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1.
Black rockfi sh Sebastes schlegelii juveniles(30–40 mm total length) were immersed in a range of calcein(CAL) solutions at concentrations ranging from 50 to 250 mg/L and alizarin red S(ARS) solutions at concentrations ranging from 100 to 500 mg/L in fi ltered seawater(salinity 30) for 24 h. Fluorescent marks were detected in otoliths(sagittae, asteriscus), scales, fi n rays(dorsal, pectoral, ventral, anal, and caudal fi n rays), and fi n spines(dorsal, ventral, and anal fi n spines) after a 60-d growth experiment. With the exception of 50–100 mg/L CAL, acceptable marks were produced in the otoliths and fi n spines by all concentrations of CAL and ARS. In particular, marks were clearly visible under normal light in the sagittae, asteriscus, and fi n spines of fi sh immersed in 200–500 mg/L, 300–500 mg/L, and 200–500 mg/L ARS, respectively. Scales and fi n rays had acceptable marks at much higher concentrations(≥50 mg/L CAL, ≥300 mg/L ARS for scales and ≥50 mg/L CAL, ≥200 mg/L ARS for fi n rays). The mark quality was highest(i.e., acceptable marks were observed in all sampled structures after immersion marking) in fi sh immersed in 150–250 mg/L CAL or 300–500 mg/L ARS. In addition, there was no signifi cant difference in survival and growth of marked fi sh compared with controls 60 d post-marking( P 0.05).  相似文献   

2.
Molecularly imprinted polymers(MIPs) were prepared by the bulk polymerization using crystal violet as the template molecule, and the methacrylic acid and ethylene glycol dimetheacrylate as functional monomer and cross-linker, respectively. Systematic investigations of synthetic conditions were conducted. The surface morphology and recognition mechanism of the obtained polymers were studied using scanning electron microscope and spectrophotometric analysis. MIPs showed high affinity to template molecule and were successfully applied as special solid-phase extraction sorbent for selective extraction of crystal violet from natural seawater. An off-line molecularly imprinted solid-phase extraction(MISPE) method followed by high-performance liquid chromatography with diodearray detection for the analysis of crystal violet was also established. MISPE columns have good recoveries for crystal violet standard solutions and good linearity was obtained over the concentration range of 0-200 μg L-1(R2 0.99). Finally, two natural seawater samples were investigated. The recoveries of spiked seawater on the MISPE columns were from 44.47% to 62.34%, the relative standard deviation(n=3) being in the range of 2.89%-5.96%.  相似文献   

3.
Fe^2＋ acted as an accessorial factor for many cellular enzymatic reactions is very important for seaweed growth and development, but the Fe^2＋ requirement in nori had not been seen. Porphyra yezoensis cells were separated enzymatically and cultured in a series of sterilized seawater media containing various concentrations of Fe^2＋. The growth development and cell were investigated in this work. Through this experiment, two biologically-meant concentration scales were found, one is low concentrations, 12.1-102.1μg/L, 10-100 times than that in seawater, favoring the development of isolated cells of Porphyra and the other was high concentrations, more than 10mg/L inhibiting the cell growth, leading to the deformity and shrinkage of the cells. At the concentration of 50 mg/L, the cells stopped growing and died eventually.  相似文献   

4.
A novel method for on-site determination of trace iron was developed using membrane preconcentration and spectrophotometric detection. Fe(Ⅱ)-ferrozine complex was reacted with cetyltrimethylammonium bromide (CTAB) to form a Fe(Ⅱ)-ferrozine-CTAB paired compound, which was collected on a membrane by filtration under vacuum. The membrane was immersed in 2 mL of ethanol-nitric acid and the absorbance of the solution measured for quantitative analysis. Various factors affecting the iron collection and determination were investigated. With different sample preconcentration volumes, the range of determination was broadened to 0.5-120 g/L. The detection limit of this method reached 0.19 g/L and the recoveries were between 97.2 and 109% when the concentration enrichment was about 45. The relative standard deviation (n = 7) was 1.9% for samples containing 10 g/L Fe. Twelve seawater samples were analyzed on-site using the proposed method, and two were also analyzed in inductively coupled plasma mass spectrometry. No significant difference was shown between the two methods by the Student’s t-test. The method has also been used on-site for iron enrichment experiments with phytoplankton and concluded to be simple, accurate and inexpensive.  相似文献   

