Analysis of cultivable aerobic bacterial community composition and screening for facultative sulfate-reducing bacteria in marine corrosive steel     

LI Xiaohong  XIAO Hui  ZHANG Wenjun  LI Yongqian  TANG Xuexi  DUAN Jizhou  YANG Zhibo  WANG Jing  GUAN Fang  DING Guoqing 《海洋湖沼学报（英文）》2019,(2):600-614

Anaerobic, aerobic, and facultative bacteria are all present in corrosive environments. However, as previous studies to address corrosion in the marine environment have largely focused on anaerobic bacteria, limited attention has been paid to the composition and function of aerobic and facultative bacteria in this process. For analysis in this study, ten samples were collected from rust layers on steel plates that had been immersed in seawater for diff erent periods (i.e., six months and eight years) at Sanya and Xiamen, China. The cultivable aerobic bacterial community structure as well as the number of sulfate-reducing bacteria (SRB) were analyzed in both cases, while the proportion of facultative SRB among the isolated aerobic bacteria in each sample was also evaluated using a novel approach. Bacterial abundance results show that the proportions are related to sea location and immersion time;abundances of culturable aerobic bacteria (CAB) and SRB from Sanya were greater in most corrosion samples than those from Xiamen, and abundances of both bacterial groups were greater in samples immersed for six months than for eight years. A total of 213 isolates were obtained from all samples in terms of CAB community composition, and a phylogenetic analysis revealed that the taxa comprised four phyla and 31 genera. Bacterial species composition is related to marine location;the results show that Firmicutes and Proteobacteria were the dominant phyla, accounting for 98.13% of the total, while Bacillus and Vibrio were the dominant genera, accounting for 53.06% of the total. An additional sixfacultative SRB strains were also screened from the isolates obtained and were found to encompass the genus Vibrio (four strains), Staphylococcus (one strain), and Photobacterium (one strain). It is noteworthy that mentions of Photobacterium species have so far been absent from the literature, both in terms of its membership of the SRB group and its relationship to corrosion.  相似文献   

高通量测序技术揭示印度尼西亚北苏拉威西岛一株色素异常澄黄滨珊瑚特异的共生微生物群落     

欧丹云  陈彬  HADI Tri Aryono  SUHARSONO null  牛文涛  ALFIANSAH Yustian Rovi 《海洋学报(英文版)》2018,37(12):78-84

细菌性疾病对珊瑚的健康影响造成了很大影响,而致病菌与珊瑚疾病之间的对应性还不明朗。色素异常现象是野外澄黄滨珊瑚一种常见的不健康状态。我们采用Illumina 16s rRNA基因高通量测序技术比较分析了健康和色素异常澄黄滨珊瑚共生细菌的群落组成差异。色素异常澄黄滨珊瑚共生细菌的多样性明显高于健康澄黄滨珊瑚,同时两种珊瑚的共生细菌群落组成也有很大差异。一些特定的细菌类群在澄黄滨珊瑚共生细菌中的比例很高,推测这些细菌种类可能是导致澄黄滨珊瑚色素异常的“罪魁祸首”。  相似文献   

The bacterial diversity in surface sediment from the South China Sea   总被引：1，自引：0，他引：1  

WANG Guanghu  DONG Junde  LI Xiang  SUN Huimin 《海洋学报(英文版)》2010,29(4):98-105

16S rDNA sequencing results from this study and literatures demonstrate that sediment bacteria in the South China Sea (SCS) were very diverse,which contained 22 of the 24 phyla of bacteria investigated from marine sediment,however,it was very imbalance among stations.So bacterial diversity from 15 samples which covered a wide range of sediment types from 20 to 3 888 m in depth was studied in DGGE (denature gradient gel electrophoresis) in this paper.The DGGE results indicate that both sediment bacterial diversity and diversity difference among stations were significant.Thirty representative and differential fingerprints among samples were recovered and sequenced,phylogenetic analysis indicates that they may belong to Proteobacteria (α-,β-,γ-,δ-,ε-),Planctomycetes,Firmicutes,Chloroflexi,Acidobacteria,Actinobacteria,Nitrospirae,Gemmatimonadetes,candidate division WS3 and so on,of which,Gemmatimonadetes and candidate division WS3 bacteria were first detected in SCS sediment.This study also shows that bacterial diversity analysis based on DGGE was more potential than traditional 16S rDNA clone library in multiple sample analysis.  相似文献   

