Optical spectroscopy of X-Mega targets – IV. CPD – 59°2636: a new O-type multiple system in the Carina Nebula     

J. F. Albacete Colombo  †‡  N. I. Morrell  §¶  G. Rauw  M. F. Corcoran  &#;  V. S. Niemela †  H. Sana †† 《Monthly notices of the Royal Astronomical Society》2002,336(4):1099-1108

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Distributions and assemblages of microbial communities along a sediment core retrieved from a potential hydrate-bearing region offshore southwestern Taiwan     

《Journal of Asian Earth Sciences》2014

Assessing the impacts of methane released from hydrate-bearing environments on global carbon cycling would require detailed insights into the distributions and capacities of microbial communities at different horizons of sediment column. In this study, we conducted geochemical, gene abundance and diversity analyses for a sediment core retrieved from a potential hydrate-bearing region off southwestern Taiwan. Geochemical profiles were characterized by a sulfate-to-methane transition with decreasing total organic carbon and nitrogen in sediments, and increasing dissolved inorganic carbon, ammonium and total sulfur in sediments. Bacterial and archaeal 16S rRNA and amoA gene abundances decreased with depth. In contrast, ANME-1 and -2 16S rRNA gene abundances increased significantly across the sulfate-to-methane transition and peaked at different horizons below this interface. A total of 124,379 bacterial and 130,351 archaeal reads were recovered through tag-pyrosequencing of 16S rRNA genes and categorized into 9014 bacterial and 6394 archaeal operational taxonomic units on the basis of 97% sequence similarity, respectively. Major bacterial phyla/divisions and archaeal groups (>5% of the total reads) detected included Chloroflexi, Planctomycetes, OP9, Deltaproteobacteria, BHI80-139, MBG-B, Halobacteria, MCG, Thermoplasmata, ANME-1 and MG-I. The abundance variations of most major OTUs (>0.5% of the total reads) were statistically correlated with those of geochemical parameters. These lines of evidence suggest that the populations represented by the major OTUs or detected by group-specific primers were compartmentalized into different horizons and involved directly or indirectly in the cycling of methane, sulfate, organic carbon and nitrogen. Overall, this study demonstrates that the deep sequencing coverage combined with the quantification of gene abundance and geochemical characterization would enable to uncover the detailed distributions and potential metabolic capabilities of specific groups from complexly structured microbial communities in methane-rich marine sediments.  相似文献   

中国海相油气田勘探实例之十四 珠江口盆地深水区流花16-2轻质油田的发现与勘探     

孙金山  刘鑫  杜克拯  蒋钱涛 《海相油气地质》2013,(1):71-78

流花16-2油田发现于2010年5月。它是南海北部珠江口盆地近年发现的中型轻质油田,也是该区自营勘探以来发现的储量最大的油田。油田位于珠江口盆地番禺低凸起东端海域深水区,流花16-2构造为断块圈闭,储层为下中新统珠江组下段及上渐新统珠海组海相中细砂岩和生物碎屑灰岩,盖层为珠江组上段巨厚层海相泥岩,具有良好的储盖组合。油源来自油田南面的白云凹陷东凹古近系恩平组—文昌组烃源岩。油藏具有埋深浅、储量丰度高、原油密度低、测试产能高等特点。论述了油田的发现与勘探历程以及油田的基本地质特征,总结了取得勘探突破的实践认识与启示。  相似文献   

养殖牙鲆鱼苗腹水症病原菌的鉴定及系统发育学分析   总被引：34，自引：7，他引：34       下载免费PDF全文

莫照兰  茅云翔  陈师勇  张振冬  徐永立  张培军 《海洋与湖沼》2003,34(2):131-141

从患腹水症养殖牙鲆鱼苗分离到一株细菌B17，人工感染实验证实春具有致病性。用Biolog-GN细菌鉴定系统对B17进行测试，发现其与参考菌株的相似性较低，不能进行鉴定。常规生化测试表明，B17具有弧菌属的特征，其表型特征与Vibrio splendidus非常相似。为了进一步明确菌株B17的分类学地位，测定了其16SrRNA基因序列，分析了相关细菌相应序列的同源性，构建了系统发生树，结果表明菌株B17与V. splendidus的亲缘关系最近。综合上述结果，菌株B17可鉴定为V.splendidus。  相似文献   

漠河盆地科学钻探井MK-1地下冷环境中嗜热微生物的多样性分析   总被引：1，自引：1，他引：0  

李建华  张亚丽  吕杰  杨红兵 《冰川冻土》2012,34(3):726-731

在漠河盆地科学钻探井MK-1地下110m冷环境样品中发现大量嗜热微生物存在的证据,针对这些冷环境中的嗜热菌进行了克隆文库分析和分离培养,分别用细菌和古菌16SrDNA引物PCR扩增.结果表明:未发现古菌16SrDNA存在,而嗜热细菌与栖热菌属(Thermus),地芽孢杆菌属(Geoba-cillus)和厌氧芽孢杆菌属(Anoxybacillus)有较近的亲缘关系,通过分离培养分离出3种地芽孢杆菌属(Geobacillus)的细菌,其相似度均为100%.嗜热微生物多存在于地下温暖有机质丰富的生态系统中,了解它们的来源、分布机制及其多样性对于能源和资源的寻找在一定程度上具有指示意义.  相似文献   

