苯并[a]芘(BaP)对褐菖鲉(Sebasticus marmoratus)肝CYP1A1酶活性、基因表达及蛋白表达的影响     

孙文静  王晓艳  祁鹏志  张建设  吴常文 《海洋与湖沼》2018,49(4):897-903

为了了解苯并[a]芘(BaP)对鱼类细胞色素P4501A1(CYP1A1)表达的影响,以褐菖鲉(Sebasticus marmoratus)为实验材料,采用体内实验,研究其在经过不同浓度(0.1、1、10、20、50mg/kg鱼体重量)的BaP诱导后,鱼体肝脏研究CYP1A1基因表达的情况,筛选出后续时间-效应实验中BaP注射的最佳浓度,研究BaP诱导6h、12h、1d、3d、7d后(质量浓度为20mg/kg鱼体重量)鱼体肝脏CYP1A1酶活性、基因表达和蛋白表达的情况。结果表明:剂量-效应实验中,20mg/kg鱼体重量为最佳浓度,此浓度下,基因表达在各组中变化最显著。时间-效应实验中,较空白对照组而言,染毒6h、12h和1d后,EROD酶活性显著增加。3d后开始下降,与对照组相比变化不大,7d后酶活性又发生上调。半定量RT-PCR结果表明,各染毒组与对照组相比,CYP1A1基因表达量都发生了上调,呈现先上升后下降的趋势。其中,6h和12h组相对表达量极显著增加,1d后开始下降且与3d和7d组相比变化不明显。Western blot结果表明,蛋白表达量在染毒12h后表现出显著的诱导效应,随着时间的延长略有回落,但与对照组相比仍有显著性差异。研究表明:BaP对褐菖鲉CYP1A1具有较强的诱导作用。一定质量浓度的BaP注射于褐菖鲉不同的时间后,能诱导褐菖鲉活体EROD酶活性、CYP1A1基因m RNA表达及蛋白表达,并随着时间的延长呈现先诱导后抑制的趋势。这说明BaP作为诱导剂对CYP1A1酶活性和蛋白表达的作用机制可能与调控CYP1A1的转录水平有关。  相似文献   

灰色稳健总体最小二乘估计及高铁路基变形预测     

陈洋  文鸿雁  覃辉  王清涛  周吕 《大地测量与地球动力学》2018,38(2):140-146

最小二乘估计和部分变量误差模型的总体最小二乘估计不具备抵御粗差的能力。鉴于粗差可能同时出现在灰色白化微分方程的观测值和系数矩阵中，本文提出基于IGGⅢ抗差方案的部分变量总体最小二乘稳健估计。结合仿真数据和高铁路基观测数据，系统地比较稳健最小二乘、部分变量总体最小二乘、本文算法参数估计结果和算法稳定性。结果表明，本文算法预测精度高，可以应用到高铁路基沉降预测中。  相似文献   

Suspended sediment and total dissolved solid yield patterns at the headwaters of Urumqi River,northwestern China: a comparison between glacial and non‐glacial catchments          下载免费PDF全文

Wenhua Gao  Shu Gao  Zhongqin Li  X. X. Lu  Mingjun Zhang  Shengjie Wang 《水文研究》2014,28(19):5034-5047

In order to understand the differences in the suspended sediment and total dissolved solid (TDS) yield patterns between the glacial and non‐glacial catchments at the headwaters of Urumqi River, northwestern China, water samples were collected from a glacier catchment and an empty cirque catchment within the region, during three melting seasons from 2006 to 2008. These samples were analyzed to estimate suspended sediment and TDS concentrations, fluxes and erosion rates in the two adjoining catchments. There were remarked differences in suspended sediment and TDS yield patterns between the two catchments. Suspended sediment concentrations were controlled mainly by the sediment source, whereas TDS concentrations were primarily related to the hydrologic interaction with soil minerals. Generally, the glacial catchment had much higher suspended sediment and TDS yields, together with higher denudation rates, than the non‐glacial catchment. Overall, glacial catchment was mainly dominated by physical denudation process, whereas the non‐glacial catchment was jointly influenced by physical and chemical denudation processes. The observed differences in material delivery patterns were mainly controlled by the runoff source and the glacial processes. The melting periods of glacier and snow were typically the most important time for the suspended sediment and TDS yields. Meanwhile, episodic precipitation events could generate disproportionately large yields. Subglacial hydrology dynamics, glaciers pluck and grind processes could affect erodibility, and the large quantities of dust stored on the glacier surface provided additional sources for suspended sediment transport in the glacial catchment. These mechanisms imply that, in response to climate change, the catchment behaviour will be modified significantly in this region, in terms of material flux. Copyright © 2013 John Wiley & Sons, Ltd.  相似文献   

