卫矛醇对C6胶质瘤细胞体外抗癌活性及机制探讨          下载免费PDF全文

张玉岭  张涛  陈宝贵 《应用海洋学学报》2024,40(2):168

目的:研究卫矛醇对C6胶质瘤细胞株的体外增殖及凋亡的影响。方法：将C6大鼠胶质瘤细胞随机分为对照组、卫矛醇干预组、卫矛醇+雷帕霉素组、卫矛醇+3-MA组,各组予不同药物孵育,采用MTT法检测卫矛醇对C6细胞的体外增殖抑制率,筛选出抑制率相对较高且生长状态良好的细胞;应用Hoechest 33342染色透射电镜观察细胞凋亡亚显微结构的改变,采用免疫荧光和Western Blot技术检测与凋亡和自噬相关蛋白的表达水平。结果:卫矛醇对C6胶质瘤细胞增殖有明显的抑制作用,并呈现出良好的浓度-效应相关性,与对照组比较,随着浓度的增加,细胞的增殖抑制率上升(P＜0.01);Hoechst染色结果显示,与对照组相比,共同孵育卫矛醇24 h后的C6细胞出现明显的核固缩现象,证明有大量的细胞出现凋亡;Western Blot结果显示,与对照组相比,促凋亡基因Bax表达上调,抗凋亡基因Bcl-2表达下调。结论:卫矛醇能够抑制C6细胞的增殖,并诱导其凋亡,其作用机制可能与上调Bax、下调Bcl-2有关。  相似文献   

Early diagenesis of nurtrients （C, N, P and Si） stored in sediments of two reservoirs in southwestern China     

Yuchun WANG Jun ZHU Congqiang LIU 《中国地球化学学报》2006,25(B08):161-162

Damming is a common anthropogenic intervention along the course of rivers, which is defined as ＂artificial-lake effect＂, both in China and across the world. Today as many as 48000 dams and/or reservoirs are in operation in the Changjiang River （Yangtze River） drainage area, and more are being constructed. While damming is well known to affect riverbome nutrient loads, and thus the riverine ecosystems owing to removal of carbon fixation, and removal of particles in reservoir sediments, there is limited information on the detailed early diagenesis of sediments in reservoirs including the regeneration processes of nutrients deposited in sediments and exchange flux across the sediment-water interface, which is important for mass balance of riverbome nutrients. In the present study, two large-size reservoirs, Wujiangdu Reservoir （WJDR） and Dongfeng Reservoir （DFR）, located on the main steam of the Wujiang River and with uniformity hydrography and discrepancy biogeochemical activity （e. g. primary production）, were selected for a comparative study on the detailed processes of nutrient regeneration. Water, pore water and sediment were sampled from these two reservoirs and dissolved organic carbon （DOC）, NH4^＋, NO3^-, PO4^3-, dissolved silica （DSi） in the overlying water and pore water, and total organic matter in sediments were determined. The results of correlation analysis suggested that in these two reservoirs, processes of nutrient regeneration near the sediment-water interface were significantly different. As a result of rapid decomposition of algae-derived ＂labile＂ organic matter in upper sediments, nutrient regeneration processes and upward fluxes in WJDR are dramatically stronger than those of DFR. NH4^＋ upward flux from sediment in WJDR was about 17 times higher than that in DFR. PO4^3- flux in WJDR is about 13 times above that in DFR. DOC flux in WJDR is larger that in DFR by 5 times, and DSi by 1 time.  相似文献   

Response of Ruppia maritima to the reclaimed wastewater in coastal artificial landscape river     

Weihong WANG Min JI Yuwei XUE 《中国地球化学学报》2006,25(B08):167-168

In recent years, reclaimed wastewater as a water source for urban artificial landscape rivers is being rapidly developed in some coastal cities with limited water resource available in China. However, eutrophication occurs more often in the landscape waterbody with reclaimed wastewater than in natural rivers and lakes. The study of restoration and reconstruction of macrophytes, especially the submerged aquatic vegetation, is essential to inhibit eutrophication of reclaimed wastewater. This study investigated the seasonal variation of biomass of Ruppia maritima in the artificial landscape river using reclaimed wastewater as the only water source in Tianjin, China. It was found that there were two seasonal growth peaks of Ruppia maritima each year in the coastal reclaimedwastewater. The contents of nitrogen and phosphorus of Ruppia maritima varied with the season.  相似文献   

福建省山仔水库浮游植物特征与水体富营养状况分析     

苏玉萍  陈娜蓉  林婉珍  梁晓丹 《福建地理》2006,(4)

采用浮游植物指示法评价了福州省山仔水库2005年不同季节水体的富营养状况,结果表明:冬、春季库区水体处于中—富营养水平,夏季发生水华微囊藻(Microcystis flos-aquae)“水华”,水体处于富营养水平,秋季水体处于中营养水平.由于浮游植物的生长状况受到多因素的影响,有必要进一步结合其他评价方法对整个水体的富营养状态做出综合判断.  相似文献   

