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We tested the utility of chemical marking techniques in the juvenile black rockfish Sebastes schlegelii. Juveniles(30–40 mm total length) were immersed in a range of tetracycline hydrochloride(TC) solutions at concentrations ranging from 300 to 500 mg/L, and alizarin complexone(ALC) solutions at concentrations ranging from 200 to 400 mg/L in filtered sea water(salinity of 30) for 24 h, respectively. Otoliths(sagittae, asteriscus), scales, fin rays(dorsal, pectoral, ventral, anal, and caudal fin rays), and fin spines(dorsal, ventral, and anal fin spines) were sampled and used to detect fl uorescent marks after a 60-day growth experiment. With the exception of 300 mg/L TC, acceptable marks were produced in the otoliths and fin spines by all concentrations of TC and ALC. In particular, we observed clearly visible marks in the sagittae, asteriscus, and fin spines under normal light at concentrations of 200–400 mg/L, 250–400 mg/L, and 250–400 mg/L ALC, respectively. Scales and fin rays had acceptable marks at much higher concentrations(≥350 mg/L TC, ≥250 mg/L ALC for scales and ≥350 mg/L TC, ≥300 mg/L ALC for fin rays). The best mark quality(i.e., acceptable marks were observed in all sampled structures after immersion marking) were obtained following immersion in TC at between 350–500 mg/L, and ALC between 300–400 mg/L. In addition, there was no significant difference in survival and growth of TC and ALC marked fish compared to their controls up to 60 days post-marking( P 0.05). 相似文献
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Black rockfi sh Sebastes schlegelii juveniles(30–40 mm total length) were immersed in a range of calcein(CAL) solutions at concentrations ranging from 50 to 250 mg/L and alizarin red S(ARS) solutions at concentrations ranging from 100 to 500 mg/L in fi ltered seawater(salinity 30) for 24 h. Fluorescent marks were detected in otoliths(sagittae, asteriscus), scales, fi n rays(dorsal, pectoral, ventral, anal, and caudal fi n rays), and fi n spines(dorsal, ventral, and anal fi n spines) after a 60-d growth experiment. With the exception of 50–100 mg/L CAL, acceptable marks were produced in the otoliths and fi n spines by all concentrations of CAL and ARS. In particular, marks were clearly visible under normal light in the sagittae, asteriscus, and fi n spines of fi sh immersed in 200–500 mg/L, 300–500 mg/L, and 200–500 mg/L ARS, respectively. Scales and fi n rays had acceptable marks at much higher concentrations(≥50 mg/L CAL, ≥300 mg/L ARS for scales and ≥50 mg/L CAL, ≥200 mg/L ARS for fi n rays). The mark quality was highest(i.e., acceptable marks were observed in all sampled structures after immersion marking) in fi sh immersed in 150–250 mg/L CAL or 300–500 mg/L ARS. In addition, there was no signifi cant difference in survival and growth of marked fi sh compared with controls 60 d post-marking( P 0.05). 相似文献
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对许氏平鲉(Sebastes schlegelii)0~90日龄仔、稚、幼鱼的全长、体重进行生物学测定,对其体重、头长、头高、吻长、眼径、体高、肛前长、鳍棘长和鳍条长与全长进行回归分析,确定各指标的异速生长方式、生长拐点出现的时间和早期生长发育特征,结合仔、稚鱼的生态习性,探讨早期生长发育模式和生态学意义。统计学分析结果表明,全长和体重随着日龄的增加其生长规律均符合三次函数关系,全长的生长可分为3个阶段(0~16日龄、17~46日龄、47~90日龄)、体重的生长可分为2个阶段(0~46日龄、47~90日龄),各阶段生长速率有显著差异(P0.05);许氏平鲉早期生长发育过程中,其各量度指标异速生长的拐点均出现在仔鱼向稚鱼(14~24日龄)、稚鱼向幼鱼(46~60日龄)变态的节点之前或附近。研究发现,仔、稚鱼各器官表观形态的改变与变态节点同步。 相似文献
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玛纳斯河流域气候干燥、蒸发强烈,准确估算蒸散发量对地下水资源评价及生态环境保护具有重要指导意义。以往蒸散发研究空间分辨率较低,已不能满足各水文地质分区景观格局演变引起的蒸散发细部变化研究,针对以往不足,文章基于SEBAL模型利用Landsat系列影像估算了近30年来玛纳斯河流域山前平原区蒸散发,并进一步探讨不同水文地质分区蒸散发时空分布特征及影响因素。结果表明,蒸散量空间分布按照水文地质分区呈现明显带状性,各水文地质分区日蒸散总量表现为戈壁带<荒漠带<绿洲带,时间尺度上全区蒸散总量呈上升趋势,且增大幅度逐渐变缓,各分区呈现绿洲带蒸散总量递增、戈壁带及荒漠带蒸散总量先减小后增大,各分区蒸散总量变化趋势是由各分区主地物类型蒸散量变化控制;通过对影响因素的分析可知日蒸散发量随气温的升高而升高,各地物日均蒸散发量与全区平均气温变化趋势一致;归一化植被指数与日蒸散发量在戈壁带与绿洲带呈现较好的正相关关系;地下水位埋深与日蒸散发量在绿洲带呈负相关,当地下水位埋深大于5. 5 m时,日蒸散发量趋于稳定。 相似文献
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The present study compared vertebral deformities of hatchery-reared and wild-caught juvenile Japanese flounder,Paralichthys olivaceus.A total of 362 hatchery-reared flounder(total length 122.5-155.8 mm) were collected from three commercial hatcheries located in Yantai,East China,and 89 wild fish(total length 124.7-161.3 mm) were caught off Yangma Island near Yantai City(37°27′N,121°36′E).All the fish were dissected,photographed,and images of the axial skeleton were examined for vertebral deformities.Compared with wild-caught flounder in which no deformed vertebrae were detected,48(13.3%) hatcheryreared fish had deformed vertebrae.The deformities were classified as compression,compression-ankylosis,and dislocation-ankylosis.The vertebral deformities were mainly localized between post-cranial vertebra 1and 3,with vertebrae number 1 as the most commonly deformed.The causative factors leading to vertebral deformities in reared Japanese flounder may be related to unfavorable temperature conditions,inflammation,damage,or rupture to the intervertebral ligaments under rearing conditions.Furthermore,no significant difference in the total number of vertebral bodies was observed between wild-caught(38.8±0.4) and hatchery-reared flounder(38.l±0.9)(P0.05).However,the number of vertebral bodies of hatchery-reared and wild-caught flounder ranged from 35 to 39 and from 38 to 39,respectively. 相似文献
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