TIME-HARMONIC VIBRATION OF AN INCOMPRESSIBLE LINEARLY NON-HOMOGENEOUS HALF-SPACE     

G. MURAVSKII  V. OPERSTEIN 《地震工程与结构动力学》1996,25(11):1195-1209

In this paper, time-harmonic axisymmetric vibration of an incompressible viscoelastic half-space having shear modulus linearly increasing with depth is studied. The half-space is subjected to a vertical time-harmonic load on its surface. Numerical results concerning surface displacements due to a point force are given for the case of non-zero shear modulus at the surface. Hankel's transforms of the solutions have an infinite number of poles lying on the real axis of the complex plane in the non-dissipative case. A suitable contour of integration is used to avoid all the singularities. Calculations are performed for the dynamic as well as for the static case. In addition, vertical vibrations of a rigid disk on the considered half-space are investigated, and the influence of the non-homogeneity on the dynamic stiffness of the loaded area is demonstrated.  相似文献   

超声与16层螺旋CT在小肝癌诊断中的应用研究   总被引：2，自引：0，他引：2  

戴琳  许乙凯  冯筱榕 《CT理论与应用研究》2006,15(2):52-55

目的:应用超声和16层螺旋CT进行小肝癌联合诊断的对照研究。方法:采用常规超声及高质量扫描模式的16层螺旋CT对18例小肝癌和17例肝脏良性小肿瘤进行对照诊断研究,所有病例均经手术病理或超声引导下穿刺证实。结果:大部分肝脏良恶性小肿瘤在超声图像上有其典型的特征,与CT和病理检查结果相符的有小肝癌14例和良性小肿瘤15例。但,超声诊断怀疑为血管瘤1例和增生结节3例,CT和病理诊断为小肝癌;超声误诊为小肝癌2例而CT和病理均诊断为硬化结节1例,另1例CT诊断与超声相符,但病理确证后为炎性假瘤。结论:超声是肝脏常规检查的首选方法,对肝脏良恶性肿瘤均有较高的检出率,但对一些不典型病例还需16层螺旋CT和病理检查以做出进一步的定性诊断,提高诊断的准确率,二者联合诊断能提高对肝脏良恶性肿瘤的早期诊断率。  相似文献   

中国沿海脉红螺(Rapana venosa)自然群体线粒体 DNA 16S rRNA遗传特性研究   总被引：7，自引：1，他引：6  

杨建敏  李琪  郑小东  宋志乐  王如才 《海洋与湖沼》2008,39(3):257-262

对中国沿海脉红螺7个自然居群的线粒体DNA 16S rRNA基因片段进行了扩增和测序,分析了107个个体511bp的碱基序列,结果没有发现插入,缺失突变的核苷酸位点.检测到了28个多态性核苷酸位点,共37种单倍型,单倍型1的同源性达到58.7%.大连居群的28l(G-A)和483(T- C)位点,烟台居群的17(A-G)位点、66(T- C)和247(T-C)位点,舟山居群的128(T-C)位点,秦皇岛居群的130(T-G)和161(A-G)位点,以及丹东居群的431(T-C)位点的变异可以作为居群分子遗传标记位点.居群内,烟台和大连居群的核苷酸差异数 K 以及平均核苷酸多样性指数 Pi 最高,青岛居群最低;居群间,大连居群在居群间核苷酸差异数K、居群间平均每位点核苷酸替代数Dxy 和居群间每位点净核苷酸替代数Da三个指标上都表现出比其他居群之间较高的水平,说明大连居群具有最为丰富的遗传多样性.  相似文献   

Colonization of Sulfurovum sp. on the gill surfaces of Alvinocaris longirostris, a deep-sea hydrothermal vent shrimp     

Gaku Tokuda  Akinori Yamada  Kazuma Nakano  Nao O. Arita  & Hideo Yamasaki 《Marine Ecology》2008,29(1):106-114

Deep‐sea hydrothermal vents are unique light‐independent ecosystems that are sustained by chemosynthetic bacteria. For many of the invertebrates inhabiting in such environments, bacteria play essential roles in both energy acquisition and detoxification of potentially toxic gases such as H₂S. In this study, the bacterial flora present on the gills of Alvinocaris longirostris (Bresiliidae: Caridea), a shrimp inhabiting hydrothermal vents (1532 m depth) at the Hatoma Knoll of the Okinawa Trough, was investigated. Bacterial 16S rDNA fragments were successfully amplified from the gills and 70% of these fragments showed an identical pattern in the restriction fragment length polymorphism analysis. These fragments were assigned to the ribotype AL‐1. Phylogenetic analyses suggest that AL‐1 forms a monophyletic clade with Sulfurovum spp. (ε‐Proteobacteria). Fluorescence in situ hybridization for AL‐1 and electron microscopy showed the presence of short‐rod bacteria lining up on the cuticular layer of the surface of the gill filaments. These results suggest that bacterial association with gills also occurs in bresiliid shrimps.  相似文献   

海洋石油烃降解细菌T1和T2的分子鉴定分类     

田胜艳  王娟  聂利红 《海洋科学》2008,32(9)

从天津滨海潮间带被石油烃严重污染的沉积物（千样含油量0．2g／g）中,筛选分离出能够以柴油为唯一碳源生长的细菌,对其中生物量大、单株菌降解效率较高的两株细菌T1和T2进行16SrDNA克隆,通过测定和比较16SrDNA的部分序列对这两株细菌进行分子鉴定,以期用于石油污染的微生物修复中。结果表明,T1与深红红螺菌（Rhodospirillum rubrum）的同源性为89％,．r2与施氏甲单孢杆菌（Pseudomonas stutzeri）的同源性为99％。  相似文献   

