一株海洋细菌MZ0306A1的16SrDNA扩增及序列分析     

闵怀  黄备  刘小宇  许强芝  唐静亮  胡颢琰  王婕妤 《海洋科学》2008,32(10)

PCR技术应用于环境监测具有快速、灵敏、准确、简便、特异性强等特点,被广泛应用于水体、气体和土壤的环境污染监测中。将此方法应用于海洋细菌监测中,从浙江南部沿海表层海水中分离到一株海洋细菌MZ0306A1,利用细菌通用引物对其16SrDNA进行扩增。琼脂糖凝胶电泳检测目的DNA片段约为1．5kb。测序后将所测得的序列在NCBI网站中进行相似性搜索,得到该细菌的结果与Halomonas variabilis strain DSM 3051的相似性最高,为97％,故命名为嗜盐单胞菌属。该细菌的16SrDNA序列已经提交GenBank,序列收录号为EU124745。  相似文献   

海洋石油烃降解细菌T₁和T₂的分子鉴定分类     

田胜艳  王娟  聂利红 《海洋科学》2008,32(9):25-28

从天津滨海潮间带被石油烃严重污染的沉积物(千样含油量0.2g/g)中, 筛选分离出能够以柴油为唯一碳源生长的细菌, 对其中生物量大、单株菌降解效率较高的两株细菌T₁和T₂进行16S rDNA克隆, 通过测定和比较16S rDNA的部分序列对这两株细菌进行分子鉴定, 以期用于石油污染的微生物修复中。结果表明,T₁与深红红螺菌(Rhodospirillum rubrum)的同源性为89%,T₂与施氏甲单抱杆菌  相似文献   

The bacterial diversity in surface sediment from the South China Sea   总被引：1，自引：0，他引：1  

WANG Guanghu  DONG Junde  LI Xiang  SUN Huimin 《海洋学报(英文版)》2010,29(4):98-105

16S rDNA sequencing results from this study and literatures demonstrate that sediment bacteria in the South China Sea (SCS) were very diverse,which contained 22 of the 24 phyla of bacteria investigated from marine sediment,however,it was very imbalance among stations.So bacterial diversity from 15 samples which covered a wide range of sediment types from 20 to 3 888 m in depth was studied in DGGE (denature gradient gel electrophoresis) in this paper.The DGGE results indicate that both sediment bacterial diversity and diversity difference among stations were significant.Thirty representative and differential fingerprints among samples were recovered and sequenced,phylogenetic analysis indicates that they may belong to Proteobacteria (α-,β-,γ-,δ-,ε-),Planctomycetes,Firmicutes,Chloroflexi,Acidobacteria,Actinobacteria,Nitrospirae,Gemmatimonadetes,candidate division WS3 and so on,of which,Gemmatimonadetes and candidate division WS3 bacteria were first detected in SCS sediment.This study also shows that bacterial diversity analysis based on DGGE was more potential than traditional 16S rDNA clone library in multiple sample analysis.  相似文献   

Phylogenetic analysis of aerobic anoxygenic phototrophic bacteria and their relatives based on farnesyl pyrophosphate synthase gene     

FENG Fuying  JIAO Nianzhi  DU Hailian  ZENG Yonghui 《海洋学报(英文版)》2010,29(5):82-89

The study aims to reveal phylogenetic and evolutionary relationship between aerobic anoxygenic phototrophic bacteria (AAnPB) and their relatives, anaerobic anoxygenic phototrophic bacteria (AnAnPB) and nonphototrophic bacteria (NPB, which had high homology of 16S rDNA gene with AAnPB and fell into the same genus), and validate reliability and usefulness of farnesyl pyrophosphate synthase (FPPS) gene for the phylogenetic determination. FPPS genes with our modified primers and 16S rDNA genes with general primers, were amplified and sequenced or retrieved from GenBank database. In contrast to 16S rDNA gene phylogenetic tree, AAnPB were grouped into two clusters and one branch alone with no intermingling with NPB and AnAnPB in the tree constructed on FPPS. One branch of AAnPB, in both trees, was located closer to outgroup species than AnAnPB, which implicated that some AAnPB would be diverged earlier in FPPS evolutionary history than AnAnPB and NPB. Some AAnPB and NPB were closer located in both trees and this suggested that they were the closer relatives than AnAnPB. Combination codon usage in FPPS with phylogenetic analysis, the results indicates that FPPS gene and 16S rRNA gene have similar evolutionary pattern but the former seems to be more reliable and useful in determining the phylogenic and evolutionary relationship between AAnPB and their relatives. This is the first attempt to use a molecular marker beside 16S rRNA gene for studying the phylogeny of AAnPB, and the study may also be helpful in understanding the evolutionary relationship among phototrophic microbes and the trends of photosynthetic genes transfer.  相似文献   

污水人工快速渗滤系统中氨氧化菌16S rDNA的DGGE分析     

马鸣超  姜昕  刘菲  李俊 《高校地质学报》2007,13(4):688-693

为了研究污水人工快速渗滤系统(CRI)中氨氧化菌的空间分布规律以及NH4-N在CRI中的迁移转化机理,从深圳运行中的CRI系统砂层填料中以5~10cm间隔垂直取12个样品,对其氨氧化菌的16SrDNA进行DGGE分析。结果表明,CRI系统中氨氧化菌菌群随着深度的增加先增多后减少,在表层至10cm处氨氧化菌约有4~5种组成,主要由某些以有机氮为降解底物的微生物和进水中被砂粒介质截流吸附的微生物组成;在20~90cm范围内氨氧化菌增加至9种左右,多样性最为丰富,是亚硝化作用发生的主要区域;而在100cm以下的范围,由于NH4-N浓度的降低或系统复氧不足,氨氧化菌迅速减少至5~6种。该研究结果证明了氨氧化菌在快渗池内分布范围的变化对NH4-N去除率的影响,即氨氧化菌在快渗池内分布的越接近中上层,对NH4-N的去除效果越好。关于CRI系统中氨氧化菌的空间分布规律及其与NH4-N去除率关系的认识,为工艺改进和效果提高提供了理论基础。  相似文献   

南海细菌胞外多聚物的神经保护活性筛选          下载免费PDF全文

卫茂生  王清池  耿丽华  岳洋  孙超岷  张全斌 《海洋科学》2023,47(3):79-88

本文采取传统的醇沉水溶的工艺提取了深海细菌的胞外聚合物(extracellularpolymericsubstance,EPS),使用过氧化氢作为损伤剂建立SH-SY5Y人神经母细胞瘤体外氧化损伤模型。在安全剂量下评价EPS的体外神经保护活性,确定16种活性良好(细胞活力>90%)的EPS。16种EPS的成分分析结果如下:1)16种EPS的分子量分布呈三段式,分别为>200 kDa, 10～200 kDa和<10 kDa; 2)16种EPS单糖组成中除甘露糖、葡萄糖外,还有稀有单糖氨基葡萄糖、鼠李糖、葡萄糖醛酸、氨基半乳糖、半乳糖、岩藻糖; 3)EPS的总糖含量为14.06%～21.40%,蛋白含量为10.66%～31.05%,硫酸基含量为2.91%～26.53%。通过对相应菌株进行16SrDNA全序列分析并与GenBank比对,完成了活性菌株的鉴定,通过MEGA7.0和Figtree构建系统进化树。鉴定结果表明,16株深海细菌归属于3门8科9属16种,分布在三个门类:厚壁菌门(Firmicutes, 9/16),变形菌门(Proteobacteria, 6/16)和...  相似文献