不同氮源对微小亚历山大藻生长和毒素产生的影响   总被引：12，自引：0，他引：12  

张清春  于仁诚  周名江  王云峰  颜天  李钧 《海洋学报》2005,27(6):138-145

通过尿素、氯化铵、酵母浸出粉和硝酸钠等氮源对微小亚历山大藻(Alexandrium minutum)生长及毒素产生的影响研究,分析了微小亚历山大藻对不同氮源利用状况的差异.结果表明,在氮饥饿条件下,加入硝酸钠和酵母浸出粉能显著促进微小亚历山大藻的生长;高浓度的氯化铵在加入后对微小亚历山大藻有一定的毒性效应,表现为生长停滞,但毒性效应在5 d后消失,并得到与添加硝酸钠及酵母浸出粉相似的增长速率0.21 d-1;添加尿素对微小亚历山大藻的生长没有显著促进作用.在四种氮源中,尿素对微小亚历山大藻毒素产生的刺激作用也最弱,在稳定期每个细胞藻细胞毒素含量维持在6.00~8.00 fmol;添加硝酸钠、氯化铵和酵母浸出粉的藻细胞在稳定期毒素含量分别达到11.85,12.86和14.64 fmol.硝酸钠和氯化铵刺激藻毒素产生的效果比酵母浸出粉更为直接.四种含氮营养盐对微小亚历山大藻毒素组成的影响都很小.  相似文献   

黑鲷(Acanthopagrus schlegeli)肿瘤坏死因子α cDNA的克隆及特征分析   总被引：1，自引：0，他引：1  

高春萍  蔡中华  宋林生  吴龙涛  池振明 《海洋与湖沼》2005,36(4):326-334

采用同源克隆的方法，从黑鲷中获得肿瘤坏死因子(TNFα)全长cDNA序列。该序列含1288个核苷酸，可编码253个氨基酸的TNF前体蛋白。它是一种跨膜蛋白，无糖基化位点和信号肽结构。黑鲷TNFα与其它鱼类的TNFα的相似性很高，占据进化上独立的分支；与哺乳类TNFα和TNFG源于共同的祖先。基因结构分析显示，该基因含有TNF家族pmfile和TNF家族的标签序列；在参与TNFα基因二硫键形成的两个半胱氨酸和TNFα三聚体形成的位点高度保守；空间结构模拟显示，它与哺乳类TNFα的空间结构相似，都是由两个β折叠片组成，每个折叠片包含5个反向平行的β链。表达研究结果表明，黑鲷TNFα在检测的各个组织中均为组成型表达，表现为在刺激与非刺激鱼体中，都可以检测到黑鲷TNFα的表达，但是其表达水平在不同组织中有很大差异。黑鲷TNFα在头肾、脾脏和鳃的表达量较高，而在心脏、肝脏、血液、肾脏和大脑中表达量较低。  相似文献   

藓羽藻(Bryopsis hypnoides)核酮糖-1，5-二磷酸羧化酶／加氧酶的分离与活性测定     

田超  张炎  王广策  曾呈奎 《海洋与湖沼》2004,35(4):371-375

采用硫酸铵分部沉淀与凝胶过滤的方法，进行藓羽藻Rubisco的分离研究。结果表明，分离的藓羽藻Rubisco经SDS-聚丙烯酰胺凝胶电泳检测呈两条清晰条带，分别为Rubisco大亚基与小亚基；与菠菜相比，藓羽藻Rubisco大亚基分子量与菠菜基本相同，而小亚基较之稍大一些。藓羽藻Rubisco活力测定结果表明，Rubisco分离过程中用硫酸铵分部沉淀后活力降低许多，分离后活力有所上升，但仍比粗提液活力弱；在Rubisco活力测定过程中，藓羽藻Rubisco的活化温度与其它物种Rubisco活化的温度不同，在低温下活化效果较好。这些结果说明Rubisco的酶活力受硫酸铵的影响而且藓羽藻Rubisco相对陆地高等植物结构不稳定。  相似文献   

Patterns and controls of surface sediment distribution: west-central Florida inner shelf   总被引：2，自引：0，他引：2  

Gregg R. Brooks  Larry J. Doyle  Richard A. Davis  Nancy T. DeWitt  Beau C. Suthard   《Marine Geology》2003,200(1-4):307-324

The west-central Florida inner shelf represents a transition between the quartz-dominated barrier-island system and the carbonate-dominated mid-outer shelf. Surface sediments exhibit a complex distribution pattern that can be attributed to multiple sediment sources and the ineffectiveness of physical processes for large-scale sediment redistribution. The west Florida shelf is the submerged extension of the Florida carbonate platform, consisting of a limestone karst surface veneered with a thin unconsolidated sediment cover. A total of 498 surface sediment samples were collected on the inner shelf and analyzed for texture and composition. Results show that sediment consists of a combination of fine quartz sand and coarse, biogenic carbonate sand and gravel, with variable but subordinate amounts of black, phosphorite-rich sand. The carbonate component consists primarily of molluskan fragments. The distribution is patchy and discontinuous with no discernible pattern, and the transition between sediment types is generally abrupt. Quartz-rich sediment dominates the inner 15 km north of the entrance into Tampa Bay, but south of the Bay is common only along the inner 3 km. Elsewhere, carbonate-rich sediment is the predominate sediment type, except where there is little sediment cover, in which cases black, phosphorite-rich sand dominates. Sediment sources are likely within, or around the periphery of the basin. Fine quartz sand is likely reworked from coastal units deposited during Pleistocene sea-level high stands. Carbonate sand and gravel is produced by marine organisms within the depositional basin. The black, phosphorite-rich sand likely originates from the bioerosion and reworking of the underlying strata that irregularly crop out within the study area. The distribution pattern contains elements of both storm- and tide-dominated siliciclastic shelves, but it is dictated primarily by the sediment source, similar to some carbonate systems. Other systems with similar sediment attributes include cool-water carbonate, sediment-starved, and mixed carbonate/siliciclastic systems. This study suggests a possible genetic link among the three systems.  相似文献   