基于TCP-InSAR的采动区铁路动态沉降监测与时序分析     

鹿璐  范洪冬  郑美楠  邹昊 《大地测量与地球动力学》2019,39(2):164-168

为高效获取采动区长时间序列形变，监控煤炭开采对矿区铁路的影响，研究一种基于TCP-InSAR(temporarily coherent point interferometric synthetic aperture radar)的采动区铁路形变监测方法。该方法根据时间序列SAR影像间的相干性，选取临时相干点构建Delaunay三角网，并通过离群值探测去除具有相位模糊度的TCP间的弧段，最后采用最小二乘解算得到区域地表变形。实验使用2016-10~2017-04时间段内的15景Sentinel-1A数据，利用TCP-InSAR技术获得某矿区的铁路形变。结果表明，受采动影响，该时间段内铁路最大下沉值为95 mm，最大倾斜坡度为0.37‰。利用TCP-InSAR技术可实现采动区内铁路长时间的动态形变监测。  相似文献   

基于空间技术实测数据的板块构造运动研究     

孙建军 《测绘与空间地理信息》2016,(3)

将ITRF2008解算结果 GPS、SLR、VLBI实测站速度信息和北美CORS网络、中国陆态网络、欧洲CORS网络站速度信息进行基准统一,联合解算IERS组织划定的全球14个主要大陆板块的欧拉矢量,对原有的板块运动参数进行精化;提出了基于最小二乘法的海量台站选择方法,实验结果表明,该台站选取方法能够有效提高海量台站的筛选效率和可靠性。稳定台站的数量越多,分布越均匀,越能够体现板块运动的现势性。  相似文献   

基于高分一号宽幅多光谱影像的鄱阳湖湿地分类研究     

麻锴  查东平  王宝健 《测绘与空间地理信息》2016,(6):107-110

典型湿地类型分类对于湿地生态环境保护和生态环境建设具有重要意义。本研究以高分一号宽幅多光谱影像数据作为数据源,以鄱阳湖典型湿地作为研究对象,进行典型湿地类型分类。研究采用主成分分析、归一化植被指数、比值植被指数和归一化差异水体指数等方法对不同湿地类型进行光谱特征分析,结果表明:高分一号宽幅多光谱影像反演的归一化植被指数和归一化水体指数能够较好地区分水体、泥滩、挺水植物和湿生植物等常见湿地类型。通过归一化植被指数将湿地类型分为植被和非植被,归一化差异水体指数将植被类型进一步分为挺水植物和湿生植物,将非植被分为水体和泥滩。构建决策树对典型湿地类型进行自动分类,经过精度评价和分析验证,该方法针对高分一号宽幅数据进行典型湿地分类总体精度能够达到82.28%,Kappa系数0.7346,优于常规的监督分类和非监督分类。  相似文献   

高分一号遥感影像地质灾害信息提取方法研究   总被引：1，自引：0，他引：1  

李启源  王明常  王凤艳  谭洋  卢立吉 《测绘与空间地理信息》2016,(2):17-20

山区地质灾害发生频繁,受灾范围大,危害严重。提取地质灾害信息、估算受灾情况,对救灾工作极为重要。传统的遥感人工解译方法速度慢、效率低;计算机自动解译速度快但解译精度受影像质量影响大,大范围地区很难建立起普适性的解译模型。本文利用洮南市西部高分一号遥感数据,结合DEM生成三维影像,建立解译标志,解译基础地质信息,分析地质灾害成因;根据研究区泥石流地质灾害的主要影像特征,提取影像的分类属性,基于面向对象的分类方法,建立信息提取模型,快速提取出地质灾害敏感区域;再进行人机交互,精确提取出地质灾害的类型和范围,通过野外验证,该方法十分可靠,为大范围地质灾害信息快速提取和灾后救援提供科学依据。  相似文献   

