斑节对虾虾苗白斑综合症杆状病毒的检测和养殖跟踪   总被引：1，自引：0，他引：1  

邱德全  谢数涛 《广东海洋大学学报》1999,19(4):11-15

利用２步ＰＣＲ检测技术对湛江和阳西地区的虾苗进行白斑综合病杆状病毒（ＷＳＳＶ）检测。在仔虾２日龄最早检测得到病毒，有２５％的虾苗带有ＷＳＳＶ病毒。带病毒虾苗和不带病毒虾苗分别在不同养殖模式的养殖过程中跟踪。前者在湛江湖光镇普通虾塘跟踪养殖，它们在变化的环境中容易发病，ｐＨ、盐度和温度是重要的诱发因子，在养殖５０～６０ｄ时发病死亡；后者在高位池和普通池养殖跟踪，它们对变化的环境有较大的适应性，养殖时间为８０～１１０ｄ，在相对优良的养殖技术条件下大部分可望养殖成功。环境中有ＷＳＳＶ病原传入，不带病毒虾苗在养殖后期可以带有ＷＳＳＶ病毒，出现白斑虾。跟踪有普通池有爆发病害，但是时间延后，跟踪的高位池没有爆发病害。  相似文献   

基因转移技术简介     

喻达辉 《广东海洋大学学报》2000,20(3):80-86

应用物理、化学或 (和 )生物学等技术和方法将外源基因转移到受体菌或细胞内 ,并使之在细菌或细胞内实现转入基因的扩增和表达称基因转移技术。1　基因转移的物理学方法1 .1　显微注射法应用玻璃显微注射仪器 ,在显微镜下将外源 DNA直接注射入靶细胞的核内。该法的受体细胞主要是体积较大的受精卵细胞 ,是制备转基因动物的常用技术之一。Gordon1 980年报道了用显微注射制作转基因小鼠 ,在出生的 78只小鼠中 ,用 Southern法检测有 2只为阳性 [1]。可惜的是未得到活的转基因小鼠。 1 982年 Palmiter报道了将大鼠的生长激素基因用显微注射法…  相似文献   

野生二粒小麦 Triticum dicoccoides抗杀锈病基因 YrH52分子定位的初步研究(英文)     

PengJ.H.  FahimaT.  RoderM.S.  LiY.C.  DahanA.  GramaA.  NevoE. 《广东海洋大学学报》1999,(4)

主要对以色列野生二粒小麦赫尔蒙种群中分离获得的一个抗条锈病基因进行了分子定位研究 ,将源于赫尔蒙山具抗杀锈病的种系 T.dicoccides H52与普通的栽培种 Langdon进行杂交并创建了 F2 代遗传图。研究发现 H52种系抗条锈病的能力由一种显性基因控制 ,将其暂定名为 Yr H52。从 1 2 0个微卫星标记中 ,已经检测到来自亲本 91 %的多态性 ,而且从其中 56个微卫星分子标记中产生了 79个分离的位点 ,有 9个位点显示出了与 Yr H 52基因连锁 ,其重组率 0 .0 2～ 0 .3 5,遗传距离 2 .0 0～ 4 3 .3 7cm之间 ,L OD值 3 .56～ 54.2 2。由 1 0个微卫星位点和 Yr H52构建的染色体 1 B遗传图 ,其图距全长为 1 0 1 .5cm。Yr H52基因位于 Xgwm2 64 a和 Xgwm2 64 c之间 ,且与 Xgwm2 64 a、Xgwm1 8紧密连锁 ,两侧依次分别与 Xgwm1 3 1 a、Xgwm63 6b、Xgwm2 64 c、Xgwm4 0 3 a、Xgwm1 53、Xgwm550 a和 Xgwm1 2 4连锁。同时 ,Yr H52也与 REL P标记物 N or1紧密连锁 ,图距 1 .4 cm,L OD2 9.62。这显然与野生二粒小麦另一个抗条锈病基因 Yr1 5不同 ,研究证明 Yr1 5与 N or1图距是 1 1 .0 cm。  相似文献   

欧洲鳗鲡肝肾病病原菌的分离与鉴定   总被引：1，自引：0，他引：1  

吴斌  樊海平  曾占壮  张新艳  王凡 《中国海洋大学学报(自然科学版)》2010,40(11)