5.
Many aquatic organisms are negatively affected by exposure to high copper concentrations.We investigated the biochemical response of the mussel Mytilus coruscus(Mytiloida:Mytilidae) to copper exposure.In vivo bioassays using M.coruscus and different copper concentrations were conducted.The activity of six biomarkers,namely superoxide dismutase(SOD),catalase(CAT),acid phosphatase(ACP),alkaline phosphatase(AKP),glutamic-oxaloacetic transaminase(GOT) and glutamic-pyruvic transaminase(GPT) were measured.Survival rates decreased with increased copper concentrations and exposure times.The LC50 values at 48,72,and 96 h exposure were 0.48,0.37,and 0.32 mg/L,respectively.Within digestive glands,CAT activity increased with increasing Cu concentrations.The activity of AKP showed no significant change,while the remaining four enzymes showed decreasing activity with increasing Cu concentrations.Within the gills,AKP activity increased when the Cu concentration was 0.05 mg/L,but showed no significant changes at higher concentrations.Activity of CAT and ACP within gills tended to decrease with increasing Cu concentration.The activity of SOD and GPT decreased at an exposure concentration of 0.2 mg/L.GOT activity within gills decreased at 0.1 mg/L and increased at an exposure concentration of 0.2 mg/L.Within the adductor muscle,AKP activity increased at 0.05 mg/L but did not change at higher exposure concentrations.ACP activity within adductor muscle tissue showed no change,while activities of CAT,GOT and GPT decreased with increasing Cu concentrations.SOD activity within the adductor muscle tissue significantly decreased at the 0.02,0.05 and 0.2 mg/L exposure concentrations.Our results show tissue specific differences for the six biomarkers in for M.coruscus.Our findings provide the basis for the establishment of reference activity levels against which biomarker changes can be estimated,and are essential preliminary steps in development of in vivo bioassays.  相似文献   

6.
徐闻珊瑚礁保护区营养盐时空分布特征   总被引：2，自引：1，他引：1  
2006年8月(夏季)、2006年11月(秋季)、2007年2月(冬季)和2007年4月(春季)在徐闻珊瑚礁自然保护区灯楼角至流沙湾近岸海域调查徐闻珊瑚礁保护区的营养盐变化及空间分布特征。结果表明:徐闻珊瑚礁保护区水域溶解态无机氮以NO3--N为主,其含量超过总溶解无机氮的50%;各站点NO2--N含量相对较低,冬季NO3--N和NH4+-N含量均高于其它季节;无机磷含量在0mg/L～0.030mg/L之间变化;活性硅含量表现为夏秋季节高、冬春季节低;表层水体硝酸盐氮/无机磷原子比值(N/P)夏季较低,不存在无机磷受限情况,而冬季N/P整体较高,此时水体主要受无机磷限制。  相似文献   

7.
The capability ofScenedesmus obliquus to remove cations （K^＋, Na^＋, Ca^2＋, Mg^2＋） from saline- alkaline water was investigated at different salinities （0, 5, 10, 15, 20, 25） and carbonate alkalinities （0, 5, 10, 15, 20, 25, 30, 35 mmol/L）. K^＋, Na^＋, Ca^2＋, and Mg^2＋ in saline-alkaline water were efficiently removed by S. obliquus. The maximum removal of the cations （29.37 mg for K^＋, 185.85 mg for Na^＋, 23.07 mg for Ca^2＋, 66.14 mg for Mg^2＋） occurred at salinity 25. The maximum removal of K^＋ （2.28 mg）, Na＋ （6.62 mg）, Ca^2＋ （1.01 mg）, and Mg2＋ （0.62 mg） occurred at carbonate alkalinities of 25 mmol/L for K＊, 35 mmol/L for Na＋, 20 mmol/L for Ca2＋, and 25 mmol/L for Mf＋, respectively. Under a salinity stress, the concentration of Na＇ in S. obliquus increased significantly, while that of K~ decreased significantly. The concentrations of Ca^2＋ and Mg2＋ decreased as well. The ratios of K＋/Na~, Ca2＋/Na^＋, and Mg^2＋/Na^＋ were significantly lower in all salinity treatments than those of the control. Under alkaline stress, the concentrations of Nan and K＋ in S. obliquus decreased significantly and the ratios of K^＋/Na^＋, Ca2＋/Na^＋, and Mg^2＋/Na^＋ were significantly higher in all treatments than in the control. Moreover, the concentrations of Ca2＋ and Mg2＋ in S. obliquus at alkalinities of 5-10 mmol/L were significantly higher than those of the other treatments. The removal of Na＋ by S. obliquus mainly occurs through biosorption, and Mg^2＋ and Ca^2 ＋ were removed through both biosorption and bioaccumulation.  相似文献   

