Development of a real-time PCR method for Thalassiosira rotula rapid detection     

HE Shanying  YU Zhigang  MI Tiezhu 《海洋学报(英文版)》2007,26(2):133-139

Gene specific primers and DNA probe were designed based on the sequence of 18S rDNA cloned from the red tide alga Thalassiosira rotula. A real-time fluorescent quantitative PCR (RFQ-PCR) method was developed for quantitative detection of T.rotula. The RFQ-PCR assay data showed that the results obtained with the RFQ-PCR quite good agreement with those with the light microscope (LM) counting method, which suggested that the RFQ-PCR could be a useful method for red tide alga detection.  相似文献   

Estimation of Uplift Capacity of Rapidly Loaded Plate Anchors in Soft Clay     

S. P. Singh  D. P. Tripathy  S. V. Ramaswamy 《Marine Georesources & Geotechnology》2007,25(3):237-249

Plate anchors are extensively used in civil engineering constructions as they provide an economical alternative to gravity and other embedded anchors. The rate of loading is one of the important factors that affects the magnitude of soil resistance as well as soil suction force. This article outlines the effect of pullout rate on uplift behavior of plate anchors (70 mm diameter) buried in soft saturated clay by varying the pullout rate from 1.4 mm/min to 21.0 mm/min. The variation of breakout force and suction force with embedment depth and rate of pull are presented. A correlation between the rate of increase of undrained strength of clay and anchor capacity with rate of strain has been established. Finally an empirical equation has been proposed that includes the rate of pull in the estimation of breakout capacity of anchors.  相似文献   

真鲷早期发育阶段行为生态学研究     

刘晓春  何大仁  李大勇 《热带海洋学报》1993,(3)

真鲷Pagrosomus major Temminck et Schlegel早期发育阶段游泳行为的发育可以分为:(1)旋转式运动;(2)阶梯型运动;(3)间断式水平运动和滑翔;(4)巡游以及(5)疾游五种类型。前期仔鱼的摄食方式主要是伴有甩头动作的前冲摄食;后期仔鱼的摄食主要有两种类型:一种是身体弯成S形的“S攻击型”;另一种为不作摄食姿势的“咬食攻击型”。摄食方式随着发育水平和饵料种类的不同而异。文中对发育过程中出现的攻击行为、护食行为、互食行为、猝死现象亦进行了描述。并对游泳、摄食以及各种行为的生态意义和实际意义进行了讨论。  相似文献   

Ex situ target strength of rockfish (Sebastes schlegeli) and red sea bream (Pagrus major) in the Northwest Pacific   总被引：2，自引：0，他引：2  

Kang  Donhyug; Hwang  Doojin 《ICES Journal of Marine Science》2003,60(3):538-543

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应用遗传神经网格方法分析赤潮监测数据   总被引：1，自引：1，他引：1  

杨建强  高振会  孙培艳  石强  李钦亮 《海洋科学进展》2002,20(2):77-82

简要介绍了遗传神经网络方法的原理 ,探讨了应用遗传神经网络方法研究辽东湾海域丹麦细柱藻 (Leptocylindrusdanicus)赤潮与其环境因子之间的关系 ,计算了各环境因子对丹麦细柱藻赤潮的贡献。结果表明 ,温度、盐度、DIN的变化对研究海域丹麦细柱藻种群密度的增长有比较重要的影响 ,DIN是营养限制因子。遗传神经网络是分析赤潮监测数据的有效方法 ,值得进一步探索  相似文献   

浙江南部海域富营养化和赤潮的探讨   总被引：6，自引：2，他引：6  

蒋国昌 《海洋学研究》1993,11(2):55-61

本文探讨了浙江南部海域1990年春季发生赤潮期间的水化学特征和富营养化状况,及与赤潮生物的线性回归关系。分析结果表明,该海域出现高营养盐以至形成富营养化乃是江浙沿岸流和瓯江等河流径流及远岸区盐度锋辐聚的综合影响的结果。根据回归分析,NO_3-N,SiO_3-Si与浮游植物细胞密度之间存在着较为显著的负相关。亦即N,Si和高N/P比(35:1左右)可能是导致浮游植物(夜光藻)异常增殖的重要因素。  相似文献   

Shallow-Water Gasohydrothermal Vents of Ushishir Volcano and the Ecosystem of Kraternaya Bight (The Kurile Islands)   总被引：2，自引：0，他引：2  

V. G. Tarasov    M. V. Propp    L. N. Propp    A. V. Zhirmunsky    B. B. Namsakakv    V. M. Gorlenko  D. A. Starynin 《Marine Ecology》1990,11(1):1-23

Abstract. A marine ecosystem in the crater of the Ushishir Volcano (Kraternaya Bight, Yankich Island, the Kuriles) showing gasothcrmal activity was studied for hydrographical, hydrochemical, and biological characteristics. Maximal changes in chemical and biological characteristics were observed in the surface water layer of 0–5 m. This layer had higher water temperature, was saturated with volcanogcnic carbon dioxide (up to 2000 10^-6 torr), ovcrsaturatcd with oxygen (up to 200 %), and contained high concentrations of chlorophyll a. Hydrogen sulfide was found in the surface water layer and at a depth of 15 m in the area of maximal effect of volcanic effluents.
The planktonic community is characterized by high rates of production and destruction of organic matter only in the 0–5 m layer. Daily vertical migrations of the ciliatc Mesodinium rubrum were observed, which caused "red tides".
Bacterial, algobacterial, and diatom mats developed on the bottom of the bight in the zone of gasohydrothermal vents and in areas of volcanic water seeping. The rate of organic matter production in algobacterial mats reached 33.4g C-rrr²-d^-l, chcmolithotrophy predominating. Bottom settlements had high population density and great animal biomass, which reached 10 kg m^-2 in gasohydrothermal fields. They obtain sufficient energy from primary production of microphy-tobenthos, algobacterial, and bacterial mats. Bcnthic species dominant in the bight were not found off the Ushishir Islands.
Some species of macrobenthos inhabiting the Kraternaya Bight differ markedly in size and biomass from the same species found beyond the bight.  相似文献   

美国红鱼试养的初步研究     

毛兴华  季如宝  朱明远  王宇宁 《海洋科学进展》1997,(1)

笔者自行培育的美国红鱼苗体长25～30mm．1996年6月上、中旬到当年12月中旬．其个体的平均重量分别达到了750g和800g，生长速度优于我国其他养殖鱼种。  相似文献   

Theory and Practice of Soft Clay Engineering     

Wei Rulong Prof. Senior Engineer  Geotechnical Engineering Department  Nanjing Hydraulic Research Institute  Nanjing 《中国海洋工程》1994,(1)

-The shear strength and deformation properties of soft clay are discussed first. Then some methods for predicting the performance of soft clay foundation are proposed. Finally, case histories are presented to illustrate some discussed aspects of soft clay.  相似文献   

Physical influences on recruitment to California Current invertebrate populations on multiple scales   总被引：4，自引：0，他引：4  

Botsford  Louis W. 《ICES Journal of Marine Science》2001,58(5):1081-1091

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