可口革囊星虫(Phascoloma esculenta)铁结合蛋白基因的研究   总被引：2，自引：2，他引：0  

杜莉利  李太武  苏秀榕  李登峰 《海洋与湖沼》2008,39(3):252-256

根据已报道的铁结合蛋白基因的保守序列设计引物,用cDNA末端快速扩增(RACE)法,成功地从可口革囊星虫体液中获得铁结合蛋白基因的全长序列.结果表明,该基因cDNA全长1017bp,5'-端非编码区为15lbp,3'一非编码区为341bp,开放阅读框长度为525bp(括一个终止密码子),可编码175个氨基酸(GenBank:EU091352).该序列与加洲海兔、皱纹盘鲍、刺参、微小牛蜱、叉尾鲴、非洲爪蟾、小家鼠、人等的铁结合蛋白基因有67%-75%的同源性,而相应的氨基酸同源性为59%-76%.氨基酸相似性为74%*-89%%.分析结果表明,铁结合蛋白基因在动物进化中是高度保守的.  相似文献   

台湾海峡多管水母属——新种及基于线粒体COI序列分析鉴定多管水母   总被引：1，自引：0，他引：1  

郑连明  林元烧  李少菁  曹文清  许振祖  黄加祺 《海洋学报》2008,30(4):139-146

报道了中国台湾海峡多管水母属一新种——台湾多管水母(Aequorea taiwanensis n.sp.),详细描述其形态特征,并基于线粒体COI基因片段序列,比较、分析了该新种和乳突多管水母、锥状多管水母的序列差异及遗传距离。形态学和分子学数据都支持台湾多管水母为多管水母属有效种。3种多管水母mtCOI基因片断序列在种间存在明显的多态性,3种多管水母的种间序列差异为9.10%~11.9%,种间遗传距离为0.097~0.130,说明该基因片段适用于多管水母属种、种以下阶元及地理种群甄别和种间系统进化学分析。结合其他学者研究结果,认为mtCOI序列差异10%~20%可以作为多管水母属及其他水螅水母纲动物同属种间差异的标准。  相似文献   

一株印度洋深海食烷茵(Alcanivorax sp.P40)的烷烃羟化酶基因的克隆     

赖其良  袁军  邵宗泽 《台湾海峡》2008,27(2):141-146

以印度洋深层海水,用柴油和石油作为混合碳源经富集培养、分离获得一株具有很强的柴油降解能力的茵株P40.茵株P40革兰氏染色阴性,接触酶和氧化酶为阳性,能还原硝酸盐,不能还原亚硝酸盐.16S rDNA Blastn 结果表明其与 Alcanivorax dieselolei B-5T(柴油食烷茵)及 A.dieselolei NO1A具有最高相似性,均为99.8%.茵株P40的gyrB序列与 A.dieselolei NO1A同源性也高达99.2%.而与A.dieselolei B-5T只有86.9%.此外,从茵株P40中克隆到两个烷烃羟化酶alkB基因片断,分别命名为P40-alkB1和P40-alkB2.其中P40-alkBl与报道的 A.dieselolei B-5T 中的 alkB 同源性较高,达96.3%,而与同为深海来源的茵株NO1A的alkB的同源性更是达100%,P40-alkB2则与A.borkumensis SK2T(泊库岛食烷茵)的alkB1同源性最高,但仅为65%.  相似文献   

海洋软体动物神经内分泌学的研究进展     

卢永忠 《海洋通报》2008,27(4)

主要从组成、研究方法、活性物质的种类和功能研究等方面对海洋软体动物神经内分泌的相关研究成果进行了介绍.  相似文献   

Construction and characterization of a fosmid library for pathogenic bacterium <Emphasis Type="Italic">Vibrio anguillarum</Emphasis>     

韩一凡  莫照兰  茅云翔  肖鹏  李杰  郝斌  郭东升 《中国海洋湖沼学报》2009,27(3):519-526

Vibrio anguillarum is a common bacterial pathogen in fish.However,little is known about its pathogenic mechanism,in part,because the entire genome has not been completely sequenced.We constructed a fosmid library for V.anguillarum containing 960 clones with an average insert size of 37.7 kb and 8.6-fold genome coverage.We characterized the library by end-sequencing 50 randomly selected clones.This generated 93 sequences with a total length of 57 485 bp covering 1.4% of the whole genome.Of these sequences,58...  相似文献   

Screening of eye-position related genes with DD-RT-PCR and RDA in the hybrids between Japanese flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus     

陈妍婕  张全启  齐洁  孙业盈  钟其旺  王旭波  王志刚  李朔  李春梅 《中国海洋湖沼学报》2009,27(1)

Flatfish or flounder moves one eye to change body proportion into vertebral asymmetry during metamorphosis, during which some become sinistral while others dextral. However, the mechanism behinds the eye-position has not been well understood. In this research, hybrids between Japanese flounder(♀) and stone flounder (♂) show mixed eye-location in both dextral type and sinistral type, and thus become good samples for studying the eye-migration. mRNAs from pro-metamorphosis sinistral and dextral hybrids larvae...  相似文献   