一株海水氨氧化细菌的系统发育分析     

黄建军  简纪常  吴灶和  丁燏 《广东海洋大学学报》2008,28(3)

应用PCR技术扩增16S rRNA基因和amoA(ammonia monooxygenase subunit A)的基因片段,并测定其序列,对一株源于海水养殖水体的高效氨氧化细菌(ammonia-oxidizing bacteria,AOB)进行了系统发育分析。结果表明,经PCR扩增得到了1 098 bp的16S rRNA基因片段和491 bp的amoA基因片段,将其序列用NCBI-Blast软件在GenBank数据库中进行同源性检索后发现,该菌株的16S rRNA基因序列和amoA基因序列分别与亚硝化单胞菌Nitrosomonas sp.NS20的相对应基因片段相似性分别为98.4%和96.7%。在此基础上构建了系统发育树,表明用该菌株的16S rRNA基因片段和amoA基因片段构建的系统发育树均与亚硝化单胞菌属类聚在一起,结合该菌株形态和生理生化特性,鉴定该株氨氧化细菌属亚硝化单胞菌。  相似文献   

Isolation and diversity analysis of heterotrophic bacteria associated with sea anemones   总被引：2，自引：0，他引：2  

DU Zongjun  ZHANG Wanyi  XIA Hongjie  L&#; Guoqiang  CHEN Guanjun 《海洋学报(英文版)》2010,29(2):62-69

A study was undertaken to investigate the heterotrophic bacterial flora associated with the sea anemones. Samples of the sea anemones Anthopleura midori were collected from the coast of Weihai and bacteria were isolated from these samples. Additionally, high numbers of viable bacteria were obtained from the celom wall and surface of anemone, the community of cultivable bacteria was very diverse. As a result of this isolation, 60 strains were obtained, 56 of them were selected for identification and characterization by 16S rRNA gene sequence analysis and limited phenotypic testing. Among these isolates, 16 strains were phylogenetically related to members of the genus Pseudoalteromonas and neighboring taxa. Other isolates included members of the genera Colwellia, Vibrio, Acinetobacter, Pseudomonas, Endozoicomonas, Roseovarius, Paracoccus, Loktanella, Leisingera, Sulfitobacter, Bacillus, Staphylococcus, Plantibacter, Microbacterium, Micrococcus, Joostella, Psychroserpens, Cellulophaga, Krokinobacter, Polaribacter and Psychrobacter. Seven potential novel species were found. Among 60 strains, 17 of them can produce proteolytic exoenzyme, 20 can produce lipolytic exoenzyme. Strain NQ8 has strong antagonistic effects on some Vibrio strains. This study demonstrates that the culturable fraction of bacteria from the sea anemones Anthopleura midori is diverse and appears to possess much potential as a source for the discovery of novel bioactive materials.  相似文献   

印度洋深海热液区可培养细菌的分子鉴定与系统发育分析   总被引：1，自引：0，他引：1  

刘艳  黄晓航  何培青  彭亚林  林学政  安利国 《海洋科学进展》2009,27(2)

采用MMJHS寡营养培养基从印度洋深海热液区沉积物和热液硫化物中分离获得16株细菌,通过16S rDNA序列比对和生理生化分析,对它们进行了鉴定并构建了系统发育树.结果表明,12株细菌属于γ-变型菌(γ-Gammaproteobacteria),其中6株属于盐单胞菌属(Halomonas),4株属于嗜冷杆菌属(Psychrobacter),2株属于食碱菌属(Alcanivorax);其余4株属于芽孢杆菌(Bacillus),其中1株为地衣芽孢杆菌(Bacillus licheniformis).分离获得的16株细菌中,4株为革兰氏阳性细菌,12株为革兰氏阴性细菌;H2S反应、吲哚测定反应、M.R和V-P均呈阴性.本研究为深入认识和开发利用深海热液区微生物资源奠定了基础.  相似文献   

基于ARM和SD卡的微动数据采集系统数据存储技术研究   总被引：1，自引：0，他引：1  

陈卫明  廖成旺  邓涛  丁炜  王浩 《大地测量与地球动力学》2009,29(Z1):114-119

为满足微动数据采集系统的大数据量、低功耗要求,用SD卡存储微动数据采集系统采集得到的数据。阐述如何实现SD卡在以三星ARM9-s3c2410为硬件平台、Linux操作系统为软件平台的微动数据采集系统中的存储功能。  相似文献   

16S rRNA Gene Phylogenesis of Culturable Predominant Bacteria from Diseased Apostichopus japonicus(Holothuroidea,Echinodermata)     

MA Haiyan  JIANG Guoliang  WU Zhiqiang  WANG Xin 《中国海洋大学学报(英文版)》2009,8(2)

Cultured Apostichopusjaponicus in China suffers from a kind of skin ulceration disease that has caused severe economic loss in recent years. The disease, pathogens of which are supposed to be bacteria by most researchers, is highly infectious and can often cause all individuals in the same culture pool to die in a very short time. The 16S rRNA gene phylogenesis of the culturable bacteria from the lesions of diseased individuals was conducted to study the biodiversity of the bacterial communities in the lesions and to identify probable pathogen(s) associated with this kind of disease. S. japonica samples were selected from a hatchery located in the eastern part of Qingdao, China. Bacterial universal primers GM5F and DS907R were used to amplify the 16S rRNA gene of bacteria colonies, and touchdown PCR was performed to amplify the target sequences. The results suggest that γ- proteobacteria (Alteromonadales and Vibrionales) of CFB group, many strains of which have been also determined as pathogens in other marine species, are the predominant bacterial genera of the diseased Apostichopusjaponicus individuals.  相似文献