科学版)》2014,44(2)

自青岛近岸海水中分离到一株硅藻,经形态学和分子生物学鉴定,表明该藻与小头菱形藻(Nitzschia microcephala)最为相近,18SrDNA相似性99%以上。研究以f/2培养基为基础,对该藻的生长和油脂积累条件进行探析,表明其在pH=6~8时具有较高的生长速率,最大可达到0.01d-1,油脂含量随pH的升高而降低;添加SiO2-3可以抑制菱形藻的生长和油脂积累;高浓度的Fe3+能促进菱形藻的生长;在Zn2+浓度为1.74μmol/L的条件下,菱形藻能够较好地生长并且进行油脂的积累;其中pH、SiO2-3和Fe3+是影响该藻生长和油脂积累的主要因子。正交试验研究表明,该藻在f/2培养液中生长和油脂积累的最优条件为初始pH=9,SiO2-3和Fe3+添加浓度分别为0.07和100μmol/L。在该最优条件下,该藻的生物量和油脂积累可分别达到94.7mg/L和19.6%。  相似文献   

Measurement of trace nitrate concentrations in seawater by ion chromatography with valve switching     

杜娟  法芸  郑岳  李学兵  杜芳林  杨海燕 《中国海洋湖沼学报》2014,32(3):732-736

An ion chromatographic method with a valve switching facility was developed to determine trace nitrate concentrations in seawater using two pumps, two different suppressors, and two columns. A carbohydrate membrane desalter was used to reduce the high concentrations of sodium salts in samples. In this method, trace nitrate was eluted from the concentrator column to the analytical columns, while the matrix flowed to waste. Neither chemical pre-treatment nor sample dilution was required. In the optimized separation conditions, the method showed good linearity （R〉0,99） in the 0.05 and 50 mg/L concentration range, and satisfactory repeatability （RSD〈5%, n=6）. The limit of detection for nitrate was 0.02 mg/L. Results showed that the valve switching system was suitable and practical for the determination of trace nitrate in seawater.  相似文献   

羟基磷灰石对水溶液中Fe³⁺的吸附动力学及热力学研究     

周珍旭  钱功明  李道  王发杰 《岩石矿物学杂志》2014,33(6):1157-1160

研究了羟基磷灰石(HAP)对水溶液中Fe3+的吸附动力学及热力学。研究表明,HAP对水溶液中Fe3+的吸附符合Langmiur等温吸附,ct/q=0.006 4 ct+0.018 3;该吸附反应符合一级反应动力学方程,ln CR=-0.043 5 t+4.324 4;吸附反应活化能为Ea=36.26 k J/mol;标准摩尔反应焓为正值表明反应过程吸热;当温度大于285.7 K时,吉布斯自由能小于0,反应可自发进行。  相似文献   

Geochemical and isotopic evidence of a groundwater source in the Corral Canyon meadow complex,central Nevada,USA     

Eliot A. Atekwana  Dorothea S. Richardson 《水文研究》2004,18(15):2801-2815

Major inorganic ions and stable carbon and oxygen isotopes in stream water, groundwater, groundwater seeps and springs were measured in the Corral Canyon meadow complex and watershed in the Toquima Mountains of central Nevada, USA. The purpose of the study was to determine whether stream water or groundwater was the source of water that supports vegetation in the meadow complex. Water samples from the watershed and meadow complex were mixed cation–HCO₃ type. Stream water sampled at different locations in the meadow complex showed variations in temperature, pH and specific conductance. The cation–anion proportions for stream water were similar to groundwater, groundwater seeps and runoff from the meadow complex. Stable oxygen isotope ratios for stream water (?17·1 to ?17·6‰ versus VSMOW) and groundwater and groundwater seeps in the meadow site (?17·0 to ?17·7‰ versus VSMOW) were similar, and consistent with a local meteoric origin. Dissolved inorganic carbon (DIC) and the δ ¹³C_DIC for stream water (?12·1 to ?15·0‰ versus VPDB) were different from that of groundwater from the meadow complex (?15·3 to ?19·9‰ versus VPDB), suggesting different carbon evolution pathways. However, a simple model based on cation–δ ¹³C_DIC suggests that stream water was being recharged by shallow groundwater, groundwater seeps and runoff from the meadow complex. This leads to the conclusion that the source of water that supports vegetation in the meadow complex was primarily groundwater. The results of this study suggest that multiple chemical and stable carbon isotope tracers are useful in determining the source of water that supports vegetation in meadow complexes in small alpine watersheds. Copyright © 2004 John Wiley & Sons, Ltd.  相似文献   

EFFECTS OF COMPOSITION AND STRUCTURE OF ALGINATES ON ADSORPTION OF DIVALENT METALS     

郑乃余  张燕霞  范晓  韩丽君 《中国海洋湖沼学报》1994,(1)