患肝肾病的养殖欧洲鳗鲡(Anguilla anguilla)出现肝、肾肿大,且肝脏具白色溃疡灶的症状。从肝脏中分离、纯化到优势细菌,定名AL60306NA1。人工感染试验证实:向欧洲鳗鲡腹腔内注射1.0×108cfu/mL菌株悬液,实验鱼死亡率达100.0%;注射1.0×107cfu/mL,死亡率为60.0%;形态学观察、API20E细菌鉴定试剂盒鉴定实验证明菌株AL60306NA1有动力、拥有革兰氏反应阴性、氧化酶阴性、H2O2酶阳性、兼性厌氧、发酵葡萄糖产酸产气、不发酵蔗糖和蜜二糖及L一阿拉伯糖、赖氨酸脱羧酶阳性、柠檬酸盐弱阳性、产生硫化氢和吲哚、MR阳性和典型的β-溶血等特征;16S rRNA特异性基因分析鉴定实验说明克隆的AL60306NA1 16S rRNA基因部份序列为1507bp、与迟缓爱德华氏菌(AB050829.1)的同源性达99.7%。综合上述结果,确定该菌株为养殖欧洲鳗鲡肝肾病的病原菌,并鉴定为迟缓爱德华氏菌(Edwardsiella tarda)野生型。用菌株AL60306NA1感染小白鼠实验测定该菌对小白鼠的LD50为7.1×105cfu/mL,同时自该菌株PCR扩增到长度约1000bp的溶血素基因特异性片段,结果表明菌株AL60306NA1有一定的致病力。  相似文献   

栉孔扇贝不同组织SOD、CAT和MPO活力的比较分析   总被引：4，自引：0，他引：4  

费来华  邵雁群  李赟 《海洋湖沼通报》2008,(3)

对2龄和3龄栉孔扇贝外套膜、鳃、消化盲囊、肾、闭壳肌、性腺组织超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和髓过氧化物酶(MPO)活力分析表明,检测组织均存在这三种酶,且在不同组织中活力表现存在明显差别,其中肾组织SOD、CAT活力最高,而MPO在消化盲囊中表现最高活力,与其它组织相比均呈显著性差异(P＜0.05).不同贝龄的栉孔扇贝酶活力比较表明,三龄贝各组织SOD、CAT和MPO活力均高于二龄贝.  相似文献   

THE ULTRASTRUCTURE OF SEPARATED AND CULTURED CELL OF PORPHYRA YEZOENSIS     

梅俊学  费修绠 《中国海洋湖沼学报》2001,19(1):35-39

There are many reports that cells (protoplasts) separated from the thallus of Porphyra by enzyme can develop to normal leafy thalli in the same way as monopores. But there are few investigations on the subcellular structure of the isolated vegetative cell for ccmparison with the subcellular structure of monospores. To clarify whether the separated and cultured cells undergo the same or similar ultrastructure changes during culture and germination as monospores undergo in their formation and germination, we observed their ultrastructure, ccxnpared them with those of the monospore and found that the ultrastructure of separated and cultured cells did not have the characteristic feature as that of monospore formarion, such as production of small and large fibrous vesicles, but was acccxnpanied by vacuolation andstarch mobilization like that in monospore germination. The paper also discusses the relations between monospores and separated and cultured cells.  相似文献   

Analysis on promoter elements of cpc operon from Arthrospira platensis     

LU Yongzhong  ZHANG Xuecheng 《海洋学报(英文版)》2008,27(1):85-91

  相似文献   

A unified C-band and Ku-band geophysical model function determined by neural network approach     

ZOU Juhong  LIN Mingsen  PAN Delu  CHEN Zhenghu  YANG Le 《海洋学报(英文版)》2008,27(6):33-39

The geophysical model function （GMF） describes the relationship between backscattering and sea surface wind, so that wind vec- tors can be retrieved from backscattering measurement. The GMF plays an important role in ocean wind vector retrievals, its performance will directly influence the accuracy of the retrieved wind vector. Neural network （NN） approach is used to develop a unified GMF for C-band and Ku-band （NN-GMF）. Empirical GMF CMOIM and QSCAT-1 are used to generate the simulated training data-set, and Gaussian noise at a signal noise ratio of 30 dB is added to the data-set to simulate the noise in the backscat- tering measurement. The NN-GMF employs radio frequency as an additional parameter, so it can be applied for both C-band and Ku-band. Analyses show that the %predicted by the NN-GMF is comparable with the σpredicted by CMOIM and QSCAT-1. Also the wind vectors retrieved from the NN-GMF and empirical GMF CMOIM and QSCAT-1 are comparable, indicating that the NN-GMF is as effective as the empirical GMF, and has the advantages of the universal form.  相似文献   

