南大洋深海嗜冷菌2-5-10-1及其低温脂肪酶的研究   总被引：14，自引：0，他引：14  

林学政  杨秀霞  边际  黄晓航 《海洋学报》2005,27(3):154-158

从南大洋普里兹湾的水样中筛选到一株产低温脂肪酶的深海嗜冷菌2-5-10-1,对其生长及产酶情况和酶性质做了初步研究.该菌株的最适生长温度为5℃,此时分泌的胞外脂肪酶最多;添加Tween80,橄榄油可显著促进脂肪酶的产生.该脂肪酶的最适作用温度为35℃,在0～20℃均保持较高的酶活性,在0℃可保持37%的相对酶活性;酶的最适作用的pH值为7.5,在pH6～9的范围内均存在较高酶活性;对热较敏感,在60℃保温15min可丧失50%以上的酶活性.该脂肪酶的催化作用不需要金属离子的参与,Cu2+和Zn2+对酶活有着强烈的抑制作用.  相似文献   

海洋细菌抑菌活性菌株的筛选   总被引：16，自引：2，他引：16  

骆祝华  黄翔玲  王琳  裴耀文  叶德赞 《台湾海峡》2002,21(2):181-186

从不同海区的海洋底质，生物样，水样中分离到480株海洋细菌，其中包括150株芽孢细菌和108株粪大肠杆菌，利用纸片法对其抑菌活性进行检测，结果表明：117株海洋细菌具有抑菌活性，占总分离菌株的24.4%；一般细菌，芽孢细菌，粪大肠杆菌的抑菌活性菌株分别占各供测菌株的17.1%,27.3%,35.2%；抑菌活性菌株的比例与样品来源密切相关，且各类海洋细菌的抗菌谱有很大的不同。  相似文献   

Assessment of the effects of hydrocarbon contamination on the sedimentary bacterial communities and determination of the polar lipid fraction purity: Relevance of intact phospholipid analysis     

Nicolas Mazzella  Josiane Molinet  Agung Dhamar Syakti  Jean-Claude Bertrand  Pierre Doumenq   《Marine Chemistry》2007,103(3-4):304-317

This in situ study reports on the relevance of intact phospholipids as new biomarkers and highlights the limits of the classical fatty acid analysis after the hydrolysis of the polar lipid fraction. The analysis of polar lipid fractions revealed significant contributions of marine macrophytes with either non-phospholipid compounds (sulfoquinovosyl diacylglycerol) or with both non-bacterial phospholipid classes (phosphatidylcholine) and non-bacterial phospholipid molecular species (some molecular species of phosphatidylglycerol). The analysis of intact phospholipids showed also the predominance of gram-negative bacteria in sediments whereas the analysis of fatty acids alone cannot confirm such information. Lastly, as regard to the effects of petroleum hydrocarbons on the sedimentary bacterial communities, we observed a higher degree of saturation of the fatty acyl chains of the phospholipids extracted from sediment exposed to a massive and chronic crude oil contamination. Such a result was interpreted as an adaptive response resulting in higher membrane rigidity of the bacterial communities.  相似文献   

Mussel Periostracum from Deep-Sea Redox Communities as a Microbial Habitat: 3. Secondary Inhabitants     

James E.  Hook Stjepko  Golubic 《Marine Ecology》1992,13(2):119-131

Abstract. Vacated microborings in periostracum of live mussels of the Florida Escarpment redox community (depth of 3266 m) become a habitat for a prolific secondary microbiota consisting of a variety of prokaryotic and a few eukaryotic organisms. Periostracum surface is also colonized by diverse microorganisms, dominated by presumed chemolithotrophic bacteria with stacks of intracellular lamellae. Unlike sheltered microflora within borings, the surface community is heavily grazed upon by numerous archaeogastropods and ciliates.  相似文献   

Application of Atomic Force Microscopy to Observation of Marine Bacteria   总被引：3，自引：0，他引：3  

Tomohiko Nishino  Eiko Ikemoto  Kazuhiro Kogure 《Journal of Oceanography》2004,60(2):219-225