新疆博斯腾湖浮游细菌丰度对富营养化及咸化的响应   总被引：4，自引：2，他引：2  

赛&#;巴雅尔图  黄瑾  谢贵娟  冯丽  胡随喜  汤祥明 《湖泊科学》2011,23(6):934-941

本文以地处干旱地区我国最大的内陆淡水湖——博斯腾湖为研究对象,于2010年6月,在博斯腾湖大小湖区共选取23个采样点,采用表面荧光显微镜直接计数法( FDC)研究了水体中浮游细菌丰度的空间分布规律及其与环境因子的关系.结果表明:博斯腾湖水体中总氮(TN)浓度在0.26-1.45 mg/L之间变动(均值0.91 mg/L...  相似文献   

Effect of Biochar and Hyper-Thermal Inoculum Addition on Polycyclic Aromatic Hydrocarbon-Degrading Bacteria during Composting     

Qingjun Zhang  Tong Zhu  Youzhao Wang  Sai Yao  Ning An  Qingxiang Xiao 《洁净——土壤、空气、水》2023,51(4):2200114

The effects of biochar and hyper-thermal inoculum on polycyclic aromatic hydrocarbon-degrading bacterial communities (PAHBC) during the livestock manure composting are studied here. The experiment is performed on composting of livestock manure and wheat straw amended with biochar and hyper-thermal inoculum. Physicochemical properties, enzyme activity, polycyclic aromatic hydrocarbons (PAHs), and microbial activities are monitored and the comprehensive assessment is analyzed during the composting process. The results show that the dominant phyla Firmicutes, Proteobacteria, and Actinomycetes that are potential PAH-degrading bacterial community hosts are enriched. Meanwhile, the addition of biochar and hyper-thermal inoculum enhances the abundance of PAHBC. Therefore, the biostimulation of biochar and hyper-thermal inoculum could affect the frequency and composition of PAHBC.  相似文献   

一株耐冷异养硝化菌的分离鉴定及脱氮特性   总被引：1，自引：0，他引：1  

周英萍  唐美珍  曹彦雪  周高洁  陈娟娟  马文秀 《湖泊科学》2019,31(3):746-754

利用异养硝化培养基,冬季从南四湖人工湿地底泥中分离筛选到一株具有较高脱氮性能的菌株Z01.经形态特征、生理生化特性以及16S rDNA序列分析,初步鉴定该菌株为假单胞菌属（Pseudomonas sp.）,命名为Pseudomonas sp.Z01.实验探究得到菌株Z01的最佳初始投菌量为7%,最适温度、pH和盐度分别为15℃、6.0~8.0和0.3%,最佳碳源为葡萄糖.在最适生长条件下,通过氨氮去除试验发现该菌株对模拟污水中氨氮的去除率有一定提高,是异养硝化培养基中氨氮去除效率的1.1倍.此外,该菌株对模拟废水中总氮和COD也具有较好的去除效果.研究表明,菌株Z01在冬季污水处理中硝化脱氮具有良好的应用前景和很大的发展空间.  相似文献   

海洋沉积物中硝化和反硝化过程研究进展   总被引：3，自引：0，他引：3  

曹文卿  刘素美 《海洋科学进展》2006,24(2):259-265

硝化和反硝化过程是氮循环的中心环节。水生态环境中氮的转化受诸多因素的制约,了解这些控制要素的作用机理,对预防和消除水体富营养化或缓解初级生产的氮限制有重要意义。综述了硝化和反硝化的研究现状,着重介绍了硝化-反硝化影响因素及测量方法的研究进展,并预测了进一步研究重点。  相似文献   