一株甲胺磷高效降解菌——巴氏葡萄球菌(Staphylococcus pasteuri)的筛选及其分子鉴定     

邓先余  高健  谭树华  向言词 《海洋与湖沼》2009,40(5):551-556

从湘潭南天化工厂的甲胺磷废水和污泥中分离细菌样品,以甲胺磷为唯一碳源和能源,经过定向筛选,得到1株可高效降解甲胺磷的菌株HN003,气相色谱测定其对甲胺磷的平均降解效率在24h、48h和72h分别达到45.8%、88.5%和100%。对其进行常规生理生化测试,结果表明,菌株HN003革兰氏染色为阳性,在10-42℃、pH3.0-pH14.0范围内均能生长,并与巴氏葡萄球菌的表型特征非常相似。为了进一步确定HN003的分类学地位,测定了其16S rRNA基因序列(1541bp),分析了相关细菌相应序列的同源性,构建了分子系统发育树,结果表明,菌株HN003与巴氏葡萄球菌的亲缘关系最近。综合上述结果,菌株HN003可鉴定为巴氏葡萄球菌(Staphylococcus pasteuri)。  相似文献   

棕囊藻渤海株核糖体18S rDNA和ITS基因结构序列分析   总被引：5，自引：0，他引：5  

曲凌云  吕颂辉  高春蕾  李艳  孙萍  孙修勤 《海洋科学进展》2008,26(2):200-206

对分离自渤海赤潮发生区的1株棕囊藻进行核糖体18S rDNA及ITS序列克隆测定,获得了长度为1 648 bp的18S rDNA序列和长度为890 bp的ITS序列.对获得的基因序列进行同源性分析,结果表明,该棕囊藻的核糖体18S rDNA及ITS序列均与NCBI数据库中登录的球形棕囊藻的相应序列同源性最高;从GenBank中获取不同地理来源的19种棕囊藻的18S rDNA序列及6种棕囊藻的ITS序列,分别以棕囊藻核糖体18S rDNA和ITS为对象构建了棕囊藻属的系统发育树,从分子生物学角度确定了棕囊藻渤海株为球形棕囊藻(Phaeocystis globosa).  相似文献   

Distribution and generic composition of culturable marine actinomycetes from the sediments of Indian continental slope of Bay of Bengal   总被引：3，自引：0，他引：3  

Surajit Das  P. S. Lyla  S. Ajmal Khan 《中国海洋湖沼学报》2008,26(2):166-177

Actinomycetes population from continental slope sediment of the Bay of Bengal was studied. Samples were collected during two voyages of FORV Sagar Sampada in 2004 （May-June） and 2005 （July） respectively from 11 transects （each transect had ca. 200 m, 500 m, and 1 000 m depth stations）. The physicochemical parameters of overlying water, and sediment samples were also recorded. The actinomycete population ranged from 5.17 to 51.94 CFU/g dry sediment weight and 9.38 to 45.22 CFU/g dry sediment weight during the two cruises respectively. No actinomycete colony was isolated from stations in 1 000 m depth. Two-way analysis of variance showed significant variation among stations （ANOVA two-way, P〈0.05）, but no significance was found between the two cruises （ANOVA two-way, P〈0.05）. Populations in stations in 500 m depth in both cruises were higher than that of 200 m depth stations with statistically insignificant difference （ANOVA two-way, P〉0.05）. Three actinomycetes genera were identified. Streptomyces was found to be the dominating one in both the cruises, followed by Micromonospora, and Actinomyces. The spore of Streptomyces isolates showed the abundance in spiral spore chain. Spore surface was smooth. Multiple regression analysis revealed that the influencing physico-chemical factors were sediment pH, sediment temperature, TOC, porosity, salinity, and pressure. The media used in the present study was prepared with seawater. Thus, they may represent an autochthonous marine flora and deny the theory of land runoff carriage into the sea for adaptation to the salinity of the seawater and sediments.  相似文献   

从细胞色素 b基因全序列研究鞍带石斑鱼的分类学地位   总被引：3，自引：0，他引：3  

区又君  吴勇  刘楚吾 《热带海洋学报》2008,27(3):45-49

通过对鞍带石斑鱼 Epinephelus lanceolatus、棕点石斑鱼E. fuscoguttatus、斜带石斑鱼E. coioides、鲑点石斑鱼E. fario的线粒体细胞色素b基因全序列扩增、克隆及测序,得到1 141bp序列,并结合GenBank其他7种石斑鱼同源序列进行比较分析,共发现429个核苷酸位点存在变异(37.6%).序列中的转换大于颠换,碱基替换多发生于密码子第3位.以4种鲈形目鱼类为外群,用MEGA3.0软件中的NJ、ME、MP等3种方法构建分子进化树,结果显示,鞍带石斑鱼与石斑鱼属鱼类有较近的亲缘关系,支持鞍带石斑鱼归入石斑鱼属.  相似文献