无人机载线阵摆扫CCD影像几何校正     

张艳  王涛  赵亮  刘颖  徐青  孙雷 《海洋测绘》2006,26(5):65-67,78

针对无人机载线阵CCD传感器摆扫成像的技术特点,利用GPS/INS组合导航系统提供的导航遥测数据,对无人机载的线阵摆扫CCD影像建立了严格的成像模型,并设计了详细的几何校正流程。实验结果证实采用文中的成像模型和几何校正流程,可获得正确、高精度地理编码的线阵摆扫CCD正射影像。  相似文献   

A comparison of three marine ecosystems surrounding the Korean peninsula: Responses to climate change     

Ginger A. Rebstock  Young Shil Kang 《Progress in Oceanography》2003,59(4):357-379

This study uses a comparative approach to examine responses of marine ecosystems to climatic regime shifts. The three seas surrounding the Korean peninsula, the Japan/East Sea, the East China Sea and the Yellow Sea represent three contiguous but distinct ecosystems. Sampling has been carried out by the National Fisheries Research and Development Institute of South Korea since 1965, using the same methods in all three seas. Sampling was generally synoptic. Amplitude time series of 1st EOF modes for temperature, salinity, zooplankton biomass and concentrations of four major zooplankton taxa were used to determine whether the three marine ecosystems respond in a similar manner to climate variations. Temporal patterns of the variables were strongly similar among the three seas at decadal time scales, but very weakly similar at interannual scales. All three seas responded to a climatic regime shift that occurred in 1989. Temperature, zooplankton biomass and copepod concentrations increased in the late 1980s or early 1990s in all three seas. Concentrations of amphipods, chaetognaths and euphausiids also increased in the Japan/East Sea and the East China Sea, but not the Yellow Sea. The Yellow Sea ecosystem differs strongly from the other two seas, and water exchange between the Yellow Sea and the East China Sea is much weaker than that between the East China Sea and Japan/East Sea. Spatial patterns of zooplankton determined by the EOF analysis were closely related to currents and fronts in each of the three seas.  相似文献   

渤海大型底栖动物次级生产力的初步研究   总被引：12，自引：2，他引：12  

于子山  张志南  韩洁 《中国海洋大学学报(自然科学版)》2001,31(6):867-871

1998年 9月和 1999年 4月分别对渤海 2 0个站进行了大型底栖动物两个航次的调查取样。整个研究海域大型底栖动物年次级生产力平均值为 6 .4 9g(AFDW) /(m2 · a) ,其中渤海海峡以东海域较高 ,为 12 .5 9g(AFDW) /(m2· a) ;渤海中部为 4 .4 6 g(AFDW) /(m2· a) ,渤海大型底栖动物年平均 P/B为 0 .82  相似文献   

Analytical chemistry in monitoring the effects of aquaculture: one laboratory's perspective     

Zitko  V. 《ICES Journal of Marine Science》2001,58(2):486-491

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基于强跟踪性能的QEKF组合导航自适应滤波研究     

夏琳琳  赵琳  程建华 《测绘学报》2008,37(4):410-417

以低成本陆用航姿参考系统（AHRS）/GPS紧耦合系统为研究对象,建立了基于伪距、伪距率、航向角的组合观测数学模型。引入强跟踪滤波（STF）算法,利用渐消因子的作用,增强滤波器对状态快速变化的跟踪能力。同时,针对微惯性器件漂移过大的缺点,采用二阶EKF（QEKF）方法,通过对Hessian矩阵的求解,补偿系统观测方程线性化的二阶截断误差。仿真表明：STQEKF方法可高速准确地逼近系统非线性模型,实现陆地载体导航控制,在传感器精度有限的情况下,使姿态和位置的控制效果较标准EKF分别提高了约8.9%-38.2%和48.7%-54.4%  相似文献   

城市景观三维重建中的三角剖分算法   总被引：1，自引：1，他引：0  

殷勇  俞连芳  马照亭  李成名 《测绘科学》2008,33(4)

在构建城市景观时,为了快速精确地重建建筑物、绿地、河流等三维模型,提高自动化程度,缩短建设周期,需对用户采集的矢量地物数据进行三角剖分,再以OpenGL重绘三维模型。本文提出了一种对多边形进行三角剖分的递归分割算法,并以VisualC++实现,以实际的4万多个建筑物进行三角剖分实验,充分证明其是有效的。  相似文献