8.
The Henry’s Law constant (k) for phosphine in seawater was determined by multiple phase equilibration combined with headspace gas chromatography. The effects of pH, temperature, and salinity on k were studied. The k value for phosphine in natural seawater was 6.415 at room temperature (approximately 23°C). This value increases with increases in temperature and salinity, but no obvious change was observed at different pH levels. At the same temperature, there was no significant difference between the k for phosphine in natural seawater and that in artificial seawater. This implies that temperature and salinity are major determining factors for k in marine environment. Double linear regression with Henry’s Law constants for phosphine as a function of temperature and salinity confirmed our observations. These results provide a basis for the measurement of trace phosphine concentrations in seawater, and will be helpful for future research on the status of phosphine in the oceanic biogeochemical cycle of phosphorus.  相似文献   

9.
A method is described for determining dimethylI sulfide (DMS) in seawater. DMS was first extractedfrom the seawater using organic reagent, then reverse-extracted by 5% HgCl2. In the laboratory DMS wasreleased by concentrated HCI and finally measured by GC-FPD. The limit of detection me O.05 ng ofS. Measurements of DMS along surface transects and on wtital profibe across the EaSt China Sca (Ers)continental sheif showed tha itS conodIations of S in the surface seawater ranged from 64-180 ng/L andthat itS vertical djstribuion was divided into 3 trpes. Model talculations of a stagnant film show a DMSflux of 10.6 umol/m_2d the air-sea inteIha.  相似文献   

10.
Dimethylsulfide in the South China Sea   总被引：1，自引：0，他引：1  
ＩＮＴＲＯＤＵＣＴＩＯＮＧｒｅａｔｅｆｆｏｒｔｓｗｅｒｅｄｅｖｏｔｅｄｒｅｃｅｎｔｌｙｔｏｓｔｕｄｙｉｎｇｄｉｍｅｔｈｙｌｓｕｌｆｉｄｅ(ＤＭＳ)ｄｉｓｔｒｉｂｕｔｉｏｎｉｎｓｅａｗａｔｅｒ,ａｓｉｔａｃｃｏｕｎｔｓｆｏｒｔｈｅｍａｊｏｒｐａｒｔｏｆｔｈｅｓｕｌｆｕｒｆｌｕｘｆｒｏｍｔｈｅｏｃｅａｎｓｔｏｔｈｅａｔｍｏｓｐｈｅｒｅ.Ｍｏｒｅｏｖｅｒ,ｉｔｓｏｘｉｄａｔｉｏｎｐｒｏｄｕｃｔｓｉｎｔｈｅａｔｍｏｓｐｈｅｒｅｍａｙｉｎｆｌｕｅｎｃｅｅｎｖｉｒｏｎｍｅｎｔａｌａｃｉｄｉｆｉｃａｔｉｏｎａｎｄ…  相似文献   

