长牡蛎碱性磷酸酶的分离纯化及部分性质研究   总被引：5，自引：1，他引：5  

李兴暖  韩雅莉  肖湘  邓瑞鹏  周小朋  管云雁  黄长江 《台湾海峡》2003,22(4):482-486

本文以长牡蛎为酶源提取材料,用正丁醇抽提,硫酸铵分级分离,DEAE-52和Sephadex G-100柱层析,得到一定纯度的碱性磷酸酶,提纯倍数为61.96,比活力达1.326U/mg.该酶的最适pH值9.6,最适温度38℃,初速度6.8μmol/(dm~3·min),米氏常数Km值为1.30mmol/dm~3.实验结果表明,Mg~(2+)对该酶有明显的激活作用;Zn~(2+)、Cu~(2+)、Hg~(2+)对该酶有明显的抑制作用.  相似文献   

Organic matter origin and distribution in suspended particulate materials and surficial sediments from the western Adriatic Sea (Italy)   总被引：5，自引：2，他引：5  

T. Tesi  S. Miserocchi  M.A. Goi  L. Langone  A. Boldrin  M. Turchetto 《Estuarine, Coastal and Shelf Science》2007,73(3-4):431-446

In this study, organic carbon (OC), total nitrogen (TN), stable carbon isotopic (δ¹³C_OC) and CuO reaction product compositions were used to identify the sources of organic matter (OM) and to quantify the relative importance of allochthonous and autochthonous contributions to the western Adriatic Sea, Italy. Suspended particulate material (195 samples) and surficial sediments (0–1 cm, 70 samples) from shallow cross-shelf transects were collected in February and May 2003, respectively. Vertical water column profiles were acquired along the same transects. Data include depth, potential temperature, salinity, density and chlorophyll fluorimetry.Along the western Adriatic shelf in the near-shore region, the phytoplankton growth was influenced by dynamics of the buoyant plumes from the Po and Appennine rivers. A small amount of very fine terrigenous material remained suspended within the coastal current and was exported southward along the shelf to the slope. High variability in the bulk composition was detected in the Po prodelta surficial sediments, whereas the western Adriatic shelf, although a larger area, exhibited a narrower range of values.A significant decoupling was observed between suspended particles in the water column and surficial deposits. The organic material collected in the water column was compositionally heterogeneous, with contributions from marine phytoplankton, riverine–estuarine phytoplankton and soil-derived OM. Frequent physical reworking of surficial sediments likely leads to the efficient oxidation of marine OC, resulting in the observed accumulation and preservation of refractory soil-derived OC delivered by the Po and Appennine rivers.  相似文献   

南海北部及广东沿海新生代火山活动   总被引：17，自引：0，他引：17  

孙嘉诗 《海洋地质与第四纪地质》1991,11(3):45-66,T006

调查区新生代火山活动十分活跃。岩性以基性为主,少数属超基性Sr~(87/86)初始值最高0.706189,最低0.703178,陆区和海盆新生代火山岩均来自地幔。 K-Ar年龄最老62.86百万年,最新0.43百万年。从老至新可划分为8个活动期,分别为古新世早期、始新世早期、渐新世末期、中新世中期、中新世晚期、早更新世早期、早更新世晚期和中更新世中期。 海盆与陆区火山活动在岩性、同位素特征、活动时期、喷发方式以及岩浆来源等方面都十分相似,二者属统一的新生代火山岩区。  相似文献   

碱厂废水光谱特性实验研究     

张绪琴  吴隆业  张世魁  吴曙初  李武 《中国海洋大学学报(自然科学版)》2001,31(4):600-604

该文报道碱厂废水随浓度变化的光谱实验。分析碱厂废水的光谱特征 ,并与相同浓度的海水悬浮泥沙水体的光谱进行比较 ;确定该废水特征波长为 50 1.3nm。根据光谱实验结果 ,结合当前已有的水色传感器的资料 ,选择两个卫星传感器波段 :Sea WIFS第四频道 (497.2～ 52 1.6 nm)和TM第二频道 (518.9～ 6 0 0 .2 nm) ;利用数字对 (R,c) ,建立了反射率与该污染水体浓度间的相关模式。为遥感技术大面积、快速地监测碱厂废水污染水体提供理论依据。  相似文献   