L1范数与IQR统计量组合的GNSS坐标序列粗差探测算法     

明锋  曾安敏  景一帆 《测绘科学技术学报》2016,(2):127-132

GNSS坐标时间序列中不可避免地含有粗差,未剔除的粗差将会导致参数估计有偏。因此,粗差探测与剔除是GNSS坐标序列分析中一项重要的数据预处理工作。针对GNSS坐标时间序列特点,提出了一种将L1范数(L1-norm)估计与四分位距统计量IQR(interquartile range)组合的移动开窗粗差探测算法,称之为L1_Mod IQR。该方法的主要思想是,首先利用L1范数估计得到较"真实"的残差,然后再对残差采用IQR统计量进行粗差探测。将L1_Mod IQR法与"3σ"法、基于最小二乘的τ检验法等粗差探测算法进行了模拟计算与对比,验证了该算法的有效性。进一步采用L1_Mod IQR算法对中国区域10个IGS站的高程时间序列进行了分析,结果表明中国区域IGS站高程序列的粗差剔除率最小为0.1%,最大为2.6%。并且以WUHN站为例与SOPAC提供的结果进行了对比,结果表明SOPAC提供的"Clean"数据仍含有大量的粗差,而L1_Mod IQR算法能够有效地剔除粗差。  相似文献   

The Molí del Baró-1 site,a diverse fossil assemblage from the uppermost Maastrichtian of the southern Pyrenees (north-eastern Iberia)     

《Cretaceous Research》2016

The Molí del Baró-1 site (north-eastern Iberia) has yielded one of the richest fossil assemblages of the continental upper Maastrichtian from western Europe. It includes abundant plant (charophytes, sporomorphs, angiosperm leaves, seeds and logs), invertebrate (mollusc shells, partial insect exoskeletons and eggs) and vertebrate (teeth, bones, eggshells) fossils. The fossil assemblage is composed of both parautochthonous and allochthonous remains that mostly deposited in a pool formed at the edge of a meandering river. Plant megafossil evidence suggest that at least three angiosperm species (a willow-like dicot, palms and a likely typhacean monocot) formed the plant community growing in this fluvial environment. Tooth remains indicate that crocodylomorphs were diverse (including atoposaurids, ziphosuchians and eusuchians) which together with small theropod dinosaurs likely consumed prey carcasses or scavenged near the depositional setting. Prismatoolithid eggshells also suggest the presence of nearby nesting area of theropods. On the other hand, hadrosauroids apparently dominated phytophagous faunas including lambeosaurines as well as more basal forms. The taxonomic composition of the Molí del Baró-1 site matches the expected for the timespan and environment and represents a relevant locality to know the diversity of the latest Cretaceous terrestrial ecosystems in south western Europe.  相似文献   

An efficient quasi-Newton approximation-based SORM to estimate the reliability of geotechnical problems     

《Computers and Geotechnics》2016

The first order reliability method (FORM) is efficient, but it has limited accuracy; the second order reliability method (SORM) provides greater accuracy, but with additional computational effort. In this study, a new method which integrates two quasi-Newton approximation algorithms is proposed to efficiently estimate the second order reliability of geotechnical problems with reasonable accuracy. In particular, the Hasofer–Lind–Rackwitz–Fiessler–Broyden–Fletcher–Goldfarb–Shanno (HLRF–BFGS) algorithm is applied to identify the design point on the limit state function (LSF), and consequently to compute the first order reliability index; whereas the Symmetric Rank-one (SR1) algorithm is nested within the HLRF–BFGS algorithm to compute good approximations, yet with a reduced computational effort, of the Hessian matrix required to compute second order reliabilities. Three typical geotechnical problems are employed to demonstrate the ability of the suggested procedure, and advantages of the proposed approach with respect to conventional alternatives are discussed. Results show that the proposed method is able to achieve the accuracy of conventional SORM, but with a reduced computational cost that is equal to the computational cost of HLRF–BFGS-based FORM.  相似文献