11.
Concentrations of the trace metals Cu,Cd,Pb,and Zn were measured in seawater,rhizosphere sediments,interstitial water,and the tissues of three tropical species of seagrasses (Thalassia hemprichii,Enhalus acoroides and Cymodocea rotundata) from Xincun Bay of Hainan Island,South China.We analyzed different environmental compartments and the highest concentrations of Pb and Zn were found in the interstitial and seawater.The concentrations of Cd and Zn were significantly higher in blades compared with roots or rhizomes in T.hemprichii and E.acoroides,respectively.A metal pollution index (MPI) demonstrated that sediment,interstitial water,and seagrasses in the sites located nearest anthropogenic sources of pollution had the most abundant metal concentrations.There was obvious seasonal variation of these metals in the three seagrasses with higher concentrations of Cu,Pb and Zn in January and Cd in July.Furthermore,the relationships between metal concentrations in seagrasses and environmental compartments were positively correlated significantly.The bioconcentration factors (BCF) demonstrated that Cd from the tissues of the three seagrasses might be absorbed from the sediment by the roots.However,for C.rotundata,Zn is likely to be derived from the seawater through its blades.Therefore,the blades of T.hemprichii,E.acoroides and C.rotundata are potential bio-indicators to Cd content in sediment,and additionally Zn content (C.rotundata only) in seawater.  相似文献   

12.
Measurements of ammonium and nitrate uptakes by natural phytoplankton assemblages from Jiaozhou Bay at various combinations of ammonium and nitrate concentrations with 15N trace techniques showed that uptake rate of either nitrogen was influenced by the presence of the other but that the influence of ammonium on nitrate uptake was much greater than, that of nitrate on ammonium uptake. The influence mechanism of ammonium on nitrate uptake manifested as competition at lower concentrations and as inhibition at higher concentrations (ammonium concentration >0.6 umol/L), but no total inhibition appeared within the concentration.range of the experiments (0-10umol/L). The influence of nitrate on ammonium uptake seems to be a result of competition for uptake sites on the cell surface. In view of the in situ nutrient concentration in the given marine . environment, it is believed that both nitrogen sources are utilized by phytoplankton. Nitrate uptake in the presence of ammonium and ammonium uptake in the presen  相似文献   

13.
Gracilaria asiatica,being highly efficient in nutrient absorption,is cultivated in sea cucumber ponds to remove nutrients such as nitrogen and phosphate.It was cultured in a laboratory simulating field conditions,and its nutrient absorption was measured to evaluate effects of environmental conditions.Ammonia nitrogen(AN),nitrate nitrogen(NN),total inorganic nitrogen(TIN),and soluble reactive phosphorus(SRP) uptake rate and removal efficiency were determined in a 4×2 factorial design experiment in water temperatures(T) at 15℃ and 25℃,algae biomass(AB) at 0.5 g/L and 1.0 g/L,total inorganic nitrogen(TIN) at 30 μmol/L and 60 μmol/L,and soluble reactive phosphorus(SRP) at 3 and 6 μmol/L.AB and ambient TIN or SRP levels significantly affected uptake rate and removal efficiency of AN,NN,TIN,and SRP(P<0.001).G.asiatica in AB of 0.5 g/L showed higher uptake rate and lower removal efficiency relative to that with AB of 1.0 g/L.Nitrogen and phosphorus uptake rate rose with increasing ambient nutrient concentrations;nutrient removal efficiency decreased at higher environmental nutrient concentrations.The algae preferred to absorb AN to NN.Uptake rates of AN,NN,and SRP were significantly affected by temperature(P<0.001);uptake rate was higher for the 25℃ group than for the 15℃ group at the initial experiment stage.Only the removal efficiency of AN and SRP showed a significant difference between the two temperature groups(P<0.01).The four factors had significant interactive effects on absorption of N and P,implying that G.asiatica has great bioremedial potential in sea cucumber culture ponds.  相似文献   

14.
于2008年3月至2009年1月用电感耦合等离子体质谱法(ICP-MS)对湛江港海水中的铜(Cu)、锌(Zn)、铅(Pb)、镉(Cd)进行调查。分析结果表明:2008年湛江港湾海水中Cu、Zn、Pb、Cd含量较低,年平均含量分别为4.46×10-3mg/L、14.2×10-3mg/L、0.795×10-3mg/L、0.588×10-3mg/L,总体上表层海水中Cu、Zn、Pb、Cd的含量均略低于底层海水。各季度海水中重金属含量的比较结果为:Cu的含量秋季>春季>夏季>冬季,Zn的含量夏季>春季>秋季>冬季,Pb的含量秋季>夏季>春季>冬季,Cd的含量冬季>夏季>秋季>春季。湛江港湾的Cu、Zn、Pb、Cd的含量均在中部水域湛江港码头核心作业区最高,港湾外较低;Zn、Cd在港湾内的含量较高。按照单因子污染指数法评价,湛江港湾海水中的Cu、Zn、Pb、Cd含量均低于国家二类海水水质标准规定的限值,水质较好。  相似文献   

