4种重金属离子对海洋三角褐指藻生长影响的研究   总被引：23，自引：0，他引：23  

张首临  刘明星  李国基  包万友  顾宏堪 《海洋与湖沼》1995,26(6):582-585

于１９９３年４月－１９９３年５月以Ｚｎ＾２＋，Ｃｄ＾２＋，Ｐｂ＾２＋，Ｃｕ＾２＋不同浓度的海水对三角褐指藻进行生长影响的研究。实验表明，Ｃｕ＾２＋对三角褐指藻生长的毒性最大，其９６ｈＥＣ５０为０．０１７ｍｇ／Ｌ。Ｐｂ＾２＋的毒性最小，其９６ｈＥＣ５０为０．４６８ｍｇ／Ｌ。４种重金属离子的毒性顺序是Ｃｕ＾２＋＞Ｃｄ＾２＋＞Ｚｎ＾２＋＞Ｐｂ＾２＋，除Ｃｄ＾２＋外，其他３种金属离子对该单细胞藻的生长安全  相似文献   

河口变形效应与涌潮的形成     

杜勇 《中国海洋大学学报(自然科学版)》1989,(3)

作者采用非线性潮波方程,假定河口的宽度B(x)和深度h(x)是任意可微函数,导出了涌潮发生的条件,并讨论了各种变形情况下涌潮的形成。得出结论是,在涌潮形成过程中起决定作用的是浅水非线性效应,而河口变形效应则是起促进作用的。  相似文献   

江苏省主要湖泊的降水量与湖泊的降水效应   总被引：10，自引：0，他引：10  

陆鸿宾 《海洋湖沼通报》1991,(2):23-30

本文分析了江苏境内主要湖泊的降水量特征,揭示了长江是江苏各湖泊不同降水量特征的一条十分明显的天然界线;估算了太湖的降水效应,并据此推估出江苏主要湖泊的降水效应不太明显;江苏各湖泊的降水量与其水位年过程趋势相似,只是后者位相滞后于前者而已。  相似文献   

Rapid detection of virulent protease secreted by Vibrio anguillarum by dot enzyme-linked immunosorbent assay     

ZHANG Zhendong  ZHANG Peijun  MO Zhaolan  WANG Chunling  YU Yang 《海洋学报(英文版)》2005,24(4):155-161

Dot enzyme-linked immunosorbent assay （dot-ELISA）, indirect ELISA and Westem blot were performed to detect the virulent protease secreted by Vibrio anguillarum which was isolated from the diseased left-eyed flounder, Paralichthys olivaceous. Sensitivity results showed that dot-ELISA is a more sensitive, rapid and simple technique for the protease detection. The minimal detectable amount of protease is about 7 pg in the dot-ELISA test, while 7.8 ng in the indirect ELISA and 6.25 ng in the Westem blot respectively. Protease could be detected 2 h after incubation of V. anguillarum in the 2216E liquid medium but enzyme activity was very low at that period. From 6 to 12 h, the amount and enzyme activity of protease increased markedly and reached maximum at stationary phase. Analysis of serum samples periodically collected from the infected flounders showed that after 2 h of infection by V. anguillarum, the pathogenic bacteria could be detected in the blood of the infected flounders but no protease was found. It was 5-6 h after infection that the protease was detected in blood and then the amount increased as infection advanced. Quantitative detection of protease either incubation in the medium or from the blood of infected flounders could be accomplished in virtue of positive controls of quantificational protease standards （＂marker＂） so that the alterations ofprotease secretion both in vitro and in vivo could be understood generally. In addition, the indirect ELISA and dot-ELISA were also performed to detect V. anguillarum cells. Results indicated that the sensitivity of indirect ELISA to bacteria cells is higher than that of the dot-ELISA, and that the minimal detectable amount is approximately 10^4 cell/mL in the indirect ELISA, while 10^5 cell/mL in the dot-ELISA.  相似文献   

养殖中国对虾育苗效果观察     

李健  孙修涛  高成年  赵法箴  侯明泉  于成鸿 《海洋科学》1992,16(6):18-22

我国自80年代初大规模开展中国对虾(Penaeus chinensis)增养殖生产以来,每年需从自然海区捕捞大量对虾亲体用于人工育苗生产,为了保护自然资源,近年来我国采取了一系列措施,鼓励用人工养殖对虾亲虾越冬来解决亲虾来源。至1990年,仅北方三省一市利用越冬虾培育虾苗数量已达1.07×10~(10)尾,占养殖用虾苗总量的30％。预计今后几年内,这个比例还将增加。连  相似文献   