反硝化条件下河岸渗滤过程中苯胺的降解   总被引：2，自引：0，他引：2  

吴耀国  曾睿  惠林  尹德忠 《地球科学》2006,31(2):273-278

通过河岸渗滤作用(riverbankfiltration, RBF) 诱发河水的补给, 增大地下水的允许开采量可以满足更多居民生活饮用水需求.受人类活动的影响, 河流等地表水体遭受苯胺污染, 可能通过RBF进入地下水, 以致饮用该地下水存在健康风险.为研究反硝化条件下, 苯胺在RBF中的转化, 采集渭河河床沉积物及沿岸地下水含水层的含水介质, 装置土柱, 进行土柱动态模拟实验.经过153 d的实验研究发现, 利用苯胺对RBF中土著反硝化微生物进行驯化, 大约经过37d菌种完全适应.具有该菌种的RBF系统, 对苯胺具有巨大降解能力, 在NO3——N约为23.0 mg/L的条件下, RBF系统可使40、80甚至400 mg/L浓度的苯胺100%降解, 矿化率分别达97.99%、91.39%与75.30%.反硝化条件下, 苯胺在RBF中的降解仅有少部分经过脱氨作用, 绝大部分与腐殖质以共价键形式形成耦合物, 该耦合物更易为微生物降解, 且降解过程中不产生对研究环境微生物有毒的中间产物, 可实现反硝化条件下RBF中苯胺的连续降解.   相似文献   

一种消除九孔鲍苗细菌性病原的无公害绿色生物方法的研究   总被引：8，自引：0，他引：8  

宋志萍  蔡俊鹏  王志  韩韫 《海洋科学》2006,30(1):44-48

从海洋环境中分离出4株蛭弧菌(Bdellovibriosp.),并对34株九孔鲍(Haliotisdiversicolor)苗细菌性病原进行了裂解试验。结果表明,Bh04-4、Bh04-41a、Bh04-A 和Bh04-1f等4株蛭弧菌分别可裂解11株、13株、22株和28株病原菌,裂解率为32.4%、38.2%、64.7%和87.5%;4株共同作用,则可裂解32株病原菌,裂解率高达94.1%。研究结果展示了应用蛭弧菌控制九孔鲍苗细菌性病原的可行性。  相似文献   

Greigite (Fe3S4) as an indicator of drought – The 1912–1994 sediment magnetic record from White Rock Lake, Dallas, Texas, USA     

Richard L. Reynolds  Joseph G. Rosenbaum  Peter van Metre  Michele Tuttle  Edward Callender  Alan Goldin 《Journal of Paleolimnology》1999,21(2):193-206

Combined magnetic and geochemical studies were conducted on sediments from White Rock Lake, a reservoir in suburban Dallas (USA), to investigate how land use has affected sediment and water quality since the reservoir was filled in 1912. The chronology of a 167-cm-long core is constrained by the recognition of the pre-reservoir surface and by 137Cs results. In the reservoir sediments, magnetic susceptibility (MS) and isothermal remanent magnetization (IRM) are largely carried by detrital titanomagnetite that originally formed in igneous rocks. Titanomagnetite and associated hematite are the dominant iron oxides in a sample from the surficial deposit in the watershed but are absent in the underlying Austin Chalk. Therefore, these minerals were transported by wind into the watershed. After about 1960, systematic decreases in Ti, Fe, and Al suggest diminished input of detrital Fe-Ti oxides from the surficial deposits. MS and IRM remain constant over this interval, however, implying compensation by an increase in strongly magnetic material derived from human activity. Anthropogenic magnetite in rust and ferrite spherules (from fly ash?) are more common in sediment deposited after about 1970 than before and may account for the constant magnetization despite the implied decrease in detrital Fe-Ti oxides. An unexpected finding is the presence of authigenic greigite (Fe3S4), the abundance of which is at least partly controlled by climate. Greigite is common in sediments that predate about 1975, with zones of concentration indicated by relatively high IRM/MS. High greigite contents in sediment deposited during the early to mid-1950s and during the mid-1930s correspond to several-year periods of below-average precipitation and drought from historical records. Relatively long water-residence times in the reservoir during these periods may have led to elevated levels of sulfate available for bacterial sulfate reduction. The sulfate was probably derived via the oxidation of pyrite that is common in the underlying Austin Chalk. These results provide a basis for the paleoenvironmental interpretation of greigite occurrence in older lake sediments. The results also indicate that greigite formed rapidly and imply that it can be preserved in the amounts produced over a short time span (in this lake, only a few years). This finding thus suggests that, in some lacustrine settings, greigite is capable of recording paleomagnetic secular variation.  相似文献