Results of a series of experiments(on the adsorption of divalent metal ions by dried alginic acid, Na and Ca alginates of different composition and block structure) conducted in this systematic study of the effects of the composition and structure of alginates on the static adsorption equilibrium of divalent metal ions indicate that the properties of alginate adsorption to divalent metal ions are highly different, depending not only on the cations used, but also on the form and structure of the alginates. There is close correlation between the adsorption properties and the structure of the alginates. The selectivity coefficient of Na alginate for Cd-Sr ion exchange tends to increase with the increase of the M/G ratio in alginate, whereas the adsorption capacity of Ca alginate for Cu~(2+) ion decrease with the increase of the G-block or the average length of the G-block ((?)_G) and the total adsorption capacity of alginic acid is found to vary in the same order as the F_(MM)(diad frequency) in alginate in  相似文献   

常州市地下水化学特征与成因分析     

苏东  龚绪龙  杨磊  黄敬军  许书刚  龚亚兵  崔龙玉 《地质论评》2023,69(3):1039-1049

常州市是长江三角洲地区典型的工业化城市,多年来的快速发展对地下水环境产生了系列影响,地下水化学组分受到天然条件和人为活动的双重因素控制,现状地下水化学成因和影响因素亟需深入研究。本文在地下水赋存条件分析的基础上,综合采用统计分析、离子比值、主成分分析法对常州各层地下水化学特征和成因开展分析。结果表明,区域潜水水化学类型以HCO₃^-—Ca²⁺·Mg²⁺、HCO₃^-—Ca²⁺和HCO₃^-·Cl^-—Na⁺·Ca²⁺型为主,第Ⅰ承压水以HCO₃^-—Na⁺·Ca²⁺和HCO₃^-—Ca²⁺型为主、第Ⅱ承压水以HCO₃^-—Na⁺  相似文献   

济南四大泉群水化学特征及其成因分析     

毛玉洁 《地质与勘探》2022,58(5):1042-1056

为明晰济南四大泉群水化学动态变化特征及成因,利用离子比例系数、PHREEQC模拟以及氢氧同位素反演等方法分析各泉群水化学成分特征及其形成过程。研究表明：（1）四大泉群阳离子Ca2+为优势离子,阴离子HCO3-为优势离子;（2）潭西泉和珍珠泉水化学类型稳定,而趵突泉和黑虎泉由HCO3—Ca型变为HCO3·SO4—Ca型;（3）四大泉群受地表水、寒武系凤山组-奥陶系岩溶水以及寒武系张夏组岩溶水混合补给,其中趵突泉、黑虎泉接受寒武系凤山组-奥陶系岩溶水补给占比略大,而潭西泉、珍珠泉接受寒武系张夏组岩溶水补给占比略大;黑虎泉滞留时间最短,循环交替强烈;（4）水岩相互作用强弱程度导致泉群水化学成分差异,溶解沉淀作用强度整体大于阳离子交换作用。趵突泉阳离子交换作用强度最弱,黑虎泉伴随有去白云化作用,潭西泉和珍珠泉水化学作用及强度类似,进一步印证潭西泉和珍珠泉有相同的补给来源及运移机制。该研究成果对水资源合理利用和岩溶大泉的保护具有重要意义。  相似文献   

WATER　RESOURCES　TRANSFORMATION　AND　WATER　QUALITY　VARIATION　IN　THE　URUMQI　RIVER　BASIN     

曲耀光  骆鸿珍 《中国地理科学(英文版)》1995,5(4):325-335

ＷＡＴＥＲＲＥＳＯＵＲＣＥＳＴＲＡＮＳＦＯＲＭＡＴＩＯＮＡＮＤＷＡＴＥＲＱＵＡＬＩＴＹＶＡＲＩＡＴＩＯＮＩＮＴＨＥＵＲＵＭＱＩＲＩＶＥＲＢＡＳＩＮＱｕＹａｏｇｕａｎｇ（曲耀光）；ＬｕｏＨｏｎｇｚｈｅｎ（骆鸿珍）（ＬａｎｚｈｏｕＩｎｓｔｉｔｕｔｅｏｆＧ...  相似文献   

Propagation of Extremely Low Frequency Waves Through the Ionosphere     

Singh  Ashok K.  Narayan  D.  Singh  R. P. 《Earth, Moon, and Planets》2002,91(3):161-179

The propagation features of extremely low frequency electromagnetic waves through the multicomponent ionospheric plasma are studied. It is shown that at relatively lower frequencies refractive index for right hand mode is higher than the left-hand mode, which is reversed at higher frequencies. The thermal temperature of plasma particle causes decrease in phase and group velocities of both right and left-hand modes. The crossover frequencies for different plasma models are computed and variation with ion concentration and thermal velocity is studied. Explicit expression for group velocity and travel time has been derived and studied numerically. Finally, we have presented simulation of the ion whistler spectrograms for Hydrogen, Helium and Oxygen ions present in the ionospheric plasma. The results are compared with the experimentally detected hydrogen and helium ion whistlers. The importance of the present study in the exploration of ionospheric plasma is illustrated.  相似文献