The atomic force microscopy (AFM) is a recently developed, bench-top instrument that can image the surface structures of biological specimens at high resolution with simultaneous measurement of their size. This paper describes the application of AFM to marine bacteria. Both natural and cultured bacteria were retained on a filter or placed on glass, washed, air-dried and observed by AFM. The instrumental condition, the choice of suitable filter, effect of fixation and filtration, comparison with epifluorescent microscopic (EFM) count, and the size and shape of bacterial cells were investigated. An Isopore filter was best for concentration and subsequent observation because of its surface flatness. Cross section images showed that both rod and coccoid cells were flattened, the former usually having a two-humped shape. Bacterial cells were differentiated from non-living particles based on their cross section shape and size. Bacterial counts by AFM and EFM showed good agreement. Although size measurement is easily done by the instrument, AFM tends to overestimate the size of microspheres. More work is thus needed on the size measurement of living organisms. Because AFM easily provides images of natural bacterial cells at high magnification, it can be used as a new tool to study the fine structures of marine bacteria. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

秋季东、黄海异养细菌(Heterotrophic Bacteria)的分布特点   总被引：27，自引：1，他引：27  

赵三军  肖天  岳海东 《海洋与湖沼》2003,34(3):295-305

2000年10-11月,乘"北斗号"考察船进行秋季"东、黄海生态系统动力学与生物资源可持续利用"大面调查,研究秋季东、黄海异养细菌的分布.结果表明,异养细菌在黄海和东海的丰度分别在(2.37-13.33)×108 cell/L和(3.05-13.62)×108 cell/L之间.细菌丰度最高值出现在长江口附近,且断面E和断面F各站位的细菌丰度明显高于其他断面.异养细菌丰度大小以长江口为中心向外海依次递减.东、黄海水体异养细菌生物量分别在244.45-1812.90mgC/m2和100.60-940.87mgC/m2之间.东、黄海异养细菌丰度无显著差异,但是东海海域水体异养细菌生物量高于黄海海域.异养细菌空间分布与浮游植物叶绿素在东海有一定的相关性,黄海异养细菌与硝酸盐浓度的相关性极显著.  相似文献   

Biological Responses to Seasonally Varying Fluxes of Organic Matter to the Ocean Floor: A Review   总被引：8，自引：0，他引：8  

Andrew J. Gooday 《Journal of Oceanography》2002,58(2):305-332

Deep-sea benthic ecosystems are sustained largely by organic matter settling from the euphotic zone. These fluxes usually have a more or less well-defined seasonal component, often with two peaks, one in spring/early summer, the other later in the year. Long time-series datasets suggest that inter-annual variability in the intensity, timing and composition of flux maxima is normal. The settling material may form a deposit of “phytodetritus” on the deep-seafloor. These deposits, which are most common in temperate and high latitude regions, particularly the North Atlantic, evoke a response by the benthic biota. Much of our knowledge of these responses comes from a few time-series programmes, which suggest that the nature of the response varies in different oceanographic settings. In particular, there are contrasts between seasonal processes in oligotrophic, central oceanic areas and those along eutrophic continental margins. In the former, it is mainly “small organisms” (bacteria and protozoans) that respond to pulsed inputs. Initial responses are biochemical (e.g. secretion of bacterial exoenzymes) and any biomass increases are time lagged. Increased metabolic activity of small organisms probably leads to seasonal fluctuations in sediment community oxygen consumption, reported mainly in the North Pacific. Metazoan meiofauna are generally less responsive than protozoans (foraminifera), although seasonal increases in abundance and body size have been reported. Measurable population responses by macrofauna and megafauna are less common and confined largely to continental margins. In addition, seasonally synchronised reproduction and larval settlement occur in some larger animals, again mainly in continental margin settings. Although seasonal benthic responses to pulsed food inputs are apparently widespread on the ocean floor, they are not ubiquitous. Most deep-sea species are not seasonal breeders and there are probably large areas, particularly at abyssal depths, where biological process rates are fairly uniform over time. As with other aspects of deep-sea ecology, temporal processes cannot be encapsulated by a single paradigm. Further long time-series studies are needed to understand better the nature and extent of seasonality in deep-sea benthic ecosystems. This revised version was published online in August 2006 with corrections to the Cover Date.  相似文献   

鱼类消化道细菌及其作用研究Ⅱ.鲻鱼     

周宗澄  叶德赞  倪纯治  刘文华 《台湾海峡》1997,(1)