长江口沉积物-水界面砷的迁移转化机制与微生物调控分析     

张玉婷  段丽琴  宋金明  张乃星  尹美玲  李学刚  袁华茂 《地学前缘》2022,29(4):144-155

为探析长江口沉积物-水界面砷的迁移转化机制,本文分析了2019年夏季长江口4个站位上覆水和间隙水中总As浓度及形态的剖面变化特征,耦合氧化还原敏感元素(Fe、Mn和S)的剖面变化剖析了沉积物-水界面砷循环的Fe-Mn-S控制机制,同时结合砷相关功能基因探讨了沉积物-水界面砷迁移转化的微生物调控过程,估算了沉积物-水界面总As的扩散通量。结果表明,除A7-4站位外,长江口其他3个站位间隙水总As以As³⁺为主要存在形态,且总As浓度均在上覆水中为最低值(0.748~1.57 μg·L^-1),而在间隙水中随着深度增加而逐渐增加并在6~9 cm深度达到峰值(7.14~26.9 μg·L^-1)。间隙水总As及As³⁺浓度的剖面变化趋势与溶解态Fe²⁺、Mn²⁺相似,其均在中间层出现高值,说明沉积物Fe/Mn还原带砷的释放可能是随固相Fe(Ⅲ)或Mn(Ⅳ)的还原而转移到间隙水中的。氧化层和Fe/Mn还原带过渡区间隙水砷浓度与砷异化还原菌功能基因arrA和arsC丰度存在对应关系(除A1-3站外),说明砷异化还原菌将溶解As⁵⁺或固相As⁵⁺还原为溶解As³⁺可能是该过渡层砷迁移转化的另一重要过程。硫酸盐还原带的间隙水总As和As³⁺浓度降低,但由于间隙水的低S^2-浓度不利于砷硫化物生成,因此深层间隙水砷可能与铁硫矿物结合而被移除。底层环境氧化还原条件是影响沉积物-水界面砷迁移转化的重要因素,随底层水DO浓度的降低,砷迁移转化更倾向于微生物还原控制。长江口沉积物-水界面总As的扩散通量为1.18×10^-7~2.07×10^-7 μmol·cm^-2·s^-1,均表现为沉积物间隙水中总As向上覆水释放,即沉积物是研究区域水体总As的来源之一。  相似文献   

Genetic study of Kelp “901” strain     

夏鹏王秀良  李晓捷赵玉山姚琳  段德麟 《中国海洋湖沼学报》2005,23(2):152-157

Based on DNA extraction and optimization of random amplified reaction (RAPD) to the gametophytes and sporophytes of Kelp “901” strain, genetic study on variation was conducted to its parents and offsprings of F6, F7,Fs, and F9 generation. RAPD results have shown that among 30 selected primers for gametophytes, 297 loci ranging from 200 to 3 000 bp were obtained in the average of 9,9 loci for each primer, This indicated a high polymorphic rate with RAPD detection. UPGMA (unweighted pair-group method arithmetic average) analysis showed that each male and female gametophyte of a generation could be clustered into one pair separately. The genetic distances of the Kelp 901 generation were 0.321 2-0.476 7, and the maximum was between F7 and Fs (0.476 7). Identity analysis showed that F6 generation was more close to the female parent (0.659 3), and F7 generation was more close to the male parent (0.5788). To the sporophytes study in 24 selected primers for RAPD amplification, 191 loci ranging from 230-2 800 bp were obtained, in the average to each primer of 8.0 loci. The heterozygosity to six populations were male parent (0.223 9),female parent (0.107 2), F6 (0.216 4), F7 (0.228 6), F8 (0.229 6) and F9 (0.317 2). The nearest genetic distance was 0.083 5 (Fs, F9). Total heterozygosity (HT) ofF6, F7, F8 and F9 generations was 0.318 6, the average heterozygosity (Hs) for F6,F7, F8 and F9 generations was 0.248 0, and deduced coefficient of population differentiation (Gst) was 22.2%. Six sequence characterized amplified regions (SCAR) were preliminary screened through RAPD analysis. It needed to be verified in detail as they are significant for molecular marker assistance in breeding and selecting Laminaria.  相似文献