The status of geological dredging techniques     

R. B. Kidd  Q. J. Huggett  A. T. S. Ramsay 《Marine Geophysical Researches》1990,12(1-2):131-143

Scientific sea-floor dredging is currently used in marine geology primarily by the hard-rock community interested in the recovery of basement rock samples from the unsedimented deep ocean floor. The technique has generally been eclipsed by ocean drilling for recovery of sedimentary rocks, because of perceived uncertainties in the location of sampling and in the representativeness of recovered material. This contribution reviews dredging equipment currently in use by marine geological institutions and refers to pinger attachments that allow precise information on the behaviour of the dredge to be telemetered back to the ship. We argue that improvements in ship navigation and transponder navigation at the seafloor, when used in conjunction with surface and/or deeply towed sidescan and swathemapping surveys, now allow for considerably less uncertainty on the location of dredge sampling. Refined sorting criteria for dredge hauls are now also available. Recent comparisons of regional sample recovery by ocean drilling and by dredge sampling indicate that the dredge hauls can usefully supplement the drilling data in the construction of sedimentary and tectonic histories of seafloor areas.  相似文献   

某船坞南翼墙基岩水下控制爆破技术     

徐清明  穆旭 《海岸工程》1997,16(3):47-54

本文介绍的基岩水下爆破属临近重要建筑物的水下控制爆破。当岩石裂隙发育，且岩面有较厚的石碴覆盖层，其技术难度大，采取的诸如搭钢平台钻孔。塑料套管护孔，以及孔间、排间塑料导爆管雷管接力式微差起爆网路等措施，都是成功之经验，值得今后类似工程推广应用。  相似文献   

黄海海域陆相中生界油气远景   总被引：8，自引：1，他引：8  

李刚  陈建文  肖国林  温珍河  戴春山  龚建明  闫桂京 《海洋地质前沿》2003,19(8):7-11

黄海海域油气勘探已40余载，尚未实现油气突破，其找油前景引起石油地质学家的极大关注。根据前人的认识和最新研究成果，结合邻区油气地化资料，探讨了黄海海域中生界地层生、储、盖特征，特别是白垩系，它分布广，厚度大，烃源岩达到较好生油岩标准，并且生、储、盖组合理想，中生界是该区下部油气勘探首选目标。  相似文献   

产复合酶菌株Pseudomonas sp.NJ197产酶发酵条件的研究   总被引：2，自引：0，他引：2  

张金伟  曾润颖 《台湾海峡》2005,24(4):520-525

从南极普利兹湾深海900m的沉积物中筛选到一株同时产多种低温复合酶的菌 株NJ197,作者对其进行碳源、氮源、无机盐、起始pH值、培养时间、接种量等发酵条 件的优化实验．实验结果证明,在以0．5％可溶性淀粉为碳源,以0．5％豆饼粉为氮 源,无机盐:0．424％NH4H2PO4、0．075％K2HPO4、0．02％MgSO4、0．5％CaCO3,起始 pH值8．5,接种36h种龄的种子10％,20℃、250r／min的条件下在旋转摇床中培养 72h,产复合酶菌株产脂肪酶和淀粉酶活性最高．最佳的生理条件下复合酶中的碱性 脂肪酶和淀粉酶的产量分别提高到22．4U／cm3和30．9U／cm3,该产复合酶菌株可以 作为诱变育种、基因工程菌改造的出发菌株,在洗涤和制革工业中有良好的应用前 景．  相似文献   

氯化物型盐碱地池塘底泥中水溶性盐的分布特征     

张美昭  张兆琪  郑伟刚  董双林 《海洋湖沼通报》2001,(4):50-55

1997－1999年夏天，对位于黄河下游的氯化物型盐碱地池塘底泥中的水溶性盐进行了分析。结果表明：底泥中的含盐量，SO^2-4,Ca^2 ,Mg^2 含量是随着塘龄的增加而升高，而其余离子基本不随塘龄的增加而变化。各离子的垂直分布为“泥－土”混合层的含量较低，“水－泥”交界层含量较高。  相似文献   

思林水电站通航建筑物高边坡稳定分析和评价     

徐力群  沈振中  俞凯加  吕军 《岩土力学》2008,29(Z1):42-46

应用ANSYS结构分析软件,模拟了思林水电站通航建筑物高边坡的地形、地质条件以及开挖的过程,建立边坡开挖的有限元计算分析模型,分析边坡在自然状态、分级开挖以及锚杆支护加固等工况下的应力、变形及其整体稳定性.定义单元的局部安全系数,得到了边坡开挖加固后稳定安全系数的分布.根据计算成果,指出现设计加固措施可以保证边坡稳定,无需增加其他工程措施.  相似文献