Screening for and Identification of an Anti‐clam Vibrio Marine Bacterium from an Aquaculture Pond in the Yellow Sea          下载免费PDF全文

Xing Sun  Gengmao Zhao  Chenhao Gu  Ling Liu  Ming Zhu  Zhaopu Liu 《洁净——土壤、空气、水》2016,44(3):304-308

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Oceanic sound channels around New Zealand     

D. M. Garner 《新西兰海洋与淡水研究杂志》2013,47(1):3-15

Configuration of major sound channels in the ocean around New Zealand is derived from the temperature and salinity data available from the region between latitudes 28°S and 56°S and between 158°E and 174°W. The “SOFAR channel” is established throughout the area northwards of the Antarctic Convergence, with its axis in a depth of about 1,300 m. Little variation in the depth of this axis was found except in the southern part of the subantarctie zone, where the weak vertical temperature stratification cannot maintain a velocity minimum; the axis of the SOFAR channel tends to decrease in depth as it loses its identity. In the northern part of the subantarctie region, a second channel was found at a depth of about 100 m. The depth of the axis of this “subantarctie channel” increases to about 500 m under the surface outcrop of the Subtropical Convergence. It loses its identity at about 400 m in the stronger vertical temperature stratification of the subtropical region. To the south of the Antarctic Convergence, an “antarctic channel” was found with its axis at a depth of some 400 m in a temperature inversion between Antarctic Winter Water and the underlying Pacific Deep Water.

Sound velocity on the surfaces defined by the channel axes is mapped. A ridge of maximum SOFAR velocity is defined extending east and west of the northern part of New Zealand. This feature does not seem to appear in the north Pacific and has been tentatively associated with the dynamics of east‐flowing subtropical currents in this region.  相似文献   

基于线粒体16S rRNA 基因研究5 个栉江珧野生群体的遗传多样性   总被引：1，自引：0，他引：1  

严加坤  杨爱国  周丽青  吴彪  白临建  刘志鸿 《海洋科学》2013,37(2):36-42

采用通用引物对山东长岛、文登、日照、广东湛江和海南三亚等5个地理群体栉江珧(Atrina pectinta)16S rRNA序列进行扩增、测序分析,得到59条441bp的核苷酸序列.其中T、C、A、G和A+T的平均含量分别为29.91%、17.41%、25.74%、26.94%及55.65%, AT含量高于GC含量,共检测到了9个单倍型和26个核苷酸多态位点.群体多样性分析表明,文登群体具有较高的遗传多样性水平.AMOVA 分析表明,五群体间总遗传分化系数Fst =0.5007(P<0.001),群体间遗传分化略大于群体内、群体间存在较高的遗传分化.基于群体间遗传距离构建NJ和UPGMA分子进化树,5个地理群体的栉江珧聚为两个支,长岛、文登、日照群体聚为一支,海南和湛江群体聚为另一支.  相似文献   

荣成天鹅湖大叶藻(Zostera marina)间2种小型腹足类的分类学研究     

刘静  管洪在  张素萍  郭小芳  杨学伶  王海艳 《海洋与湖沼》2013,44(1):226-234

小型腹足类生物在我国种类繁多,但其相关研究在国内基本属于空白.本文对荣成天鹅湖大叶藻间的2种小型腹足类生物进行了形态学及分子生物学(16S和28S rRNA基因片段)研究.结果表明,根据形态学所鉴定的短剑螺属未定种Machaeroplax sp.,应为畦螺属未定种Lirularia sp.;分子生物学研究结果支持形态学对刺绣翼螺Alaba picta的鉴定.同时,与A.picta有一定形态学差异的其它4种相近形态的小型腹足类,在基因序列及遗传距离上与刺绣翼螺非常接近,亦应为刺绣翼螺Alaba picta.  相似文献   

不同养殖区红藻表面假交替单胞菌多样性分析   总被引：2，自引：0，他引：2  

武洪庆  刘敏  肖天 《海洋科学》2013,37(10):17-23

利用2216E培养基从我国沿海6种养殖红藻的20个样品表面分离了327 株假交替单胞菌。经过16S rDNA序列鉴定, 分别隶属于Pseudoalteromonas carrageenovora、P. haloplanktis、P. marina、P. agarivorans、P. elyakovii和P. lipolytica 6个种。其中P. carrageenovora数量最多, 总共211株, 在14个样品上均有发现。  相似文献