固体介质中弹性波传播机制的细胞自动机有限深势阱模型     

赵雪平  杨宝俊 《地球物理学进展》2002,17(3):390-398

分析了采用细胞自动机研究波动问题的建模方法，针对一维、均匀、各向同性固体介质中弹性纵波的微观机制，借用一经典弹簧振子模型、细胞自动机格子气模型，以及量子力学中的无限深势阱模型，建立了一个细胞自动机有限深势阱模型，从量子力学角度出发，基于介观物理和纳米概念，以微观精子的德布罗意假设为基础，利用薛定谔方程，讨论了该模型中粒子（分子组）的振动速度与粒子物质波波速之间的联系，给出了模型中的波动方程，得出ζ＝Vp（ζ为粒子振动速度，Vp为物质波纵波波速）。同时还讨论了模型中粒子的大小和能量传递问题，引入引力场，得出了能量及引力势的量子化条件，另外，对声波速度、格子气粒子振动速度和本文模型中分子组振动速度进行了比较；还对本文模型中的粒子能量分布作了分析。  相似文献   

两个相连湖泊的微生态系统——云南高原湖泊中浅水的星云湖和深水的抚仙湖研究   总被引：4，自引：0，他引：4  

大久保贤治  熊谷道夫  坂本充  宋学良  张子雄 《云南地理环境研究》2002,14(2):10-19

抚仙湖是中国云南省的一个深水湖，连接着富营养化的浅水湖－－星云湖，星云湖排放含绿藻的水进入抚仙湖。两湖位于省会昆明附近，由于地方文化、集约化农业、以及旅游业的发展，两湖已经富营养化。因为湖泊体积容量的级别差异，深水湖的富营养化几乎没被注意，抚仙湖秋季下层滞水带缺氧已经关注了20年，似乎下层滞水带是深水湖富营养化的指标或趋势。抚仙湖看起来目前情况尚好，可以说寡营养，然而，外界负荷是潜在的，且是以稳定的加速度进行的。在此对浅水湖富营养化的原因进行了讨论，包括在其它云南湖泊观察到富营养化的过程，再者，对两个连相湖的藻和蓝藻碎片的大小构成进行比照。为了与深水的抚仙湖状况进行比较，简略介绍了一个日本的深湖及其径流系统。其中，涡流和它的微生态系统，在两者中相关性很好。对于以涡流生态系统的见解判断微生态系统的深水湖富营养化问题将给予概括。  相似文献   

我国贫营养泥炭沉积的物质组成、理化特性及水化学环境分析     

冷雪天  王升忠  赵红艳  林庆华  洪冰  王羽 《矿物岩石地球化学通报》2003,22(2):114-119

泥炭藓是贫营养泥炭类型中的建群植物，由于生态水环境主要靠大气降水补给，少受其他水源的污染，可自然反映古降水、古湿度的情况，所以是古气候研究最理想的纤维素同位素测试材料之一。因此，弄清我国贫营养泥炭沉积的物质组成及水化学环境特征，具有重要意义。笔根据多年来的国内外野外调查，结合收集到的资料，以东北大小兴安岭山地的泥炭辞贫营养泥炭地为基础，与美国、加拿大以及俄罗斯等国家的同类泥炭地相比较，对我国贫营养泥炭沉积的物质组成、理化特性及水化学环境做了系统的分析研究，并就此讨论了湿地保护中的一些实际问题，以及相应的对策。  相似文献   

Isotopic Tracing of Trophication Processes over the Last Millennia on Lake Chenghai     

吴敬禄  王苏民  夏威岚 《中国地球化学学报》2003,22(3):271-279

Multi-parameter studies (stable isotopes in carbonate and organic matter, pigment,organic carbon and nitrogen contents) from a 660-yr continuous sediment core from Lake Cheng-hai, a closed, eutropic lake in southern China, provide information on lake historical eutrophi-cation. During the last 660 years, great changes have taken place in productivity and eutrophi-cation of Lake Chenghai in response to human activities. In 1690, the productivity of the lakebegan to increase as Lake Chenghai became closed from agriculture in the lake‘‘ s watershed. In 1942, Lake Chenghai evolved to eutrophic state, marked by an increase in organic carbon, ni-trogen, CaC03, pigment contents and obvious negative values of stable isotopes, which is more or less simultaneous with the large-scale population immigration during the period. In 1984, in-tensive human activities induced modern lacustrine productivity and eutrophic level. Human-in-duced trophic changes during the past few decades have affected the Lake Chenghai ecosystem tosuch an extent that it has never experienced before in the last 660 years.  相似文献   

高放废物处置系统地下水同位素特征   总被引：4，自引：1，他引：4  

郭永海  刘淑芬  吕川河 《地球学报》2003,24(6):525-528

在高放废物处置库场地特性评价中，对于深地质处置方案而言，最有可能使放射性核素进入生物圈的机制是地下水的迁移，因此，水文地质研究是场址预选和评价中的一个重要内容。本文以甘肃北山预选区旧井地段为例，根据地下水同位素数据，结合所获得的地质、水文数据和信息，分析研究了研究区基岩地下水的起源、形成、演化和循环特征。研究表明，北山预选区旧井地段，深部地下水和浅部地下水均源自大气降水的补给，弱含水、低渗透、低流速是该区的主要水文地质特征。  相似文献   

基于图像区域特征的细胞识别方法及实现   总被引：5，自引：0，他引：5  

程勇  傅德胜 《南京气象学院学报》2003,26(5):712-716

先提取目标物体的面积、中心矩、圆形度、细长度、核浆比等区域特征。然后采用单原型模式表征方法，利用集群分析技术中最小距离分类器，构造判别函数，并依此作为识别类型的依据。最后，通过对鳞细胞的区域特征进行计算比较，选择出能够较明显地辨别出类别的特征向量，建立判别函数并对结果的可信度进行了讨论。  相似文献