8种有机磷农药对卤虫毒性的研究   总被引：5，自引：0，他引：5  

马英杰  刘珊红  汝少国  董玉童 《海洋科学》1997,21(6):5-6

  相似文献   

茶皂素对几种鱼类和对虾的毒性研究   总被引：7，自引：0，他引：7  

梁亚全  夏春华  朱全芬  孙修涛  唐丽萍 《海洋科学》1992,16(3):20-25

本研究以茶皂素类作为“清池剂”,试图解决对虾养殖的基础和应用技术问题。试验结果表明:对虾对茶皂素和油茶皂素有较高的耐受力;茶皂素与油茶皂素均具鱼毒活性,油茶皂素则较强。鱼种不同,中毒反应也略有差异;两种茶皂素的鱼毒速度快,在致死浓度时16h内出现中毒死亡,毒性降解也快,浓度为5×10~(-6)时40h后不再引起鱼类中毒。  相似文献   

二色桌片参酶解液对转化细胞的效应   总被引：3，自引：2，他引：3  

陈正明  陈玉强  曹顺达  吴乔  苏文金 《台湾海峡》1997,16(3):270-274,T001

用二色桌片参（Mensamariaintercedens）酶解液处理转化的人胚肺成纤维细胞（NLF）后，细胞生长受到显著抑制，抑制率达64．84％，光镜下细胞透光性加强，铺展较好，细胞核形更为规则，核畸形现象减少；线粒体结构趋于正常；软琼脂集落实验表明，细胞集落形成率由23ｘ10－5下降至12．5ｘ10－5，裸小鼠致瘤实验显示抑瘤率为82．39％，差异显著（p＜5％）。结果提示，二色桌片参酶解液可能逆转转化的HLF细胞的恶性表型，对其有一定的诱导分化作用。  相似文献   

蒽胁迫对2种海洋微藻的毒性效应     

田继远  唐学玺  于娟  王晨  刘文明 《中国海洋大学学报(自然科学版)》2002,32(6):919-925

采用水生毒理学方法以及通过分析蒽对 2种海洋微藻的几种生理生化指标的影响 ,初步研究了蒽胁迫对 2种海洋微藻的毒性效应。结果表明 :随着蒽浓度的不断增大 ,1 2种海洋微藻的相对增长率逐渐降低 ,叶绿素 a和类胡萝卜素含量稍有下降 ,蒽对小新月菱形藻生长的 72 h· EC50为 2 51μg· L-1 ,蒽对亚心形扁藻生长的 72 h· EC50 为 387μg· L-1 ;2还原型谷胱甘肽 (GSH)含量逐渐降低 ;3加入外源性抗氧化剂 (GSH和甘露醇 )可缓解蒽胁迫所造成的 2种海洋微藻的细胞密度的降低 ,即可缓解蒽胁迫对微藻的毒害作用  相似文献   

东海大规模赤潮危害影响的实验研究——对黑褐新糠虾和卤虫的毒性效应     

颜天  张艺  韩刚  陈洋  周名江 《海洋科学集刊》2007,(48):166-175

The effects of Prorocentrum donghaiense,Karenia mikimotoi,Alexandrium catenella,the causative species of Harmful Algal Blooms in the East China Sea,on the survival and growth of Neomysis awatschensis and Artemia salina were investigated.And the toxicity of eleven strains of Alexandrium----A.lusitanicum(AL-1)、A.minutum(AM-1)、A.spp.(A-K)、A.tamarense(ATHK,ATCI02,ATCI03,AT5-1,AT5-3,AT-6)、A.affine(AS-1,AC-1) to the two above organisms were compared. The results showed that P.donghaiense and K.mikimotoi had no adverse effect on the survival and growth of N.awatschensis and A.salina at 96h.However,the survival rate of N.awatschensis and A.salina declined linearly with the increasing density of A.catenella,and the 96h LC₅₀ for N.awatschensis and A.salina was 4500 and 460 cells/mL,respectively, Five strains of Alexandrium----A.spp,A.lusitanicum (AL-1),A.tamarense(AT5-3,AT-6) and A.affine (AS-1)at the density of 4000cells/mL and 96h had no adverse impacts on N.awatschensis. But other six strains of Alexandrium a.Tamarense(AT5-1,ATHK,ATCI02,ATCI03),A.affine(AC-1) and A.minutum(AM-1)decreased the survival fate of N.awatschensis significantly. Two strains of Alexandrium----A.lusitanicum(AL-1) and A.tamarense(AT-6),at the density of 500cells/mL. had no adverse effects on A.salina in 96h;while the other Alexandrium strains all had adverse effeets on the survival of a.salina, A.salina could increase its length with the increasing density of P.donghaiense,K.mikimotoi and A.luxitanicum. The results indicate that P.donghaiense and K.mikimotoi have nio significant effect on the survival of crustaeean zoop lankton in a short period. But several Alexandrium specised/strains could influence the survival of crustacean zooplankton likely by non-PSP substances. A.salina is shown to be more sensitive than N.awatschensis as test organisms to harmful species.  相似文献