15.
A study on the nonspecific immunity of Litopenaeus vannamei ever inhabiting freshwater and seawater was carried out at different molt stages by comparing their total hemocyte count(THC) and respiratory burst(RB) and activity of phenol oxidase(PO), nitric oxide synthase(NOS) and lysozyme(LY). Two-way ANOVA showed that salinity and molt stage independently affected THC and RB and the activity of PO, NOS and LY of juvenile L. vannamei significantly(P 0.05). The THC and RB and the activity of NOS gradually increased from the post-molt stages(A and B) to the pre-molt stages(D0–D3), which were common in shrimps inhabiting freshwater and seawater. The activity of PO peaked at the inter-molt stage(C), and touched the lowest at the post-molt stage in freshwater and pre-molt stage in seawater. The activity of LY was stable over the molt cycle. The RB and the activity of PO, NOS and LY of juvenile L. vannamei were significantly lower in freshwater than in seawater; whereas THC was significantly higher in freshwater than in seawater(P 0.05). It was concluded that the post-molt stage(especially stage A) was critical to shrimp culture, which should be intensively attended when L. vannamei was cultured in freshwater.  相似文献   

16.
We tested the utility of chemical marking techniques in the juvenile black rockfish Sebastes schlegelii. Juveniles(30–40 mm total length) were immersed in a range of tetracycline hydrochloride(TC) solutions at concentrations ranging from 300 to 500 mg/L, and alizarin complexone(ALC) solutions at concentrations ranging from 200 to 400 mg/L in filtered sea water(salinity of 30) for 24 h, respectively. Otoliths(sagittae, asteriscus), scales, fin rays(dorsal, pectoral, ventral, anal, and caudal fin rays), and fin spines(dorsal, ventral, and anal fin spines) were sampled and used to detect fl uorescent marks after a 60-day growth experiment. With the exception of 300 mg/L TC, acceptable marks were produced in the otoliths and fin spines by all concentrations of TC and ALC. In particular, we observed clearly visible marks in the sagittae, asteriscus, and fin spines under normal light at concentrations of 200–400 mg/L, 250–400 mg/L, and 250–400 mg/L ALC, respectively. Scales and fin rays had acceptable marks at much higher concentrations(≥350 mg/L TC, ≥250 mg/L ALC for scales and ≥350 mg/L TC, ≥300 mg/L ALC for fin rays). The best mark quality(i.e., acceptable marks were observed in all sampled structures after immersion marking) were obtained following immersion in TC at between 350–500 mg/L, and ALC between 300–400 mg/L. In addition, there was no significant difference in survival and growth of TC and ALC marked fish compared to their controls up to 60 days post-marking( P 0.05).  相似文献   

17.
Accurate assessment of surface suspended sediment concentration(SSSC) in estuary is essential to address several important issues: erosion, water pollution, human health risks, etc. In this study, an empirical cubic retrieval model was developed for the retrieval of SSSC from Yellow River Estuary. Based on sediments and seawater collected from the Yellow River and southeastern Laizhou Bay, SSSC conditions were reproduced in the laboratory at increasing concentrations within a range common to field observations. Continuous spectrum measurements of the various SSSCs ranging from 1 to 5700 mg/l were carried out using an Ava Field-3 spectrometer. The results indicated the good correlation between water SSSC and spectral reflectance(Rrs) was obtained in the spectral range of 726–900 nm. At SSSC greater than 2700 mg/L, the 740–900 nm spectral range was less susceptible to the effects of spectral reflectance saturation and more suitable for retrieval of high sediment concentrations. The best correlations were obtained for the reflectance ratio of 820 nm to 490 nm. Informed by the correlation between Rrs and SSSC, a retrieval model was developed(R2 = 0.992). The novel cubic model, which used the ratio of a near-infrared(NIR) band(740–900 nm) to a visible band(400–600 nm) as factors, provided robust quantification of high SSSC water samples. Two high SSSC centers, with an order of 103 mg/l, were found in the inversion results around the abandoned Diaokou River mouth, the present Yellow River mouth to the abandoned Qingshuigou River mouth. There was little sediment exchange between the two high SSSC centers due to the directions of the residual currents and vertical mixing.  相似文献   