本文报道了从鲻鱼消化道分离的好气性异养细菌数量为（1．5×108±0．4×108～6.4×108±2.0×108）个／g，共分离220株细菌。其种群组成为弧菌、气单胞菌、不动细菌、假单胞菌、微球菌、黄杆菌、葡萄糖细菌、纤维单胞菌、节细菌和棒状杆菌。测定其生化特性，表明该类菌对有机质的分解能力比较强，这些特性有利于鱼类对营养物质的消化和吸收，并进一步促进其生长和发育。  相似文献   

Symbiotic Relations of Sediment-Agglutinating Nematodes and Bacteria in Detrital Habitats: The Enzyme-Sharing Concept   总被引：2，自引：0，他引：2  

Franz Riemann  & Elisabeth Helmke 《Marine Ecology》2002,23(2):93-113

Abstract. A new concept (‘enzyme sharing’) concerning the interaction of marine nematodes and microbes in the degradation of sedimentary detritus is presented. Elements of this concept are (1) the notorious tendency of many aquatic nematodes to agglutinate detrital particles by mucus secretions, (2) new observations on the stimulation of microbial growth by nematodes in agar plates, and (3) literature data on limited endogenous proteolytic capacities of aquatic nematodes.
Observations on nematode‐microbe associations in agar plates prompted the conceptual synthesis. In agar medium without the addition of any nutrients a spectacular growth of bacteria was visible on the sinusoidal crawling trails of nematodes only 2 – 3 days after introduction of the worms (species of Adoncholaimus, Anoplostoma and Sabatieria). Juveniles of Anoplostoma that hatched in the agar cultures left their minute trails in the medium and these were rapidly occupied by bacteria. The nematodes repeatedly visited their bacterial trails, which persisted as a peculiar biotic structure for more than one year and survived the nematodes.
In sterile agar preparations containing the fluorogenic methylumbelliferyl‐β‐glucoside in the presence of the nematode Adoncholaimus, an enhanced fluorescence of the medium was visible, indicating β‐glucosidase activity. We therefore assume that oncholaimid nematodes discharge enzymes that alone, or in concert with microbial activities, contribute to the hydrolytic cleavage of refractory polysaccharides containing β‐glucosidic bonds such as agar components and cellulose. The sugars thus produced may then be taken up by the nematodes and concomitantly support the conspicuous growth of microbes. Since we did not observe any feeding of the nematodes on the associated microbes in agar plates, we question the nutritive potential of intact microbial cells for a number of nematodes abounding in detrital habitats, and call attention to the significance of ambient dissolved or adsorbed organic monomeric nutrients.
Consequently, we perceive the puzzling perpetual accretion of detrital organic particles to sediment agglutinations by nematodes as an adaptation for operating an ‘enzymatic reactor’ for the production of dissolved nutrients. We hypothesise a relationship of mutual commensalism of nematodes and heterotrophic microbes in detrital habitats and propose the term ‘enzyme sharing’ for this relationship. Both parties invest in a common enzyme pool that decomposes organic detritus for their nutrition. We present here evidence that nematodes contribute β‐glucosidase, which is involved in the cellulase system. Data from the literature suggest that microbial enzymatic processing of detrital proteins yields amino acids available to nematodes, which apparently have no efficient proteolytic enzyme system in their intestines.  相似文献   

牙鲆(Paralichthys olivaceus)秦皇岛弧菌感染症及其病原细菌研究   总被引：1，自引：1，他引：1  

陈翠珍  张晓君  房海  靳晓敏  王秀云 《海洋与湖沼》2006,37(1):35-40

采用病原分离与鉴定、人工感染试验等方法,在对养殖牙鲆病例进行发病情况、病理变化特征检验的基础上,进行病原学检验。结果表明,此病例属于由弧菌属(VibrioPacini1854)细菌引起的败血感染症。经对12株纯培养菌(HQ010712-1—HQ010712-12)进行形态特征、理化特性等表观分类学指征的检验,选择代表菌株(HQ010712-1株)进行16S rRNA基因的测定与系统发育学分析等,判定为弧菌属的一个新种。将HQ010712-1株送中国典型培养物保藏中心(CCTCC)进行了复核鉴定与分类定名,依据分离地定名为秦皇岛弧菌(Vibrioqinhuangdaorasp.nov.);参考菌株为HQ010712-1。  相似文献