鹧鸪菜组织和细胞培养的研究Ⅳ.藻体任意碎片的培养与损伤藻体再生的观察     

王志勇 《海洋湖沼通报》1991,(1)

把鹧鸪菜藻体切成大小不同的切段、以及任意切碎,培养于添加氮、磷素的消毒海水中,后者分10℃、15℃、20℃、25℃四种不同温度条件培养,结果全部切段、切碎片都很快再生出假根附着于基质,并再生出一至多个叶状体;叶状体长大后从基部长出新的假根成为完整的幼苗(再生苗)。试验表明,大的切段其再生苗生长快、长度大,但出苗率(一定长度的藻体长出再生苗数量)低。培养温度较高时(20—25℃),藻体任意切碎片再生速度快、附着速度快、数量多且较牢固。利用藻体切碎片来培养鹧鸪菜幼苗是可行的;培养的适宜温度为20℃以上、海水比重为1.010左右。本文还对鹧鸪菜损伤藻体再生的情况(繁殖生殖学)进行一些观察和描述。  相似文献   

海上平台桶形基础模型压力压贯与负压沉贯试验研究   总被引：1，自引：0，他引：1  

朱儒弟  何生厚 《海岸工程》1999,18(1):60-66

介绍了海上平台桶形基础模型（模型桶）压力压贯和负压沉贯的试验概况,对比分析了两者贯入力的巨大差异和产生的原因,给出了模型桶压力压贯中贯入深度与压力的关系,负压没贯负压与贯入深度,抽吸泵流量,基土渗流量,桶内土塞隆起之间的关系,试验结果表明,负压沉贯可以大大降低以砂质粉土为基土的土抗力,为在胜利油田类似基土海域海上平台应用桶形基础提供了试验依据,为海上现场导管架桶形基础平台的安装就位施工和控制提供了  相似文献   

中华半管藻繁殖生物学研究──Ⅱ.营养细胞和休眼孢子的形态结构     

郑磊  林均民  金德祥 《台湾海峡》1994,(3)

通过扫描电镜观察中华半管藻的营养细胞和休眠孢子的形态结构显示：营养细胞上下壳各具一个线性裂口而不外突的唇形突,壳壁上的眠纹放射状,爪状连接刺上有零散的单孔,从单孔分泌胶质与相邻细胞连接;休眠孢子双凸透镜形或近球形,壳厚,表面平滑无纹饰,上壳面边缘具8～16根分叉长刺,下壳面具微刺,外围有一裙状物,其上有微孔。两类细胞不同的形态特征反映其不同的生态适应。  相似文献   

不同软基处理方法适用性比较   总被引：8，自引：0，他引：8  

熊丛博  于定勇 《海岸工程》2003,22(1):20-26

总结分析了国内外现有的软土地基处理的各种方法。通过对采用不同方法进行软基处理的工程实例处理效果的定量对比，发现不同软基处理方法的适用性差异很大，多数工程中仅用一种方法很难达到设计要求，而两种或多种方法联合则可望取得更好的效果。  相似文献   

桶形基础平台三维有限元稳定性分析     

钱荣  周锡礽  孙克俐  张伟 《海洋技术学报》2003,22(4):49-53

以我国第一座桶形基础采油平台工程实例为原型，采用三维有限元计算方法对桶形基础稳定性分析。平台在波浪、风等荷载作用下，其稳定性不仅与平台的整体结构有关，而且与桶形基础及其周围土体相互作用相关。在计算中，利用薄层摩擦单元模拟土与结构间的相互作用，取得了很好的效果。分析结果表明桶形基础采油平台在设计条件下运行是较为稳定的。  相似文献   

Recruitment patterns of Serpula vermicularis L. (Polychaeta, Serpulidae) in Loch Creran, Scotland   总被引：1，自引：0，他引：1  

Nicola D. Chapman  Colin G. Moore  Dan B. Harries  Alastair R. Lyndon 《Estuarine, Coastal and Shelf Science》2007,73(3-4):598-606