18.
The polychaete Perinereis aibuhitensis, a key species in estuarine ecosystems, can improve the culture condition of sediment. Endosulfan is an organochlorine pesticide used globally to control insects and mites; however, it is a source of pollution in aquaculture as a result of runoff or accidental release. In this study, we evaluated the toxicity of endosulfan to polychaeta and its ability to improve polluted sediment. Specifically, the effects of a series of endosulfan concentrations (0, 1.25, 2.5, 5, 10, 15, and 20 mg/L) were investigated, and the results indicated that the 24-h median lethal concentration (24-h LC50) was 55.57 mg/L, while the 48-h median lethal concentration (48-h LC50) was 15.56 mg/L, and the safe concentration was about 1.556 mg/L. In a 30-d exposure experiment, the animal specimen could decompose endosulfan effectively while improving endosulfan-polluted aquatic sediment.  相似文献   

19.
Nanoparticles,or particles in size of 1-100 nm,are extensively used in the world in different applications.For instance,single-walled carbon nanotubes(SWCNTs) are commonly used in consumer products,such as biosensors,drug and vaccine delivery transporters,and novel biomaterials.Although nanoparticles do not cause safety concerns to consumers who use nanoparticle-containing products,these small particles are potentially harmful for workers who produce them in factories or in cases of discharge to aquatic ecosystems.SWCNTs do not have a natural analogue,so the effects on health of their disposal remain largely unknown.In this study,we evaluated the effects of SWCNTs on a population of the green microalga Chromochloris zofingiensis and the profile and production of pigments and fatty acids.The alga was incubated with SWCNTs for 6 days in 0(control),40,80,160,or 320 mg/L concentrations.SWCNTs showed both positive and negative effects on the growth of C.zofingiensis,with a biomass enhancement at low levels(40-160 mg/L) but inhibition at high levels(320 mg/L).By contrast,a decreased accumulation of fatty acids and pigments of C.zofingiensis was observed over the range of the tested concentrations.These results indicate that the markers on the inhibitive toxicity of SWCNTs are increasingly sensitive in the following order:biomass and fatty acids < primary carotenoids < chlorophylls < secondary carotenoids.C.zofingiensis is a suitable microalga for evaluating the ecotoxicological hazards of SWCNTs,especially in terms of pigmentation response.  相似文献   

20.
【目的】研究波纹龙虾(Panulirus homarus)胚胎对生态因子的耐受能力,为龙虾繁殖提供数据。【方法】采用封闭静水实验方法和常规生物急性毒性实验法。在温度(30±0.5)℃,盐度30,pH(8.10±0.1)和溶解氧(DO)为(5.0±0.5)mg/L条件下,分别进行温度、盐度、pH的骤变和渐变对波纹龙虾胚胎存活影响研究;根据急性毒性预实验结果设置等对数间距浓度值,进行氨氮和亚硝酸盐氮对波纹龙虾胚胎的急性毒性实验。【结果与结论】波纹龙虾胚胎发育的温度、盐度和pH耐受范围分别为7~36℃、15~45和5.8~9.4;波纹龙虾胚胎适宜温度为15~30℃,适宜盐度为30~35,适宜pH为7.0~8.5;氨氮和亚硝酸盐氮毒性效应与浓度和暴露时间呈正相关。氨氮和亚硝酸盐氮暴露24~96 h,波纹龙虾胚胎存活率组间存在显著性差异(P<0.05)。氨氮对波纹龙虾胚胎24、48、72和96 h的半致死浓度(LC50)分别是180.46、61.11、41.87、26.58 mg/L,安全浓度(SC)为2.66 mg/L;亚硝酸盐氮对波纹龙虾胚胎24、48、72和96 h的LC50分别是839.50、423.75、214.48、135.41 mg/L,SC为13.54 mg/L。  相似文献   

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