This study aimed to contribute to conservation management of reefs of Serpula vermicularis by increasing understanding of the factors influencing larval settlement. The study was carried out in Loch Creran, which supports the most extensive known development of S. vermicularis reefs in the world. Settlement plates were deployed to examine the influence of season, depth, reef density, substrate type and orientation. Monthly deployment of plates revealed settlement of S. vermicularis to occur predominantly from mid-June to mid-October, peaking in late August to early September. Settlement of Pomatoceros spp. peaked much earlier, in late May to early June. Deployment of plates at different depths revealed a marked reduction in S. vermicularis settlement intensity between 6 and 12 m. As this corresponds with the deeper limit of the peripheral fringe of serpulid reefs in the loch, it is suggested that this limit is imposed by a depth-correlated settlement response, rather than reduction in available substrata. Comparisons of various substrata showed a preference by S. vermicularis larvae for a slate over a scallop substrate and no evidence of enhanced recruitment to occupied or unoccupied tubes of S. vermicularis, suggesting that gregarious attraction is unlikely to be a factor causing reef formation. Settlement onto the upper side of a horizontal scallop substrate was found to be insignificant in comparison with the underside or a vertically orientated scallop. Evidence for the role of light in controlling the depth and substrate-orientation preferences of S. vermicularis larvae is discussed. Based on the results of this study, recommendations are made regarding remediation of areas suffering reef damage.  相似文献   

Particulate peptidoglycan in seawater determined by the silkworm larvae plasma (SLP) assay     

Akira Shibata  Kouichi Ohwada  Masakazu Tsuchiya  Kazuhiro Kogure 《Journal of Oceanography》2006,62(1):91-97

Peptidoglycan (PG) is a biopolymer found exclusively in the cell wall of bacteria. Recent chemical analysis of particulate organic matter suggests that a major amount of the muramic acid, an amino sugar present only in PG, could not be accounted for in terms of bacterial cells (Benner and Kaiser, 2003); however, data on particulate PG is quite sparse. In the present study, conducted in 1996, the PG was examined at 5 sampling sites in the northwestern Pacific Ocean, and in natural seawater cultures. Particulate PG, which was concentrated using a 96-well filtration plate equipped with Durapore filters (pore size, 0.22 μm), was measured by the silkworm larvae plasma (SLP) assay. The PG concentration generally decreased with depth and correlated significantly with bacterial abundance throughout the entire water column. However, the ratio of particulate PG to bacterial abundance varied with depth. The average ratio was 0.61 ± 0.53 (average ± SD, n = 40) between 50 and 2000 m, which agreed with the bacterial cellular PG content from 0.63 to 1.1 fg cell⁻¹ obtained in seawater cultures. On the other hand, the ratios of PG to bacteria from the surface to 50 m (3.7 ± 2.6, n = 29) and below 2,000 m (2.1 ± 1.7, n = 7) were significantly higher than that between 50 and 2,000 m. These results may suggest that, in the surface and deep layers, a significant fraction of particulate PG was present in bacterial detritus, whereas this fraction was reduced in the middle layer.  相似文献   

Seasonal population dynamics of Octopus vulgaris in the eastern Mediterranean     

Katsanevakis  Stelios; Verriopoulos  George 《ICES Journal of Marine Science》2006,63(1):151-160

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向阳圩海塘围堰堤坝沉降特性原位实测及稳定性分析     

张敏娟戴旻  黄醒春 《海洋工程》2005,23(2):92-97

以上海市向阳圩海塘围堰主堤为工程背景，在大量原位实测的基础上通过对各测试结果的统计分析研究了软土海滩围堰堤坝及地基沉降位移特性。结果表明：围堰堤坝及地基沉降具有显著的时效特性，沉降与时间的相关关系可用负指数时效特性曲线拟合；各测点位移值随时间具有明显突变变化，整个沉降时域可分为快速沉降时域区及缓慢沉降时域区。同时，在充分考察了沉降速率随时间变化特性的前提下提出了用该参量预测堤坝稳定时间及最终沉降位移的建议。  相似文献   

新建水坝对下游已建水工建筑物的影响分析     

宋子东 《海岸工程》2005,24(1):59-62

分析了新建水坝及社会经济发展因素对坝下游水流及泥沙的影响，用一个简易河床模型来说明新建大坝后下游河床的演变规律，通过坝下游河床一般演变分析来阐述局部冲刷对已建水工建筑物基础的影响及对已建水工建筑基础的防护措施。  相似文献